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Response of Heterotrophic Bacteria Abundance and Community Structure to Asian Dust Addition in the Oligotrophic Northwest Pacific Ocean

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Journal of Ocean University of China 2020年第3期19卷 722-728页

作者： CHEN Xi zhang xiaohao ZHAO Yangguo LIU Guangxing zhang Chao GAO Huiwang College of Environmental Science and Engineering Ocean University of ChinaQingdao 266100China Beijing Sound Environmental Engineering Co.Ltd. Beijing 101102China Key Lab of Marine Environmental Science and Ecology Ministry of EducationOcean University of ChinaQingdao 266100China

The dust storms from the continent usually affect the abundance and diversity of planktons by supplying trace elements. As such, the response of heterotrophic planktonic bacteria to dusts, nutrients(i.e., nitrogen and phosphorus) or ferrous dosages was investigated in the Kuroshio Extension region of the Northwest Pacific Ocean(NWPO) through on-board incubation experiments during an oceanographic survey in spring 2014. The flow cytometry and 16S rRNA high-throughput sequencing methods were applied to explore the abundance and community structure of bacteria, and the percentage of high nucleic acid bacteria(HNA%). The results showed that the heterotrophic bacteria abundance was low(average 2.55×10^5 cells mL^-1) and subjected to both nitrogen(N) and ferrous(Fe) limitation. Sand-dust deposition observably promoted the activity of heterotrophic planktonic bacteria. The maximum abundance of heterotrophic bacteria was 6.98×10^5 cells mL^-1 in the dust-dosage group, which was 44% higher than the control(P < 0.05). The HNA% in the dust-dosage group was 1.37 times higher than the control(P < 0.05). The activation mechanism was mainly related to the dissolution of N and Fe in the dusts. The relative abundance of genus Winogradskyella was significantly increased by dust deposition while the relative abundance of the genera Tenacibaculum and Hyphomonas was decreased. These variations of bacterial community structure were ascribed to the dissolution of nutrients N and P. Comparing the results of different experimental groups, this study concluded that dust storm improved the abundance of heterotrophic bacteria by dissolution of N and Fe.

关键词： Northwest Pacific Asian dust heterotrophic bacteria 16S rRNA high-throughput sequencing bacteria abundance and community structure

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An Automatic Implementation of Oropharyngeal Swab Sampling for Diagnosing Respiratory Infectious Diseases via Soft Robotic End-Effectors

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Chinese Journal of Mechanical Engineering 2024年第2期37卷 55-67页

作者： Yafeng Cui Wenjie Yu Jingjing Li Qi Shao Ding Weng Guoping Yin xiaohao zhang Xinjun Liu Jingying Ye Jiadao Wang Huichan Zhao Department of Mechanical Engineering Tsinghua UniversityBeijing 100084China Beijing Huakang Tongbang Technology Company Beijing 102609China Department of Otorhinolaryngology Beijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijing 102218China Tianjin Research Institute for Advanced Equipment Tsinghua UniversityTianjin 300300China

The most widely adopted method for diagnosing respiratory infectious diseases is to conduct polymerase chain reaction(PCR)assays on patients’respiratory specimens,which are collected through either nasal or oropharyngeal *** manual swab sampling process poses a high risk to the examiner and may cause false-negative results owing to improper *** this paper,we propose a pneumatically actuated soft end-effector specifically designed to achieve all of the tasks involved in swab *** soft end-effector utilizes circumferential instability to ensure grasping stability,and exhibits several key properties,including high load-to-weight ratio,error tolerance,and variable swab-tip stiffness,leading to successful automatic robotic oropharyngeal swab sampling,from loosening and tightening the transport medium tube cap,holding the swab,and conducting sampling,to snapping off the swab tail and sterilizing *** an industrial collaborative robotic arm,we integrated the soft end-effector,force sensor,camera,lights,and remote-control stick,and developed a robotic oropharyngeal swab sampling *** this swab sampling system,we conducted oropharyngeal swab-sampling tests on 20 *** Digital PCR assay results(RNase P RNA gene absolute copy numbers for the samples)revealed that our system successfully collected sufficient numbers of cells from the pharyngeal wall for respiratory disease *** summary,we have developed a pharyngeal swab-sampling system based on an“enveloping”soft actuator,studied the sampling process,and imple-mented whole-process robotic oropharyngeal swab-sampling.

关键词： Diagnosis Medical robot Soft end-effector Swab-sampling Digital PCR

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Off-hour effect is not significant in endovascular treatment for anterior circulation large vessel occlusion in a multicentre registry

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Stroke & Vascular Neurology 2021年第4期6卷 640-648,I0093页

作者： Mingming Zha Qingwen Yang Shuo Liu Kangmo Huang xiaohao zhang Min Wu Haodi Cai Qiushi Lv Rui Liu Dong Yang Xinfeng Liu Department of Neurology Southeast UniversityNanjingJiangsuChina Department of Internal Medicine Southeast UniversityNanjingJiangsuChina Department of Neurology Medical School of Nanjing UniversityNanjingJiangsuChina Department of Neurology Southern Medical UniversityGuangzhouGuangdongChina Department of Neurology Nanjing Jinling HospitalNanjingJiangsuChina

Background and purpose Whether the off-hour effect has an impact on workflow and outcomes of endovascular treatment(EVT)for anterior circulation large vessel occlusion(AC-LVO)remains *** study aimed to compare the characteristics and outcomes of patients who presented or were treated during off-hour versus on-hour in a multi-center *** AC-LVO patients from 21 centres were categorised into the off-hour group and the on-hour ***-hour(weekends,holidays,and 18:00-7:59 on weekdays)and on-hour(8:00-17:59 on weekdays except for holidays)were defined according to arrival and groin-puncture time points,*** comparisons between patients both arrived and treated during off-hour(true off-hour)and on-hour(true on-hour)were *** primary outcome was the 90-day modified Rankin Scale(mRS)*** outcomes included favourable outcome(mRS 0-2 at 90 days),EVT-related time metrics,and other clinical *** and binary logistic regression and linear regression were taken to adjust for confounding *** Of all 698 patients enrolled,435(62.3%)and 456(65.3%)patients were categorised into the off-hour arrival and off-hour puncture group,*** onset to door time(adjustedßcoefficient:−21.56;95%CI−39.96 to−3.16;p=0.022)was noted in the off-hour arrival *** and dichotomous mRS scores at 90 days were comparable between the off-hour group and the on-hour group regardless of off-hour *** time metrics and outcomes were comparable between the two *** 595 patients both presented and were treated during off-hour or on-hour,394 patients were categorised into the true off-hour group and 201 into the true on-hour *** metrics and clinical outcomes were similar between the true off-hour and the true on-hour *** The off-hour effect was not significant regarding clinical outcomes and in-hospital workflow in AC-LVO patients receiving EVT in this Chinese multicentr

关键词： centre treatment effect

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Clinical outcomes of asymptomatic low-grade esophagitis:results from a multicenter Chinese cohort

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Gastroenterology Report 2022年第1期10卷 507-512页

作者： Songfeng Chen Xuelian Xiang xiaohao zhang Qianjun Zhuang Niandi Tan Xun Hou Mengyu zhang Junnan Hu Chaofan Duan Yi Cui Jinhui Wang Xiangbin Xing Nina zhang Yinglian Xiao Department of Gastroenterology The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdongP.R.China Department of Gastroenterology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiP.R.China Gastrointestinal Surgery Center The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdongP.R.China Department of Gastroenterology The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjingJiangsuP.R.China

Background:Asymptomatic low-grade(Los Angeles Classification Grades A and B)esophagitis is common in clinical practice with unclear clinical *** study aimed to explore the clinical outcomes of asymptomatic low-grade ***:This was a multicenter cohort study conducted by three academic hospitals in *** low-grade esophagitis patients between January 2015 and December 2019 were *** healing condition 1year after initial diagnosis,symptomoutcomes,and proton-pump inhibitor(PPI)use within 1year after initial diagnosis were studied and ***:A total of 248 asymptomatic low-grade esophagitis patients were *** disappeared in 76.2%of patients 1 year after initial *** terms of symptom outcomes,89.9%of patients did not present gastroesophageal reflux disease(GERD)symptoms within 1 year after initial *** significant difference was found in the proportion of patients who presented GERD symptoms and in the proportion of patients with persistent esophagitis between the PPI group and the non-PPI group(all P>0.05).Patients with initial Grade B esophagitis were more likely to present follow-up GERD symptoms(16.0%vs 7.5%,P=0.041)and had more severe follow-up esophagitis than those with Grade A(P<0.001).Patients with follow-up GERD symptoms were more likely to have persistent esophagitis than those ***:This study demonstrated that asymptomatic low-grade esophagitis had relatively benign clinical *** with initial Grade B esophagitis and patients with follow-up GERD symptoms were more likely to be those who are in genuine need of further follow-up and treatments.

关键词： esophagitis asymptomatic proton-pump inhibitor symptom mucosal

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Study on simulation and experiment of array micro Faraday cup ion detector for FAIMS

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Science China(Technological Sciences) 2010年第12期53卷 3225-3231页

作者： TANG Fei, WANG xiaohao, zhang Liang & YAN ZiLin State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments and Mechanology, Tsinghua University, Beijing 100084, China State Key Laboratory of Precision Measurement Technology and Instruments Department of Precision Instruments and Mechanology Tsinghua University Beijing China

An array micro Faraday cup ion detector for FAIMS (High-field Asymmetric Waveform Ion Mobility Spectrometry) was introduced, with which the size of the FAIMS system was reduced. With simple structure, good stability, low noise, large measurements range, this detector can work under the condition of atmospheric pressure. The array micro Faraday cup was composed of gate electrode, sensitive electrode and shielding electrode. The sensitive electrode was made of tens of crossing silicon columns with diameter of 200 μm. It was fabricated through typical MEMS technology, which was compatible completely with plane FAIMS. It was shown from FLUENT simulation result that the resistance to gas motion was low and the flow field distribution was helpful for full absorption of the ion in this array design. Through electricity simulation, the method to reduce interference on the detection signal of the micro Faraday cup was given out. Connecting with KEITHLEY 237 ampere meter, the noise level of the array micro Faraday cup was lower than 0.5 pA. The output signal of the acetone sample measured by experiment was about 210 pA. Through contrast experiment, the design parameter of the micro Faraday cup was obtained primarily. This array micro Faraday cup can meet the requirements of the FAIMS system.

关键词： FAIMS MEMS Faraday cup ion detector low current

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委托—代理理论视角的民营上市公司控制权博弈

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武汉大学学报（哲学社会科学版） 2006年第3期59卷 348-354页

作者：张晓昊武汉大学经济与管理学院湖北武汉430072

企业作为一系列投入要素的契约集合,其契约的核心是对企业剩余的配置。企业剩余配置机制是公司激励约束机制的核心,股东和管理者的激励约束机制是一种双向的动态的重复博弈过程。关于公司控制权的争夺本质上也是为了通过创造更大的信息... 详细信息

企业作为一系列投入要素的契约集合,其契约的核心是对企业剩余的配置。企业剩余配置机制是公司激励约束机制的核心,股东和管理者的激励约束机制是一种双向的动态的重复博弈过程。关于公司控制权的争夺本质上也是为了通过创造更大的信息不对称度来获得更大的企业剩余控制权,对公司控制权的争夺过程就是这种动态重复博弈的表现。但是, 中国民营上市公司实际上处于一种古典类型向现代企业的初级转型阶段,公司的股权虽然分散,但股东通过各种方式强化了对公司的控制权,管理者与股东争夺控制权的博弈并不明显, 导致管理层与股东形成“联合企业家”、通过“侵占效应”、“共谋”侵占。

关键词：企业剩余委托—代理理论控制权民营上市公司

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一星即差评?评论效价对酒店评论有用性的影响:一项混合方法研究

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旅游科学 2023年第2期37卷 77-99页

作者：鄢慧丽张小浩熊浩海南大学旅游学院海南海口570228 海南大学管理学院海南海口570228

现有关于评论效价对评论有用性影响的研究结论并不一致,且在关键变量的测量上存在局限性。文章基于前景理论,构建一个有调节的中介模型,并采用更为严谨的大数据分析与情景实验的混合方法,探讨评论效价(负面评论vs.正面评论)对酒店评论... 详细信息

现有关于评论效价对评论有用性影响的研究结论并不一致,且在关键变量的测量上存在局限性。文章基于前景理论,构建一个有调节的中介模型,并采用更为严谨的大数据分析与情景实验的混合方法,探讨评论效价(负面评论vs.正面评论)对酒店评论有用性影响关系的作用机制及边界条件。结果表明:(1)评论效价对评论有用性存在显著的负向影响,即相较于正面评论,负面评论有用性更高;(2)感知风险在评论效价与评论有用性之间起部分中介作用;(3)情感强度在评论效价对评论有用性直接影响中起调节作用,即对于负面评论,情感强度越高,评论有用性越高,但这种影响差异对正面评论不显著;(4)情感强度对评论效价与评论有用性的中介路径也存在调节作用,具体而言,负面评论的情感强度越高,消费者对产品的感知风险越强,评论有用性也就越强;而正面评论的情感强度高低并不会引起感知风险的显著变化,因而对评论有用性的影响差异也不明显。文章有助于揭示评论效价对评论有用性影响的作用机制,也对酒店和平台的在线评论管理和营销实践具有一定的启示意义。

关键词：评论效价评论有用性感知风险情感强度前景理论

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酒店评论中感官线索对评论有用性的影响——基于心理模拟视角的实证分析

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旅游学刊 2023年第2期38卷 120-133页

作者：鄢慧丽熊浩张小浩海南大学旅游学院海南海口570228 海南大学管理学院海南海口570228

感官是消费者接触和获取产品信息的基础方式。近年来,企业营销战略也逐渐向“感官”转变,因此,对酒店评论中感官线索与评论有用性的关系研究值得关注。文章基于心理模拟视角,以携程网130家五星级酒店的69752条评论为样本,构建酒店领域... 详细信息

感官是消费者接触和获取产品信息的基础方式。近年来,企业营销战略也逐渐向“感官”转变,因此,对酒店评论中感官线索与评论有用性的关系研究值得关注。文章基于心理模拟视角,以携程网130家五星级酒店的69752条评论为样本,构建酒店领域感官情感词典,实现了酒店评论中感官线索的自动识别与情感计算,进而利用负二项回归计量模型,探讨感官线索的数量、维度以及情感等特征对酒店评论有用性的影响。研究结果显示:(1)感官线索数量对酒店评论有用性存在显著的积极影响,且不同感官线索对评论有用性的影响存在差异,其影响程度依次为嗅觉、视觉、味觉和触觉,而听觉线索对评论有用性的影响并不显著;(2)不同感官线索之间存在整合效应,即相较于单一维度感官线索,多感官线索对酒店评论有用性的影响更强,且感官维度叠加对评论有用性的影响比感官数量叠加对评论有用性的影响更强;(3)相较于负面感官线索,正面感官线索对酒店评论有用性的影响更强。该研究为感官体验研究提供了有益补充,同时,对酒店和平台的在线评论管理与感官营销实践具有重要的参考价值。

关键词：感官数量多感官整合感官情感评论有用性心理模拟

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基于需求跨时流转的景区门票折扣优化模型

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华中师范大学学报（自然科学版） 2022年第1期56卷 140-145,179页

作者：张小浩鄢慧丽熊浩海南大学旅游学院海口570228 海南大学管理学院海口570228

当景区存在明显的淡旺季门票销售差异时,一般会选择在淡季通过门票折扣进行促销.但是,如果淡季门票折扣促销对景区旺季需求产生影响时,就需要进行整体收益管理,而不是单独的淡季收益管理.该研究提出了一种新的研究视角进行整体收益管理... 详细信息

当景区存在明显的淡旺季门票销售差异时,一般会选择在淡季通过门票折扣进行促销.但是,如果淡季门票折扣促销对景区旺季需求产生影响时,就需要进行整体收益管理,而不是单独的淡季收益管理.该研究提出了一种新的研究视角进行整体收益管理:基于游客价格敏感程度差异化及其构成比例,结合潜在游客需求从旺季向淡季流转的特性,进行折扣优化模型构建,给出了迭代求解方法,并进行了实例演算.实例结果计算显示:1)考虑流转时,淡季的折扣会更大,并且流转系数越高折扣就会越多;2)敏感型顾客占比越高折扣会越多.从实证结果看,折扣优化决策模型符合景区淡旺季门票折扣决策的实践,可以为具有明显淡旺季的景区门票定价决策提供有益参考.

关键词：景区门票价格折扣优化模型需求跨时流转淡旺季收益管理

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论警察心理危机干预中社会支持系统的构建

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浙江警察学院学报 2011年第1期23卷 46-49页

作者：张小浩中国人民公安大学北京100038

长期从事"三高"工作,警察面临突发的创伤性事件持续增多,造成的心理压力不断加大,若超出其心理承受能力,往往容易产生心理危机。在警察心理危机干预过程中,社会支持系统的建立和完善对个体心理健康的恢复具有重要意义。警察社会支持系... 详细信息

长期从事"三高"工作,警察面临突发的创伤性事件持续增多,造成的心理压力不断加大,若超出其心理承受能力,往往容易产生心理危机。在警察心理危机干预过程中,社会支持系统的建立和完善对个体心理健康的恢复具有重要意义。警察社会支持系统包括组织支持、家庭支持、朋辈支持以及社会支持,在危机干预中应该把构建警察良好的社会支持系统作为重要内容。

关键词：警察心理心理危机危机干预社会支持系统

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