Immune responses of a designed HIV-1 DNA vaccine on rhesus monkeys

作     者：ZHANG Lishu JIN Ningyi SONG Yingjin SUN Yansong WANG Hong ZHAN Dawei MA Hewen SHANG Yupu JIN Hongtao HONG Baoqing LI Chang 

作者机构：The 11th Institute Academy of Military Medical Sciences Changchun 130062 China College of Agricultural and Biological Engineer Tianjin University Tianjin 300072 China Laboratory Animal Center Academy of Military Medical Sciences Beijing 100071 China College of Life Science Jinan University Guangzhou 510632 China Agricultural Sciences Center Jilin University Changchun 130062 China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2006年第51卷第13期

页      面：1571-1577页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：National 863 Foundation Project  (2003AA219051) 

主　　题：HIV-1 DNA疫苗 免疫响应 猕猴 

摘      要：An effective HIV-1 vaccine will be the ultimate solution for the prevention of HIV/AIDS, though HAART plays important roles in treating the disease. In this study, a large-scale recombinant DNA plasmid containing a designed HIV-1 multi-epitope- p24 chimeric gene was prepared and purified. Rhesus monkeys were then inoculated muscularly with the plasmid for four times in week 0, 4, 8 and 18. Whole blood was collected two weeks after the third and fourth inoculation, followed by serum and pe- ripheral blood mononuclear cell (PBMC) separation. The CTL activity and proliferation of PBMCs stimu- lated by macaque MHC-I-restricted HIV-1 CTL epi- tope peptide were analyzed by MTT and LDH release assay, respectively. Th1 cytokines in supernatant of cultured PBMC stimulated by HIV-1 CTL epitope peptide and anti-HIV-1 antibody in serum were as- sayed by ELISA. The results showed that increased CTL target-killing activity, higher secretion of Th1 cytokines (IFN-γ and IL-2) and promoted proliferative reaction of monkey PBMCs stimulated by HIV-1 CTL epitope peptide were detected in the immunization group inoculated by the recombinant DNA vaccine for three times, which were further enhanced by the fourth inoculation. At the same time, HIV-1 specific antibody in serum of immunized monkeys was higher than that in controls. We concluded that the designedHIV-1 DNA vaccine may induce HIV-1 specific cellular and humoral immunity on monkeys.