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Cross-talking between autophagy and viral infection in mammalian cells

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Frontiers in Biology 2010年第6期5卷 507-515页

作者： Hongya HAN lishu zhang Xinxian DAI Yanpeng ZHENG College of Life Sciences&Bioengineering Beijing Jiaotong UniversityBeijing 100044China

Autophagy is a cellular process in degradation of long-lived proteins and organelles in the cytosol for maintaining cellular homeostasis,which has been linked to a wide range of human health and disease states,including viral *** viral infected cells exhibit a compli-cated cross-talking between autophagy and *** has been shown that autophagy interacts with both adaptive and innate *** adaptive immunity,viral antigens can be processed in autophagosomes by acidic proteases before major histocompatibility complex(MHC)class II *** innate immunity,autophagy may assist in the delivery of viral nucleic acids to endosomal TLRs and also functions as a part of the TLR-or-PKR-downstream *** was also reported to suppress the magnitude of host innate antiviral immunity in certain *** the other hand,viruses has evolved many strategies to combat or utilize the host autophagy for their own beneﬁ*** this review we discussed recent advances toward clarifying the cross-talking between autophagy and viral infection in mammalian cells.

关键词： cross-talking autophagy viral infection

MyD88 in macrophages protects against colitis via inhibiting the activation of NLRP3 inflammasome in epithelial cells

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Genes & Diseases 2023年第2期10卷 344-347页

作者： Shi Liu Yanmin Wu Haiqiang Chen Qi Yuan Shuang Ge lishu zhang Lingling Hou Tian Tian Jinhua zhang College of Life Science and Bioengineering Beijing Jiaotong UniversityBeijing 100044China National Center for International Research of Bio-targeting Theranostics Guangxi Key Laboratory of Bio-targeting TheranosticsCollaborative Innovation Center for Targeting Tumor Diagnosis and TherapyGuangxi Talent Highland of Bio-targeting TheranosticsGuangxi Medical UniversityNanningGuangxi 530021China

The signal adaptor myeloid differentiation primary response 88(MyD88)of Toll-like receptor(TLR)signaling is universally expressed in immune cells and non-immune cells,and myeloid cells play a significant role in modulating *** are myeloid lineage cells which is important for maintaining intestinal homeostasis in inflammation.1 And macrophages can recognize invading pathogens through pattern recognition receptors(PRRs),such as TLRs and the nucleotide oligomerization domain(NOD)-like receptor family,pyrin domain-containing 3(NLRP3),and quickly infiltrate the injured site leading to inflammation.2 MyD88 expression in myeloid cells can rescue the intestinal injury induced by dextran sulfate sodium(DSS)in murine ***,the mechanism whereby MyD88 works in myeloid cells and influence the progress of the disease remain *** this study,we used DSS induced colitis mouse model and found Lysm-cre-MyD88fl/fl(MyD88ΔМΦ)mice were more susceptible to *** deficiency of MyD88 leads to up-regulation of S100A8,which activate NLRP3 inflammasome and their associated pyroptosis in intestinal epithelial cells in a RAGE dependent ***,MyD88 signaling in macrophages,which is necessary to maintain intestinal homeostasis,crucially prevents the development of *** results may shed new lights on potential targets that can be applied in colitis therapy.

关键词： NLRP3 colitis homeostasis

Construction and characterization of a recombinant fowlpox virus containing HIV-1 multi-epitope-p24 chimeric gene in mice

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Science China(Life Sciences) 2007年第2期50卷 212-220页

作者： zhang lishu JIN NingYi SONG YingJin WANG Hong MA HeWen LI ZiJian JIANG WenZheng The 11th Institute Academy of Military Medical SciencesChangchun 130062China College of Agricultural and Biological Engineer Tianjin UniversityTianjin 300072China College of Life Science Jinan UniversityGuangzhou 510632China Department of Biochemistry and Molecular Biology Oklahoma State UniversityStillwater 74078USA Institute of Vascular Medicine Third Affiliated Hospital of Peking UniversityBeijing 100083China School of Life Science East China Normal UniversityShanghai 200026China

The epidemic of HIV/AIDS is sweeping across the world. It is of great importance to figure out new ways to curb this disease. Epitope-based vaccine is one of these solutions. In this study, a chimeric gene was obtained by combination of a designed HIV-1 multi-epitope gene (MEG) and HIV-1 p24 gene. A re- combinant plasmid pUTA2-MEGp24 was then constructed by inserting MEGp24 gene into the down- stream of the promoter (ATI-P7.5×20) of fowlpox virus (FPV) transfer vector pUTA2. The recombinant plasmid and wild-type FPV 282E4 strain were then co-transfected into CEF cells and homologous re- combination occurred. A recombinant virus expressing HIV-1 protein MEGp24 was screened by ge- nome PCR and Western blot assay. Large scale preparation and purification of the recombinant fowl- pox virus (rFPV) were then carried out. BALB/c mice were immunized intramuscularly with the rFPV for three times on day 0, 14 and 42. Mice were executed and sampled one week after the third inoculation. Anti-HIV-1 antibody in serum and Th1 cytokines in the supernatant of cultured spleen cells were as- sayed by ELISA. The count of T lymphocyte subsets and the CTL activity of spleen lymphocytes were analyzed by flow cytometry and lactate dehydrogenase (LDH) release assay, respectively. The results showed that HIV-1 specific antibody in serum and increased T lymphocyte subsets (CD4+ T, CD8+ T) were detected in the immunization group. CTL target-killing activity and higher secretion of Th1 cyto- kines (IFN-γ and IL-2) of spleen lymphocytes stimulated by H-2d-restricted CTL peptide were observed in immunized mice. We concluded that the rFPV may induce HIV-1 specific immunity especially cellular immunity in mice.

关键词： HIV-1 multi-epitope recombinant fowlpox virus immune response

UHRF1/DNMT1—MZF1 axis-modulated intragenic site-specific CpGI methylation confers divergent expression and opposing functions of PRSS3 isoforms in lung cancer

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Acta Pharmaceutica Sinica B 2023年第5期13卷 2086-2106页

作者： Shuye Lin Hanli Xu Lin Qin Mengdi Pang Ziyu Wang Meng Gu lishu zhang Cong Zhao Xuefeng Hao Zhiyun zhang Weimin Ding Jianke Ren Jiaqiang Huang Cancer Research Center Beijing Chest HospitalCapital Medical UniversityBeijing Tuberculosis and Thoracic Tumor InstituteBeijing 101149China College of Life Sciences&Bioengineering Beijing Jiaotong UniversityBeijing 100044China Department of Endoscopic Diagnosis and Treatment Beijing Chest HospitalCapital Medical UniversityBeijing Tuberculosis and Thoracic Tumor InstituteBeijing 101149China CAS Key Laboratory of Computational Biology Shanghai Institute of Nutrition and HealthUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China

As confusion mounts over RNA isoforms involved in phenotypic plasticity,aberrant CpG methylation-mediated disruption of alternative splicing is increasingly recognized as a driver of intratumor heterogeneity(ITH).Protease serine 3(PRSS3),possessing four splice variants(PRSS3-SVs;PRSS3-V1—V4),is an indispensable trypsin that shows paradoxical effects on cancer ***,we found that PRSS3 transcripts and their isoforms were divergently expressed in lung cancer,exhibiting opposing functions and clinical outcomes,namely,oncogenic PRSS3-V1 and PRSS3-V2 versus tumorsuppressive PRSS3-V3,by targeting different downstream *** identified an intragenic CpG island(iCpGI)in *** of iCpGI was mediated by UHRF1/DNMT1 complex interference with the binding of myeloid zinc finger 1(MZF1)to regulate PRSS3 *** garlic-derived compound diallyl trisulfide cooperated with 5-aza-2'-deoxycytidine to exert antitumor effects in lung adenocarcinoma cells through site-specific iCpGI demethylation specifically allowing MZF1 to upregulate PRSS3-V3 *** silencing of PRSS3-V3 via i CpGI methylation(iCpGIm)in BALF and tumor tissues was associated with early clinical progression in patients with lung cancer but not in those with squamous cell carcinoma or inflammatory ***,UHRF1/DNMT1—MZF1 axismodulated site-specific iCpGIm regulates divergent expression of PRSS3-SVs,conferring nongenetic functional ITH,with implications for early detection of lung cancer and targeted therapies.

关键词： Lung cancer Intratumor heterogeneity Splice variants Intragenic CpG methylation Protease serine 3 Myeloid zinc finger 1 UHRF1/DNMT1 5-Aza-CdR

Immune responses of a designed HIV-1 DNA vaccine on rhesus monkeys

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Chinese Science Bulletin 2006年第13期51卷 1571-1577页

作者： zhang lishu JIN Ningyi SONG Yingjin SUN Yansong WANG Hong ZHAN Dawei MA Hewen SHANG Yupu JIN Hongtao HONG Baoqing LI Chang The 11th Institute Academy of Military Medical Sciences Changchun 130062 China College of Agricultural and Biological Engineer Tianjin University Tianjin 300072 China Laboratory Animal Center Academy of Military Medical Sciences Beijing 100071 China College of Life Science Jinan University Guangzhou 510632 China Agricultural Sciences Center Jilin University Changchun 130062 China

An effective HIV-1 vaccine will be the ultimate solution for the prevention of HIV/AIDS, though HAART plays important roles in treating the disease. In this study, a large-scale recombinant DNA plasmid containing a designed HIV-1 multi-epitope- p24 chimeric gene was prepared and purified. Rhesus monkeys were then inoculated muscularly with the plasmid for four times in week 0, 4, 8 and 18. Whole blood was collected two weeks after the third and fourth inoculation, followed by serum and pe- ripheral blood mononuclear cell (PBMC) separation. The CTL activity and proliferation of PBMCs stimu- lated by macaque MHC-I-restricted HIV-1 CTL epi- tope peptide were analyzed by MTT and LDH release assay, respectively. Th1 cytokines in supernatant of cultured PBMC stimulated by HIV-1 CTL epitope peptide and anti-HIV-1 antibody in serum were as- sayed by ELISA. The results showed that increased CTL target-killing activity, higher secretion of Th1 cytokines (IFN-γ and IL-2) and promoted proliferative reaction of monkey PBMCs stimulated by HIV-1 CTL epitope peptide were detected in the immunization group inoculated by the recombinant DNA vaccine for three times, which were further enhanced by the fourth inoculation. At the same time, HIV-1 specific antibody in serum of immunized monkeys was higher than that in controls. We concluded that the designedHIV-1 DNA vaccine may induce HIV-1 specific cellular and humoral immunity on monkeys.

关键词： HIV-1 DNA疫苗免疫响应猕猴

A genome wide association study between copy number variation (CNV) and human height in Chinese population

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Journal of Genetics and Genomics 2010年第12期37卷 779-785页

作者： Xi Li Lijun Tan Xiaogang Liu Shufeng Lei Tielin Yang Xiangding Chen Fang zhang Yue Fang Yan Guo Liang zhang Han Yan Feng Pan Zhixin zhang Yumei Peng Qi Zhou Lina He Xuezhen Zhu Jing Cheng lishu zhang Yaozhong Liu Qing Tian Hongwen Deng Laboratory of Molecular and Statistical Genetics College of Life Sciences Hunan Normal University Changsha 410081 China The Key Laboratory of Biomedical Information Engineering of Ministry of Education and Institute of Molecular Genetics School of Life Science and Technology Xi'an Jiaotong University Xi'an 710049 China National Engineering Research Center for Beijing Biochip Technology Beijing 102206 China Department of Basic Medical Science and Orthopedic Surgery School of Medicine University of Missouri - Kansas City Kansas City MO 64108 USA College of Life Sciences and Bioengineering Beijing Jiaotong University Beijing 100044 China

Copy number variation （CNV） is a type of genetic variation which may have important roles in phenotypic variability and disease susceptibility. To hunt for genetic variants underlying human height variation, we performed a genome wide CNV association study for human height in 618 Chinese unrelated subjects using Affymetrix 500K array set. After adjusting for age and sex, we found that four CNVs at 6p21.3, 8p23.3-23.2, 9p23 and 16p12.1 were associated with human height （with borderline significant p value： 0.013, 0.011, 0.024, 0.049; respectively）. However, after multiple tests correction, none of them was associated with human height. We observed that the gain of copy number （more than 2 copies） at 8p23.3-23.2 was associated with lower height （normal copy number vs. gain of copy number： 161.2 cm vs. 153.7 cm, p = 0.011）, which accounted for 0.9% of height variation. Loss of copy number （less than 2 copies） at 6p21.3 was associated with 0.8% lower height （loss of copy number vs. normal copy number： 154.5 cm vs. 161.1 cm, p = 0.013）. Since no important genes influencing height located in CNVs at loci of 8p23.3-23.2 and 6p21.3, the two CNVs may cause the structural rear- rangements of neighbored important candidate genes, thus regulates the variation of height. Our results expand our knowledge of the genetic factors underlying height variation and the biological regulation of human height.

关键词： CNV human height GWAS Affymetrix 500K array

A real-time AI-assisted seismic monitoring system based on new nodal stations with 4G telemetry and its application in the Yangbi M_(S) 6.4 aftershock monitoring in southwest China

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Earthquake Research Advances 2022年第2期2卷 3-10页

作者： Junlun Li Huajian Yao Baoshan Wang Yang Yang Xin Hu lishu zhang Beng Ye Jun Yang Xiaobin Li Feng Liu Guoyi Chen Chang Guo Wen Yang Laboratory of Seismology and Physics of Earth's Interior School of Earth and Space ScienceUniversity of Science and Technology of China96 Jinzhai RoadHefeiAnhui230026China Mengcheng National Geophysical Observatory University of Science and Technology of ChinaHefeiAnhui230026China Center for Advanced Geophysical Sensing and Intelligent Exploration Institute of Advanced TechnologyUniversity of Science and Technology of China96 Jinzhai RoadHefeiAnhui230026China Hefei Guowei Electronics Co.Ltd. 800 Wangjiang West RoadHefeiAnhui230088China Yunnan Earthquake Agency KunmingYunnan650224China

A rapidly deployable dense seismic monitoring system which is capable of transmitting acquired data in real time and analyzing data automatically is crucial in seismic hazard mitigation after a major ***,it is rather difficult for current seismic nodal stations to transmit data in real time for an extended period of time,and it usually takes a great amount of time to process the acquired data *** monitor earthquakes in real time flexibly,we develop a mobile integrated seismic monitoring system consisting of newly developed nodal units with 4G telemetry and a real-time AI-assisted automatic data processing *** integrated system is convenient for deployment and has been successfully applied in monitoring the aftershocks of the Yangbi M_(S) 6.4 earthquake occurred on May 21,2021 in Yangbi County,Dali,Yunnan in southwest *** acquired seismic data are transmitted almost in real time through the 4G cellular network,and then processed automat-ically for event detection,positioning,magnitude calculation and source mechanism *** tens of seconds to a couple of minutes at most,the final seismic attributes can be presented remotely to the end users through the integrated *** May 27 to June 17,the real-time system has detected and located 7905 aftershocks in the Yangbi area before the internal batteries exhausted,far more than the catalog provided by China Earthquake Networks Center using the regional permanent *** initial application of this inte-grated real-time monitoring system is promising,and we anticipate the advent of a new era for Real-time Intelligent Array Seismology(RIAS),for better monitoring and understanding the subsurface dynamic pro-cesses caused by Earth's internal forces as well as anthropogenic activities.

关键词： Seismic dense array 4G data transmission Real-time earthquake monitoring Machine-learning assisted processing Real-time intelligent array seismology

Studies on the isolation of Echinococcus granulosus cyst fluid antigen by D-type acrylamide gel large column electrophoresis.

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中国寄生虫学与寄生虫病杂志 1988年第S1期 186-187页

作者： Xue Haichou, Qiu lishu, zhang Yonghong, Zhu Zhenxia: Institute of Parasitic Difeases, Chinese Academy of Preventive Medicine

Cyst fluid antigen of Echinococcus granulosus was isolated by acrylamide gel electrophoresisusing D-type large column-a new product of the 1980’s.7 antigens with different composition were

关键词： Studies on the isolation of Echinococcus granulosus cyst fluid antigen by D-type acrylamide gel large column electrophoresis type

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西部大开发战略实施效果综合评价

科技进步与对策 2009年第10期26卷 46-50页

作者：彭晖张丽淑西安交通大学经济与金融学院陕西西安710061

以"国家西部大开发总体战略规划"为依据,建立了西部大开发战略实施情况评价指标体系。分别采用单指标历史标准的方法,以及多指标综合的主成分分析方法对战略实施效果进行了评价。不仅从纵向分析了大开发战略实施前后西部地区的变化,而... 详细信息

以"国家西部大开发总体战略规划"为依据,建立了西部大开发战略实施情况评价指标体系。分别采用单指标历史标准的方法,以及多指标综合的主成分分析方法对战略实施效果进行了评价。不仅从纵向分析了大开发战略实施前后西部地区的变化,而且对西部地区十二省份进行了横向比较,全面论证了西部大开发战略实施7年以来西部地区的变化过程和现状,以期为西部大开发战略的继续实施提供参考。

关键词：西部大开发战略效果综合评价主成分分析