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数值孔径对飞秒激光角膜切削的影响

中国激光 2024年第9期51卷 247-253页

作者：吕海军王雨李华明张卓宇赵新恒吕晓华刘秀丽曾绍群华中科技大学武汉光电国家研究中心高端生物医学成像重大科技基础设施生物医学光子学教育部重点实验室BrittonChance生物医学光子学研究中心湖北武汉430074 天津市眼科医院南开大学附属眼科医院天津医科大学眼科临床学院南开大学眼科学研究院天津300020

飞秒激光得益于其精准、微创等优势被广泛应用于屈光疾病的治疗。在飞秒激光屈光手术中,光学系统的数值孔径是影响手术效果的重要参数。本研究旨在探讨数值孔径对飞秒激光角膜基质切削质量的影响规律,以帮助临床医生更好地选择合适的手... 详细信息

飞秒激光得益于其精准、微创等优势被广泛应用于屈光疾病的治疗。在飞秒激光屈光手术中,光学系统的数值孔径是影响手术效果的重要参数。本研究旨在探讨数值孔径对飞秒激光角膜基质切削质量的影响规律,以帮助临床医生更好地选择合适的手术参数。选用0.16、0.30、0.80三种数值孔径进行离体动物角膜的飞秒激光切削实验,并通过气泡尺寸与凋亡细胞比例评估激光切削质量与基质细胞损伤程度。实验结果显示:气泡体积随着数值孔径的增大而减小,高数值孔径下切割更易实现基质层的分离;上述三种数值孔径下的基质细胞损伤比例分别为9.4%、4.9%和1.0%,基质细胞的损伤比例随着数值孔径的增大而明显下降。因此,增大数值孔径有助于提高飞秒激光角膜基质切削的安全性。

关键词：激光技术飞秒激光角膜数值孔径气泡细胞损伤

Femtosecond pulse laser scanning using Acousto-Optic Deflector

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Science China(Physics,Mechanics & Astronomy) 2009年第5期52卷 685-692页

作者： zeng shaoqun LUO QingMing LI DeRong Lü XiaoHua Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan 430074China

Acousto-optic modulation of the femtosecond laser beam has wide potential in various fields such as microscopic imaging, micromachining and optical storage because of its rapidity, high accuracy and good repeatability. This paper reviews the latest research on acousto-optic modulation of femtosecond laser, describes the effects of spatial and temporal dispersion of such lasers after acousto-optic modulation, introduces the theoretical outcomes on pulse width evolution of the Gaussian laser pulses after acousto-optic modulation, discusses the characteristics of contemporary dispersion-compensating techniques for the acousto-optic modulation of femtosecond laser, and finally refers to the challenges of this technique.

关键词： femtosecond laser acousto-optic modulation dispersion microscopic imaging laser machining optical storage

QUANTIFYING THE SHORT LIFETIME WITH TCSPC-FLIM:FIRST MOMENT VERSUS FITTING METHODS

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Journal of Innovative Optical Health Sciences 2013年第4期6卷 1-10页

作者： LINGLING XU ZHONG-CHAO WEI shaoqun zeng ZHEN-LIHU ANG Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology Wuhan 430074 P.R.China Key Laboratory of Biomedical Photonics of Ministry of Education Department of Biomedical Engineering Huazhong University of Science and Technology Wuhan 430074 P.R.China Laboratory of Nanophotonic Functional Materials and Devices South China Normal University Guangzhou 510006P.R.China

Combing the time corelated single photon counting(TCSPC)with fuorescence lifetime imaging microscopy(FLIM)provides promising opportunities in revealing important information on the microenvironment of cells and tissues,but the applications are thus far mainly limited by the accuracy and precision of the TCSPC-FLIM *** we present a comprehensive in-vestigation on the perforance of two data analysis methods,the first moment(M_(1))method and the conventional least squares(Fitting)method,in quantifying fuorescence *** found that the Mp method is more superior than the Fitting method when the lifetime is short(70-400ps)or the signal intensity is weak(<10^(3) photons).

关键词： Fluorescence lifetimne imaging tim-correlated single photon counting the distribution of the first moment(M_(1))

OBSERVING NEURONAL ACTIVITIES WITH RANDOM ACCESS TWO-PHOTON MICROSCOPE

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Journal of Innovative Optical Health Sciences 2009年第1期2卷 67-71页

作者： YUXIANG WU XIULI LIU WEI ZHOU XIAOHUA LV shaoqun zeng Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 China

As a second messenger in signal transduction,calcium ion plays a very important role in neuronal information processing and *** by the imaging technique,it is difficult to simultaneously perform deep tissue imaging and measure intracellular free calcium concentration([Ca^(2+)]i)in different compartments of neurons in brain slice *** means of random access two-photon microscopy,which provides high optical penetration into tissues and low photo damage,we successfully measured[Ca^(2+)]i of different parts of pyramidal neurons in neocortical layer V in rat brain slices with high spatial and temporal *** the patch clamp technique,we stimulated the soma with depolarizing current and explored the dynamics of calcium in pyramidal neurons.

关键词： Neuronal activity two-photon microscopy imaging

Imaging the structure and organization of mouse cerebellum and brain stem with second harmonic generation microscopy

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Chinese Optics Letters 2017年第9期15卷 7-11页

作者： Xiuli Liu Daozhu Hua Ling Fu shaoqun zeng Britton Chance Center for Biomedical Photonics School of Engineering Sciences Wuhan National Laboratory for Optoelectronics--Huazhong University of Science and Technology Wuhan 430074 China MoE Key Laboratory for Biomedical Photonics Department of Biomedical Engineering Huazhong University of Science and Technology Wuhan 430074. China

To visualize the structure and organization of the brain is a fundamental requirement in the research of neuroscience. Here, combining with two-photon excitation fluorescence microscopy and transgenetic mouse GAD67,we demonstrate a custom-built second harmonic generation（SHG） microscope to discriminate brain layers and sub regions in the cerebellum and brain stem slices with cellular resolution. In particular, the cell densities of neurons in different brain layers are extracted due to the cell soma appearing as dark shadow on an SHG ***, the axon initial segments of the Purkinje cell are easily recognized without labeling, which would be useful for guiding micropipettes for electrophysiology.

关键词： SHG Imaging the structure and organization of mouse cerebellum and brain stem with second harmonic generation microscopy

FluxExplorer: A general platform for modeling and analyses of metabolic net-works based on stoichiometry

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Chinese Science Bulletin 2006年第6期51卷 689-696页

作者： LUO Ruoyu LIAO Sha zeng shaoqun LI Yixue LUO Qingming Hubei Bioinformatics and Molecular Imaging Key Laboratory Huazhong University of Science and Technology Wuhan 430074China Shanghai Center for Bioinformation Technology Shanghai 200233China These authors contributed equally to this work

Stoichiometry-based analyses of meta- bolic networks have aroused significant interest of systems biology researchers in recent years. It is necessary to develop a more convenient modeling platform on which users can reconstruct their network models using completely graphical operations, and explore them with powerful analyzing modules to get a better understanding of the properties of metabolic systems. Herein, an in silico platform, FluxExplorer, for metabolic modeling and analyses based on stoichiometry has been developed as a publicly available tool for systems biology research. This platform integrates various analytic approaches, in- cluding flux balance analysis, minimization of meta- bolic adjustment, extreme pathways analysis, shadow prices analysis, and singular value decom- position, providing a thorough characterization of the metabolic system. Using a graphic modeling process, metabolic networks can be reconstructed and modi- fied intuitively and conveniently. The inconsistencies of a model with respect to the FBA principles can be proved automatically. In addition, this platform sup- ports systems biology markup language (SBML). FluxExplorer has been applied to rebuild a metabolic network in mammalian mitochondria, producing meaningful results. Generally, it is a powerful and very convenient tool for metabolic network modeling and analysis.

关键词：新陈代谢化学计量学系统生物学流量平衡分析计算模型

DIC imaging for identification of motor and sensory nerves

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Journal of Innovative Optical Health Sciences 2016年第5期9卷 1-7页

作者： Dayu Chen Yuxiang Wu Tao Sui shaoqun zeng Xiaojian Cao Xiaohua Lv Department of Neurosurgery Wuhan General Hospital of Guangzhou Military Command of PLA 627 Wuluo Road Wuhan 430070P.R.China Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 P.R.China School of Physical Education Jianghan University Wuhan 430056P.R.China First A±liated Hospital of Nanjing Medical University Nanjing 210029 P.R.China

Identification of motor and sensory nerves is important in applications such as nerve injury *** practice relies on time consuming staining methods for this ***,we use laser scanning infrared diferential interference contrast(IR-DIC)microscopy for label-free observation of the two types of *** and dorsal nerve roots of adult beagle dogs were collected and sections of different thicknesses were imaged with an IR-DIC *** texture patterns of the IR-DIC images of the motor and sensory nerve can be distinguished when the section thickness increases to 40 *** suggests that nerve fibers in motor and sensory nerves have different distribution *** result hints a potential new way for more rapid identification of nerve type in peripheral nerve repair surgery.

关键词： Differential interference microscopy nerve repair nerve root image pattern.

Modulation of synchronous calcium oscillations in hippocampal neurons by photostimulation of astrocytes with femtosecond laser

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Chinese Science Bulletin 2010年第30期55卷 3436-3440页

作者： ZHAO Yuan LIU XiuLi ZHANG Yuan ZHOU Wei zeng shaoqun Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Tech-nology Wuhan 430074 China

A large body of evidence indicates that astrocytes play an important role in a range of brain functions through calcium (Ca2+) signaling. Experimentally evoking Ca2+ signaling is a useful technique for investigating the functions of astrocytes. However,conventional stimulation methods typically have poor spatio-temporal precision,and some are invasive. Our group has developed a technique to overcome these problems,in which astrocytes are photostimulated with a femtosecond laser. In the current study,we applied this method to a hippocampal neural network to explore astrocytic functions in detail. The results revealed that applying photostimulation to astrocytes in a cultured hippocampal astrocyte-neuron network caused the following changes:(i) Syn-chronous Ca2+ oscillations in neurons were induced; (ii) spontaneous Ca2+ synchrony instantaneously emerged; and (iii) high-frequency spontaneous Ca2+ synchrony was regulated. Thus,astrocytic Ca2+ signaling evoked by photostimulation was found to modulate synchronous Ca2+ oscillations in hippocampal neurons. We propose that photostimulation with a femtosecond laser will serve as a powerful tool in investigating astrocytic functions at the network level.

关键词：星形胶质细胞海马神经元飞秒激光钙振荡同步性调制细胞功能神经网络

Construction and visualization of high-resolution three-dimensional anatomical structure datasets for Chinese digital human

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Chinese Science Bulletin 2008年第12期53卷 1848-1854页

作者： LI AnAn LIUQian zeng shaoqun TANG Lei ZHONG ShiZhen LUO QingMing The Key Laboratory for Biomedical Photonics of the Ministry of Education Wuhan National Laboratory for Optoelectronics Department of Biomedical Engineering Huazhong University of Science and Technology Wuhan 430074 China Department of Anatomy Southern Medical University G uangzhou 510515 China

The objective of the China Digital Human Project (CDH) is to digitize and visualize the anatomical structures of human body. In the project, a database with information of morphology, physical charac-teristics and physiological function will be constructed. The raw data of CDH which was completed in the Southern Medical University is employed. In Huazhong University of Science and Technology (HUST), the frozen section images are preprocessed, segmented, labeled in accordance with the major organs and tissues of human beings, and reconstructed into three-dimensional (3D) models in parallel on high performance computing clusters (HPC). Some visualization software for 2D atlas and 3D mod-els are developed based on the new dataset with high resolution (0.1mm×0.1mm×0.2mm). In order to share, release and popularize the above work, a website (***) is online. The dataset is one of the most important parts in the national information database and the medical infrastructure.

关键词：数学人体解剖学可视化分辨率