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Prevalence and characteristics of extended spectrum β-lactamase-producing Escherichia coli from bovine mastitis cases in China

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Journal of Integrative Agriculture 2018年第6期17卷 1246-1251页

作者： YANG Feng zhang shi-dong SHANG Xiao-fei WANG Xu-rong WANG Ling YAN Zuo-ting LI Hong-sheng Lanzhou Institute of Husbandry and Pharmaceutical Sciences Chinese Academy of Agricultural Sciences

The aim of the study was to investigate the prevalence and characterization of extended-spectrum β-lactamase （ESBL）- producing Escherichia coli isolated from bovine mastitis cases in China. ChromID ESBL agar was used to confirm ESBL-producing E. coli. PCR and DNA sequencing were employed to characterize the genotype of ESBL-producers. Antimicrobial susceptibility was measured by disc diffusion. Overall, 73 of 318 E. coli isolates （22.96％） were identified as ESBL-producers. Of these ESBL-producing E. coli, the prevalence of blaCTX-M and blaTEM-1 was 97.26 and 71.23％, respectively. The predominant CTX-M-type ESBL was CTX-M-15 （65.75％）, followed by CTX-M-14 （10.96％）, CTX-M-55 （9.59％）, CTX-M-64 （5.48％）, CTX-M-65 （4.11％） and CTX-M-3 （1.37％）. This study is the first report of CTX-M-64 and CTX-M-65 in E. coli isolated from bovine mastitis. Furthermore, 72 ESBL-producing E. coli isolates （98.63％） were found to be multidrug-resistance. This study noted high prevalence and rates of antimicrobial resistance of ESBL-producing E. coli isolates from bovine mastitis cases in China.

关键词： extended spectrum β-lactamase Escherichia coli multidrug-resistance bovine mastitis

Genetic characterization of antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis cases in Northwest China

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Journal of Integrative Agriculture 2016年第12期15卷 2842-2847页

作者： YANG Feng WANG Qi WANG Xu-rong WANG Ling LI Xin-pu LUO Jin-yin zhang shi-dong LI Hong-sheng Key Laboratory of Veterinary Pharmaceutical Development Ministry of Agriculture/Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province/Lanzhou Institute of Husbandry and Pharmaceutical SciencesChinese Academy of Agricultural Sciences State Key Laboratory of Applied Organic Chemistry Lanzhou University

Staphylococcus aureus is the most common etiological pathogen of bovine mastitis. The resistant strains make the disease difficult to cure. The aim of this study was to characterize the genetic nature of the antimicrobial resistance in S. aureus cultured from bovine mastitis in Northwest China in 2014. A total of 44 S. aureus were isolated for antimicrobial resistance and resistance-related genes. Antimicrobial resistance was determined by disc diffusion and the corresponding resistance genes were detected by PCR. Phenotype indicated that S. aureus isolates were resistant to penicillin （84.09%）, erythromycin （20.45%）, tetracycline （15.91%）, gentamicin （9.09%）, tobramycin （6.82%）, kanamycin （6.82%） and methicillin （2.27%）. 9.09% of the S. aureus isolates were classified as multidrug resistant. In addition, genotypes showed that the isolates were resistant to rifampicin （100%, rpoB）, penicillin （95.45%, blaZ）, tetracycline （22.73%, tetK, tetM, alone or in combination）, erythromycin （22.73%, ermB or ermC）, gentamicin/tobramycin/kanamycin （2.27%, aacA-aphD）, methicillin （2.27%, mecA） and vancomycin （2.27%, vanA）. Resistance to tetracycline was attributed to the genes tetK and tetM （r=0.558, P〈0.001）. This study noted high-level geno- and phenotypic antimicrobial resistance in S. aureus isolates from bovine mastitis cases in Northwest China.

关键词： bovine mastitis Staphylococcus aureus antimicrobial resistance phenotype genotype

Performance of the bio-inspired leading edge protuberances on a static wing and a pitching wing

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Journal of Hydrodynamics 2014年第6期26卷 912-920页

作者：王雅赟胡文蓉张仕栋 Department of Engineering Mechanics Shanghai Jiao Tong University MOE Key Laboratory of Hydrodynamics Shanghai Jiao Tong University Shanghai Jiao Tong University and Chiba University International Cooperative Research Center (SJTC-CU- ICRC) Shanghai Jiao Tong University Shanghai Jiao Tong University and Chiba University International Cooperative Research Center (SJTC-CU-ICRC) Shanghai Jiao Tong University

It is shown that the leading edge protuberances on the flippers of a humpback whale can significantly improve the hydrodynamic performance. The present study numerically investigates the flow control mechanisms of the leading edge protuberances on a static wing and a pitching wing. For static wings, the performance in both laminar flow and turbulent flow are studied in the context of the flow control mechanisms. It is shown that the protuberances have slight effects on the performance of static wings in laminar flow. Also, it could be deduced that non-uniform downwash does not delay the stall occurrence in either laminar flow or turbulent flow. In turbulent flow, the leading edge protuberances act in a manner similar to vortex generators, enhancing the momentum exchange within the boundary layer. Streamwise vortices do contribute to the delay of the stall occurrence. The normal vorticity component also plays an important role in delaying the stall occurrence. However, for the pitching wing, the effect of leading edge protuberances is negligible in turbulent flow. Detailed analysis of the flow field indicates that for the wing with the leading edge protuberances, the leading edge vortices become more complex, while the thrust jet and the vortices in the wake are not changed significantly by the leading edge protuberances.

关键词： leading edge protuberance turbulent flow laminar flow a pitching wing a static wing

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“夫余”与“句丽”语义考释

东北师大学报（哲学社会科学版） 2010年第6期 154-157页

作者：张士东东北师范大学外国语学院吉林长春130024

夫余一词最初是指舒缓起伏的平原和丘陵。作为族名,意思是生活在那片舒缓起伏的平原和丘陵地区的人们。句丽一词最初指弯曲隆起的高山。作为族名,其与"离"一样,都是指生活在弯曲隆起的山地的人们。夫余、离、句丽,作为族名,都源自其所... 详细信息

夫余一词最初是指舒缓起伏的平原和丘陵。作为族名,意思是生活在那片舒缓起伏的平原和丘陵地区的人们。句丽一词最初指弯曲隆起的高山。作为族名,其与"离"一样,都是指生活在弯曲隆起的山地的人们。夫余、离、句丽,作为族名,都源自其所活动区域的地貌特征。

关键词：夫余句丽方言历史语言学古语

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“濊”、“粟”语义考释

东北师大学报（哲学社会科学版） 2012年第3期 130-133页

作者：张士东东北师范大学外国语学院吉林长春130024

"濊"字的本义是"大水",濊族所在的位置是中国东北地区的第二松花江流域、鸭绿江流域,以及朝鲜半岛的江原道和咸镜南、北道。"濊"作为族称,含义是生活在水边的民族。"粟"字的本义也是"水",粟末族所在的位置是第二松花江流域。"粟"作为族... 详细信息

"濊"字的本义是"大水",濊族所在的位置是中国东北地区的第二松花江流域、鸭绿江流域,以及朝鲜半岛的江原道和咸镜南、北道。"濊"作为族称,含义是生活在水边的民族。"粟"字的本义也是"水",粟末族所在的位置是第二松花江流域。"粟"作为族称,含义也是生活在水边的民族。喉牙音"濊"曾经发生了到舌尖前音"粟"的历史音变。

关键词：濊濊貊粟粟末历史音变

AKT1 and AKT2 Promote Malignant Transformation in Human Brain Glioma LN229 Cells

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Clinical oncology and cancer researeh 2011年第3期8卷 144-148页

作者： Jian ZOU Kun WANG Lei HAN An-ling zhang Zhen-dong shi Pei-yu PU Chun-sheng KANG Department of Neurosurgery Tianjin Medical University General Hospital Laboratory of Neuro-oncology Tianjin Neurological Institute Tianjin 300052 China

OBJECTIVE To confirm the role played by AKT1 and AKT2 in the β- catenin/Tcf-4 signaling pathway in promoting malignant transfor- mation of glioma cells. METHODS LN229 cells were divided into five groups： a control group, acetone （ACE）group, acetylsalicylic acid （ASA; aspirin） group, ASA＋AKT1 plasmid group and ASA＋AKT2 plasmid group. Western blot and PCR were used to detect the expression of AKT1 and AKT2 after dealing with ASA and transferring AKTI/2 genes into LN229 cells. Cell proliferation was determined by flow cytometry, cell invasion was evaluated by transwell assay and cell apoptosis was detected with annexin V staining. The molecules regulating proliferation and invasion were examined by western blot analysis. RESULTS Aspirin down-regulates AKT1 and AKT2 expression by modulating β-cateninfrcf-4 activity. AKT1 and AKT2 can enhance cell proliferation and invasion by up-regulating the expression of cyclin-D and matrix metalloprotein-9 （MMP-9） in LN229 glioma cells. CONCLUSION AKT1 and AKT2 play an important role in the β- catenin/Tcf-4 signaling pathway promoting malignant transformation; AKT1 is more effective than AKT2. AKT1 and AKT2 may be potential targets for brain glioma therapy and an effective way to prevent metastasis of gliomas.

关键词： AKT1 AKT2 brain glioma malignant transformation.

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中国翻译产业发展态势及对策研究

东北师大学报（哲学社会科学版） 2016年第1期 48-52页

作者：张士东彭爽东北师范大学外国语学院吉林长春130024 东北师范大学文学院吉林长春130024

互联网时代是一个百业争先和合作共赢的时代,为翻译产业创造了许多契机,同时也对翻译产业提出了新的挑战。翻译产业属于语言产业,它通过提供两种或多种语言的转译活动,以满足人们对他种语言为载体的信息需求的一种生产经营活动。借助计... 详细信息

互联网时代是一个百业争先和合作共赢的时代,为翻译产业创造了许多契机,同时也对翻译产业提出了新的挑战。翻译产业属于语言产业,它通过提供两种或多种语言的转译活动,以满足人们对他种语言为载体的信息需求的一种生产经营活动。借助计算机和"互联网",翻译产业从传统服务业发展到现代新兴产业。翻译产业优势充分,劣势明显,机会较多,威胁较大。基于此,必须采取相应对策:在战略上,提高对翻译产业重要性的认识,加强人才培养和产品开发;在经营上,实现产业的四个结合,即古今、产学研、产业链、国内外;在管理上,由政府进行制度把关,将翻译产业落到实处。

关键词： “互联网+” 语言产业翻译产业 swot分析法对策

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表层任意性和深层理据性的辩证

东北师大学报（哲学社会科学版） 2014年第2期 112-115页

作者：张士东彭爽东北师范大学外国语学院吉林长春130024 东北师范大学文学院吉林长春130024

约定俗成表面上看是任意性,拟声词、叹词也因语言的不同而被看成是相对任意的。但是经过逻辑分析以及从汉语的具体实例来看,任意性都是表面现象,其深层藏着很深的理据,也就是声音的历史演变规律和语义的引申变化。在这种证据确凿的考据... 详细信息

约定俗成表面上看是任意性,拟声词、叹词也因语言的不同而被看成是相对任意的。但是经过逻辑分析以及从汉语的具体实例来看,任意性都是表面现象,其深层藏着很深的理据,也就是声音的历史演变规律和语义的引申变化。在这种证据确凿的考据面前,我们认为理据性是恰当的。但是有时这种深层的理据隐藏得太深,以至于我们在没有获得确切的答案时,姑且认为其关系是任意的。

关键词：任意性理据性语音语义能指所指

Effect of Chanfukang(Chinese Medicine)on Endothelin and Nitric Oxide in Postpartum Cows with Qi Deficiency and Blood Stasis

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Animal Husbandry and Feed Science 2010年第3期2卷 39-42页

作者： YAN Zuo-ting WANG dong-sheng YANG Guo- lin XIE Jia-sheng LI shi-hong zhang shi-dong GONG Zhong-fu Lanzhou Institute of Animal Husbandry and Veterinary Medicine Chinese Academy of Agricultural Sciences Lanzhou 337000 China China Institute of Veterinary Drug Control Beijing 100081 China

[ Objective] To observe effect of Chanfukang on vascular endothelial cells of postpartum cows with Oi deficiency and Blood stasis, and to explore the treatment mechanism of Chanfukang. [Method] A total of 58 cows were assigned to four groups. The cows in group 1 and group 2 suffered from Qi deficiency and Blood stasis. The cows in group 1 were gavaged with Chanfukang （0.5 g/kg-BW, 5 d） at 12 h post partum, but the cows in group 2 were not treated. The cows in group 3 were healthy and gavaged with Chanfukang （0.4 g/***, 5 d） at 12 h post partum. Cows in group 4 were healthy control cows. The plasma endothelin （ET） concentration and serum nitric oxide （NO） concentration were determined before and after parturition, respectively. [ Result ] The plasma ET concentration and serum NO concentration were significantly higher in the cows with Qi deficiency and Blood stasis than in the healthy control cows （P 〈 0.05）. The NO concentration decreased on Day 10 post partum in the cows with Qi deficiency and Blood stasis, but it was still higher than that in the healthy control cows. After taking Chanfukang for 7 d, the ET concentration and NO concentration decreased, and no significant difference was found between the cows with Qi deficiency and Blood stasis treated with Chanfukang and the healthy control cows. [ Conclusion] Chanfukang may relieve Qi deficiency and Blood stasis of postpartum cows through decreasing plasma ET concentration and serum NO concentration.

关键词： Chanfukang Qi deficiency and Blood stasis Dairy cows Endothelin Nitric oxide