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Single-molecule biotechnology for protein researches

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Chinese Journal of Chemical Engineering 2021年第2期34卷 212-224页

作者： Xiaoyan Zhuang Qian Wu aihui zhang Langxing Liao Baishan Fang Department of Chemical and Biochemical Engineering College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China The Key Lab for Synthetic Biotechnology of Xiamen City Xiamen UniversityXiamen 361005China The Key Laboratory for Chemical Biology of Fujian Province Xiamen UniversityXiamen 361005China

Cells employ proteins to perform metabolic functions and maintain active physiological state through charge transfer and energy *** processes are carried out in a narrow space precisely and rapidly,which,no doubt,bring great difficulty for their detection and ***,in recent years,the development and expansion of single-molecule technique in protein research make monitoring the dynamical changes of protein at single-molecule level a reality,which also provides a powerful tool for the further exploration of new phenomena and new mechanisms of life *** paper aims to summarize the working principle and essential achievements of single-molecule technique in protein research in recent five *** focus on not only dissecting the difference of nanopores,atomic force microscope,scanning tunneling microscope,and optical tweezers technique,but also discussing the great significance of these single-molecule techniques in investigating intramolecular and intermolecular interactions,electron transport,and conformational ***,the opportunities and challenges of the single-molecule technique in protein research are discussed,which provide a new door for single-molecule protein research.

关键词： Single-molecule protein Enzyme Nanopore Atomic force microscope Scanning tunneling microscope Optical tweezers Biotechnology Imaging

Aptamer-based cell-free detection system to detect target protein

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Synthetic and Systems Biotechnology 2021年第3期6卷 209-215页

作者： Junhong Chen Xiaoyan Zhuang Jiyang Zheng Ruofan Yang Fei Wu aihui zhang Baishan Fang XMU-China Team Xiamen UniversityXiamen361005PR China College of Chemistry and Chemical Engineering Xiamen UniversityXiamen361005PR China The Key Laboratory for Chemical Biology of Fujian Province Key Lab for Synthetic Biotechnology of Xiamen CityXiamen UniversityXiamen361005PR China

Biomarkers of disease,especially protein,show great potential for diagnosis and *** detecting a certain protein,a binding assay implementing antibodies is commonly ***,antibodies are not thermally stable and may cause false-positive when the sample composition is *** recent years,a functional nucleic acid named aptamer has been used in many biochemical analysis cases,which is commonly selected from random sequence libraries by using the systematic evolution of ligands by exponential enrichment(SELEX)*** to antibodies,the aptamer is more thermal stable,easier to be modified,conjugated,and ***,an Aptamer-Based Cell-free Detection(ABCD)system was proposed to detect target protein,using epithelial cell adhesion molecule(EpCAM)as an *** combined the robustness of aptamer in binding specificity with the signal amplification ability of CRISPR-Cas12a′s trans-cleavage activity in the ABCD *** also demonstrated that the ABCD system could work well to detect target protein in a relatively low limit of detection(50-100 nM),which lay a foundation for the development of portable detection *** work highlights the superiority of the ABCD system in detecting target protein with low abundance and offers new enlightenment for future design and development.

关键词： Aptamer CRISPR-Cas Protein detection Biomarker

Degradation strategies of pesticide residue:From chemicals tosynthetic biology

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Synthetic and Systems Biotechnology 2023年第2期8卷 302-313页

作者： Bi Ruomeng Ou Meihao Zhou Siru Geng Shichen Zheng Yixian Chen Junhong Mo Ruijie Li Yuan Xiao Gezhi Chen Xingyu Zhai Shiyi zhang aihui Fang Baishan XMU-China Team Innovative Practice Platform for Genetic Engineering Machine DesignXiamen UniversityXiamenFujian361005PR China College of Chemistry and Chemical Engineering Xiamen UniversityXiamenFujian361005PR China School of Public Health Xiamen UniversityXiamenFujian361005PR China College of Energy Xiamen UniversityXiamenFujian361005PR China School of Life Sciences Xiamen UniversityXiamenFujian361005PR China School of Pharmaceutical Science Xiamen UniversityXiamenFujian361005PR China Shenzhen Research Institute of Xiamen University Shenzhen518000PR China

The past 50 years have witnessed a massive expansion in the demand and application of ***,pesticides are difficult to be completely degraded without intervention hence the pesticide residue could pose a persistent threat to non-target organisms in many *** aim at the problem of the abuse of pesticide products and excessive pesticide residues in the environment,chemical and biological degradation methods are widely developed but are scaled and insufficient to solve such a *** recent years,bio-degradative tools instructed by synthetic biological principles have been further studied and have paved a way for pesticide *** the customized design strategy and standardized assembly mode,the engineering bacteria for multi-dimensional degradation has become an effective tool for pesticide residue *** review introduces the mechanisms and hazards of different pesticides,summarizes the methods applied in the degradation of pesticide residues,and discusses the advantages,applications,and prospects of synthetic biology in degrading pesticide residues.

关键词： Synthetic biology Degradation Remediation Biological engineering Pesticide residue

消能支撑加固底部框架砌体房屋的弹塑性地震反应分析

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建筑结构学报 2005年第4期26卷 58-63页

作者：秦从律张爱晖浙江大学建筑设计研究院浙江杭州310027 浙江大学建筑工程学院浙江杭州310027

本文采用弹塑性时程分析方法,对底部框架多层砌体房屋在不同烈度罕遇地震作用下进行了抗震性能评估;研究了在底部框架内增设消能外包混凝土无粘结钢支撑对房屋抗震性能的影响;对增设消能支撑加固方案和增设抗震墙加固方案进行了对比分... 详细信息

本文采用弹塑性时程分析方法,对底部框架多层砌体房屋在不同烈度罕遇地震作用下进行了抗震性能评估;研究了在底部框架内增设消能外包混凝土无粘结钢支撑对房屋抗震性能的影响;对增设消能支撑加固方案和增设抗震墙加固方案进行了对比分析。分析结果表明,在底层框架砌体房屋的底层增设消能支撑可显著降低底层框架的最大层间位移,控制结构的塑性损伤,提高结构的抗震性能。增设消能支撑加固方案与增设抗震墙加固方案相比,结构加固更合理、更易实施。

关键词：底部框架砌体房屋消能支撑加固抗震性能截面纤维模型弹塑性时程分析

Effects of Exogenous NO on Active Oxygen Metabolism in Cucumber Seedlings under NaCl Stress

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Agricultural Biotechnology 2014年第3期3卷 4-6,10页

作者： aihui zhang Shinong ZHU Qiulan YANG Jingjing LU College of Horticulture Jinling Institute of Technology

Employing nutrient solution hydroponic method,the effects of exogenous nitric oxide（NO）on the growth and active oxygen metabolism in cucumber（Cucumis sativus L.）seedlings under NaCl stress were *** results indicated that NaCl treatment significantly inhibited the growth of cucumber seedlings,while exogenous NO could significantly alleviate the inhibitory effects of NaCl stress on seedling ***,0.1 mmol/L SNP treatment exhibited better effects than 0.5 mmol/L SNP treatment on alleviating NaCl *** 0.1 mmol/L NaCl stress,adding 0.1 mmol/L exogenous NO could significantly decrease the generation rate of O·-2and MDA content,significantly improve soluble protein content and enhance the activities of SOD,POD and CAT,thus reducing the damage of salt stress to cucumber seedlings.

关键词： Cucumis sativus L. NO Active oxygens Salt stress

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氧化还原酶催化机理研究的沿革和新探

科学通报 2021年第10期66卷 1233-1239页

作者：庄小燕张瑷珲方柏山厦门大学化学化工学院化学工程与生物工程系厦门361005 厦门大学厦门市合成生物学重点实验室厦门361005 厦门大学福建省化学生物学重点实验室厦门361005

氧化还原酶在生命代谢中起着至关重要的作用.自1951年经典氧化还原酶催化机制的提出,对该机制的研究和修饰一直是热点.本文首先系统地介绍氧化还原酶催化机理研究的沿革,提出两个值得探讨的科学问题:(1)细胞内与氧化还原酶结合的辅酶经... 详细信息

氧化还原酶在生命代谢中起着至关重要的作用.自1951年经典氧化还原酶催化机制的提出,对该机制的研究和修饰一直是热点.本文首先系统地介绍氧化还原酶催化机理研究的沿革,提出两个值得探讨的科学问题:(1)细胞内与氧化还原酶结合的辅酶经历氧化或还原后是否非得解离?(2)细胞内NAD(P)H依赖型氧化还原酶与其相应的辅酶是否存在自组装?基于本课题组前期关于NAD(P)H依赖型氧化还原酶催化实验中的新发现,近期以NADH依赖型甲酸脱氢酶为研究对象,应用扫描隧道显微镜断裂连接系统考察单分子酶结合辅酶、催化底物过程的实时导电性,介绍我们对氧化还原酶催化机理的新探索,即氧化还原酶催化反应后辅酶是否会从反应空腔脱离.基于该机理的讨论和新探索,既是基于传统机理的进一步研究,同时有望更新传统的全细胞发酵、多酶耦联催化、酶活性检测、生物传感器等技术.

关键词：氧化还原酶催化机理单分子酶辅酶

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陕西省农地经营权流转服务体系建设浅析

中国农业资源与区划 2016年第3期37卷 156-161页

作者：张爱辉西安外事学院

2011年《陕西省人民政府关于促进农村土地承包经营权流转的指导意见》发布以来,陕西省农地经营权流转发展迅速,农业生产效益得到明显提高;但仍存在土地产权不明晰、农地经营权流转市场不成熟、农地经营权流转法律法规不健全等问题,严重... 详细信息

2011年《陕西省人民政府关于促进农村土地承包经营权流转的指导意见》发布以来,陕西省农地经营权流转发展迅速,农业生产效益得到明显提高;但仍存在土地产权不明晰、农地经营权流转市场不成熟、农地经营权流转法律法规不健全等问题,严重限制农地经营权流转的速度与范围。文章采取实地查勘、问卷调查、走访农户、搜集数据及逻辑分析等方法,针对陕西省农地经营权流转的现状、问题、成因进行实地调研,并提出加强陕西省农地经营权流转服务体系建设的合理化建议:(1)通过土地确权、规范农地经营权流转流程,提高农地经营权流转效率;(2)通过发展现代农业电商平台、保险、智库、创客空间,提高农业收益。

关键词：农地经营权流转服务体系农业电商平台农业创客农业智库

Corrigendum to“Aptamer-based cell-free detection system to detect target protein”[Synth Syst Biotechnol 6(3)(2021)209–215]

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Synthetic and Systems Biotechnology 2023年第2期8卷 339-339页

作者： Junhong Chen Xiaoyan Zhuang Jiyang Zheng Ruofan Yang Fei Wu aihui zhang Baishan Fang XMU-China Team College of Chemistry and Chemical EngineeringXiamen UniversityXiamen361005PR China The Key Laboratory for Chemical Biology of Fujian Province Key Lab for Synthetic Biotechnology of Xiamen CityXiamen UniversityXiamen361005PR China

The authors regret that following the publication of the original article,the authors noticed that the“Affiliation a”were *** should be“XMU-China Team,College of Chemistry and Chemical Engineering,Xiamen University... 详细信息

关键词： Xiamen noticed (3)

钢筋混凝土梁加固时的既有荷载对其抗弯性能的影响

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土木工程学报 2006年第1期39卷 13-20页

作者：李贵炳张爱晖金伟良浙江大学浙江杭州310027

模拟实际构件的加固及受力过程,对梁在承受不同荷载作用时外贴CFRP加固进行了抗弯试验和理论分析。试件包括16根外贴CFRP加固梁和2根对比梁。试验参数为:纵筋配筋率、CFRP加固量、粘贴纤维布时持载大小。论文对持载的大小对梁的抗弯性... 详细信息

模拟实际构件的加固及受力过程,对梁在承受不同荷载作用时外贴CFRP加固进行了抗弯试验和理论分析。试件包括16根外贴CFRP加固梁和2根对比梁。试验参数为:纵筋配筋率、CFRP加固量、粘贴纤维布时持载大小。论文对持载的大小对梁的抗弯性能的影响进行了详细的分析。试验和分析结果表明:采用CFRP加固的钢筋混凝土梁的刚度较普通钢筋混凝土梁的刚度都有明显提高,纤维布对以抗弯为主的构件具有增强刚度、控制挠度的作用。纤维加固时梁所持荷载的大小,对梁的刚度有较大影响,特别是对屈服阶段的刚度影响较大;但对梁的屈服荷载及极限承载力影响较小,可以忽略其影响。论文的试验现象及分析结果都有新颖之处,对相应规范的编制及工程应用有较高的参考价值。

关键词：承载力刚度持载 CFRP 配筋率