Identification and Characterization of Gene SpDMRT99B and Its Sex-Biased Expression Profile in the Mud Crab,Scylla paramamosain

作     者：ZHANG Yin FANG Shaobin LIN Fei LI Shengkang ZHENG Huaiping ZHANG Yueling IKHWANUDDIN Mhd MA Hongyu ZHANG Yin;FANG Shaobin;LIN Fei;LI Shengkang;ZHENG Huaiping;ZHANG Yueling;IKHWANUDDIN Mhd;MA Hongyu

作者机构：Guangdong Provincial Key Laboratory of Marine BiotechnologyInstitute of Marine SciencesShantou UniversityShantou 515063China STU-UMT Joint Shellfish Research LaboratoryShantou UniversityShantou 515063China Institute of Tropical AquacultureUniversiti Malaysia TerengganuKuala Terengganu 21030Malaysia 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2021年第20卷第6期

页      面：1495-1504页

核心收录：

学科分类：0908[农学-水产] 0707[理学-海洋科学] 09[农学] 

基　　金：the National Natural Science Foundation of China(No.31772837) the National Key Research&Development Program of China(No.2018YF D0900201) the Science and Technology Project of Guang-dong Province(No.2018A050506080) the Shantou Uni-versity Scientific Research Foundation for Talents(No.NTF17006) the Program for Innovation and Enhance-ment of School of Department of Education of Guang-dong Province(No.2017KCXTD014) 

主　　题：Scylla paramamosain DM domain gene SpDMRT99B sex determination/differentiation early development stage 

摘      要：The doublesex and mab-3 related transcription factor(DMRT)gene family is conserved from invertebrates to *** functions of DMRT are mainly involved in sex development and the formation of many tissues and *** this study,a DM(Doublesex/Mab-3-domain gene was identified in the mud crab Scylla paramamosain,and was named SpDMRT99B because of its many similarities to arthropod DMRT99B and phylogenetically close relationship with arthropod *** cDNA of SpDMRT99B gene is 1249 bp in length,encoding 224 amino *** 254 bp to 928 bp there is a conserved DM *** transmembrane domain was *** multiple amino acid alignment and phylogenetic tree analysis,the closest gene to SpDMRT99B is Eriocheir sinensis DMRT99B,followed by Macrobrachium rosenbergii and Drosophila melanogaster *** expressions of the gene were characterized in different tissues of female and male crabs during early development period of crab individuals,as well as in different development periods of *** results showed that SpDMRT99B gene is significantly highly expressed in testis than in ovary and other *** expression level of SpDMRT99B in testis at different stages is significantly higher than that in ovary,and it is particularly highly expressed in immature *** early developmental stages of larvae,the expressions of SpDMRT99B remain at a low level and reach a peak at zoea stage I when the body segments shape *** is speculated that SpDMRT99B gene might be involved in the gonadal development process and somitogenesis of ***.