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Effects of 105 traditional Chinese medicines on the detection ofβ-agonists in medicine extracts and swine urine based on colloidal gold immunochromatographic assay

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Journal of Integrative Agriculture 2021年第6期20卷 1626-1635页

作者： WANG Zi-lin FENG Ke-ying GE Xiu-feng MAI jia-cheng WANG Han-chuan LIU Wen-zi zhang jia-hui SHEN Xiang-guang Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation South China Agricultural UniversityGuangzhou 510642P.R.China Animal and Poultry Products Quality Control Inspection and Testing CenterMinistry of Agriculture and Rural AffairsGuangzhou 510642P.R.China

Colloidal gold immunochromatographic assay(CGIA)is commonly used for the on-site detection ofβ-agonists that are sometimes used illegally as feed additives in swine ***,few studies have evaluated the causes of false-positive results that sometimes occur when applying CGIA in agricultural *** this study,we investigated if this false-positive phenomenon is related to the addition of certain traditional Chinese medicines(TCMs)to swine *** established and verified an extraction method for TCMs,and then applied CGIA to detectβ-agonists in the extracts of 105 TCMs and in the urine of swine dosed with TCMs,*** chromatography-tandem mass spectrometry was used to validate the results of the urine samples tested positive forβ-agonists using *** results were also verified using TCMs and colloidal gold test strips produced by different *** extracts of Citri Reticulatae Pericarpium Viride,Citri Reticulatae Pericarpium,Magnoliae Officinalis Cortex,Chaenomelis Fructus,and Rhodiolae Crenulatae Radix Et Rhizoma were tested positive forβ-***,the addition of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium to swine feed resulted in false-positive results forβ-agonists in swine *** results provide a new way to explain false-positive CGIA results and provide valuable information for livestock feeding programs.

关键词： colloidal gold immunochromatographic assay false positive traditional Chinese medicine β-agonists swine urine

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蛋白质计算中的机器学习

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物理学报 2024年第6期73卷 95-107页

作者：张嘉晖中国科学技术大学生命科学学院合肥230027

蛋白质计算一直以来都是科学领域中的重要课题,而近年来其与机器学习的结合,更是极大地推进了相关学科的发展.本综述主要讨论了机器学习在四个重要的蛋白质计算领域内的研究进展,这四个领域包括:分子动力学模拟、结构预测、性质预测和... 详细信息

蛋白质计算一直以来都是科学领域中的重要课题,而近年来其与机器学习的结合,更是极大地推进了相关学科的发展.本综述主要讨论了机器学习在四个重要的蛋白质计算领域内的研究进展,这四个领域包括:分子动力学模拟、结构预测、性质预测和分子设计.分子动力学模拟依赖于力场参数,准确的力场参数是分子动力学模拟的必需品,而机器学习可以帮助研究者得到更加准确的力场参数.在分子动力学模拟中,机器学习也可以从复杂的体系中以较小的代价计算出所需求解的自由能.结构预测一般是给定蛋白质序列预测其结构.结构预测复杂度高、数据量大,而这恰恰是机器学习所擅长的.在机器学习的协助下,近年来科研人员已经在单个蛋白质三维结构预测上取得了不错的成果.性质预测则是指通过给定的已知蛋白质信息,推断其可能拥有的性质,这对于蛋白质的研究也是至关重要的.更具挑战性的是分子设计,虽然近年来机器学习在蛋白质设计上取得突破,但这一领域还有很大空间值得探索.本综述将针对以上四点分别展开论述,并对蛋白质计算中的机器学习研究进行展望.

关键词：蛋白质机器学习分子动力学模拟结构预测性质预测分子设计

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Expression and Purification of Goose HSP70 and Compound Formation with Virus Polypeptide

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Agricultural Sciences in China 2008年第2期7卷 239-247页

作者： WANG Bao-wei WU Xiao-ping zhang Xu-hui jia Xiao-hui zhang Ming-ai LONG Fang-yu YANG Zhi-gang WANG Lei Water Poultry Graduate School Laiyang Agricultural University Qingdao 266109 P.R.China

The experiment was conducted to study the specific expression of HSP70 caused by heat shock, HSP70 purification and the characteristics of coalescence with antigenic peptide in the formation of the complex. Sixty healthy 6-week-old male Wulong geese were selected and randomly divided into three groups. The control group was slaughtered without heat treatment. Treatment group 1 was shocked with an acute heat treatment at （42 ± 1）℃ for 5 h before they were immediately slaughtered. Treatment group 2 was kept for 12 h after the heat treatment under normal conditions in order to recover and was then slaughtered. Cardiac tissue was taken in order to make paraffin sections for the immunohistochemistry experiment and the liver tissue was used to purify HSP70. The geese heart HSP70 expression differences in the three groups were determined and at the same time the experiments of HSP70 purification and appraisal in the liver tissue were carried on. HSP70 purification and synthesis of HBV PreS1 multi-peptides unified the complex, which was determined by bi-specific antibody enzyme-linked immune sandwich assay. The results indicated that widespread HSP70 positive pellets in the cardiac muscle were found under hot shock conditions. HSP70 expression in the treatment group 1 was centered in the karyotheca and its periphery, but in treatment group 2, it was centered in the surrounding cell membrane. The HSP70 purification could be obtained through two sets of purification plans; both the synthesis peptide and the HSP70 purification form the complex under certain conditions. The double antibody sandwich ELISA technique was applied to detect if the complex had been formed. Positive results showed that the complex was formed. The specific expression of HSP70 under heat shock shifted with time, suggesting that HSP70 possibly had some function in cell protection. High-purity HSP70 protein can be obtained under low-pressure chromatography conditions, and in comparison with each other, it

关键词： heat shock goose HSP70 expression purification appraisal viral multi-peptides complex formation

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Classic herb pair sculellaria barbata and hedyotis diffusa for breast cancer: potential mechanism with method of network pharmacology

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中国药理学与毒理学杂志 2019年第10期 784-785页

作者： MA Ting-ting zhang Gan-lin DAI Cun-fang zhang Bo-ran LI Bing-xue zhang jia-hui WANG Xiao-min Oncology Department Beijing Hospital of Traditional Chinese Medicine Capital Medical University Beijing University of Chinese Medicine

OBJECTIVE To explore potential mechanisms of classic herb pair sculellaria barbata and hedyotisdiffusa（SH）, which are prescribed by many famous Chinese oncologists and proved effective curative effects for treatment of breast cancer. METHODS （1） SH lyophilized powder based on decoction was made for experiments and active ingredients in SH lyophilized powder were identified by high performance liquid chromatography（HPLC）.（2） Both human-derived and mouse-derived breast cancer cells including 231, MCF7 and 4T1 were used to evaluate efficacy of SH. Cell proliferation test and cell scratch test were respectively used to assess effects of SH on breast cancer cells proliferation and migration. Female BALB/c nude mice injected with 231 cells were used to evaluate efficacy of SH in vivo.（3） Corresponding potential target genes were extracted from Swiss Target Prediction and SEA databases. For breast cancer, disease target genes were searched with "breast cancer" as keywords from databases Therapeutic Target Disease, Drug bank, Disease Gene NET and Genetic Association Database. Furthermore, with intersection genes of SH and breast cancer were mapped, the ProteinProtein Internetwork（PPI） network of shared genes was constructed with String Database. In the last, Kyoto Encyclopaedia of Genes and Genomes（KEGG） functional annotation clusters were acquired from String database and presented as top 30 pathways.（4） Apoptosis was one of pathway indicated by enriched results and validated by flow cytometric analysis and Western blotting. RESULTS （1） 75 ingredients were identified by HPLC, including 43 flavonoids, 9 terpenoids, 9 phenolic acids, 6 alkaloids,3 coumarins or lignans, 2 anthraquinones and 3 other compounds.（2） SH lyophilized powder inhibited the proliferation and migration of both human-derived and mousederived breast cancer cells. The IC;at 24 h for 4T1,MCF7, 231-Hpa and 231-Mock are 0.89, 2.20, 1.75 and 1.27 g·L;. The healing rates of 1 g·L;SH on 4T1 are 65.80% at 9 h, 81

关键词： herb pair sculellaria barbata hedyotisdiffus breast cancer network pharmacology

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Rules of anti-infection therapy for sepsis and septic shock

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Chinese Medical Journal 2019年第5期132卷 589-596页

作者： Xiang Zhou Long-Xiang Su jia-hui zhang Da-Wei Liu Yun Long Department of Critical Care Medicine Peking Union Medical College Hospital Peking Union Medical College and Chinese Academy of Medical Sciences Beijing 100730 China

Objective: Sepsis is a deadly infection that causes injury to tissues and organs. Infection and anti-infective treatment are the eternal themes of sepsis. The successful control of infection is a key factor of resuscitation for sepsis and septic shock. This review examines evidence for the treatment of sepsis. This evidence is combined with clinical experiments to reveal the rules and a standard flowchart of anti-infection therapy for sepsis. Data Sources: We retrieved information from the PubMed database up to October 2018 using various search terms and their combinations, including sepsis, septic shock, infection, antibiotics, and anti-infection. Study Selection: We included data from peer-reviewed journals printed in English on the relationships between infections and antibiotics. Results: By combining the literature review and clinical experience, we propose a 6Rs rule for sepsis and septic shock management: right patients, right time, right target, right antibiotics, right dose, and right source control. This rule encompasses rational decisions regarding the timing of treatment, the identification of the correct pathogen, the selection of appropriate antibiotics, the formulation of a scientifically based antibiotic dosage regimen, and the adequate control of infectious foci. Conclusions: This review highlights how to recognize and treat sepsis and septic shock and provides rules and a standard flowchart for anti-infection therapy for sepsis and septic shock for use in the clinical setting.

关键词： Sepsis Infection Therapy

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Role of Triggering Receptor Expressed on Myeloid Cell-1 Expression in Mammalian Target of Rapamycin Modulation of CD8＋ T-cell Differentiation during the Immune Response to Invasive Pulmonary Aspergillosis

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Chinese Medical Journal 2017年第10期130卷 1211-1217页

作者： Na Cui Hao Wang Long-Xiang Su jia-hui zhang Yun Long Da-Wei Liu Department of Critical Care Medicine Peking Union Medical College Hospital Peking Union Medical College and Chinese Academy of Medical Sciences Beijing 100730 China

Background： Triggering receptor expressed on myeloid cell- 1 （TREM- 1） may play a vital role in mammalian target ofrapamycin （mTOR） modulation ofCD8＋ T-cell differentiation through the transcription factors T-box expressed in T-cells and eomesodermin during the immune response to invasive pulmonary aspergillosis （IPA）. This study aimed to investigate whether the roTOR signaling pathway modulates the proliferation and differentiation of CD8＋ T-cells during the immune response to I PA and the role TREM-1 plays in this process. Methods： Cyclophosphamide （CTX） was injected intraperitoneally, and Asl？e;gillus.[mnigams spore suspension was inoculated intranasally to establish the immunosuppressed IPA mouse model. After inoculation, rapamycin （2 mg-kg ·d -1） or interleukin （IL）-12 （5 μg/kg every other day） was given for 7 days. The number of CD8＋ effector memory T-cells （Tern）, expression of interferon （IFN）-y, roTOR, and ribosomal protein $6 kinase （S6K）, and the levels of IL-6, IL- 10, galactomannan （GM）, and soluble TREM- 1 （sTREM-I） were measured. Results： Viable A. fumigatus was cultured from the lung tissue of the inoculated mice. Histological examination indicated greater inflammation, hemorrhage, and lung tissue injury in both IPA and CTX ＋ IPA mice groups. The expression of mTOR and S6K was significantly increased in the CTX ＋ IPA ＋ I L- 12 group compared with the control, I PA （P = 0.01 ; P - 0.001 ）, and CTX ＋ 1PA （P = 0.034; P = 0.032） groups, but significantly decreased in the CTX ＋ IPA ＋ RAPA group （P 〈 0.001 ）. Compared with the CTX ＋ IPA group, the proportion of Tern, expression of IFN-y, and the level ofsTREM-I were significantly higher after IL-12 treatment （P = 0.024, P = 0.032, and P = 0.017, respectively）, and the opposite results were observed when the roTOR pathway was blocked by rapamycin （P 〈 0.001）. Compared with the CTX ＋ I PA and CTX ＋ I PA ＋ RAPA groups, IL-12 treatment increased IL-6 and downregulated IL- 10 as well as G M, which strengthened

关键词： CD8+ T Effector Memory Cells lmmunosuppression Invasive Pulmonary Aspergillosis Mammalian Target ofRapamycin Triggering Receptor Expressed on Myeloid Cell-1

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Relationship between red blood cell distribution width and intermediate-term mortality in elderly patients after percutaneous coronary intervention

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Journal of Geriatric Cardiology 2015年第1期12卷 17-22页

作者： Xin-Min LIU Chang-Sheng MA Xiao-hui LIU Xin DU Jun-Ping KANG Yin zhang jia-hui WU Department of Cardiology Beijing An Zhen Hospital Capital Medical University Beijing 100029 China

Background Large-scale clinical research on the relationship between red blood cell distribution width （RDW） and intermediate-term prognosis in elderly patients with coronary artery disease （CAD） is lacking. Thus, this study investigated the effects of RDW on the intermediate-term mortality of elderly patients who underwent elective percutaneous coronary intervention （PCI）. Methods Data from 1891 patients 〉 65 years old underwent elective PCI from July 2009 to September 2011 were collected. Based on preoperative median RDW （12.3%）, the patients were divided into two groups. The low RDW group （RDW 〈 12.3%） had 899 cases; the high RDW group （RDW 〉 12.3%） had 992 cases. The all-cause mortality rates of the two groups were compared. Results Patients in the high RDW group were more likely to be female and accompanied with diabetes, had lower hemoglobin level. The mean follow-up period was 527 days. During follow-up, 61 patients died （3.2%）. The postoperative mortality of the high RDW group was significantly higher than that of the low RDW group （4.3% vs. 2.0%, P = 0.004）. After adjusting other factors, multivariate Cox regression analysis revealed that preoperative high RDW was significantly associated with postoperative all-cause mortality （hazard ratio： 2.301, 95% confidence interval： 1.106-4.785, P = 0.026）. Conclusions Increased RDW was an independent predictor of the increased intermediate-term all-cause mortality in elderly CAD patients after elective PCI.

关键词： Coronary artery disease Elderly patients Percutaneous coronary intervention Red blood cell distribution width

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处分原则下的当事人诉讼行为

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现代法学 2005年第3期27卷 62-73页

作者：张家慧海南省高级人民法院海南海口570206

处分原则和辩论原则为民事诉讼的两大基石,而民事诉讼则是法院审判行为与当事人诉讼行为合力的结果,因此在处分原则的框架内对实体层面上的当事人诉讼行为进行研究,对于保障当事人处分权和规制其诉讼行为,并在此基础上更合理和科学地构... 详细信息

处分原则和辩论原则为民事诉讼的两大基石,而民事诉讼则是法院审判行为与当事人诉讼行为合力的结果,因此在处分原则的框架内对实体层面上的当事人诉讼行为进行研究,对于保障当事人处分权和规制其诉讼行为,并在此基础上更合理和科学地构筑整个民事诉讼制度无疑将具有十分重要的理论意义和现实意义。

关键词：诉讼行为处分原则当事人处分权民事诉讼制度辩论原则审判行为理论意义法院规制

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Study on the hot deformation behaviors of Al-Zn-Mg-Cu-Cr aluminum alloy

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材料科学与工程（中英文版） 2007年第1期1卷 48-54页

作者： LIN Gao-yong zhang Zhen-feng YUAN Xiao-li ZHOU jia zhang hui PENG Da-shu School of Materials Science and Engineering Central South University Changsha 410083 China

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民国基督教圣诗中的爱国主义思想探析

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湖南大学学报（社会科学版） 2014年第4期28卷 98-102页

作者：张慧佳武汉大学文学院湖北武汉430072

作为基督教文学的重要组成部分,圣诗自其诞生之日起就承载着知识分子对民族现实处境的深切关注。民国时期,再次进入中华民族视野的圣诗经历了一次本土化变革,大批怀揣爱国主义理想的基督徒投身于这一进程,并将自己对民族国家、社会历史... 详细信息

作为基督教文学的重要组成部分,圣诗自其诞生之日起就承载着知识分子对民族现实处境的深切关注。民国时期,再次进入中华民族视野的圣诗经历了一次本土化变革,大批怀揣爱国主义理想的基督徒投身于这一进程,并将自己对民族国家、社会历史的思考融入其中。基督教文学的入华扩充了民国基督徒的文学视野,激发了创作灵感,赋予了中国文学新的生机,并助推了民国知识分子爱国主义情怀的良性生成与发展。

关键词：民国基督教圣诗爱国主义

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