Genome-wide identification and function analysis of the sucrose phosphate synthase MdSPS gene family in apple

作     者：ZHANG Li-hua ZHU Ling-cheng XU Yu LÜLong LI Xing-guo LI Wen-hui LIU Wan-da MA Feng-wang LI Ming-jun HAN De-guo ZHANG Li-hua;ZHU Ling-cheng;XU Yu;Lü Long;LI Xing-guo;LI Wen-hui;LIU Wan-da;MA Feng-wang;LI Ming-jun;HAN De-guo

作者机构：The Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region)Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold RegionsCollege of Horticulture&Landscape ArchitectureNortheast Agricultural UniversityHarbin 150030P.R.China State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of AppleCollege of HorticultureNorthwest A&F UniversityYangling 712100P.R.China Horticulture BranchHeilongjiang Academy of Agricultural SciencesHarbin 150040P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2023年第22卷第7期

页      面：2080-2093页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the National Natural Science Foundation of China (32172521) the Excellent Youth Science Foundation of Heilongjiang Province,China (YQ2023C006) the Talent Introduction Program of Northeast Agricultural University of China the Collaborative Innovation System of the Agricultural Bio-economy in Heilongjiang Province,China 

主　　题：apple sucrose phosphate synthase evolutionary pattern expression profile sugar accumulation 

摘      要：Sucrose phosphate synthase(SPS)is a rate-limiting enzyme that works in conjunction with sucrose-6-phosphate phosphatase(SPP)for sucrose synthesis,and it plays an essential role in energy provisioning during growth and development in plants as well as improving fruit ***,studies on the systematic analysis and evolutionary pattern of the SPS gene family in apple are still *** the present study,a total of seven MdSPS and four MdSPP genes were identified from the Malus domestica genome GDDH13 *** gene structures and their promoter cis-elements,protein conserved motifs,subcellular localizations,physiological functions and biochemical properties were analyzed.A chromosomal location and gene-duplication analysis demonstrated that whole-genome duplication(WGD)and segmental duplication played vital roles in MdSPS gene family *** Ka/Ks ratio of pairwise MdSPS genes indicated that the members of this family have undergone strong purifying selection during ***,three SPS gene subfamilies were classified based on phylogenetic relationships,and old gene duplications and significantly divergent evolutionary rates were observed among the SPS gene *** addition,a major gene related to sucrose accumulation(MdSPSA2.3)was identified according to the highly consistent trends in the changes of its expression in four apple varieties(‘Golden Delicious’,‘Fuji’,‘Qinguan’and‘Honeycrisp’)and the correlation between gene expression and soluble sugar content during fruit ***,the virus-induced silencing of MdSPSA2.3 confirmed its function in sucrose accumulation in apple *** present study lays a theoretical foundation for better clarifying the biological functions of the MdSPS genes during apple fruit development.