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Science Bulletin 2017年第24期62卷 1639-1648页

作者： Chuanmiao Xie Ge zhang yuman zhang Xiaoguang Song Hongyan Guo Xiaoying Chen Rongxiang Fang State Key Laboratory of Plant Genomics Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China National Center for Plant Gene Research (Beijing) Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101. China

SRWD1, a member of the WD40 protein subfamily, is induced by salt stress in rice and its homolog in barley can bind to GAMYB, implying that SRWD1 might be involved in plant defense against environmental stress and gibberellic acid(GA) signalings. In this study, we focused on the biological functions and regulation mechanisms of SRWD1 in rice. The results showed that SRWD1 expression was repressed by GA and induced by abscisic acid(ABA). Two WRKY-family transcription factors, Os WRKY45 and Os WRKY72, were found to regulate SRWD1 expression by directly binding to the W-box region in its promoter. Transient co-expression and yeast two-hybrid analyses showed that a DELLA protein strengthened the activation of Os WRKY45 and partly relieved the suppression of Os WRKY72 by binding to ***, both SRWD1-overexpressing transgenic plants and SRWD1-knockout mutants showed dwarf phenotypes and resistance to Xanthomonas oryzae.

关键词： Phytohormone Gibberellin(GA) Abscisic acid(ABA) Signal transduction DELLA protein WRKY family protein

Discovery and demonstration of small circular DNA molecules derived from Chinese tomato yellow leaf curl virus

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Chinese Science Bulletin 2000年第15期45卷 1417-1421页

作者： YIN Qinyan zhang Ying zhang yuman LIU Yule zhang Zhongze TIAN Po Departmenl of Molecular Virology and Bioengineering Institute of MicrobiologyChinese Academy of SciencesBeijing 100080China Department of Microbiology Institute of Applied EcologyChinese Academy of SciencesShenyang 110015China

Tomato yellow leaf curl viruses belong to Begomoviruses of geminiviruses. In this work, we first found and demonstrated that the small circular DNA molecules were derived from Chinese tomato yellow leaf curl viruses (TYLCV-CHI). These small circular DNA molecules are about 1.3 kb, which are half the full-length of TYLCV-CHI DNA A. It was shown by sequence determination and analysis that there was unknown-origin sequence insertion in the middle of the small molecules. These sequences of unknown-origin were neither homologous to DNA A nor to DNA B, and were formed by recombination of virus DNA and plant DNA. Although various defective molecules contained different unknown-origin sequence insertion, all the molecules contained the intergenic region and part of the AC1 (Rep) gene. But they did not contain full ORF.

关键词： geminiviruses tomato yellow leaf curl viruses genome subgene.

RoTFL1c of Rosa multiflora has a dual-function in suppressing reproductive growth and promoting vegetative growth of Arabidopsis

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Science China(Life Sciences) 2018年第12期61卷 1599-1601页

作者： Fang Wang Lijuan Lian Yunfeng Liu yuman zhang Rongxiang Fang Qinglin Liu Department of Ornamental Horticulture and Landscape Architecture China Agriculture UniversityBeijing 100193China State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China

Dear Editor, TFL1homologues play a key role in the flowering habit of *** analyze the function of the newly isolated TFL1 gene of Rosa multiflora(Chen et al.,2013),named RoTFLlc,we performed overexpression analyses in Arabidopsis *** compared with empty vector control and wild type Arabidopsis plants,RoTFLlc overexpressing transgenic plants exhibited strong phenotypes such as a clustered habit,an increased number of rosette leaves, late flowering or failure to flower.

关键词： RoTFL1c Rosa multiflora

RcSPL1-RcTAF15b regulates the flowering time of rose (Rosa chinensis)

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Horticulture Research 2023年第6期10卷 182-194页

作者： Rui Yu Zhiying Xiong Xinhui Zhu Panpan Feng Ziyi Hu Rongxiang Fang yuman zhang Qinglin Liu Department of Ornamental Horticulture College of HorticultureChina Agricultural UniversityBeijing 100193China National Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of Sciencesand National Plant Gene Research CenterBeijing 100101China University of Chinese Academy of Sciences Beijing 101408China

Rose(Rosa chinensis),which is an economically valuable floral species worldwide,has three types,namely once-flowering(OF),occasional or re-blooming(OR),and recurrent or continuous flowering(CF).However,the mechanism underlying the effect of the age pathway on the duration of the CF or OF juvenile phase is largely *** this study,we observed that the RcSPL1 transcript levels were substantially upregulated during the floral development period in CF and OF ***,accumulation of RcSPL1 protein was controlled by *** ectopic expression of RcSPL1 in Arabidopsis thaliana accelerated the vegetative phase transition and ***,the transient overexpression of RcSPL1 in rose plants accelerated flowering,whereas silencing of RcSPL1 had the opposite ***,the transcription levels of floral meristem identity genes(APETALA1,FRUITFULL,and LEAFY)were significantly affected by the changes in RcSPL1 ***15b protein,which is an autonomous pathway protein,was revealed to interact with *** silencing and overexpression of RcTAF15b in rose plants led to delayed and accelerated flowering,***,the study findings imply that RcSPL1–RcTAF15b modulates the flowering time of rose plants.

关键词： TAF flowering floral

The Rice＇NUTRITION RESPONSE AND ROOT GROWTH＇（NRR） Gene Regulates Heading Date

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Molecular Plant 2013年第2期6卷 585-588页

作者： yuman zhang Ge zhang Na Xiao Lina Wang Yaping Fu Zongxiu Sun Rongxiang Fang Xiaoying Chen State Key Laboratory of Plant Genomics Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China National Plant Gene Research Center Beijing 100101 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China Department of Ornamental Horticulture and Landscape Architecture China Agricultural University Beijing 100193 China State Key Laboratory of Rice Biology National Rice Research Institute Hangzhou 310006 China

Dear Editor, As one of the most important developmental traits, flow-ering time has been extensively investigated in the long-day plant Arabidopsis. Multiple factors are involved, including photoperiod and/or temperature （Amasino, 2010）. Flowering locus T （FT） is known to be an important integrator of dif-ferent flowering pathways （Taoka et al., 2011）. Genes of the CONSTANS （CO） family also play key roles in regulating flow-ering time. The CO protein consists of a zinc finger and a CCT domain shared by a group of plant-specific transcription fac-tors that regulate photoperiodic flowering response and cir-cadian rhythms in Arabidopsis.

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Expression of human hepatitis C virus core antigen in tobacco plants by tobacco mosaic virus-based vector system

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Chinese Science Bulletin 2000年第1期45卷 44-48页

作者： HAN Aidong LIU Yule XIAO Li KANG Liangyi zhang yuman LI Dongtian TIAN Bo Institute of Microbiology Chinese Academy of SciencesBeijing 100080China Tianjin Medical University Tianjin 300070China

Tobacco mosaic virus (TMV) has the potential to highly express foreign gene. A novel TMV-based in trans expression system was constructed. A TMV mutant TSHc had its coat protein replaced with hepatitis C vir黶 (HCV) core antigen gene. Anotherr TMV mutant TSBD was repli-case-defective. Coinfection of the two mutants could cause systemic infection in tobacco plants by in trans complementation of their functions. TSHc could effectively replicate and assemble to viral particles, which were a little longer than that of wild-type TMV. HCV core antigen was expressed in whole tobacco plants. A similar expression level of HCV core antigen was detected on serial passages, which suggested that this viral expression system be stable.

关键词： TMV vector complementation systemic infection HCV core antigen.

水热条件下含橄榄石大洋玄武岩的摩擦特性实验研究

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地球物理学报 2024年第3期67卷 1069-1080页

作者：张钰曼张雷何昌荣中国地震局地质研究所地震动力学国家重点实验室北京100029 西藏自治区地震局拉萨850000

玄武岩作为大洋地壳上的主要成分,研究其摩擦特性是更好地探究逆冲板块边界上大地震等力学行为机理的基础.为了研究少量橄榄石矿物是否会影响玄武岩的摩擦特性,本研究选用了国际大洋钻探计划(IODP)349航次获取的玄武岩样品,其中含有少... 详细信息

玄武岩作为大洋地壳上的主要成分,研究其摩擦特性是更好地探究逆冲板块边界上大地震等力学行为机理的基础.为了研究少量橄榄石矿物是否会影响玄武岩的摩擦特性,本研究选用了国际大洋钻探计划(IODP)349航次获取的玄武岩样品,其中含有少量的橄榄石矿物.本次研究的实验温度范围为100~600℃,施加的有效正压力和孔隙水压分别为150 MPa和100 MPa.实验中玄武岩样品在300~400℃的温度条件下表现出不稳定的准静态震荡现象,并且在高温条件下(T>400℃)其剪切强度表现出显著的位移弱化现象,即摩擦系数随着剪切位移的进行而持续减小.在3 mm的剪切位移范围内,摩擦系数的变化范围为0.7~0.55.通过初步的微观剪切变形研究,我们推测橄榄石在摩擦实验过程中对一种弱矿物的生成可能起到了催化作用,但生成的弱矿物含量不足以显著地弱化断层泥的摩擦强度.同时微观构造分析也发现,随着温度的升高,断层泥的剪切构造由局部剪切向整体弥散性剪切变形转变,同时伴随着孔隙度的显著降低,因此我们认为剪切强度的位移弱化现象与流体参与下的颗粒间的压溶过程的逐步增强有关.

关键词：玄武岩橄榄石摩擦强度摩擦滑动稳定性位移弱化

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情商与教练员的领导行为:教练员效能的中介作用

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成都体育学院学报 2015年第6期41卷 90-95页

作者：张玉满广东药学院广东广州510006

研究选取260名教练员(男性=176,女性=84)为被试,通过问卷调查法对教练员情商、教练员领导行为和教练员效能进行测试,以检验教练员情商与教练员领导行为的关系,以及教练员效能的中介作用。研究结果表明:(1)教练员情商对教练员效能具有显... 详细信息

研究选取260名教练员(男性=176,女性=84)为被试,通过问卷调查法对教练员情商、教练员领导行为和教练员效能进行测试,以检验教练员情商与教练员领导行为的关系,以及教练员效能的中介作用。研究结果表明:(1)教练员情商对教练员效能具有显著影响,教练员情商的冲动性主要影响的是教练员的技术指导效能和人格塑造效能,焦虑情绪对教练员的比赛策略效能、动机激励和人格塑造均具有显著影响作用;(2)教练员的比赛策略效能能够有效预测教练员的专制行为、社会支持行为和积极反馈行为,教练员的动机激励效能对教练员的领导行为具有显著预测效应;(3)教练员效能对情商和教练员领导行为之间具有显著中介作用。

关键词：情商教练员领导行为教练员效能

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明清时期儒、医融合的思想演变及其特征

社会科学战线 2021年第12期318卷 18-24页

作者：张玉漫刘涛广州医科大学马克思主义学院广东广州511436

文章从儒、医关系发展,儒、医经典类比,儒、医思想融合三方面入手,分析归纳了明清时期儒、医融合的思想演变及其特征。明清时期的医、儒关系以互融互显为主流,医学家们多将医学经典类比于儒家经典进行论述,其中又存在着先儒后医、儒优... 详细信息

文章从儒、医关系发展,儒、医经典类比,儒、医思想融合三方面入手,分析归纳了明清时期儒、医融合的思想演变及其特征。明清时期的医、儒关系以互融互显为主流,医学家们多将医学经典类比于儒家经典进行论述,其中又存在着先儒后医、儒优于医等思想特点。清代出现的"儒易医难""儒书易读而医书难读"等思想,则是对先儒后医、儒优于医思想的批判与平衡。就思想融合而言,在人伦观和方法论方面,其特征体现为医学家对儒家孝道观、经权论的传承与运用。在宇宙论方面,其思想特征体现为医学家借助儒家阴阳论来评判与整合医家内部的理论分歧。在工夫论方面,其思想特征体现为医学家以儒家工夫论来诠释医学理论并将之成功运用于临床,进一步推动了儒、医融合从理论层面走向实践。

关键词：明清儒医融合演变

光伏-储能-热电联产综合能源系统分解协调优化运行研究

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电工技术学报 2020年第11期35卷 2372-2386页

作者：张雨曼刘学智严正张沛超上海交通大学电力传输与功率变换控制教育部重点实验室上海200240

为了实现综合能源系统电热网在各自信息隐私保护情况下达到全局最优,该文研究了光伏-储能-热电联产(CHP)综合能源系统的协调优化运行,探讨了在隐私保护前提下运用分解协调优化方法求解的可行性。该文首先建立电热耦合系统的分解优化运... 详细信息

为了实现综合能源系统电热网在各自信息隐私保护情况下达到全局最优,该文研究了光伏-储能-热电联产(CHP)综合能源系统的协调优化运行,探讨了在隐私保护前提下运用分解协调优化方法求解的可行性。该文首先建立电热耦合系统的分解优化运行模型。其次,利用Benders分解方法对模型进行求解,将模型分解为热力系统主问题和电力系统子问题,通过两个系统之间迭代计算,得出各系统设备的最优出力分配;然后,研究了基于Benders分解法的系统间交互模式,在尽可能保证主、子问题各自信息有效保护隔离的前提下,两个系统之间只需要交换隐私化后的参数及Benders约束,最大程度地保护了电/热系统的信息隐私。最后,利用三个典型场景下的算例对比分析验证了该文方法的合理性。

关键词：综合能源系统 Benders分解信息隐私保护协调优化运行

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