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Cyclin-Dependent Kinase 9 Inhibition Prolongs Allograft Survival through inhibiting alloreactive CD4+ T cells and preserving the activities of Treg Cells

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Cyclin-Dependent Kinase 9 Inhibition Prolongs Allograft Surv...

第十届全国免疫学学术大会

作者： Yang zhan guihua Hou 山东大学医学院实验核医学研究所

Background and Purpose:Recently,it was reported that cyclin-dependent kinase 9(CDK9)may be as the critical target for the induction of anti-inflammatory ***,a role for CDK9 involved in allotransplantation tolerance has not been *** objective of this study is to determine whether CDK9 inhibition effectively suppresses allorejection and induce donor-specific ***:We constructed allogeneic mice skin transplant model using C57BL/6 mice as donors and SCID mice as *** 21 days of allotransplantation,8×106 purified CD4+T cells from spleens of wild type BABL/c mice,with/without CDK9 inhibitor(PHA767491)treatment,were adoptively *** recipients’splenocytes,skin grafts and draining lymph nodes were harvested on day 12(acute rejection occurred)after *** change of CDK9associated genes(Cyclin T1 and Pol II)and Th1/Th2 type cytokines(IFN-γ,IL4,IL10)were determined by western blotting and antibody *** recipient’s splenocytes were stained against CD4,CD25 and Fox P3,then analyzed by flow *** vitro,the effect of PHA767491 on the proliferation of CD4+CD25-Teff was investigated by mixed lymphocyte ***:The allograft survival in PHA767491-group has remarkably ***9 and Pol II,both phosphorylatory and total protein in recipients’splenic CD4+T cells and skin grafts were declined in PHA767491-treated group,(p<0.05).However,Cyclin T1 did not show a significant difference *** array results showed that PHA767491treatment suppressed Th1 type cytokine expression in graft,along with IFN-γlevel decreasing by 8.2-fold,while Th2type cytokine,IL-4 and IL-10 level respectively increasing by 4.8-fold and by ***,PHA767491-treated Teff showed a lower preliferative response(%inhibition,71.4%).We also observed markedly increased frequencies of Tregs in PHA767491-treated *** results indicated that CDK9 inhibition could mediate an effective tolerance to al

关键词： CDK9 Regulatory T cell Alloreactive T cell Allograft

Primary Haemangiopericytoma outside Muscle Cone in Fossa Orbitalis:A Case Report and Review

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Eye Science 2012年第4期27卷 205-209页

作者： Xiaofen Ma Junzhang Tian guihua Jiang Jin Fang Wenfeng zhan Department of Radiology Guangdong NO.2 Provincial People's Hospital

Purpose:Orbital haemangiopericytoma (HPC) is a rare tumor with great histological variability and unpredictable clinical and biological *** precise cell type origin is uncertain. Method:A case report is provided. Results:A 24-year-old male patient visited an ophthalmologist because of proptosis, strabismus, and movement limitation of the right eye one year ago. These symptoms worsened rapidly. Histopathology,MRI methods and microscopy coil were applied to study the features of *** tumor was removed and the histological examination revealed that it was a primary HPC of the orbit. Conclusion:MRI determined the size,location, circumscription, morphological detail, growth pattern, and relationship between masses and surrounding *** genesis location of this HPC was rare,and its internal morphological characters were different from cases reported in the *** new type of microscope showed more details of tumor MR image characters objectively.

关键词：病例报告肿瘤组织审查发生位置形态特征生物学行为病理组织学组织学检查

NfiS,a species-specific regulatory noncoding RNA of Pseudomonas stutzeri,enhances oxidative stress tolerance in Escherichia coli

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NfiS,a species-specific regulatory noncoding RNA of Pseudomo...

中国生物工程学会第十三届学术年会暨2019年全国生物技术大会

作者： guihua Hu Tao Hu Yuhua zhan Wei Lu Min Lin Yunhong Huang Yongliang Yan College of Life Sciences Jiangxi Normal University Biotechnology Research Institute Chinese Academy of Agricultural Sciences

Noncoding RNAs(ncRNAs) can finely control the expression of target genes at the posttranscriptional level in prokaryotes. Regulatory small RNAs(sRNAs) designed to control target gene expression for applications in metabolic engineering and synthetic biology have been successfully developed and used. However, the effect on the heterologous expression of speciesor strain-specific ncRNAs in other bacterial strains remains poorly understood. In this work, a Pseudomonas stutzeri species-specific regulatory ncRNA, NfiS, which has been shown to play an important role in the response to oxidative stress as well as osmotic stress in P. stutzeri A1501, was cloned and transferred to the Escherichia coli strain Trans10. Recombinant NfiS-expressing E. coli, namely, Trans10-nfiS,exhibited significant enhancement of tolerance to oxidative stress. To map the possible gene regulatory networks mediated by NfiS in E. coli under oxidative stress, a microarray assay was performed to delineate the transcriptomic differences between Trans10-nfiS andwild-type E. coli under H2 O2 shock treatment conditions. In all, 1184 genes were found to be significantly altered, and these genes were divided into mainly five functional categories: stress response, regulation, metabolism related, transport or membrane protein and unknown function. Our results suggest that the P. stutzeri species-specific ncRNA NfiS acts as a regulator that integrates adaptation to H2 O2 with other cellular stress responses and helps protect E. coli cells against oxidative damage.

关键词： Pseudomonas stutzeri A1501 NfiS Recombinant Escherichia coli Oxidative stress Regulatory element

Environmental effect of anr gene on nitrogen fixation and biofilm formation in Pseudomonas stutzeri A1501 at low levels of oxygen

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Environmental effect of anr gene on nitrogen fixation and bi...

中国生物工程学会第十三届学术年会暨2019年全国生物技术大会

作者： Rasheed Muhammad Ali Hu guihua zhang Hongyang Liu Yichao Yaqun Liu Lu Wei zhan Yuhua Yan Yongliang Lin Min Biotechnology Research Institute Chinese Academy of Agricultural Science

Nitrogenase activity is a very distinct property of Pseudomonas stutzeri A1501. It is carried out in the presence of microaerophilic condition that is in oxygen limited condition. The biofilm formation is at its best in minimal media at nitrogen depletion condition Anr, a global transcription regulator that promotes biofilm formation. Anr absence plays a positive role in nitrogen fixation by regulating nifA, nifH and ntrC. The nitrogenase activity in anr insersion mutant was significantly down regulated. At different concentration of oxygen the nitroagenase activity was substantially differentiated. The qRT-PCR shows down regulation of nif island gene at nitrogen fixation condition. The finding suggests that different oxygen concentration in the environment play differential role against the nitrogenase activity and biofilm formation. The qRT-PCR results for rpoS during biofilm formation was up-regulated showing that low level of oxygen it has a signaling effect causing Anr to expresses which in turns controls not only rsm XYZ but also regulate the biofilm formation. Effect of Oxygen stress shows no significant effect on the survival of pseudomonas stutzeri A1501 which suggests that oxidative stress has no role on anr regulation.

关键词： Pseudomonas stutzeri A1501 biofilm formation nitrogen fixation and oxygen limitation

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中藏药独一味防治类风湿关节炎概述

中国现代应用药学 2023年第6期40卷 856-864页

作者：陈瑞鑫詹琥珀康点点蒋桂华蒋运斌成都中医药大学药学院成都611137 成都中医药大学西南特色中药资源国家重点实验室成都611137 成都中医药大学民族医药学院成都611137 西南大学药学院·中医药学院重庆400715

独一味为青藏高原珍贵药用植物,是中国藏、蒙、纳西等民族民间常用草药之一,中国药典2020年版载其具有活血止血、祛风止痛的功效。类风湿关节炎是风湿免疫科的常见疾病,全球发病率已达1%,且目前在国内外均缺乏特效治疗方法。研究表明,... 详细信息

独一味为青藏高原珍贵药用植物,是中国藏、蒙、纳西等民族民间常用草药之一,中国药典2020年版载其具有活血止血、祛风止痛的功效。类风湿关节炎是风湿免疫科的常见疾病,全球发病率已达1%,且目前在国内外均缺乏特效治疗方法。研究表明,中医药和藏药在延缓类风湿关节炎骨破坏等方面具有疗效好、不良反应少、长期适用等优点,中藏药独一味作为祛风止痛的药物具有良好的防治类风湿关节炎的潜力和发展前景。因此,本研究对独一味防治类风湿关节炎的相关本草记载和研究现状进行总结,旨在为临床使用独一味治疗类风湿关节炎提供一定的理论依据。

关键词：独一味中藏药类风湿关节炎研究现状

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八段锦治疗失眠症的研究进展

中国医药科学 2016年第5期6卷 37-39,43页

作者：熊桂华张展长春中医药大学附属医院吉林长春130021 吉林大学中日联谊医院吉林长春130033

八段锦作为中医药养生保健体系中传统的健身运动,其动作简单、形式多样、功效显著,既能调节改善人的不良心理状态,增强人体脏腑功能、提高身体素质和机能,增强防病抗病及抗衰老能力,并能辅助治疗一些慢性疾病,如亚健康、糖尿病、脂质代... 详细信息

八段锦作为中医药养生保健体系中传统的健身运动,其动作简单、形式多样、功效显著,既能调节改善人的不良心理状态,增强人体脏腑功能、提高身体素质和机能,增强防病抗病及抗衰老能力,并能辅助治疗一些慢性疾病,如亚健康、糖尿病、脂质代谢紊乱、原发性高血压、腰肌劳损、颈椎病、肩周炎、骨关节炎、慢性乙型病毒性肝炎等,适应于不同人群锻炼,对居民身心健康有很好的促进作用,有着深厚的群众基础,是值得推广的保健运动。

关键词：八段锦健身气功失眠研究近展

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中医治疗失眠症的研究进展

中国医药科学 2016年第3期6卷 46-48,163页

作者：熊桂华张展长春中医药大学附属医院吉林长春130021 吉林大学中日联谊医院吉林长春130033

失眠又称入睡和维持睡眠障碍(DIMS),是指无法入睡或无法保持睡眠状态,导致睡眠不足,以经常不能获得正常睡眠为特征的一种常见病证,祖国医学称之为"不寐""不得眠""不得卧""目不瞑"等,主要病因病机为脏腑功能紊乱,阴阳失调。随着社会生活... 详细信息

失眠又称入睡和维持睡眠障碍(DIMS),是指无法入睡或无法保持睡眠状态,导致睡眠不足,以经常不能获得正常睡眠为特征的一种常见病证,祖国医学称之为"不寐""不得眠""不得卧""目不瞑"等,主要病因病机为脏腑功能紊乱,阴阳失调。随着社会生活节奏的加快、压力增大,失眠症的发生率呈不断上升趋势。很多患者使用镇静剂治疗虽有一定疗效,但长期使用副作用较大,且易产生依赖和成瘾性。近年来,中医特色疗法以其独特的安全、有效、无毒副作用等优点被广泛应用于临床失眠治疗,现将有关中医疗法在失眠方面的应用综述如下。

关键词：失眠症中医药作用机制综述

三叉神经痛患者脑灰质结构的变化:一项基于体素的形态学测量

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南方医科大学学报 2015年第8期35卷 1180-1183页

作者：颜剑豪李盟汪天悦詹文峰江桂华广东省第二人民医院影像科广东广州510317

目的运用基于体素的形态学测量(VBM)方法分析三叉神经痛患者(TNP)和正常对照组间全脑灰质体积的差异。方法采用PHILIPS 1.5T磁共振扫描仪对28例TNP患者和28例正常对照进行全脑高分辨结构像扫描。采用SPM8对采集的三维结构像数据进行预处... 详细信息

目的运用基于体素的形态学测量(VBM)方法分析三叉神经痛患者(TNP)和正常对照组间全脑灰质体积的差异。方法采用PHILIPS 1.5T磁共振扫描仪对28例TNP患者和28例正常对照进行全脑高分辨结构像扫描。采用SPM8对采集的三维结构像数据进行预处理,利用DARTEL对全脑结构进行VBM分析,并以双样本t检验比较两组被试灰质体积的差异。结果与正常对照组相比,TNP显示双侧大脑半球广泛脑区的灰质体积减少,这些减少的区域主要位于双侧颞中回,双侧额上回,额中回,左侧中央前后回,右侧梭状回,前扣带回等区域。结论 VBM揭示了TNP存在广泛脑区灰质密度异常,这些脑区基本属于疼痛的感知和处理区域。此外,这种脑结构改变有助于对TNP神经机制的探讨。

关键词：三叉神经痛灰质体积 VBM 磁共振成像

Molecular genetic dissection of QTL regulating rapeseed yield

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Molecular genetic dissection of QTL regulating rapeseed yiel...

2017年中国作物学会学术年会

作者： Jiaqin Shi Jing Liu Jiepeng zhan Na Li Yuhua Yang Dongji Song JiangYe Wei Peng JiangJiang zhang Xinfa Wang Wei Hua guihua Liu Hanzhong Wang Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture

Yield is the most important and complex trait for the genetic improvement of *** yield in rapeseed is multiply determined by its three components:pod number,seed number per pod and seed *** analysis using several representative lines with extreme trait value showed that the natural variation of seed weight is controlled mainly by maternal genotype,whereas seed number per

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