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Blown sand motion within the sand-control system in the southern section of the Taklimakan Desert Highway

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Journal of Arid Land 2015年第5期7卷 599-611页

作者： CHENG Hong HE Jiajia XU Xingri ZOU Xueyong WU Yongqiu LIU Chenchen DONG Yifan PAN meihui WANG Yanzai zhang Hongyan State Key Laboratory of Earth Surface Processes and Resource Ecology Beijing Normal University MOE Engineering Center of Desertification and Blown-sand Control at Beijing Normal University Lushunkou Trading Center of Land Reserve

Although scientists have performed many studies in the Taklimakan Desert, few of them have reported the blown sand motion along the southern edge of the Taklimakan Desert Highway, which differs significantly from the northern region in terms of aeolian sand geomorphology and formation environment. Based on the field ob- servation data of airflow and aeolian sand transport, continuous monitoring data of erosional and depositional processes between 14 April 2009 and 9 April 2011 and data of surface sand grains from the classical section along the southern edge of the Taklimakan Desert Highway, this paper reported the blown sand motion within the sand-control system of the highway. The main results are as follows： 1） The existing sand-control system is highly effective in preventing and controlling desertification. Wind velocities within the sand-control system were ap- proximately 33%-100% of those for the same height above the mobile sand surface. Aeolian sand fluxes were approximately 0-31.21% of those of the mobile sand surface. Sand grains inside the system, with a mean diameter of 2.89 q）, were finer than those （2.15 q）） outside the system. In addition, wind velocities basically followed a loga- rithmic law, but the airflow along the classical section was mainly determined by topography and vegetation. 2） There were obvious erosional and depositional phenomena above the surface within the sand-control system, and these phenomena have very consistent patterns for all observation points in the two observed years. The total thicknesses of erosion and deposition ranged from 0.30 to 14.60 cm, with a mean value of 3.67 cm. In contrast, the deposition thicknesses were 1.90-22.10 cm, with a mean value of 7.59 cm, and the erosion thicknesses were 3.51-15.10 cm, with a mean value of 8.75 cm. The results will aid our understanding of blown sand within the sand-control system and provide a strong foundation for optimizing the sand-control system.

关键词： sand-control system blown sand motion erosion deposition Taklimakan Desert Highway

A micro-nano optogenetic system based on probiotics for in situ host metabolism regulation

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Nano Research 2023年第2期16卷 2829-2839页

作者： Xinyu zhang Ning Ma Wei Ling Gaoju Pang Tao Sun Jing Liu Huizhuo Pan meihui Cui Chunli Han Chun Yang Jin Chang Xian Huang Hanjie Wang School of Life Sciences Tianjin UniversityTianjin 300072China School of Precision Instrument and Optoelectronics Engineering Tianjin UniversityTianjin 300072China School of Chemical Engineering&Technology Tianjin UniversityTianjin 300072China

Genetically engineered bacteria have aroused attention as micro-nano drug delivery systems in ***,conventional designs of engineered bacteria usually function constantly or autonomously,which might be non-specific or ***,designing and optimizing in situ control strategy are important methodological progress for therapeutic researches of intestinal engineered ***,a micro-nano optogenetic system based on probiotic was developed combining microelectronics,nanotechnology,and synthetic biology to achieve in situ controllable drug ***,optogenetic engineered Lactococcus lactis was orally administrated in the intestinal tract.A wearable optical device was designed to control optical signals ***,*** could be customized to secrete peptides according to optical *** an example,optogenetic *** system can be constructed to secrete glucagon-like peptide-1(GLP-1)under the control of the wearable optical device to regulate *** improve the half-life of GLP-1 in vivo,Fc-domain fused GLP-1 was optimally *** this strategy,blood glucose,weight,and other features were well controlled in rats and mice ***,upconversion microcapsules were introduced to increase the excitation wavelength of the optogenetic system for better *** strategy has biomedical potential to expand the toolbox for intestinal engineered bacteria.

关键词： synthetic biology optogenetics upconversion nanoparticles microelectronics diabetics

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长林小蠹对黑松挥发物电生理及行为反应

森林工程 2024年第3期40卷 101-114,124页

作者：许建娇张瀚文陈焕文周俊华王美惠石鹏杨青山项嘉建宇佳东北林业大学林学院哈尔滨150040

为探究长林小蠹对不同生理状态黑松(Pinus thunbergii)挥发物的选择偏好性,利用动态顶空吸附法,分别收集健康和不同受害程度的黑松树干和树根的挥发物,通过气相色谱-质谱联用仪(Gas chromatography-mass spectrometry,GC-MS)对收集的挥... 详细信息

为探究长林小蠹对不同生理状态黑松(Pinus thunbergii)挥发物的选择偏好性,利用动态顶空吸附法,分别收集健康和不同受害程度的黑松树干和树根的挥发物,通过气相色谱-质谱联用仪(Gas chromatography-mass spectrometry,GC-MS)对收集的挥发物进行鉴定与分析,采用触角电位仪(Electroantennagraphy,EAG)和Y形嗅觉仪,分析鉴定到的黑松中主要挥发物及其他人鉴定到的黑松中主要挥发物对长林小蠹的电生理和行为影响。结果表明,健康和不同受害程度的黑松树干、树根的挥发物在组成和含量上部分差异显著(P<0.05)。树干中主要化合物(1R)-(+)-α-蒎烯的相对含量由健康木转向萎蔫木阶段呈下降趋势,在萎蔫木到枯死木阶段相对含量上升。而树根由健康木阶段转向濒死木阶段时(1R)-(+)-α-蒎烯的相对含量逐渐减少,在枯死木阶段其相对含量有所上升。EAG测试结果显示,在测定的10种挥发物中,有9种引发的雌成虫和雄成虫EAG反应值之间有显著差异。其中,雌虫对0.1 mg/mL的(1S)-(-)-α-蒎烯的反应值最高,雄虫对0.01 mg/mL的罗勒烯反应值最高。Y形嗅觉仪行为反应结果显示,不同质量浓度的挥发物对长林小蠹雌雄虫的作用不同。0.01~100 mg/mL的α-蒎烯、100 mg/mL的α-松油烯、10 mg/mL和100 mg/mL的(1S)-(-)-α-蒎烯、(1R)-(+)-α-蒎烯对长林小蠹雌雄成虫都有较强的吸引作用,10 mg/mL的(-)-β-蒎烯对长林小蠹雌雄成虫都有较强的驱避作用。由此,0.01~100 mg/mL的α-蒎烯;100 mg/mL的α-松油烯、10 mg/mL和100 mg/mL的(1S)-(-)-α-蒎烯、(1R)-(+)-α-蒎烯可以作为长林小蠹雌雄成虫引诱剂成分,10 mg/mL的(-)-β-蒎烯可作为长林小蠹成虫驱避剂成分。

关键词：长林小蠹挥发性化合物 GC-MS 触角电位行为反应

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带教环节精准定位提高儿科转岗医师培训效果

中国继续医学教育 2020年第23期12卷 9-13页

作者：彭晓康李小青陈伟超张美慧樊华峰黄杰孙烁武海滨西安市儿童医院科教科陕西西安710003

目的评价提高带教环节对儿科转岗医师的定位是否能够提高培训效果。方法向带教老师宣讲儿科转岗医师工作基础等信息使其对转岗医师有精确定位;2018年调整教学重视临床工作融入,优化教学方案;对比改革前后2年转岗医师结业考核成绩。结果2... 详细信息

目的评价提高带教环节对儿科转岗医师的定位是否能够提高培训效果。方法向带教老师宣讲儿科转岗医师工作基础等信息使其对转岗医师有精确定位;2018年调整教学重视临床工作融入,优化教学方案;对比改革前后2年转岗医师结业考核成绩。结果2017年组共47名学员,男:女=16:31,年龄(35.28±6.78)岁,工作年限(12.68±7.27)年;2018年组共42名学员,男:女=13:29,(35.86±6.06)岁,工作年限(13.24±6.63)年。两组年龄(t=0.180,P=0.673),职称(χ^2=2.262,P=0.136),工作年限(t=0.142,P=0.708)差异均无统计学意义。2017年和2018年结业成绩分别为(73.1±7.3)分和(75.8±4.4)分,差异有统计学意义。结论带教环节精准定位儿科转岗医师工作基础、就业意向,融教学于临床实践针对性地培训能有效提高儿科转岗医师培训效果。

关键词：儿科转岗医师教学方法临床就业定位

Material patterning on substrates by manipulation of fluidic behavior

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National Science Review 2019年第4期6卷 758-766页

作者： Yitan Li Hao Wang Henglu Xu Shiting Wu Xuemei Li Jiapeng Yu Chaoyu Huang Zeyao zhang Hao Sun Lu Han meihui Li Anyuan Cao Zhenhai Pan Yan Li Key Laboratory for the Physics and Chemistry of Nanodevices Beijing National Laboratory of Molecular SciencesState Key Laboratory of Rare Earth Materials Chemistry and Applications College of Chemistry and Molecular Engineering Peking University Academy for Advanced Interdisciplinary Studies Peking University College of Engineering Peking University Electron Microscopy Laboratory Peking University Bruker (Beijing) Scientific Technology Co. Ltd. 6. Institute of Engineering ThermophysicsShanghai Jiao Tong University

Patterned materials on substrates are of great importance for a wide variety of applications. In solution-based approaches to material patterning, fluidic flow is inevitable. Here we demonstrate not only the importance of fluidic behavior but also the methodology of engineering the flow pattern to guide the material crystallization and assembly. We show by both experiment and simulation that substrate heating,which is generally used to accelerate evaporation, produces irregular complex vortexes. Instead, a top-heating–bottom-cooling(THBC) set-up offers an inverse temperature gradient and results in a single Marangoni vortex, which is desired for ordered nanomaterial patterning near the contact line. We then realize the fabrication of large-scale patterns of iodide perovskite crystals on different substrates under THBC conditions. We further demonstrate that harnessing the flow behavior is a general strategy with great feasibility to pattern various functional materials ranging from inorganic, organic, hybrid to biological categories on different substrates, presenting great potential for practical applications.

关键词： Marangoni flow temperature gradient large-scale material patterning flexible device contact line

痘苗病毒天坛株N1L基因表达调控序列的克隆与特性分析

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病毒学报 2024年第3期40卷 484-491页

作者：翟玉倩韩一泽吴长城张钟贤赵莉罗美惠豆亚美刘士元任皎田厚文王文玲谭文杰中国疾病预防控制中心病毒病预防控制所传染病溯源预警和智能决策全国重点实验室北京102206

本研究以痘苗病毒天坛株(Vaccinia Virus Tian Tan Strain,VTT)为对象,旨在明确N1L基因的表达调控序列。我们克隆了N1L起始密码子ATG上游不同长度的调控序列片段,构建了表达载体,并利用报告基因萤火虫荧光素酶(Firefly luciferase,Fluc... 详细信息

本研究以痘苗病毒天坛株(Vaccinia Virus Tian Tan Strain,VTT)为对象,旨在明确N1L基因的表达调控序列。我们克隆了N1L起始密码子ATG上游不同长度的调控序列片段,构建了表达载体,并利用报告基因萤火虫荧光素酶(Firefly luciferase,Fluc)的表达来确定N1L表达调控序列区域。通过PROMO在线预测网站,预测了与调控序列结合的转录因子。结果显示,VTT中N1L基因的表达受到上游调控序列的调控,其中以-404bp起始的片段启动的Fluc表达量最高。PROMO预测表明该区域存在多个转录因子结合位点。本研究为痘苗病毒基因表达特点的研究提供了实验依据,并为N1L基因功能及机制研究奠定了基础。

关键词：痘苗病毒天坛株 N1L基因基因表达与调控转录通读

Production of XX neo-male mandarin fish, Siniperca chuatsi, after sexual differentiation by oral administration of 17α-methyltestosterone

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Water Biology and Security 2023年第1期2卷 96-103页

作者： Jingjun Huang Shiyan Liu Haofeng Ouyang Chong Han meihui Li Linqiang Han Shuisheng Li Guifeng Li Haoran Lin Yong zhang State Key Laboratory of Biocontrol and School of Life Sciences Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic FishSun Yat-Sen UniversityGuangzhou510275PR China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao266373PR China Guangdong Liangshi Aquatic Seed Industry Co. LtdFoshan528100PR China

A well-known fact about mandarin fish(Siniperca chuatsi)is that females grow faster than *** fish that have all-female populations have economic advantages,but traditional neo-male fish are difficult to *** this study,indirect feeding of 500 mg/kg 17α-methyltestosterone(MT)to XX mandarin fish(60 dpf,days post fertilization)with completely differentiated ovaries induced secondary sex *** 40 days after MT treatment,14.7%of mandarin fish with complete reversal was produced,which showed for the first time that XX mandarin fishes with differentiated ovaries still have potentially bisexual germ cells and the plasticity of sex *** the MT treatment group,there was no significant difference in survival compared to the control *** body weight of the MT treatment group was significantly lower than that of the control group 40 days after treatment(dat)(P<0.05).During MT treatment,serum 11-KT and T concentrations increased and serum E2 concentration decreased in the MT treatment group,but there was no significant difference in hormone levels between the MT treatment group and the control group.A masculinization of mandarin fish was also confirmed by the expression patterns of sex-specific genes sox9,dmrt1,cyp19a1a and foxl2.A significant amount of sox9 and dmrt1 was expressed in the fish gonads of the MT treatment group,whereas cyp19a1a and foxl2 were minimally expressed in the fish gonads.

关键词： 17α-methyltestosterone(MT) Mandarin fish Sex reversal Sex-specific genes

Detecting SARS-CoV-2 BA.2,BA.4,and BA.5 Variants Utilizing a Robust RT-RPA-CRISPR/Cas12a-Based Method-China,2023

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China CDC weekly 2023年第26期5卷 584-591页

作者： meihui Luo Yang Pan Yaqing He Ruhan A Changcheng Wu Baoying Huang Roujian Lu Li Zhao Bo Peng Fei Ye Huijuan Wang Yuda Chen Zhen Li Daitao zhang Wenling Wang Wenjie Tan MHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChina CDCBeijingChina Beijing Center for Disease Prevention and Control BeijingChina Microbiology Laboratory Shenzhen Center for Disease Control and PreventionShenzhen CityGuangdong ProvinceChina School of Public Health Baotou Medical CollegeBaotou CityInner Mongolia Autonomous RegionChina Collaborative Innovation Centre for Regenerative Medicine and Medical BioResourse Development and Application Co-constructed by the Province and Ministry Guangxi Medical UniversityGuangxi Zhuang Autonomous RegionChina

Introduction:Since 2019,numerous variants of concern for severe acute respiratory syndrome virus 2(SARS-CoV-2)have emerged,leading to significant *** development of novel,highly accurate,and rapid detection techniques for these new SARSCoV-2 variants remains a primary focus in the ongoing efforts to control and prevent the coronavirus disease 2019(COVID-19)***:Reverse transcription-recombinase polymerase amplification combined with the clustered regularly interspaced short palindromic repeatsassociated protein 12a(CRISPR/Cas12a)system was used to validate the detection of the Omicron BA.2,BA.4,and BA.5 variants of ***:Our results demonstrate that the CRISPR/Cas12a assay is capable of effectively detecting the SARS-CoV-2 BA.2,BA.4,and BA.5 variants with a limit of detection of 10,1,and 10 copies/μL,***,our assay successfully differentiated the three SARS-CoV-2 Omicron strains from one ***,we evaluated 46 SARS-CoV-2 positive clinical samples consisting of BA.2(n=20),BA.4(n=6),and BA.5(n=20)variants,and the sensitivity of our assay ranged from 90%to 100%,while the specificity was 100%.Discussion:This research presents a swift and reliable CRISPR-based method that may be employed to track the emergence of novel SARS-CoV-2 variants.

关键词： CRISPR/Cas limit specificity

Three-dimensional quantication of protoporphyrin IX in photodynamic therapy using SFDI/DFT:A pilot experimental validation

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Journal of Innovative Optical Health Sciences 2022年第6期15卷 30-39页

作者： Mai Dan Weijie Song meihui Liu Yaru zhang Feng Gao College of Precision Instrument and Optoelectronics Engineering Tianjin University Tianjin 300072 P.R.China Tianjin Medical University Cancer Institute and Hospital Tianjin 300000 P.R.China Tianjin Key Laboratory of Biomedical Detecting Techniques and Instruments Tianjin 300072 P.R.China

Photodynamic therapy(PDT)dosimetry,induding light dose,photosensitizer dose and tissue oxygen,has been a research focus in *** this work,we present a three-dimensional(3D)quantification of protoporphyrin X(PpIX)using combined spatial frequency domain imaging(SFDI)and diffuse fuorescence tomography(DFT).The SFDI maps both the distributions of tissue absorption and scattering properties at three wavelengths and accordingly provides the optical background for DFT and extracts the tissue oxygenation for assessing the therapeutic outcomes,while DFT dynamically monitors the 3D distribution of PpEX dose from measured fluorescence signals for the procedure optimization.A pilot in vrivo application in tumor nude models showed that the proposed SFDI/DFT is able to dynamically trace changes in the PpX concentration and tissue oaxygen during the treatment,rendering it a potentially powerful tool for PDT to improve clinical eficacy.

关键词： Photodynamic therapy spatial frequency domain imaging difuse fuorescence tomography