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Comprehensive analysis of the gut microbiome and posttranslational modifications elucidates the route involved in microbiota-host interactions

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Zoological Research 2024年第1期45卷 95-107页

作者： Hai-yang Wang Lan-xiang liu Xue-Yi Chen yang-Dong zhang wen-Xia li wen-wen li lian Wang Xiao-Long Mo Hong Wei Ping Ji Peng Xie NHC Key Laboratory of Diagnosis and Treatment on Brain Functional Diseases First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China College of Stomatology and Affiliated Stomatological Hospital of Chongqing Medical University Chongqing 401147China Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences Chongqing 401147China Department of Neurology Yongchuan Hospital of Chongqing Medical UniversityChongqing 402160China Department of Pathology Faculty of Basic MedicineChongqing Medical UniversityChongqing 400016China Department of Neurology First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Yu-Yue Pathology Scientific Research Center Chongqing 401329China

The gut microbiome interacts with the host to maintain body homeostasis,with gut microbial dysbiosis implicated in many ***,the underlying mechanisms of gut microbe regulation of host behavior and brain functions remain *** study aimed to elucidate the influence of gut microbiota on brain functions via post-translational modification mechanisms in the presence or absence of bacteria without any *** conducted succinylome analysis of hippocampal proteins in germ-free(GF)and specific pathogen-free(SPF)mice and metagenomic analysis of feces from SPF *** results were integrated with previously reported hippocampal acetylome and phosphorylome data from the same batch of *** bioinformatics analyses revealed 584 succinylation sites on 455 proteins,including 54 up-regulated succinylation sites on 91 proteins and 99 down-regulated sites on 51 proteins in the GF mice compared to the SPF *** constructed a panoramic map of gut microbiota-regulated succinylation,acetylation,and phosphorylation,and identified cross-talk and relative independence between the different types of post-translational modifications in modulating complicated intracellular *** correlation analysis indicated that 13 taxa,predominantly belonging to the Bacteroidetes phylum,were correlated with the biological functions of post-translational *** correlations between these taxa and succinylation and negative correlations between these taxa and acetylation were identified in the modulation of intracellular *** study highlights the hippocampal physiological changes induced by the absence of gut microbiota,and proteomic quantification of succinylation,phosphorylation,and acetylation,contributing to our understanding of the role of the gut microbiome in brain function and behavioral phenotypes.

关键词： Gut microbiota Hippocampal protein Post-translational modifications Succinylation Acetylation Phosphorylation

Efficient and precise genomic deletion in rice using enhanced prime editing

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aBIOTECH 2024年第2期5卷 214-218页

作者： Mengyuan liu xiang zhang wen Xu Guiting Kang Ya liu Xinxiang liu wen Ren Jiuran Zhao Jinxiao yang Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China

Efficient and precise genomic deletion shows promise for investigating the function of proteins in plant research and enhancing agricultural *** this study,we tested the PRIME-Del(PDel)strategy using a pair of prime editing guide RNAs(pegRNAs)that targeted opposite DNA strands and achieved an average deletion efficiency of 55.8%for 60 bp fragment deletions at six endogenous ***,as high as 84.2%precise deletion efficiency was obtained for a 2000 bp deletion at the OsGS1 site in transgenic rice *** add the bases that were unintentionally deleted between the two nicking sequences,we used the PDel/Syn strategy,which introduced multiple synonymous base mutations in the region that had to be patched in the RT *** PDel/Syn strategy achieved an average of 58.1%deletion efficiency at six endogenous targets,which was higher than the PDel *** strategies presented in this study contribute to achieving more accurate and flexible deletions in transgenic rice plants.

关键词： Prime editing Precise genomic deletion CRISPR Cas9

The energy response of LaBr_(3)(Ce),LaBr_(3)(Ce,Sr),and NaI(Tl)crystals for GECAM

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Nuclear Science and Techniques 2024年第2期35卷 110-122页

作者： Pei-Yi Feng Xi-Lei Sun Zheng-Hua An Yong Deng Cheng-Er Wang Huang Jiang Jun-Jie li da-li zhang Xin-Qiao li Shao-lin Xiong Chao Zheng Ke Gong Sheng yang Xiao-Jing liu Min Gao xiang-yang wen Ya-Qing liu Yan-Bing Xu Xiao-Yun Zhao Jia-Cong liu Fan zhang Hong Lu Particle and Astrophysics Center Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Chinese Academy of SciencesBeijing 100049China Experimental Physics Center Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China State Key Laboratory of Particle Detection and Electronics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China School of Nuclear Science and Technology University of South ChinaHengyang 421001China National Engineering Research Center for Rare Earth Grirem Advanced Materials Co.Ltd.and General Research Institute for Nonferrous MetalsBeijing 100088China

The GECAM series of satellites utilizes LaBr_(3)(Ce),LaBr_(3)(Ce,Sr),and NaI(Tl)crystals as sensitive materials for gamma-ray detectors(GRDs).To investigate the nonlinearity in the detection of low-energy gamma rays and address the errors in the calibration of the E-C relationship,comprehensive tests and comparative studies of the three aforementioned crystals were conducted using Compton electrons,radioactive sources,and mono-energetic *** nonlinearity test results of the Compton electrons and X-rays demonstrated substantial differences,with all three crystals presenting a higher nonlinearity for X/-rays than for Compton *** the LaBr_(3)(Ce)and LaBr_(3)(Ce,Sr)crystals having higher absolute light yields,they exhibited a noticeable nonlinear decrease in the light yield,especially at energies below 400 *** NaI(Tl)crystal demonstrated an"excess"light output in the 6-200 keV range,reaching a maximum"excess"of 9.2%at 30 keV in the X-ray testing and up to 15.5%at 14 keV during Compton electron testing,indicating a significant advantage in the detection of low-energy gamma ***,we explored the underlying causes of the observed nonlinearity in these *** study not only elucidates the detector responses of GECAM,but also initiates a comprehensive investigation of the nonlinearity of domestically produced lanthanum bromide and sodium iodide crystals.

关键词： LaBr_(3)(Ce)detector LaBr_(3)(Ce,Sr)detector NaI(Tl)detector GECAM Energy response light yield nonlinearity

Clinical Analysis and Mental Health Survey of Hemophilia Carriers:a Cross-sectional Study

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Current Medical Science 2024年第2期44卷 435-440页

作者： wen WANG li-juan JIANG Dong-yan CUI Ai zhang Xiong WANG Ai-guo liu qun HU Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Laboratory Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Objective:Hemophilia carriers(HCs),who are heterozygous for mutations in the clotting factor VIII/clotting factor IX gene(F8 or F9),may have a wide range of clotting factor levels,from very low,similar to afflicted males,to the upper limit of normal,and may experience mental health *** purpose of this study was to provide genetic information on mothers of hemophilia patients and to understand the clotting factor activity and phenotype of ***,we aimed to investigate the mental health status of HCs in ***:A total of 127 hemophilia mothers,including 93 hemophilia A(HA)mothers and 34 hemophilia B(HB)mothers,were enrolled in this *** distance PCR,multiplex PCR,and Sanger sequencing were used to analyze mutations in F8 or *** factor activity was detected by a one-stage clotting *** Symptom Checklist 90(SCL-90,China/Mandarin version)was given to HCs at the same time to assess their mental ***:A total of 90.6%of hemophilia mothers were diagnosed genetically as carriers,with inversion in intron 22 and missense mutations being the most common mutation types in HA and HB carriers,*** median clotting factor level in carriers was 0.74 IU/mL(ranging from 0.09 to 1.74 IU/mL)compared with 1.49 IU/mL(ranging from 0.93 to 1.89 IU/mL)in noncarriers,of which 14.3%of HCs had clotting factor levels of 0.40 IU/mL or below.A total of 53.8%(7/13)of HA carriers with low clotting factor levels(less than 0.50 IU/mL)had a history of bleeding,while none of the HB carriers displayed a bleeding *** total mean score and the global severity index of the SCL-90 for surveyed HCs were 171.00(±60.37)and 1.78(±0.59),respectively.A total of 67.7%of the respondents had psychological symptoms,with obsessive-compulsive disorder being the most prevalent and *** pooled estimates of all nine factors were significantly higher than those in the general population(P<0.05).Conclusions:The detection rate of gene mutations

关键词： hemophilia A hemophilia B mutation carrier mental health

Novel miR-490-3p/hnRNPA1-b/PKM2 axis mediates the Warburg effect and proliferation of colon cancer cells via the PI3K/AKT pathway

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 2038-2059页

作者： xiang-Hui Wan Guo-Bing Jin qun yang Ji-Long Hu Zhi-liang liu Jun Rao Can wen Peng-ling li Xi-Mei yang Bo Huang Xiao-Zhong Wang Jiangxi Medical College Nanchang UniversityNanchang 330029Jiangxi ProvinceChina Department of Clinical Laboratory Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Jiangxi Key Laboratory of Translational Research for Cancer Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Department of Abdominal Surgery Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Department of Pathology Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Science and Education Section Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Department of Clinical Laboratory Jiangxi Children’s HospitalNanchang 330006Jiangxi ProvinceChina Department of Clinical Laboratory The Second Affiliated Hospital of Nanchang UniversityNanchang 330006Jiangxi ProvinceChina Jiangxi Province Key Laboratory of Laboratory Medicine The Second Affiliated Hospital of Nanchang UniversityNanchang 330006Jiangxi ProvinceChina

BACKGROUND Heterogeneous ribonucleoprotein A1(hnRNPA1)has been reported to enhance the Warburg effect and promote colon cancer(CC)cell proliferation,but the role and mechanism of the miR-490-3p/hnRNPA1-b/PKM2 axis in CC have not yet been *** To investigate the role and mechanism of a novel miR-490-3p/hnRNPA1-b/PKM2 axis in enhancing the Warburg effect and promoting CC cell proliferation through the PI3K/AKT *** Paraffin-embedded pathological sections from 220 CC patients were collected and subjected to immunohistochemical analysis to determine the expression of *** relationship between the expression values and the clinicopathological features of the patients was *** in mRNA expression were analyzed using quantitative real-time polymerase chain reaction,while differences in protein expression were analyzed using western *** proliferation was evaluated using the cell counting kit-8 and 5-ethynyl-2’-deoxyuridine assays,and cell cycle and apoptosis were detected using flow cytometric *** targeted binding of miR-490-3p to hnRNPA1-b was validated using a dual luciferase reporter *** Warburg effect was evaluated by glucose uptake and lactic acid production *** The expression of hnRNPA1-b was significantly increased in CC tissues and cells compared to normal controls(Plinicopathologic characterization revealed a significant correlation between hnRNPA1-b expression and clinical stage as well as T ***1-b was found to enhance the Warburg effect through the PI3K/AKT pathway,thereby promoting proliferation of HCT116 and SW620 ***,the proliferation of HCT116 and SW620 cells was inhibited when miR-490-3p targeted and bound to hnRNPA1-b,effectively blocking the Warburg ***

关键词： Heterogeneous ribonucleoprotein A1-b MiR-490-3p Colon cancer Alternative splicing Warburg effect

Observation of spectral lines in the exceptional GRB 221009A

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Science China(Physics,Mechanics & Astronomy) 2024年第8期67卷 187-206页

作者： Yan-Qiu zhang Shao-lin Xiong Ji-rong Mao Shuang-Nan zhang Wang-Chen Xue Chao Zheng Jia-Cong liu Zhen zhang Xi-Lu Wang Ming-Yu Ge Shu-Xu Yi li-Ming Song Zheng-Hua An Ce Cai Xin-Qiao li wen-Xi Peng wen-Jun Tan Chen-Wei Wang xiang-yang wen Yue Wang Shuo Xiao Fan zhang Peng zhang Shi-Jie Zheng Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Beijing 100049China Yunnan Observatories Chinese Academy of SciencesKunming 650011China Center for Astronomical Mega-Science Chinese Academy of SciencesBeijing 100012China Key Laboratory for the Structure and Evolution of Celestial Objects Chinese Academy of SciencesKunming 650216China College of Physics and Hebei Key Laboratory of Photophysics Research and Application Hebei Normal UniversityShijiazhuang 050024China Guizhou Provincial Key Laboratory of Radio Astronomy and data Processing Guizhou Normal UniversityGuiyang 550001China College of Electronic and Information Engineering Tongji UniversityShanghai 201804China

As the brightest gamma-ray burst ever observed,GRB 221009A provided a precious opportunity to explore spectral line *** this article,we performed a comprehensive spectroscopy analysis of GRB 221009A jointly with GECAM-C and Fermi/GBM data to search for emission and absorption *** the first time we investigated the line feature throughout this GRB including the most bright part where many instruments suffered problems,and identified prominent emission lines in multiple time *** central energy of the Gaussian emission line evolves from about 37 to 6 MeV,with a nearly constant ratio(about 10%)between the line width and central ***,we find that both the central energy and the energy flux of the emission line evolve with time as a power law decay with power law index of–1 and–2,*** suggest that the observed emission lines most likely origin from the blue-shifted electron positron pair annihilation 511 ke V *** find that a standard high latitude emission scenario cannot fully interpret the observation,thus we propose that the emission line comes from some dense clumps with electron positron pairs traveling together with the *** this scenario,we can use the emission line to directly,for the first time,measure the bulk Lorentz factor of the jet(Γ)and reveal its time evolution(i.e.,Γ~t^(-1))during the prompt ***,we find that the flux of the annihilation line in the co-moving frame keeps *** discoveries of the spectral line features shed new and important lights on the physics of GRB and relativistic jet.

关键词： GRB GRB 221009A emission line

Diagnostic and prognostic role of liNC01767 in hepatocellular carcinoma

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World Journal of Hepatology 2024年第6期16卷 932-950页

作者： li zhang Tong-Xing Cui xiang-Zhi li Chong liu wen-Qin Wang Department of Thyroid and Breast Surgery The Affiliated People Hospital of Second Medical UniversityWeifang 266010Shandong ProvinceChina Department of General Surgery Qingdao Municipal Hospital GroupQingdao 266237Shandong ProvinceChina School of life Sciences Shandong University(Qingdao)Qingdao 26637Shandong ProvinceChina School of Medicine Tsinghua UniversityBeijing 100084China

BACKGROUND Hepatocellular carcinoma(HCC)is a primary contributor to cancer-related mortality on a global ***,the underlying molecular mechanisms are still poorly *** noncoding RNAs are emerging markers for HCC diagnosis,prognosis,and therapeutic *** study of liNC01767 in HCC was *** To conduct a multi-omics analysis to explore the roles of liNC01767 in HCC for the first *** DESeq2 Package was used to analyze different gene *** operating characteristic curves assessed the diagnostic ***-Meier univariate and Cox multivariate analyses were used to perform survival *** least absolute shrinkage and selection operator(LASSO)-Cox was used to identify the prediction *** to the validation of liNC01767 expression in HCC fresh frozen tissues through quantitative real time polymerase chain reaction,next generation sequencing was performed following liNC01767 over expression(GSE243371),and Gene Ontology/Kyoto Encyclopedia of Genes and Genomes/Gene Set Enrichment Analysis/ingenuity pathway analysis was carried *** vitro experiment in Huh7 cell was carried *** liNC01767 was down-regulated in HCC with a log fold change=1.575 and was positively correlated with the cancer ***01767 was a good diagnostic marker with area under the curve(AUC)[0.801,95% confidence interval(CI):0.751-0.852,P=0.0106]and an independent predictor for overall survival(OS)with hazard ratio=1.899(95%CI:1.01-3.58,P=0.048).liNC01767 nomogram model showed a satisfied *** top-ranked regulatory network analysis of liNC01767 showed the regulation of genes participating various *** regression identified the 9-genes model showing a more satisfied performance than 5-genes model to predict the OS with AUC>***01767 was down-expressed obviously in tumor than para-tumor tissues in our cohort as well as in cancer cell line;the over expression of liNC01767 inhibit cell prolifer

关键词： Hepatocellular carcinoma liNC01767 Multi-omics analysis GSE243371 Cell proliferation Clone formation

Effect of slow shot speed on externally solidified crystal,porosity and tensile property in a newly developed high-pressure die-cast Al-Si alloy

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China Foundry 2024年第1期21卷 11-19页

作者： wen-ning liu Wei zhang Peng-yue Wang Yi-xian liu xiang-yi Jiao Ao-xiang Wan Cheng-gang Wang Guo-dong Tong Shou-mei Xiong School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China Key Laboratory for Advanced Materials Processing Technology Tsinghua UniversityBeijing 100084Beijing China FAW Group Co. Ltd.Changchun 130013China China FAW Foundry Co. Ltd.Changchun 130013China

The effect of slow shot speed on externally solidified crystal(ESC),porosity and tensile property in a newly developed high-pressure die-cast Al-Si alloy was investigated by optical microscopy(OM),scanning electron microscopy(SEM)and laboratory computed tomography(CT).Results showed that the newly developed AlSi9MnMoV alloy exhibited improved mechanical properties when compared to the AlSi10MnMg *** AlSi9MnMoV alloy,which was designed with trace multicomponent additions,displays a notable grain refining effect in comparison to the AlSi10MnMg *** elements Ti,Zr,V,Nb,B promote heterogeneous nucleation and reduce the grain size of primaryα-*** a lower slow shot speed,the large ESCs are easier to form and gather,developing into the dendrite net and *** an increase in slow shot speed,the size and number of ESCs and porosities significantly *** addition,the distribution of ESCs is more dispersed and the net-shrinkage *** tensile property is greatly improved by adopting a higher slow shot *** ultimate tensile strength is enhanced from 260.31 MPa to 290.31 MPa(increased by 11.52%),and the elongation is enhanced from 3.72%to 6.34%(increased by 70.52%).

关键词： hypoeutectic Al-Si alloy high pressure die casting porosity externally solidified crystal tensile property

Perspective of Calcium Imaging Technology Applied to Acupuncture Research

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Chinese Journal of Integrative Medicine 2024年第1期30卷 3-9页

作者： li Sha liu Yun zhang Nan li Wang XU wen-jie XU Yi-qian CHEN Yi-yuan CUI xiang ZHU Bing GAO Xin-yan Institute of Acupuncture and Moxibustion China Academy of Chinese Medical SciencesBeijing(100700)China

Acupuncture, a therapeutic treatment defined as the insertion of needles into the body at specific points(ie, acupoints), has growing in popularity world-wide to treat various diseases effectively, especially acute and chronic pain. In parallel, interest in the physiological mechanisms underlying acupuncture analgesia, particularly the neural mechanisms have been increasing. Over the past decades, our understanding of how the central nervous system and peripheral nervous system process signals induced by acupuncture has developed rapidly by using electrophysiological methods. However, with the development of neuroscience, electrophysiology is being challenged by calcium imaging in view field, neuron population and visualization in vivo. Owing to the outstanding spatial resolution, the novel imaging approaches provide opportunities to enrich our knowledge about the neurophysiological mechanisms of acupuncture analgesia at subcellular, cellular, and circuit levels in combination with new labeling, genetic and circuit tracing techniques. Therefore, this review will introduce the principle and the method of calcium imaging applied to acupuncture research. We will also review the current findings in pain research using calcium imaging from in vitro to in vivo experiments and discuss the potential methodological considerations in studying acupuncture analgesia.

关键词： acupuncture research calcium imaging optical imaging nervous system