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基于指纹图谱及网络药理学的百合质量标志物研究

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药物分析杂志 2024年第5期44卷 893-902页

作者：赵永琪张宏伟张振凌李雅静王一鸣河南中医药大学豫药全产业链研发河南省协同创新中心河南省中药特色炮制技术工程研究中心河南省中药生产一体化工程技术研究中心郑州450046

目的:通过指纹图谱、多成分含量测定和网络药理学对百合质量标志物进行分析,为确定百合饮片的质量标志物提供依据。方法:采用HPLC法建立15批百合饮片的指纹图谱,对共有峰进行化学计量学分析,筛选出差异成分;对差异成分进行多成分含量测... 详细信息

目的:通过指纹图谱、多成分含量测定和网络药理学对百合质量标志物进行分析,为确定百合饮片的质量标志物提供依据。方法:采用HPLC法建立15批百合饮片的指纹图谱,对共有峰进行化学计量学分析,筛选出差异成分;对差异成分进行多成分含量测定,比较不同产地的区别,基于网络药理学分析百合差异成分发挥抗抑郁药效的主要途径,并将差异成分进行体外细胞抗抑郁实验,最后基于质量标志物筛选原则分析百合饮片的Q-Marker。结果:15批百合饮片确定了16个共有峰,相似度均>0.931,指认了其中7个共有峰,利用化学计量学筛选出4个差异成分;测定15批百合饮片中王百合苷A、王百合苷B、王百合苷C 3个成分的含量;网络药理学研究发现差异成分可能通过TNF、GAPDH、MAPK3等靶点调节氧化应激反应,保护神经元突触结构,促进单胺类神经递质含量增加等途径来发挥抗抑郁作用;细胞实验结果显示,与模型组相比,百合提取物低、中、高剂量组细胞活力显著提高(P<0.01),王百合苷A、王百合苷B、王百合苷C低、中、高剂量组细胞活力显著提高(P<0.01)。结论:通过指纹图谱和网络药理学研究,建议将王百合苷A、王百合苷B、王百合苷C作为百合饮片的质量标志物,可为百合质量控制和药效研究提供参考。

关键词：百合质量标志物指纹图谱多成分含量测定网络药理学抗抑郁

不同产地及加热方式文冠果仁中的氨基酸成分分析

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中南药学 2024年第4期22卷 1070-1075页

作者：段佩利院军张帅郭幸张振凌河南中医药大学第三附属医院郑州450046 河南中医药大学药学院郑州450046

目的分析不同产地的文冠果仁氨基酸成分及加热方式对氨基酸的影响。方法采用ZORBAX Eclipse Plus C18柱(4.6 mm×250 mm,5μm),样品以异硫氰酸苯酯进行柱前衍生化,以乙腈-甲醇-水(3∶1∶1)为流动相A,0.1 mol·L^(-1)的醋酸钠溶液(p H ... 详细信息

目的分析不同产地的文冠果仁氨基酸成分及加热方式对氨基酸的影响。方法采用ZORBAX Eclipse Plus C18柱(4.6 mm×250 mm,5μm),样品以异硫氰酸苯酯进行柱前衍生化,以乙腈-甲醇-水(3∶1∶1)为流动相A,0.1 mol·L^(-1)的醋酸钠溶液(p H 6.5)-乙腈(93∶7)为流动相B,柱温35℃,检测波长254 nm。结果 17种氨基酸在1.806~225.200μg·m L^(-1)与峰面积呈良好的线性关系,各产地必需氨基酸、药用氨基酸、苦味氨基酸和儿童必需氨基酸占总氨基酸的含量分别为14.40%~18.99%、64.06%~73.79%、14.82%~17.09%、23.53%~29.30%,北方地区的文冠果仁氨基酸含量、必需氨基酸含量和药用氨基酸含量均比较高,文冠果仁经过加热处理后各类氨基酸均有所提高。结论不同产地文冠果仁的氨基酸在含量和营养价值上具有差异,北方地区的文冠果仁氨基酸营养和药用价值较高,文冠果仁经过一定的加热后食用营养价值和药用价值得到提高。

关键词：文冠果氨基酸柱前衍生化异硫氰酸苯酯

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炮制对加味拱辰浓缩丸化学成分的影响

中成药 2024年第3期46卷 755-765页

作者：张亚柯张宏伟张振凌朱建光李德华孙梦梅赵永琪王一鸣河南中医药大学药学院河南郑州450046 呼吸疾病中医药防治省部共建协同创新中心河南郑州450018

目的考察炮制对加味拱辰浓缩丸化学成分的影响。方法采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UHPLC-Q-Orbitrap HRMS)法对生品组(山萸肉+当归)、炮制品组(酒萸肉+酒当归)、酒当归组(酒当归+山萸肉)、酒萸肉组(酒萸肉+当归... 详细信息

目的考察炮制对加味拱辰浓缩丸化学成分的影响。方法采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UHPLC-Q-Orbitrap HRMS)法对生品组(山萸肉+当归)、炮制品组(酒萸肉+酒当归)、酒当归组(酒当归+山萸肉)、酒萸肉组(酒萸肉+当归)样品进行定性鉴别,主成分分析、正交偏最小二乘判别分析筛选差异成分,HPLC法测定莫诺苷、马钱苷、山茱萸新苷、没食子酸、阿魏酸、马钱苷酸、绿原酸、藁本内酯、5-羟甲基糠醛的含量。结果共鉴定出97种成分,包括有机酸、环烯醚萜、苯肽、皂苷、核苷、氨基酸,生品组、炮制品组、酒当归组、酒萸肉组中分别鉴定出90、88、89、86种成分。与生品组和酒当归组比较,酒萸肉组和炮制品组中有机酸总含量升高,环烯醚萜苷总含量接近;酒萸肉组中藁本内酯含量最高。结论酒萸肉的加入可增加加味拱辰浓缩丸组方配伍合理性,酒当归的加入也具有一定协同增效作用。

关键词：加味拱辰浓缩丸化学成分炮制超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UHPLC-Q-Orbitrap HRMS) 主成分分析正交偏最小二乘判别分析 HPLC

Central environmental protection inspection and carbon emission reduction: A tripartite evolutionary game model from the perspective of carbon neutrality

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Petroleum Science 2024年第3期21卷 2139-2153页

作者： zhen-Hua zhang Dan ling Qin-Xin Yang Yan-Chao Feng Jing Xiu School of Economics Lanzhou UniversityLanzhou 730000GansuChina Institute of Green Finance Lanzhou UniversityLanzhou 730000GansuChina School of Management Lanzhou UniversityLanzhou 730000GansuChina School of Business Zhengzhou UniversityZhengzhou 450001HenanChina Institute of Economics Jilin Academy of Social SciencesChangchun130033JilinChina

Since the carbon neutrality target was proposed,many countries have been facing severe challenges to carbon emission reduction *** study is conducted using a tripartite evolutionary game model to explore the impact of the central environmental protection inspection(CEPI)on driving carbon emission reduction,and to study what factors influence the strategic choices of each party and how they interact with each *** research results suggest that local governments and manufacturing enterprises would choose strategies that are beneficial to carbon reduction when CEPI *** the initial willingness of all parties increases 20%,50%—80%,the time spent for the whole system to achieve stability decreases from 100%,60%—30%.The evolutionary result of“thorough inspection,regulation implementation,low-carbon management”is the best strategy for the tripartite evolutionary ***,the smaller the cost and the larger the benefit,the greater the likelihood of the three-party game stability strategy *** study has important guiding significance for other developing countries to promote carbon emission reduction by environmental policy.

关键词： Central environmental protection inspection Local government Manufacturing enterprise Tripartite evolutionary game Carbon emission reduction

Design Research of a Novel Aftercool-Humidifier Concept for Humid Air Turbine Cycle

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Journal of Thermal Science 2024年第3期33卷 951-969页

作者： zhang Junzheng XU zhen School of Energy and Power Engineering Shandong UniversityJi’nan250061China Shandong Engineering Laboratory for High-efficiency Energy Conservation and Energy Storage Technology&Equipment Shandong UniversityJi’nan250061China

Humid air turbine cycle(HAT)has potential of electrical efficiencies comparable to combined cycle,with lower investment cost and NO_(x) *** typical heat exchanger network of HAT consists of intercooler(if there is),aftercooler,recuperator,economizer and humidifier,which brings higher efficiency but makes the system more *** simplify HAT layout,a novel humidifier concept is proposed by integrating the aftercooler into traditional counter-current *** on this concept,a one-dimensional model including pressure drop and exergy calculation is established to distinguish the thermodynamic and hydrodynamic characteristics,and then the structural parameters,such as the number of rows and columns,tube diameter,pitch and type for a micro HAT are *** results show that the aftercool-humidifier plays the same role as original aftercooler and humidifier,and can match the in-tube air,out-tube air and water stream well with lower *** the case of micro HAT cycle,the volume of heat and mass transfer area can be reduced by 47%compared with traditional *** major thermal resistance occurred in the convection heat transfer process inside the tube;however,using enhanced tube cannot effectively improve the compactness of device.

关键词： humid air turbine cycle humidifier thermodynamic analysis one-dimensional model

Patient-derived induced pluripotent stem cells with a MERTK mutation exhibit cell junction abnormalities and aberrant cellular differentiation potential

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World Journal of Stem Cells 2024年第5期16卷 512-524页

作者： Hang zhang ling-Zi Wu zhen-Yu Liu Zi-Bing Jin Beijing Institute of Ophthalmology Beijing Tongren Eye CenterBeijing Tongren HospitalCapital Medical UniversityBeijing 100730China

BACKGROUND Human induced pluripotent stem cell(hiPSC)technology is a valuable tool for generating patient-specific stem cells,facilitating disease modeling,and invest-igating disease ***,iPSCs carrying specific mutations may limit their clinical applications due to certain inherent *** To investigate the impact of MERTK mutations on hiPSCs and determine whether hiPSC-derived extracellular vesicles(EVs)influence anomalous cell junction and differentiation *** We employed a non-integrating reprogramming technique to generate peripheral blood-derived hiPSCs with and hiPSCs without a MERTK ***-somal karyotype analysis,flow cytometry,and immunofluorescent staining were utilized for hiPSC *** and proteomics were employed to elucidate the expression patterns associated with cell junction abnormalities and cellular differentiation ***,EVs were isolated from the supernatant,and their RNA and protein cargos were examined to investigate the involvement of hiPSC-derived EVs in stem cell junction and *** The generated hiPSCs,both with and without a MERTK mutation,exhibited normal karyotype and expressed pluripotency markers;however,hiPSCs with a MERTK mutation demonstrated anomalous adhesion capability and differentiation potential,as confirmed by transcriptomic and proteomic ***,hiPSC-derived EVs were involved in various biological processes,including cell junction and *** HiPSCs with a MERTK mutation displayed altered junction characteristics and aberrant differentiation ***,hiPSC-derived EVs played a regulatory role in various biological processes,including cell junction and differentiation.

关键词： Cell junction Cellular differentiation Extracellular vesicle Human induced pluripotent stem cells Transcriptomics Proteomics

Bayesian network-based survival prediction model for patients having undergone post-transjugular intrahepatic portosystemic shunt for portal hypertension

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World Journal of Gastroenterology 2024年第13期30卷 1859-1870页

作者： Rong Chen ling Luo Yun-Zhi zhang zhen Liu An-Lin Liu Yi-Wen zhang Department of Infectious Diseases Second Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China

BACKGROUND Portal hypertension(PHT),primarily induced by cirrhosis,manifests severe symptoms impacting patient *** transjugular intrahepatic portosystemic shunt(TIPS)is a critical intervention for managing PHT,it carries risks like hepatic encephalopathy,thus affecting patient survival *** our knowledge,existing prognostic models for post-TIPS survival in patients with PHT fail to account for the interplay among and collective impact of various prognostic factors on ***,the development of an innovative modeling approach is essential to address this *** To develop and validate a Bayesian network(BN)-based survival prediction model for patients with cirrhosis-induced PHT having undergone *** The clinical data of 393 patients with cirrhosis-induced PHT who underwent TIPS surgery at the Second Affiliated Hospital of Chongqing Medical University between January 2015 and May 2022 were retrospectively *** were selected using Cox and least absolute shrinkage and selection operator regression methods,and a BN-based model was established and evaluated to predict survival in patients having undergone TIPS surgery for *** Variable selection revealed the following as key factors impacting survival:age,ascites,hypertension,indications for TIPS,postoperative portal vein pressure(post-PVP),aspartate aminotransferase,alkaline phosphatase,total bilirubin,prealbumin,the Child-Pugh grade,and the model for end-stage liver disease(MELD)*** on the above-mentioned variables,a BN-based 2-year survival prognostic prediction model was constructed,which identified the following factors to be directly linked to the survival time:age,ascites,indications for TIPS,concurrent hypertension,post-PVP,the Child-Pugh grade,and the MELD *** Bayesian information criterion was 3589.04,and 10-fold cross-validation indicated an average log-likelihood loss of 5.55 with a standard deviation of *** model’s acc

关键词： Bayesian network Cirrhosis Portal hypertension Transjugular intrahepatic portosystemic shunt Survival prediction model

Dynamic recrystallization mechanism, texture evolution development and mechanical characteristics of a Mg–8.7Gd–4.18Y–0.42Zr magnesium alloy by ECAP

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Progress in Natural Science:Materials International 2024年第2期34卷 376-388页

作者： ling zhang Yinglong Li School of Materials Science and Engineering Northeastern University School of Mechanical and Engineering Ningxia Institute of Science and Technology

Equal channel angle pressing is recognized for its ability to refine alloy grains and alter grain orientation, thereby achieve better mechanical performance of the magnesium alloy. This study investigates the microstructures,dynamic recrystallization mechanism, texture development, and mechanical performance of GW94K(Mg–8.7Gd–4.18Y–0.42Zr wt. %) Mg alloy following ECAP-4 passes at 400°C and 3 mm/min. Results show that when high-temperature deformation is undertaken, twin formation is suppressed while dislocation slip is facilitated, increasing dislocation density during deformation. Following ECAP deformation, the sample displayed higher fracture elongation, TYS, and UTS than the as-solutioned GW94K alloy. In particular, the GW94K alloy performed well mechanically after ECAP-4 passes, with an ultimate TYS of 231 MPa, an UTS of 290 MPa, and an elongation of 14.8 %. DDRX and shear bands induce CDRX, both of which are important in plastic deformation. as well as in modifying microstructure and grain orientation during ECAP deformation.

关键词： Dynamic recrystallization mechanism Equal channel angular pressing Magnesium alloy Mechanical performance Mg-Gd-Y-Zr alloy Texture development

Nuclear charge radius predictions by kernel ridge regression with odd-even effects

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Nuclear Science and Techniques 2024年第2期35卷 94-102页

作者： Lu Tang zhen-Hua zhang Mathematics and Physics Department North China Electric Power UniversityBeijing 102206China Hebei Key Laboratory of Physics and Energy Technology North China Electric Power UniversityBaoding 071000China

The extended kernel ridge regression(EKRR)method with odd-even effects was adopted to improve the description of the nuclear charge radius using five commonly used nuclear *** are:(i)the isospin-dependent A^(1∕3) formula,(ii)relativistic continuum Hartree-Bogoliubov(RCHB)theory,(iii)Hartree-Fock-Bogoliubov(HFB)model HFB25,(iv)the Weizsacker-Skyrme(WS)model WS*,and(v)HFB25**** the last two models,the charge radii were calculated using a five-parameter formula with the nuclear shell corrections and deformations obtained from the WS and HFB25 models,*** each model,the resultant root-mean-square deviation for the 1014 nuclei with proton number Z≥8 can be significantly reduced to 0.009-0.013 fm after considering the modification with the EKRR *** best among them was the RCHB model,with a root-mean-square deviation of 0.0092 *** extrapolation abilities of the KRR and EKRR methods for the neutron-rich region were examined,and it was found that after considering the odd-even effects,the extrapolation power was improved compared with that of the original KRR *** strong odd-even staggering of nuclear charge radii of Ca and Cu isotopes and the abrupt kinks across the neutron N=126 and 82 shell closures were also calculated and could be reproduced quite well by calculations using the EKRR method.

关键词： Nuclear charge radius Machine learning Kernel ridge regression method