检索结果-南通市图书馆

An on-line multi-parameter analyzing optical biosensor for real-time and non-invasive monitoring of plant stress responses in vivo

引用

Chinese Science Bulletin 2009年第21期54卷 4009-4016页

作者： zhang lingrui xing da wen feng MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science South China Normal University Guangzhou 510631China

Photosynthetic dysfunction and reactive oxygen species (ROS) production are the common features of plant stress responses. Based on quantitative measurement of ROS production and delayed fluorescence (DF) emission, which is an excellent marker for evaluating photosynthesis, an on-line multiparameter analyzing optical biosensor for detecting plant stress responses was developed. Performances of the proposed biosensor were tested in the wild type (WT) Arabidopsis and heat shock protein (Hsp) 101 T-DNA knockout mutant (hsp101) plants with different thermotolerance. Results demonstrated that DF intensity correlates with net photosynthesis rate (Pn) in response to elevated temperature in both the WT Arabidopsis and hsp101 mutant plants. The light response characteristics and the recovery dynamics of the DF intensity were also in line with those of Pn in both the WT Arabidopsis and hsp101 mutant plants after heat stress (HS, 40℃ for 30 min), respectively. In all experiments discussed above, the hsp101 plant showed the worse photosynthetic performance than the WT plant. Moreover, after HS, more ROS production in the hsp101 mutant than in WT Arabidopsis, which was found to be mainly localized at chloroplasts, could be directly detected by using the proposed biosensor. In addition, the hsp101 mutant showed severer chloroplasts alterations than the WT plant within the first 1 h of recovery following HS. Nevertheless, pre-infiltration with catalase (CAT) reduced ROS production and prevented the declines of the DF intensity. Therefore, HS-caused declines of photosynthetic performance might be due to oxidative damage to photosynthetic organelle. To sum up, we conclude that Hsp101 plays an important role in preventing oxidative stress, and the proposed optical biosensor might be a powerful tool to determine plant stress responses and identify plant resistant difference.

关键词：光学传感器多参数分析应激反应侵入性监测植物实时体内

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Liver-infiltrating CD11b−CD27− NK subsets account for NK-cell dysfunction in patients with hepatocellular carcinoma and are associated with tumor progression

引用

Cellular & Molecular Immunology 2017年第10期14卷 819-829页

作者： Qiong-Fang zhang wen-Wei Yin Yang Xia Ya-Yang Yi Qiu-feng He xing Wang Hong Ren da-Zhi zhang Key Laboratory of Molecular Biology for Infectious Diseases(Ministry of Education) Institute for Viral HepatitisDepartment of Infectious DiseasesThe Second Affiliated HospitalChongqing Medical UniversityChongqing 400016PR China Department of Urinary Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China Department of Hepatobiliary Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China Department of Orthopaedics Surgery The Second Affiliated Hospital of Chongqing Medical UniversityChongqing 400010PR China

Natural killer(NK)cells have a vital role in killing hepatocellular carcinoma(HCC)cells;however,the mechanism underlying tumor-infiltrating NK(TINK)-cell dysfunction remains poorly *** flow cytometry staining,we precisely characterized the frequency,phenotype and function of NK subsets distinguished by CD27 and CD11b in 30 patients with HCC in comparison to 30 healthy ***,we found a substantial proportion of liver-infiltrating CD11b−CD27−(DN)NK subsets in tumor tissue from HCC ***,these relatively expanded DN NK subsets exhibited an inactive and immature *** detecting the expression of CD107a and interferon-gamma(IFN-γ)on NK subsets and NK cells,we demonstrated that DN NK subsets exhibited a poor cytotoxic capacity and deficient potential to produce IFN-γin comparison to the other three subsets,which contributed to the dysfunction of TINK cells in HCC *** addition,we found that the presence of DN NK cells was closely associated with the clinical outcomes of HCC patients,as the frequency of DN NK cells among TINK cells was positively correlated with tumor stage and size.A large percentage of DN NK cells among TINK cells was an independent prognostic factor for lower survival in the 60-month follow-up *** conclusion,a substantial proportion of CD11b−CD27−NK subsets among TINK cells accounts for NK-cell dysfunction in patients with HCC and is associated with tumor *** study may provide a novel therapeutic target for the treatment of patients with HCC.

关键词： CD11b CD27 dysfunction NK subsets

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

One-pot synthesis of Pd-Au-alloy-nanoparticle-decorated graphene oxide functionalized with dodecahydrododecaborate cluster for rapid and complete reduction of 4-nitrophenol at room temperature

引用

Rare Metals 2023年第11期42卷 3622-3629页

作者： Yu-Bo Ma Yu-wen Wang da-feng zhang Xiu-Xiu Jia Yin Wang Shu-xing Zhou Thomas Wagberg Guang-Zhi Hu School of Chemical Engineering Chongqing Chemical Industry Vocational CollegeChongqing401220China School of Materials and Energy Institute for Ecological Research and Pollution Control of Plateau LakesYunnan UniversityKunming650504China School of Materials Science and Engineering Liaocheng UniversityLiaocheng252000China Hubei Key Laboratory of Low Dimensional Optoelectronic Materials and Devices Hubei University of Arts and ScienceXiangyang441053China Department of Physics UmeåUniversity90187UmeåSweden

The treatment of nitroaromatics in wastewater is a significant issue that must be addressed because of their high *** catalytic reduction of 4-nitrophenol(4-NP)to the widely used 4-aminophenol(4-AP)is an excellent method for 4-NP ***,performing this reaction is *** this study,two-dimensional(2D)graphene oxide(GO)with oxygen-containing surface groups was used as a substrate for the in situ anchoring ofPdau alloy nanocrystals.

关键词： alloy rapid cluster

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Inhibitory Effect of Jingui Shenqi Pills on Glucocorticoid-Enhanced Axial Length Elongation in Experimentally Myopic Guinea Pigs

引用

Chinese Journal of Integrative Medicine 2023年第11期29卷 989-997页

作者： JIANG Qian JIANG wen-jun YANG Cheng-xiu zhang Rui-xue SUN Wei GUO da-dong WU Jiang-feng GUO Bin WANG xing-rong BI Hong-sheng Department of Ophthalmology and Optometry Shandong University of Traditional Chinese MedicineJinan(250014)China Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine Jinan(250002)China Shandong Province Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Disease Shandong Academy of Eye Disease Prevention and TherapyJinan(250002)China

Objective:To explore the underlying mechanism of inhibition by Jingui Shenqi Pills(JGSQP)on glucocorticoid-enhanced axial length elongation in experimental lens-induced myopia(LIM)guinea ***:Sixty 2-week old male guinea pigs were randomly divided into 4 groups with 15 guinea pigs in each group,according to the random numbers generated by SPSS software:control,LIM,saline and JGSQP *** control group includes animals with no treatment,while the guinea pigs in the other 3 groups received lens-induced myopization on the right eyes throughout the experiment(for 8 weeks).The saline and JGSQP groups were given daily intraperitoneal injections of 10 mg/kg hydrocortisone for 2 consecutive weeks at the same time,and then orally administered either saline or JGSQP 13.5 g/(kg·d)for 6 consecutive *** weight,anal temperature and animal appearance were observed and recorded to evaluate the GC-associated *** ocular parameters,including refraction and axial length,were measured by streak retinoscopy and A-scan ultrasonography,*** levels of plasma hormones associated with the hypothalamic-pituitary-adrenal axis(HPAA),including free triiodothyronine,free thyroxine,estradiol and testosterone,were measured by radioimmunoassay,and cyclic adenosine monophosphate(cAMP)and cyclic guanosine monophosphate were measured by enzyme-linked immunosorbent *** addition,the mRNA and protein expressions of retinal amphiregulin(AREG)was measured by quantitative real-time polymerase chain reaction and Western blotting,***:JGSQP effectively increased body weight and anal temperature,improved animal appearance and suppressed axial length elongation in glucocorticoidenhanced myopic guinea pigs with normalization of 4 HPAA-associated plasma hormones(all P<0.05).The plasma level of cAMP was significantly increased,whereas the plasma level of cGMP and the mRNA and protein expressions of retinal AREG were decreased after treatment with JGSQP(all

关键词： guinea treatment normalized

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genome-wide identification and function analysis of the sucrose phosphate synthase MdSPS gene family in apple

引用

Journal of Integrative Agriculture 2023年第7期22卷 2080-2093页

作者： zhang Li-hua ZHU Ling-cheng XU Yu LÜLong LI xing-guo LI wen-hui LIU Wan-da MA feng-wang LI Ming-jun HAN De-guo The Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region) Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold RegionsCollege of Horticulture&Landscape ArchitectureNortheast Agricultural UniversityHarbin 150030P.R.China State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple College of HorticultureNorthwest A&F UniversityYangling 712100P.R.China Horticulture Branch Heilongjiang Academy of Agricultural SciencesHarbin 150040P.R.China

Sucrose phosphate synthase(SPS)is a rate-limiting enzyme that works in conjunction with sucrose-6-phosphate phosphatase(SPP)for sucrose synthesis,and it plays an essential role in energy provisioning during growth and development in plants as well as improving fruit ***,studies on the systematic analysis and evolutionary pattern of the SPS gene family in apple are still *** the present study,a total of seven MdSPS and four MdSPP genes were identified from the Malus domestica genome GDDH13 *** gene structures and their promoter cis-elements,protein conserved motifs,subcellular localizations,physiological functions and biochemical properties were analyzed.A chromosomal location and gene-duplication analysis demonstrated that whole-genome duplication(WGD)and segmental duplication played vital roles in MdSPS gene family *** Ka/Ks ratio of pairwise MdSPS genes indicated that the members of this family have undergone strong purifying selection during ***,three SPS gene subfamilies were classified based on phylogenetic relationships,and old gene duplications and significantly divergent evolutionary rates were observed among the SPS gene *** addition,a major gene related to sucrose accumulation(MdSPSA2.3)was identified according to the highly consistent trends in the changes of its expression in four apple varieties(‘Golden Delicious’,‘Fuji’,‘Qinguan’and‘Honeycrisp’)and the correlation between gene expression and soluble sugar content during fruit ***,the virus-induced silencing of MdSPSA2.3 confirmed its function in sucrose accumulation in apple *** present study lays a theoretical foundation for better clarifying the biological functions of the MdSPS genes during apple fruit development.

关键词： apple sucrose phosphate synthase evolutionary pattern expression profile sugar accumulation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Commissioning the photocathode radio frequency gun:a candidate electron source for Hefei Advanced Light Facility

引用

Nuclear Science and Techniques 2022年第3期33卷 1-9页

作者： wen-xing Wang Cheng Li Zhi-Gang He Shan-Cai zhang Rui-Xuan Huang Wei-Wei Li Ping Lu Chuan Li Lei-Lei Tang feng-Lei Shang Fang-Fang Wu da-Chun Jia Hai-Yan zhang Wei Wang National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefei 230029China

The Hefei Advanced Light Facility(HALF)proposed by the National Synchrotron Radiation Laboratory(NSRL)is a diffraction-limited storage ring(DLSR),which plans to use a full energy linac as the *** ensure injection efficiency,the injection beam needs to have low ***,a photocathode radio frequency(RF)gun was developed in the HALF R&D *** gun is designed to deliver high-quality electron bunches with a typically 0.5 nC charge and~4.5 MeV energy with low *** initial system commission with an electron beam was completed at the end of 2020,and a stable 1.2–1.4 *** emittance with a bunch charge of 500 pC was *** this paper,we report the experimental results and experience obtained during the commission,including the RF gun,drive laser system,and beam diagnostics.

关键词： High-brightness electron beam Photoinjector laser shaping Transverse emittance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genomic Epidemiology of Imported Cases of COVID-19 in Guangdong Province,China,October 2020–May 2021

引用

Biomedical and Environmental Sciences 2022年第5期35卷 393-401页

作者： LIANG dan WANG Tao LI Jiao Jiao GUAN da Wei zhang Guan Ting LIANG Yu feng LI An An HONG wen Shan WANG Li CHEN Meng Lin DENG Xiao Ling CHEN feng Juan PAN xing Fei JIA Hong Ling LEI Chun Liang KE Chang wen Guangdong Provincial Key Laboratory of Virology Institute of Medical MicrobiologyJinan UniversityGuangzhou 510632GuangdongChina Guangzhou National Laboratory Guangzhou 510700GuangdongChina Guangdong Provincial Center for disease control and prevention Guangdong Workstation for Emerging Infectious Disease Control and PreventionGuangzhou 511430GuangdongChina Shantou University Medical College Shantou 515041GuangdongChina Guangzhou Eighth People’s Hospital Guangzhou 510030GuangdongChina School of public health Sun Yat-sen UniversityGuangzhou 510080GuangdongChina School of Public Health Southern Medical UniversityGuangzhou 510515GuangdongChina Guangzhou Mendel Genomics and Medical Technology Co. Ltd.Guangzhou 510535GuangdongChina Department of Infectious Diseases the Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510150GuangdongChina Department of Medical Biochemistry and Molecular Biology School of MedicineJinan UniversityGuangzhou 510632GuangdongChina

Objective The pandemic of severe acute respiratory syndrome coronavirus 2(SARS-Co V-2) has been engendering enormous hazards to the world. We obtained the complete genome sequences of SARSCo V-2 from imported cases admitted to the Guangzhou Eighth People’s Hospital, which was appointed by the Guangdong provincial government to treat coronavirus disease 2019(COVID-19). The SARS-Co V-2 diversity was analyzed, and the mutation characteristics, time, and regional trend of variant emergence were *** In total, 177 throat swab samples were obtained from COVID-19 patients(from October2020 to May 2021). High-throughput sequencing technology was used to detect the viral sequences of patients infected with SARS-Co V-2. Phylogenetic and molecular evolutionary analyses were used to evaluate the mutation characteristics and the time and regional trends of *** We observed that the imported cases mainly occurred after January 2021, peaking in May2021, with the highest proportion observed from cases originating from the United States. The main lineages were found in Europe, Africa, and North America, and B.1.1.7 and B.1.351 were the two major sublineages. Sublineage B.1.618 was the Asian lineage(Indian) found in this study, and B.1.1.228 was not included in the lineage list of the Pangolin web. A reasonably high homology was observed among all samples. The total frequency of mutations showed that the open reading frame 1 a(ORF1 a) protein had the highest mutation density at the nucleotide level, and the D614 G mutation in the spike protein was the commonest at the amino acid level. Most importantly, we identified some amino acid mutations in positions S, ORF7 b, and ORF9 b, and they have neither been reported on the Global Initiative of Sharing All Influenza data nor published in Pub Med among all missense *** These results suggested the diversity of lineages and sublineages and the high homology at the amino acid level among imported case

关键词： Severe acute respiratory syndrome coronavirus 2 Next-Generation sequencing Phylogenetic tree Variants

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Development and validation of a model for predicting wheel wear in high-speed trains

引用

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2017年第8期18卷 603-616页

作者： Gong-quan TAO xing DU He-ji zhang Ze-feng wen Xue-song JIN da-bin CUI State Key Laboratory of Traction Power Southwest Jiaotong University Chengdu 610031 China Department of Mechanical Engineering Emei Campus of Southwest Jiaotong University Emei 614202 China

In this paper, we present a comprehensive model for the prediction of the evolution of high-speed train wheel profiles due to wear. The model consists of four modules： a multi-body model implemented with the commercial multi-body software SIMPACK to evaluate the dynamic response of the vehicle and track; a local contact model based on Hertzian theory and a novel method, named FaStrip （Sichani et al., 2016）, to calculate the normal and tangential forces, respectively; a wear model proposed by the University of Sheffield （known as the USFD wear function） to estimate the amount of material removed and its distribution along the wheel profile; and a smoothing and updating strategy. A simulation of the wheel wear of the high-speed train CRH3 in service on the Wuhan-Guangzhou railway line was performed. A virtual railway line based on the statistics of the line was used to represent the entire real track. The model was validated using the wheel wear data of the CRH3 operating on the Wuhan- Guangzhou line, monitored by the authors＇ research group. The results of the predictions and measurements were in good agreement.

关键词： High-speed train Wheel profile Wheel/Rail contact Wheel wear prediction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Canonical transient receptor potential channel 1 aggravates myocardial ischemia-and-reperfusion injury by upregulating reactive oxygen species

引用

Journal of Pharmaceutical Analysis 2023年第11期13卷 1309-1325页

作者： Hui-Nan zhang Meng zhang wen Tian Wei Quan Fan Song Shao-Yuan Liu Xiao-Xiao Liu dan Mo Yang Sun Yuan-Yuan Gao wen Ye Ying-da feng Chang-Yang xing Chen Ye Lei Zhou Jing-Ru Meng Wei Cao Xiao-Qiang Li Department of Health Management Second Affiliated HospitalFourth Military Medical UniversityXi'an710038China Department of Pharmacology School of PharmacyFourth Military Medical UniversityXi'an710032China Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine School of PharmacyFourth Military Medical UniversityXi'an710032China Shaanxi Key Laboratory of“Qin Medicine”Research and Development Shaanxi Administration of Traditional Chinese MedicineXi'an710032China Institute of Materia Medica School of PharmacyFourth Military Medical UniversityXi'an710032China Department of Ultrasound Diagnostics Second Affiliated HospitalFourth Military Medical UniversityXi'an710038China Department of Pharmacy School of Chemistry&PharmacyNorthwest A&F UniversityYanglingShaanxi712100China

The canonical transient receptor potential channel(TRPC)proteins form Ca^(2+)-permeable cation channels that are involved in various heart ***,the roles of specific TRPC proteins in myocardial ischemia/reperfusion(I/R)injury remain poorly *** observed that TRPC1 and TRPC6 were highly expressed in the area at risk(AAR)in a coronary artery ligation induced I/R ***1/mice exhibited improved cardiac function,lower serum Troponin T and serum creatine kinase level,smaller infarct volume,less fibrotic scars,and fewer apoptotic cells after myocardial-I/R than wild-type or Trpc6/***-specific knockdown of Trpc1 using adeno-associated virus 9 mitigated myocardial I/R ***,Trpc1 deficiency protected adult mouse ventricular myocytes(AMVMs)and HL-1 cells from death during hypoxia/reoxygenation(H/R)***-sequencing-based transcriptome analysis revealed differential expression of genes related to reactive oxygen species(ROS)generation in Trpc1/*** these genes,oxoglutarate dehydrogenase-like(Ogdhl)was markedly ***,Trpc1 deficiency impaired the calcineurin(CaN)/nuclear factorkappa B(NF-kB)signaling pathway in *** of this pathway inhibited Ogdhl upregulation and ROS generation in HL-1 cells under H/R *** immunoprecipitation assays confirmed NF-kB binding to the Ogdhl *** cardioprotective effect of Trpc1 deficiency was canceled out by overexpression of NF-kB and Ogdhl in *** conclusion,our findings reveal that TRPC1 is upregulated in the AAR following myocardial I/R,leading to increased Ca^(2+) influx into associated ***,this upregulates Ogdhl expression through the CaN/NF-kB signaling pathway,ultimately exacerbating ROS production and aggravating myocardial I/R injury.

关键词： TRPC1 Myocardial ischemia/reperfusion Reactive oxygen species OGDHL

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Construction of a telomerase-immortalized porcine tracheal epithelial cell model for swine-origin mycoplasma infection

引用

Journal of Integrative Agriculture 2022年第2期21卷 504-520页

作者： XIE xing HAO Fei WANG Hai-yan PANG Mao-da GAN Yuan LIU Bei-bei zhang Lei WEI Yan-na CHEN Rong zhang Zhen-zhen BAO wen-bin BAI Yun SHAO Guo-qing XIONG Qi-yan feng Zhi-xin Key Laboratory for Veterinary Bio-Product Engineering of Ministry of Agriculture and Rural Affairs Institute of Veterinary MedicineJiangsu Academy of Agricultural SciencesNanjing 210014P.R.China Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology Institute of Food Safety and NutritionJiangsu Academy of Agricultural SciencesNanjing 210014P.R.China College of Animal Science and Technology Yangzhou UniversityYangzhou 225009P.R.China

Primary porcine tracheal epithelial cells(PTECs) are an appropriate model for studying the molecular mechanism of various porcine respiratory diseases, including swine-origin mycoplasmas, which are isolated from respiratory tract of pigs and mainly found on the mucosal surface surrounding swine trachea. However, the short proliferation ability of primary PTECs greatly limits their lifespan. In this study, primary PTECs were carefully isolated and cultured, and immortal PTECs were constructed by transfecting primary PTECs with the recombinant constructed plasmid pEGFPhTERT containing human telomerase reverse transcriptase(hTERT). Immortal PTECs(hTERT-PTECs) maintained both the morphological and functional characteristics of primary PTECs, as indicated by the expression of cytokeratin 18, cellcycle analysis, proliferation assay, Western blotting, telomerase activity assay, karyotype analysis and quantitative RTPCR. Compared to primary PTECs, hTERT-PTECs had an extended replicative lifespan, higher telomerase activity, and enhanced proliferative activity. In addition, this cell line resulted in a lack of transformed and grown tumors in nude mice, suggesting that it could be safely applied in further studies. Moreover, hTERT-PTECs were vulnerable to all swineorigin mycoplasmas through quantitative analysis as indicated by 50% color changing unit(CCU_(50)) calculation, and no significant differences of adhesion ability between primary and immortal PTECs were observed. For the representative swine mycoplasma Mycoplasma hyopneumoniae(Mhp), except for DNA copies quantitative real-time PCR assay, indirect immunofluorescence assay and Western blotting analysis also depicted that hTERT-PTECs was able to adhere to different Mhp strains of different virulence. In summary, like primary PTECs, hTERT-PTECs could be widely used as an adhesion cell model for swine-origin mycoplasmas and in infection studies of various porcine respiratory pathogens.

关键词： porcine tracheal epithelial cells(PTECs) hTERT-PTECs swine-origin mycoplasmas adhesion cell model

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：