Celastrol promotes apoptotic cell death in children neuroblastoma cells through caspases dependent pathway

作     者：YANG Ye ZHANG Aihui LI Anmao YANG Ye;ZHANG Aihui;LI Anmao

作者机构：Department of Thoracic SurgeryXi'an Children's HospitalXi'an 710003China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2022年第42卷第6期

页      面：877-884页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the Scientific Research Project of Shaanxi Provincial Department of Health:the Protective Role of Celastrol and its Active Mechanisms in Neuroblastoma Treatment (2020A1515003129) 

主　　题：neuroblastoma child celastrol apoptosis poly(ADP-ribose)polymerases 

摘      要：OBJECTIVE: To investigate the in-depth pharmacological mechanisms of celastrol in children neuroblastoma treatment. METHODS: In the current study, we examined the effects of celastrol on children neuroblastoma cells viability and proliferation by cell counting kit-8 assay and colony formation assay. Annexin V-FTIC and PI staining were applied to determine cell apoptosis after celastrol treatment. ROS generation levels were examined by 2′, 7′-dichloroflfluorescin diacetate. RESULTS: We found that celastrol could suppress the proliferation of children neuroblastoma cells with few effects on normal cell lines in vitro. Further mechanisms studies have shown that celastrol inhibited cell cycle progression and induced cell apoptosis in QDDQ-NM and SH-SY5Y cells. In addition, ROS production might involve in celastrol-mediated apoptotic cell death in children neuroblastoma cells by activating caspase death pathway. CONCLUSIONS: Our findings demonstrated that celastrol could promote ROS generation-induced apoptosis in neuroblastoma cell by activating caspase death pathway. These findings suggested that celastrol might be a potential novel anti-neuroblastoma agent with minor cytotoxicity.