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一株非PSP产毒的亚历山大藻对黑褐新糠虾的急性和慢性毒性影响

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水产学报 2008年第1期32卷 111-116页

作者：张彬颜天周名江中国科学院海洋研究所海洋生态与环境科学重点实验室中国科学院海洋研究所海洋生态与环境科学重点实验室山东青岛2.6071 中国科学院研究生院北京100049

报道了一株非PSP产毒的相关亚历山大藻(Alexandrium affine,株AC-1)对甲壳类生物黑褐新糠虾(Neomysis awatschensis)的存活、生长及种群繁殖的研究结果。通过研究发现,该藻对黑褐新糠虾有急性致死作用,96 h LC50为5 000 ***-1。研究该... 详细信息

报道了一株非PSP产毒的相关亚历山大藻(Alexandrium affine,株AC-1)对甲壳类生物黑褐新糠虾(Neomysis awatschensis)的存活、生长及种群繁殖的研究结果。通过研究发现,该藻对黑褐新糠虾有急性致死作用,96 h LC50为5 000 ***-1。研究该藻各组分:藻液、藻细胞重悬液、藻细胞培养过滤液、内容物对糠虾存活的影响时,发现只有藻液和藻细胞重悬液显著降低了糠虾存活率,过滤液和内容物也有影响,但不显著,表明相关亚历山大藻活体藻细胞的毒性最强。通过对糠虾63 d的慢性毒性实验研究,发现在密度为50 ***-1藻液中,糠虾的繁殖就受到了不利影响。试验进行到63 d结束时,糠虾日最高产虾数、总产幼虾数、总产虾天数都明显减少,初次产虾时间推迟,繁殖中断增加。且该藻对黑褐新糠虾亲虾的存活、生长也有一定的影响,糠虾亲虾的存活率为对照的71%,而体长和体重分别为对照组的87.3%和97.8%,但差异尚不显著(P>0.05)。可见,相关亚历山大藻(AC-1)虽然不产生PSP毒素,但也能对甲壳类生物的种群数量和资源补充产生不利影响。

关键词：相关亚历山大藻黑褐新糠虾存活生长种群繁殖

Spatial and temporal distribution of polycyclic aromatic hydrocarbons in fine- grained sediments of the East china Sea

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Acta Geochimica 2006年第S1期 8-9页

作者： zhigang GUO1, Tian LIN1, Zongyan zhang1, Gan zhang2 1 College of marine Geosciences, Ocean University of china, qingdao 266003, china 2 State key laboratory of Organic Geochemistry, Guangzhou institute of Geochemistry, chinese academy of sciences, Guangzhou 510640, china

Antioxidation Mechanism of Haematococcus Pluvialis Cells Exposed to Superoxide Free Radicals

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Antioxidation Mechanism of Haematococcus Pluvialis Cells Exp...

Establishment of trophic continuum in the food web of the Yellow Sea and East china Sea ecosystem: Insight from carbon and nitrogen stable isotopes

中国海洋湖沼学会藻类学分会第七届会员大会暨第十四次学术讨论会

作者： zhang Xiaoli~(1,2) LIU Jianguo~(1,**) SUN Yanhong~(1,2) LIN Wei~1 1 Research and Development Center of marine Biotechnology,institute of oceanology,chinese academy of sciences,qingdao 2.6071,P.R.china 2 Graduate University of chinese academy of sciences,Beijing 100039,ER.china

Three types of Haematococcus pluvialis cells were exposed to high concentration of superoxide anion radical(O2).Their antioxidation capacities and responsive sequences were studied by analyzing malondialdehyde content and the activities of superoxide dismutases,peroxidase,catalase and ascorbate *** results showed that defensive enzymes and astaxanthin co-existed to protect the organism against oxidative stress but they varied significantly in each type of *** green cells,the antioxidative enzymes played a key role and had higher activities than that in red cells. Of the four defensive enzymes tested in green cells,superoxide dismutases was the first to eliminate O,followed by peroxidase to eliminate HO,thereafter ascorbate peroxidase and catalase initiated their own protective *** the contrary,astaxanthin was the main antioxidation mechanism and scavenged reactive oxygen species firstly in red cells. It was concluded that astaxanthin reacted with reactive oxygen species much faster and had a stronger capacity to protect lipid peroxidation than that of protective enzymes.

关键词： astaxanthin Haematococcus pluvialis lipid peroxidation reactive oxygen species

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Science china(Life sciences) 2005年第6期48卷 531-539页

作者： CAI Deling1, LI Hongyan2, TANG Qisheng3 & SUN Yao3 1. key laboratory of marine Sedimentology & environment geology, SOA, First institute of Oceanography, State Oceanic Administration, qingdao 266061, china 2. Ocean University of china, qingdao 266003, china 3. Yellow Sea Fisheries Research institute, chinese academy of Fishery sciences, qingdao 2.6071, china 1. key laboratory of marine Sedimentology & environment geology SOA First Institute of Oceanography State Oceanic Administration 266061 Qingdao China 2. Ocean University of china 266003 Qingdao China 3. Yellow Sea Fisheries Research institute Chinese Academy of Fishery Sciences 266071 Qingdao China

Stable carbon and nitrogen isotope ratios (δ13C and δ15N) are used to study the trophic structure of food web in the Yellow Sea and East china Sea ecosystem. The trophic con-tinuum of pelagic food web from phytoplankton to top preyer was elementarily established, and a trophic structure diagram in the Yellow Sea and East china Sea was outlined in combination with carbon isotopic data of benthic organisms, which is basically consistent with and makes some improvements on the simplified Yellow Sea food web and the trophic structure diagram drawn based on the biomass of main resource population during 1985―1986. This result indicates that the stable isotope method is a potential useful means for further studying the complete marine food web trophic continuum from viruses to top predators and food web stability.

关键词： food web, trophic continuum, carbon isotope, nitrogen isotope, Yellow Sea, East china Sea.

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Cloning and recombinant expression of chitosanase gene from Bacillus sp. S-1

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Cloning and recombinant expression of chitosanase gene from ...

我国“海洋1”号卫星(HY-1)CCD图像数据定量化条带消除研究

2010 First International Conference on Cellular,Molecular Biology, Biophysics and Bioengineering(CMBB 2010)

作者： Yuying SUN,Shujun WANG Jiangsu key laboratory of marine Biotechnology Huaihai institute of Technology Lianyungang,china Jiquan zhang key Lab of Experimental marine Biology institute of oceanology,chinese academy of sciences qingdao,china

In this work,we report the characterization of a chitosanase-producing bacterium isolated from *** strain was grouped under the genus Bacillus by virtue of its morphological,physiological properties and 16S rDNA gene sequence and named it Bacillus *** to the information of chitosanase full-length sequences deposited in NCBI,a pair of degenerated primes was designed and a partial sequence of chitosanase gene was obtained by polymerase chain reaction(PCR) using Bacillus sp.S-1 genome DNA as the template.A genome walking library was constructed followed as the protocol provided by CLONTECH *** flanking sequences of the 5’and 3’terminal was obtained by genome walking method and two-step PCR *** overlapped and confirmed,the full-length sequence of chitosanase from Bacillus sp.S-1 was achieved and it contained 1362 bp coding 453 amino acids(accession number is EU924147) .The predicted amino acid sequence was 96% similar to that of Bacillus cereus ATCC 14579(accession number is NC04722) .The fusion protein containing BSCHITO was produced in Escherichia coli and purified using Ni-NTA affinity *** purified rBSCHITO degraded the chitosan(the degree of deacetylation of 99%) to produce mixture of *** BSCHITO is thus an endo-chitosanase.

关键词： chitosanase Bacillus recombinant expression chitooligosaccharides

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海洋学报 2002年第6期24卷 20-33页

作者：孙凌唐军武张杰中国科学院海洋研究所山东青岛266071 国家卫星海洋应用中心北京100081 国家海洋局第一海洋研究所海洋环境科学与数值模拟国家海洋局重点实验室山东青岛266061

条带 (striping)对于大量的航天和航空传感器来说是一种普遍存在的现象 ,任何使用多个探测器进行数据获取的遥感器图像数据中均存在条带 .目前消除条带的算法主要是针对光机扫描仪 ,对于CCD相机工作做得比较少 .针对HY -1CCD线阵推扫式... 详细信息

条带 (striping)对于大量的航天和航空传感器来说是一种普遍存在的现象 ,任何使用多个探测器进行数据获取的遥感器图像数据中均存在条带 .目前消除条带的算法主要是针对光机扫描仪 ,对于CCD相机工作做得比较少 .针对HY -1CCD线阵推扫式多光谱相机的特点 ,以及发射前辐射定标的结果 ,对遥感器图像数据中的条带问题进行了预研 ,提出了一种定量化的消条带算法 .该方法根据卫星数据特性求出各探测器间的均衡化曲线以反映探测器之间的差异 ,并据此来消除条带 .实验结果表明该方法能够有效解决CCD相机的条带问题 ,而且可以保持数据原有的物理意义 ,并可与在轨辐射校正衔接 ,保证发射前后定标算法的一致性 .

关键词： CCD相机条带消除算法海洋卫星遥感图像航空传感器

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Sm-Nd isotopic geochronologyof the Yinachang Fe-Cu-REE deposit at Wuding, Yunnan Province and its genetic significance

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Science Bulletin 2005年第18期 124-130页

作者： YANG Yaomin1,2,3, TU Guanzhi1, HU Ruizhong1 & SHI Xuefa2 1. institute of Geochemistry, chinese academy of sciences, Guiyang 550002, china 2. The key laboratory of marine Sedimentology and environmental geology, The First institute of Oceanography, SOA, qingdao 266061, china 3. Northwest Geological Exploration Bureau, CNNC, Xi’an 710054, china

Sm-Nd isotope systematics of ores and fluorites places precise time constraints on REE mineralization of the Yinachang Fe-Cu-REE deposit in Yunnan Province which occur in the Proterozoic Kunyan group. The ore-related fluorites contain high abundant REE, and show a strong enrichment of LREE and a positive Eu anomaly, which is in marked contrast to post-ore fluorites that are HREE en-riched. The Sm-Nd isochron ages of the ores and fluorites are 1539±40 Ma and 1617±100 Ma, respectively, and calculated εNd(t) are ?4.6 and ?3.2. Accurate age determinations of the mineralization demonstrate that the REE mineralization took place during early Mesoproterozoic. Together with other geological evidence from the ore deposit, the primary REE-bearing ore-forming fluids are suggested to have been derived from the Enriched Mantle, and the Yinachang Fe-Cu-REE ore deposit is of volcanic exhalation- hydro-thermal sedimentary origin. This study also imply that the sudden large-scale REE mineralization in the Mesoprotero-zoic epoch may have been induced by alkaline-rich anoro-genic magmatism which occurred at marginal rifts of the continent in the Mesoproterozoic.

关键词： Kunyang rift Yinachang Fe-Cu-REE deposit fluorite Sm-Nd isotopic systematics Mesoproterozoic.

牙鲆MHC class ⅡB基因多态性及其与鱼体抗病力关系的分析

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水产学报 2006年第5期30卷 633-639页

作者：张玉喜陈松林中国水产科学院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室中国水产科学院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室山东青岛2.6071 中国海洋大学海洋生命学院山东青岛266003

用fmhcN1和fmhcC1引物分别从42尾感病个体和42尾抗病个体的基因组DNA中扩增MHC基因片段,扩增产物长度为268/280 bp。在268/280 bp的核苷酸序列中,有32个(11.4%)核苷酸位点是多态的。在其编码的61个氨基酸位点中,有13个位点是多态的,其中... 详细信息

用fmhcN1和fmhcC1引物分别从42尾感病个体和42尾抗病个体的基因组DNA中扩增MHC基因片段,扩增产物长度为268/280 bp。在268/280 bp的核苷酸序列中,有32个(11.4%)核苷酸位点是多态的。在其编码的61个氨基酸位点中,有13个位点是多态的,其中有6个位点发生在多肽结合位点上。对核苷酸替代的类型及位点进行分析,发现非多肽结合位点的非同义碱基替代率与同义碱基替代率的比值(dN/dS)(0.523)远远小于多肽结合位点的非同义碱基替代率与同义碱基替代率的比值(dN/dS)(23.091),表明氨基酸替换集中出现在exon2多肽结合位点上。分析84个个体的411个阳性克隆的测序结果,发现有13个不同的MHC classⅡB等位基因,并且分别编码13个不同的氨基酸序列。其中大部分等位基因如a,b,c,d,e,f,j,k,i,m是两个群体共有的,等位基因d在感病群体中出现的频率(23.80%)显著高于在抗病群体中出现的频率(7.14%)。而等位基因g和h只出现在13个抗病个体中,其频率分别为21.4%和9.52%;等位基因l只出现在感病群体中,其频率为19.05%。

关键词：主要组织相容性复合体 MHCⅡ多态性抗病力牙鲆

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海州湾黄鲫幼鱼的食性及其随叉长的变化

水产学报 2010年第6期34卷 921-927页

作者：郭斌张波戴芳群金显仕中国水产科学研究院黄海水产研究所山东省渔业资源与生态环境重点实验室上海海洋大学海洋科学学院

根据2008年8月在黄海海州湾海域进行的定点底拖网调查,应用K-W非参数秩检验、卡平方检验、方差分析和聚类分析等方法,对黄鲫幼鱼(35～79mm)的食物组成及其与叉长之间的关系进行研究。结果表明,黄鲫幼鱼摄食的饵料种类有40种,甲壳类(桡足... 详细信息

根据2008年8月在黄海海州湾海域进行的定点底拖网调查,应用K-W非参数秩检验、卡平方检验、方差分析和聚类分析等方法,对黄鲫幼鱼(35～79mm)的食物组成及其与叉长之间的关系进行研究。结果表明,黄鲫幼鱼摄食的饵料种类有40种,甲壳类(桡足类,磷虾类和糠虾类)是主要的饵料类群,占食物总个数的94.65%。优势饵料有小拟哲水蚤、中华哲水蚤、真刺唇角水蚤和太平洋磷虾。黄鲫的摄食强度和食物组成随叉长都有明显的变化。随着叉长的增大,磷虾类的比例有所增加,比重从3.57%增加到50.62%,而桡足类的比例则减少。Levin氏标准指数和叉长成反比,说明黄鲫幼鱼随着自身生长发育,其食性逐渐产生特化。黄鲫幼鱼在叉长达到45mm时,出现了明显的摄食转换现象。

关键词：黄鲫食物组成摄食习性叉长变化海州湾