Discovery and demonstration of small circular DNA molecules derived from Chinese tomato yellow leaf curl virus

作     者：YIN Qinyan ZHANG Ying ZHANG Yuman LIU Yule ZHANG Zhongze TIAN Po 

作者机构：Departmenl of Molecular Virology and BioengineeringInstitute of MicrobiologyChinese Academy of SciencesBeijing 100080China Department of MicrobiologyInstitute of Applied EcologyChinese Academy of SciencesShenyang 110015China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2000年第45卷第15期

页      面：1417-1421页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：China-Israel Fund for Scientific and Strategic Research and Development  (ZYJ 01-03) 

主　　题：geminiviruses tomato yellow leaf curl viruses genome subgene. 

摘      要：Tomato yellow leaf curl viruses belong to Begomoviruses of geminiviruses. In this work, we first found and demonstrated that the small circular DNA molecules were derived from Chinese tomato yellow leaf curl viruses (TYLCV-CHI). These small circular DNA molecules are about 1.3 kb, which are half the full-length of TYLCV-CHI DNA A. It was shown by sequence determination and analysis that there was unknown-origin sequence insertion in the middle of the small molecules. These sequences of unknown-origin were neither homologous to DNA A nor to DNA B, and were formed by recombination of virus DNA and plant DNA. Although various defective molecules contained different unknown-origin sequence insertion, all the molecules contained the intergenic region and part of the AC1 (Rep) gene. But they did not contain full ORF.