A micro-nano optogenetic system based on probiotics for in situ host metabolism regulation

作     者：Xinyu Zhang Ning Ma Wei Ling Gaoju Pang Tao Sun Jing Liu Huizhuo Pan Meihui Cui Chunli Han Chun Yang Jin Chang Xian Huang Hanjie Wang Xinyu Zhang;Ning Ma;Wei Ling;Gaoju Pang;Tao Sun;Jing Liu;Huizhuo Pan;Meihui Cui;Chunli Han;Chun Yang;Jin Chang;Xian Huang;Hanjie Wang

作者机构：School of Life SciencesTianjin UniversityTianjin 300072China School of Precision Instrument and Optoelectronics EngineeringTianjin UniversityTianjin 300072China School of Chemical Engineering&TechnologyTianjin UniversityTianjin 300072China 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2023年第16卷第2期

页      面：2829-2839页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：sponsored by the National Science Fund for Excellent Young Scholars(No.32122047) the National Key Research and Development Program of China(No.2019YFA0906500) the National Natural Science Foundation of China(Nos.31971300 and 51873150) the Key project of Tianjin Foundational Research(JingJinJi)Program,China(No.19JCZDJC64100) the Key Research and Development Program of Tianjin(No.19YFZCSY00190) the National Science Foundation of Tianjin(No.20YDTPJC00090) 

主　　题：synthetic biology optogenetics upconversion nanoparticles microelectronics diabetics 

摘      要：Genetically engineered bacteria have aroused attention as micro-nano drug delivery systems in ***,conventional designs of engineered bacteria usually function constantly or autonomously,which might be non-specific or ***,designing and optimizing in situ control strategy are important methodological progress for therapeutic researches of intestinal engineered ***,a micro-nano optogenetic system based on probiotic was developed combining microelectronics,nanotechnology,and synthetic biology to achieve in situ controllable drug ***,optogenetic engineered Lactococcus lactis was orally administrated in the intestinal tract.A wearable optical device was designed to control optical signals ***,*** could be customized to secrete peptides according to optical *** an example,optogenetic *** system can be constructed to secrete glucagon-like peptide-1(GLP-1)under the control of the wearable optical device to regulate *** improve the half-life of GLP-1 in vivo,Fc-domain fused GLP-1 was optimally *** this strategy,blood glucose,weight,and other features were well controlled in rats and mice ***,upconversion microcapsules were introduced to increase the excitation wavelength of the optogenetic system for better *** strategy has biomedical potential to expand the toolbox for intestinal engineered bacteria.