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Deletion of Salmonella pathogenicity islands SPI-1, 2 and 3 induces substantial morphological and metabolic alternation and protective immune potential

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Journal of Integrative Agriculture 2025年第1期24卷 272-289页

作者： Gaosong Liu Xuelian Lü Qiufeng Tian Wanjiang zhang Fei Yi yueling zhang Shenye Yu State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China

The Salmonella pathogenicity islands(SPIs) play crucial roles in the progression of Salmonella infection. In this study, we constructed an improved λ Red homologous recombination system to prepare single and triple deletion mutants of 3 prominent SPIs(SPI-1, 2, and 3), aiming at the impact of deletion on morphology, carbon source metabolism, adhesion and invasion capacity, in vivo colonization, and immune efficacy in chicks. Our examination revealed that the surface of the single deletion mutants(SM6ΔSPI1, ΔSPI2, and ΔSPI3) exhibited a more rugged texture and appeared to be enveloped in a layer of transparent colloid, whereas the morphology of the triple deletion mutant(SM6ΔSPI1&2&3) remained unaltered when compared to the parent strain. The carbon metabolic spectrum of the SPI mutants underwent profound alterations, with a notable and statistically significant modification observed in 30 out of 95 carbon sources, primarily carbohydrates(17 out of 30). Furthermore, the adhesion capacity of the 4 mutants to Caco-2 cells was significantly reduced when compared to that of the parent strain. Moreover,the invasion capacity of mutants SM6ΔSPI1 and SM6ΔSPI1&2&3 exhibited a substantial decrease, while it was enhanced to varying degrees for SM6ΔSPI3 and SM6ΔSPI2. Importantly, none of the 4 mutants induced any clinical symptoms in the chicks. However, they did transiently colonize the spleen and liver. Notably, the SM6ΔSPI1&2&3mutant was rapidly cleared from both the spleen and liver within 8 days post-infection and no notable pathological changes were observed in the organs. Additionally, when challenged, the mutants immunized groups displayed a significant increase in antibody levels and alterations in the CD3+CD4+ and CD3+CD8+ subpopulations, and the levels of IL-4 and IFN-γ cytokines in the SM6ΔSPI1&2&3 immunized chicken serum surpassed those of other *** summary, the successful construction of the 4 SPI mutants lays the groundwork for further exploration into the p

关键词： Salmonella pathogenicity islands(SPls) morphology carbon source metabolism pathogenicity immunogenicity live attenuated vaccine

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高度近视黄斑裂孔性视网膜脱离术后黄斑微结构变化及其与视功能的相关性

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国际眼科杂志 2025年第2期25卷 281-285页

作者：杨娜温晓英张月玲中国河北省保定市第一中心医院眼科 071000

目的:观察高度近视黄斑裂孔性视网膜脱离(MHRD)患者术后光学相干断层扫描成像(OCT)及光学相干断层扫描血管成像(OCTA)检查指标的变化特征及其与视功能的相关性。方法:选取2019-01/2023-10于保定市第一中心医院进行治疗的高度近视MHRD患... 详细信息

目的:观察高度近视黄斑裂孔性视网膜脱离(MHRD)患者术后光学相干断层扫描成像(OCT)及光学相干断层扫描血管成像(OCTA)检查指标的变化特征及其与视功能的相关性。方法:选取2019-01/2023-10于保定市第一中心医院进行治疗的高度近视MHRD患者78例为研究对象,均行玻璃体切除联合内界膜填塞术治疗,观察术后1、3、6 mo时患者最佳矫正视力(BCVA)与OCT、OCTA指标变化特征,包括黄斑中心凹脉络膜毛细血管层血流面积(CBFA)、血流密度与椭圆体带缺损直径(DEZA),并采用Pearson相关分析与多元线性回归分析术后不同时间点BCVA与CBFA、DEZA、血流密度的相关性。结果:术后1、3、6 mo时患者BCVA分别为1.75±0.79、1.49±0.53、1.08±0.44,各时间点比较均有差异(均P0.05),CBFA与BCVA(LogMAR)呈负相关(P<0.05);术后3、6 mo时,高度近视MHRD患者的CBFA、血流密度与BCVA(LogMAR)呈负相关,DEZA与BCVA(LogMAR)呈正相关(均P<0.05)。多元线性回归分析显示,术后3、6 mo,CBFA、血流密度及DEZA均为BCVA的重要影响因素(均P<0.05)。结论:高度近视MHRD患者术后视功能逐渐提升,且与术后CBFA、DEZA、血流密度密切相关,建议术后定期监测OCT、OCTA指标变化。

关键词：高度近视黄斑裂孔性视网膜脱离光学相干断层扫描成像光学相干断层扫描血管成像视功能黄斑中心凹脉络膜毛细血管层血流面积椭圆体带缺损直径血流密度

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Environmental,social,and economic assessment of energy utilization of crop residue in China

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Frontiers in Energy 2021年第2期15卷 308-319页

作者： yueling zhang Junjie LI Huan LIU Guangling ZHAO Yajun TIAN Kechang XIE Key Laboratory of Resources and Environmental System Optimization(Ministry of Education) College of Environmental Sciense and EngineeringNorth China Electic Power UniversityBeijing 102206China School Chemical and Environmental Engineering China University of Mining and TechnologyBeijng 100083China Department of Energy Conversion and Storage Technical University of DenmarkLyngby 2820Denmark National Institute of Clean and Low Carbon Energy Beijing 102211China Chinese Acadamy of Engineering Beijing 100088China

This paper aims to discuss an environmental, social, and economic analysis of energy utilization of crop residues from life cycle perspectives in China. The methodologies employed to achieve this objective are environmental life cycle assessment (E-LCA), life cycle cost (LCC), and social life cycle assessment (S-LCA). Five scenarios are developed based on the conversion technologies and final bioenergy products. The system boundaries include crop residue collection, transportation, pre-treatment, and conversion process. The replaced amounts of energy are also taken into account in the E-LCA analysis. The functional unit is defined as 1 MJ of energy produced. Eight impact categories are considered besides climate change in E-LCA. The investment capital cost and salary cost are collected and compared in the life cycle of the scenarios. Three stakeholders and several subcategories are considered in the S-LCA analysis defined by UNEP/SETAS guidelines. The results show that the energy utilization of crop residue has carbon emission factors of 0.09–0.18 kg (CO2 eq per 1 MJ), and presents a net carbon emissions reduction of 0.03–0.15 kg (CO2 eq per 1 MJ) compared with the convectional electricity or petrol, but the other impacts should be paid attention to in the biomass energy scenarios. The energy utilization of crop residues can bring economic benefit to local communities and the society, but the working conditions of local workers need to be improved in future biomass energy development.

关键词： crop residue life cycle assessment life cycle cost social life cycle assessment energy production

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Isolation of marine benzo[a]pyrene-degrading Ochrobactrum sp. BAP5 and proteins characterization

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Journal of Environmental Sciences 2009年第10期21卷 1446-1451页

作者： WU Yirui HE Tengteng ZHONG Mingqi zhang yueling LI Enmin HUANG Tongwang HU Zhong Department of Biology Science College Shantou University Shantou 515063 China. Department of Biochemistry and Molecular Biology Medical College Shantou University Shantou 515063 China

A bacterial strain BAP5 with a relatively high degradation ability of benzo[a]pyrene （BaP） was isolated from marine sediments of Xiamen Western Sea, China and identified as Ochrobactrum sp. according to 16S rRNA gene sequence as well as Biolog microbial identification system. Strain BAP5 could grow in mineral salt medium with 50 mg/L of BaP and degrade about 20% BaP after 30 d of incubation. Ochrobactrum sp. BAP5 was able to utilize other polycyclic aromatic hydrocarbons （PAHs）（such as phenanthrene, pyrene and fluoranthene） as the sole carbon source and energy source, suggesting its potential application in PAHs bioremediation. The profile of total soluble protein from Ochrobactrum sp. BAP5 was also investigated. Some over- and special-expressed proteins of strain BAP5 when incubated with the presence of BaP were detected by two-dimensional polyacrylamide gel electrophoresis, and found to be related with PAHs metabolism, DNA translation, and energy production based on peptide fingerprint analysis through matrix-assisted laser desorption/ionization-time of flight mass spectrometry.

关键词： benzo[a]pyrene biodegradation Ochrobactrum sp. BAP5 proteins

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Identification and Characterization of Gene SpDMRT99B and Its Sex-Biased Expression Profile in the Mud Crab,Scylla paramamosain

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Journal of Ocean University of China 2021年第6期20卷 1495-1504页

作者： zhang Yin FANG Shaobin LIN Fei LI Shengkang ZHENG Huaiping zhang yueling IKHWANUDDIN Mhd MA Hongyu Guangdong Provincial Key Laboratory of Marine Biotechnology Institute of Marine SciencesShantou UniversityShantou 515063China STU-UMT Joint Shellfish Research Laboratory Shantou UniversityShantou 515063China Institute of Tropical Aquaculture Universiti Malaysia TerengganuKuala Terengganu 21030Malaysia

The doublesex and mab-3 related transcription factor(DMRT)gene family is conserved from invertebrates to *** functions of DMRT are mainly involved in sex development and the formation of many tissues and *** this study,a DM(Doublesex/Mab-3-domain gene was identified in the mud crab Scylla paramamosain,and was named SpDMRT99B because of its many similarities to arthropod DMRT99B and phylogenetically close relationship with arthropod *** cDNA of SpDMRT99B gene is 1249 bp in length,encoding 224 amino *** 254 bp to 928 bp there is a conserved DM *** transmembrane domain was *** multiple amino acid alignment and phylogenetic tree analysis,the closest gene to SpDMRT99B is Eriocheir sinensis DMRT99B,followed by Macrobrachium rosenbergii and Drosophila melanogaster *** expressions of the gene were characterized in different tissues of female and male crabs during early development period of crab individuals,as well as in different development periods of *** results showed that SpDMRT99B gene is significantly highly expressed in testis than in ovary and other *** expression level of SpDMRT99B in testis at different stages is significantly higher than that in ovary,and it is particularly highly expressed in immature *** early developmental stages of larvae,the expressions of SpDMRT99B remain at a low level and reach a peak at zoea stage I when the body segments shape *** is speculated that SpDMRT99B gene might be involved in the gonadal development process and somitogenesis of ***.

关键词： Scylla paramamosain DM domain gene SpDMRT99B sex determination/differentiation early development stage

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Antibacterial and antibiofi lm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bags

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Food Science and Human Wellness 2022年第6期11卷 1607-1613页

作者： Shen Yang Zijin Yuan Jude Juventus Aweya Shanggui Deng Wuyin Weng yueling zhang Guangming Liu College of Food and Biological Engineering Xiamen Key Laboratory of Marine Functional FoodJimei UniversityXiamen 361021China Department of Biology Guangdong Provincial Key Laboratory of Marine BiotechnologyShantou UniversityShantou 515063China College of Food and Pharmacy Zhejiang Ocean UniversityZhoushan 316000China

Bacteria contamination in Auricularia auricular culture bags reduces yield and increases the risk of food *** this study,5 species of bacteria,mainly gram-positive bacteria including three species of Bacillus spp.,Arthrobacter arilaitensis and Staphylococcus warneri,were isolated and identifi ed from bacteria-contaminated *** culture *** in silico predicted antimicrobial peptide from theβ-1,3-glucan-binding protein sequence of Penaeus vannamei,designated Pv GBP2(FLKLGRKSRYGMLKL),was screened and its antibacterial effect and mechanism of action on the isolated Bacillus *** minimal inhibitory concentrations(MIC)of Pv GBP2 on Bacillus *** 15.6–31.25μg/m *** Pv GBP2 could inhibit Bacillus subtilis in *** culture bags to maintain growth and yield of *** electron microscopy(TEM)revealed that Pv GBP2 kills bacteria by perforating the cell wall,destroying membrane integrity and resulting in the leakage of intracellular *** addition,Pv GBP2 inhibits biofi lm formation by *** by 90.6%at 1×***,peptide Pv GBP2 could be potentially applied as an antibacterial agent to control bacterial infection of *** cultivation and the spread of foodborne pathogens.

关键词： Auricularia auricular Antimicrobial peptide Penaeus vannamei β-1,3-Glucan-binding protein Bacillus spp.e

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Cholecystokinin octapeptide antagonizes apoptosis in human retinal pigment epithelial cells

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Neural Regeneration Research 2014年第14期9卷 1402-1408页

作者： Yuan Liu yueling zhang Zhaohui Gu Lina Hao Juan Du Qian Yang Suping Li Liying Wang Shilei Gong Department of Ophthalmology First Central Hospital of Baoding Department of Ophthalmology Hebei Province People’s Hospital Department of Endoscope Room First Central Hospital of Baoding

Although cholecystokinin octapeptide-8 is important for neurological function, its neuroprotective properties remain unclear. We speculated that cholecystokinin octapeptide-8 can protect human retinal pigment epithelial cells against oxidative injury. In this study, retinal pigment epithelial cells were treated with peroxynitrite to induce oxidative stress. Peroxynitrite triggered apoptosis in these cells, and increased the expression of Fas-associated death domain, Bax, caspase-8 and Bcl-2. These changes were suppressed by treatment with cholecystokinin octapeptide-8. These results suggest that cholecystokinin octapeptide-8 can protect human retinal pigment epithelial cells against apoptosis induced by peroxynitrite.

关键词： nerve regeneration retinal pigment epithelial cells peroxynitrite cholecystokininoctapeptide apoptosis Fas-associated death domain Bax Caspase-8 Bcl-2 neural regeneration

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Myo-inositol accelerates the metamorphosis from megalopa to crablet of Scylla paramamosain by modulating cuticle decomposition and reconstruction

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Aquaculture and Fisheries 2023年第6期8卷 695-705页

作者： Wenxiao Cui Hafiz Sohaib Ahmed Saqib Weifeng Gao Ruibo Wang Yang He Yang Yu Zhen Lin Qingyu zhang Yin zhang Shengkang Li Huaiping Zheng yueling zhang Mhd Ikhwanuddin Hongyu Ma Guangdong Provincial Key Laboratory of Marine Biotechnology Shantou UniversityShantou515063China STU-UMT Joint Shellfish Research Laboratory Shantou UniversityShantou515063China Rongtong Agricultural Development(Guangzhou)Co. LtdGuangzhou510030China Institute of Tropical Aquaculture and Fisheries Universiti Malaysia TerengganuKuala Nerus21030Malaysia

The mud crab Scylla paramamosain is a key species in China due to its high nutritional value and great economic worth and has grown in ***-inositol can modulate versatile physiological functions in aquatic *** the present study,*** megalopa were given graded concentrations of myo-inositol(0,1,2,4,and 8 ppm)by water immersion to explore how their metamorphosis would be *** results showed that supplementing with myo-inositol remarkably increased transformation and survival rate from megalopa to crablet by at least 1.16 and 1.26 times,*** decipher the molecular mechanism of how myo-inositol increases metamorphosis and survival rate,we further performed transcriptome-based gene expression profiling of both megalopa and crablet treated with *** integrative transcriptome analyses predicted that the differentially expressed genes(DEGs)were significantly enriched in chitinase activity,structural constituent of cuticle,and chitin binding,which are associated with the decomposition and reconstruction of *** results confirmed that myo-inositol mediated gene expression levels of the above cuticle-related *** the importance of the cuticle in exoskeleton formation and molting,it can be concluded that myo-inositol-induced changes in the cuticle decomposition and reconstruction might have accelerated the transformation from megalopa to crablet of ***,numerous DEGs were significantly enriched in protein digestion and absorption,amino sugar and nucleotide sugar *** implies that myo-inositol may improve survival by regulating energy or nutrient ***,the accelerated metamorphosis by myo-inositol may improve survival from megalopa to crablet of ***,this study will provide the first insights into the underlying molecular mechanisms by which myo-inositol increases metamorphosis and survival.

关键词： Myo-inositol Scylla paramamosain Cuticle decomposition and reconstruction Chitin Metamorphosis Survival

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外源硒对“夏黑”葡萄果实硒含量与品质的影响

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北方园艺 2025年第4期 68-75页

作者：王梅牛小沛马朝喜张月玲李铭刘星明济源市农业科学院河南济源459002

以五年生“夏黑”葡萄为试材,采用L_(9)(3^(4))正交实验方法,探讨外源硒喷施时期、剂量、频次等3个因素对果实硒含量与品质的影响,利用Topsis综合评价方法全面评估果实的品质,以期为富硒葡萄生产提供参考依据。结果表明:施用频次对“夏... 详细信息

以五年生“夏黑”葡萄为试材,采用L_(9)(3^(4))正交实验方法,探讨外源硒喷施时期、剂量、频次等3个因素对果实硒含量与品质的影响,利用Topsis综合评价方法全面评估果实的品质,以期为富硒葡萄生产提供参考依据。结果表明:施用频次对“夏黑”葡萄果实中硒含量影响最大,其次是剂量与时期,以A3B3C2组合(果实膨大期+600 mg·(667m^(2))^(-1)+2次)对葡萄果实硒富集效果最佳,果实硒含量高达0.1030 mg·kg^(-1)。叶面施硒既可以提高“夏黑”葡萄果实中的单穗质量、单果质量、果实纵径、果实横径、可溶性固形物、可溶性糖、维生素C含量,又能降低可滴定酸含量。根据Topsis综合评价方法分析出9个处理对改善果实品质大小顺序为T9>T3>T5>T7>T2>T8>T4>T6>T1。综合分析认为,果实膨大期叶喷施600 mg·(667m^(2))^(-1)硒肥,且喷施2次处理效果最佳。生产示范结果表明,该方案在不同种植区域应用均能生产出优质富硒葡萄。

关键词：外源硒葡萄果实硒含量品质

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我国海洋出水文物保护技术现状分析

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中国国家博物馆馆刊 2012年第6期 133-139页

作者：张月玲中国国家博物馆北京100006

随着我国水下考古事业近30年的发展,水下文物出水后的保护技术成为人们关注的新问题。为此,文物科技保护工作者做了大量工作并取得了一定成绩,但对于不断被发掘打捞出水的大量文物来说,出水文物科技保护人员寥寥可数,不能满足工作的需... 详细信息

随着我国水下考古事业近30年的发展,水下文物出水后的保护技术成为人们关注的新问题。为此,文物科技保护工作者做了大量工作并取得了一定成绩,但对于不断被发掘打捞出水的大量文物来说,出水文物科技保护人员寥寥可数,不能满足工作的需要。出水文物的科技保护面临新的任务和要求,需要有针对性地提出解决问题的方法和策略。

关键词：出水文物保护技术

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