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Spatio-temporal Variation of Soil Respiration and Its Driving Factors in Semi-arid Regions of North China

Chinese Geographical Science 2018年第1期28卷 12-24页

作者： zeng xinhua SONG Yigang ZHANG Wanjun HE Shengbing College of Environmental Science and Engineering Shanghai Jiaotong University Key Laboratory of Agricultural Water Resources Center for Agricultural Resources Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Shanghai Chenshan Plant Science Research Center Chinese Academy of Sciences

Soil respiration （SR） is the second-largest flux in ecosystem carbon cycling. Due to the large spatio-temporal variability of environmental factors, SR varied among different vegetation types, thereby impeding accurate estimation of CO2 emissions via SR. However, studies on spatio-temporal variation of SR are still scarce for semi-arid regions of North China. In this study, we conducted 12-month SR measurements in six land-use types, including two secondary forests （Populus tomentosa （PT） and Robinia pseudoacacia （RP））, three artificial plantations （Armeniaca sibirica （AS）, Punica granatum （PG） and Ziziphusjujuba （Z J）） and one natural grassland （GR）, to quantify spatio-temporal variation of SR and distinguish its controlling factors. Results indicated that SR exhibited distinct sea- sonal patterns for the six sites. Soil respiration peaked in August 2012 and bottomed in April 2013. The temporal coefficient of variation （CI0 of SR for the six sites ranged from 76.98% to 94.08%, while the spatial CV of SR ranged from 20.28% to 72.97% across the 12-month measurement. Soil temperature and soil moisture were the major controlling factors of temporal variation of SR in the six sites, while spatial variation in SR was mainly caused by the differences in soil total nitrogen （STN）, soil organic carbon （SOC）, net photosynthesis rate, and fine root biomass. Our results show that the annual average SR and Q10 （temperature sensitivity of soil respira- tion） values tended to decrease from secondary forests and grassland to plantations, indicating that the conversion of natural ecosystems to man-made ecosystems may reduce CO2 emissions and SR temperature sensitivity. Due to the high spatio-temporal variation of SR in our study area, care should be taken when converting secondary forests and grassland to plantations from the point view of accurately quantifying C02 emissions via SR at regional scales.

关键词： soil respiration spatio-temporal variation substrate availability temperature sensitivity global carbon cycle North China

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Application of Brain-Computer-Interface in Awareness Detection Using Machine Learning Methods

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China Communications 2022年第6期19卷 279-291页

作者： Kaiqiang Feng Weilong Lin Feng Wu Chengxin Pang Liang Song Yijia Wu Rong Cao Huiliang Shang xinhua zeng Academy for Engineering and Technology Fudan UniversityShanghai 200433China School of Information Science and Technology Fudan UniversityShanghai 200433China Department of Cardiology Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai 200437China Electronics and Information Engineering Shanghai University of Electric PowerShanghai 200090China

The awareness detection in patients with disorders of consciousness currently relies on behavioral observations and CRS-R tests,however,the misdiagnosis rates have been relatively *** this study,we applied brain-computer interface(BCI)to awareness detection with a passive auditory stimulation paradigm.12 subjects with normal hearing were invited to collect electroencephalogram(EEG)based on a BCI communication system,in which EEG signals are transmitted *** necessary preprocessing,RBF-SVM and EEGNet were used for algorithm realization and *** a single subject,RBF-SVM can distinguish his(her)name stimuli awareness with classification accuracies ranging from 60-95%.EEGNet was used to learn all subjects'data and improved accuracy to 78.04%for characteristics finding and model ***,we completed the supplementary analysis work from the time domain and time-frequency *** study applied BCI communication to human awareness detection,proposed a passive auditory paradigm,and proved the effectiveness,which could be an inspiration for brain,mental or physical diseases diagnosis and detection.

关键词： brain-computer interface EEG awareness detection machine learning deep learning

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Carbon Storage Capacity of Different Plantation Types Under Sandstorm Source Control Program in Hebei Province, China

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Chinese Geographical Science 2014年第4期24卷 454-460页

作者： SHEN Huitao ZHANG Wanjun YANG Xue LIU Xiuping CAO Jiansheng zeng xinhua ZHAO Xin CHEN Xuexun ZHANG Wenxi Key Laboratory of Agricultural Water Resources Hebei Key Laboratory of Agricultural Water-Saving Center for Agricultural Resources Research Institute of Genetics and Developmental BiologyChinese Academy of Sciences Agriculture School Kunming University Forestry Bureau of Aohan Banner Forestry Bureau of Hexigten Banner

Afforestation and reforestation are effective and ecological ways of mitigating elevated atmospheric carbon dioxide(CO2) concentration and increasing carbon(C) storage in terrestrial ecosystems. In this study, we measured the above-ground(tree, herbaceous plants and litter) and below-ground(root and soil) C storage in an aspen plantation(Populus davidiana) monoculture(PD), a larch plantation(Larix pincipis-rupprechtii) monoculture(LP), a pine plantation(Pinus tabulaeformis) monoculture(PT), a larch and birch mixed plantation(L. pincipis-rupprechtii and Betula platyphlla mixed)(MLB), and an apricot plantation(Armeniaca sibirica) monoculture(AS) under the Desertification Combating Program in Hebei Province, the northern China. The objective was to assess the effect of afforestation species on ecosystem C pools of different plantation types. Results showed that C storage of LP stand(258.0 Mg/ha) and MLB(163.4 Mg/ha) were significantly higher than the C storage in PD(45.5 Mg/ha), PT(58.9 Mg/ha) and AS(49.4 Mg/ha), respectively. Soil C was the main carbon pool of the ecosystem C storage in the five plantation stands, ranging from 31.4 Mg/ha to 232.5 Mg/ha, which accounted for 69.0%–90.1% of the total ecosystem C storage. The C storage in tree layer was about 5.2%–23.2% of ecosystem C storage. The herbaceous plants and litter layers contained 1.0%–6.0% and 1.5%–3.3% of ecosystem C storage, respectively. Our results suggest that tree species should be incorporated to accurately develop regional C budget of afforestation program, and also imply that substantial differences in ecosystem C stocks among plantation types can facilitate decision making on C management.

关键词： carbon content carbon storage forestry program tree species

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Change of Soil Organic Carbon after Cropland Afforestation in ′Beijing-Tianjin Sandstorm Source Control′ Program Area in China

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Chinese Geographical Science 2014年第4期24卷 461-470页

作者： zeng xinhua ZHANG Wanjun LIU Xiuping CAO Jiansheng SHEN Huitao ZHAO Xin ZHANG Nan-nan BAI Yuru Yi Mei Key Laboratory of Agricultural Water Resources Hebei Key Laboratory of Agricultural Water-Saving Center for Agricultural Resources Research Institute of Genetics and Developmental BiologyChinese Academy of Sciences University of Chinese Academy of Sciences Forestry Research Institute of Chifeng City

Land use change is one of the major factors that affect soil organic carbon （SOC） variation and global carbon balance. However, the effects of land use change on SOC are always variable. In this study, using a series of paired-field experiments, we estimated the effects of revegetation types and environmental conditions on SOC stock and vertical distribution after replacement of cropland with poplar （Populus tomentosa） and korshinsk peashrub （Caragana korshinskt＇i） in three climate regions （Chifeng City, Fengning City and Datong City of the ＇Beijing-Tianjin Sandstorm Source Control＇（BTSSC） program area. The results show that SOC sequestration rate ranges from 0.15 Mg/（ha-yr） to 3.76 Mg/（ha-yr） in the soil layer of 0-100 cm in early stage after cropland afforestation in the BTSSC program area. The SOC accumulation rates are the highest in Fengning for both the two vegetation types. Compared to C. korshinskii, P tomentosa has greater effects on SOC accumulation in the three climate regions, but significantly greater effect only appears in Datong. The SOC density increases by 20%-111% and 15%-59% for P. tomentosa and 9%-63% and 0-73% for C. korshinskii in the 0-20 cm and 20-100 cm soil layers, respectively. Our results indicate that cropland afforestation not only affects SOC stock in the topsoil, but also has some effects on subsoil carbon. However, the effect of cropland afforestation on SOC accumulation varied with climate regions and revegetation types. Considering the large area of revegetation and relatively high SOC accumulation rate, SOC sequestration in the BTSSC program should contribute significantly to decrease the CO2 concentration in the atmosphere.

关键词： soil organic carbon (SOC) cropland afforestation soil profile carbon sequestration Beijing-Tianjin Sandstorm SourceControl program

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RNA-Seq identifies genes associated with aberrant meiotic prophase I of a novel TE5A genic male sterility in Brassica napus

RNA-Seq identifies genes associated with aberrant meiotic pr...

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2016年全国植物生物学大会

作者： Xiaohong Yan xinhua zeng Gang Wu Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture

Genic male sterility(GMS),as a promising pollination control system,has already been extensively utilized for hybrid rapeseed production. TE5A is a novel thermo-sensitive dominant GMS line in Brassica napus. Analysis of paraffin-cross sections of anthers and TEM showed that the male gamete development was arrested at meiosis prophase I,eventually,meiotic PMCs were degenerated,leaving an empty locule,and no pollen grains were generated. Chromosome spreads and FISH showed that homologous chromosomes could not pair,synapse,condense and form bivalents in TE5A. Ed U uptake of S-phase meiocytes revealed that TE5A mutant could accomplish DNA replication. We then analyzed the transcriptome differences between young floral buds of sterile plants and its near-isogenic fertile plants. RNA-Seq analysis identified 3,841 differentially expressed genes(DEGs),some of which were associated with homologous chromosome behavior and cell cycle control during meiosis. These DEGs represented a set of potential candidate genes associated with GMS in the TE5A. Dynamic expression changes of candidate DEGs were then detected at different developmental stages of anthers. The present study provided a global assessment of the differences between GMS sterile plants and its near-isogenic fertile plants,as well as identified new fertility-associated genes and elucidated the mechanisms of GMS.

关键词： genic male sterility RNA-Seq prophase I meiotic cycle meiosis

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Numerical Simulation of Deforming Manufacture Process for Automobile Bevel Gear Blank

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武汉理工大学学报 2006年第S1期28卷 143-146页

作者： WANG Zewu,zeng Shuqin,YANG xinhua,WANG Cheng (Institute of Engineering Computation and Simulation,Huazhong University of Science and Technology, Wuhan 430074,China) Institute of Engineering Computation and Simulation Huazhong University of Science and TechnologyWuhan 430074China

Aiming at solving the difficulties of deforming manufacture of the automobile rear axle bevel gear blank,the paper presented to adopt the numerical simulation method to study the rolling process of the bevel gear blank instead of the traditional expensive trail-and-error method. A three-dimensional simulation model of the Φ500 bevel gear blank radial ring rolling machine was firstly created based on the general dynamic explicit finite element code ANSYS/LS-DYNA,and then realized the virtual simulation of the entire rolling process of the bevel gear blank within a producing cycle. The simulation results displayed the real-time blank enlarging and fleck generating as well as the stress,strain and displacement contours,which are in good agreements with the real ring rolling process. It is concluded that this numerical simulation method is feasible and can be used to guide the practical rolling process of the bevel gear blank as well as other profile-shaped annular blanks.

关键词： bevel gear blank ring rolling numerical simulation finite element LS-DYNA

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审判委员会讨论决定权的法教义学阐释

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法学杂志 2019年第11期40卷 130-140页

作者：曾新华对外经济贸易大学法学院

法教义学以规范为对象、以解释为方法、以体系为目标,应成为当下刑事诉讼法学研究方法的重要分支。审判委员会"讨论决定重大、疑难、复杂案件的法律适用"应基于法教义学立场和方法进行解释。"讨论决定"时实行的民主集中制应解释为合议制... 详细信息

法教义学以规范为对象、以解释为方法、以体系为目标,应成为当下刑事诉讼法学研究方法的重要分支。审判委员会"讨论决定重大、疑难、复杂案件的法律适用"应基于法教义学立场和方法进行解释。"讨论决定"时实行的民主集中制应解释为合议制;无论从《刑事诉讼法》还是《人民法院组织法》体系上看,审判委员会讨论决定刑事案件时,辩护律师要求列席的应当准许。"重大、疑难、复杂案件"是不确定法律概念,只能采取价值补充法进行解释。"法律适用"改革是保留审判委员会制度下最具现实合理性的方案,法律适用与事实认定问题的区分应以是否需要进行证据调查为标准。

关键词：审判委员会法教义学讨论决定重大疑难复杂案件法律适用

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New highly specific anti-BnASY polyclonal antibody prepara-tion and application in immunofluorescence assay

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Oil Crop Science 2016年第4期1卷 58-64页

作者： Keqi Li Rong Yuan Xiaohong Yan xinhua zeng Ruixing Guo Gang Wu Key Laboratory of Biology and Genetic improvement of Oil crops Ministry of Agriculture Oil Crops Research Institute Chi-nese Academy of Agricultural Sciences Wuhan China

ASY （asynaptic） is a synaptonemal complex （SC） related protein in plant mei- osis. Some ASYs in plants have been cloned and functional determined. ASY in Brassica napus has not been sequenced and functional certificated. Here, we first cloned ASY gene in Brassica napus （BnASY） and inserted it into vector pET-32a for expression fusion protein BnASY-6His in Escherichia coli BL21 （DE3）. Purified fusion protein was used to produce polyclonal antibody. Specificity and application of polyclonal antibody was examined by Western blot and immunofluorescence assay. Results showed that BnASY-6His recombi-nant protein and anti-BnASY antibody were successfully obtained. Polyclonal anti-BnASY antibody had high specificity. Results of immunofluorescence showed that ASY signals appeared at leptotene stage, faded away gradually at pachytene stage and almost disap-peared at diplonema stage. In this study, highly specific polyclonal antibody was success-fully acquired and used for immunofluorescence assay in plant cells. It will contribute to functional research of ASY in B. *** thank Zhigang Li for providing pET-32a vector and Escherichia coli BL21 pLysS strains.

关键词： Brassica napus BnASY polyclonal antibody immunofluorescence

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Crysformer:An attention-based graph neural network for properties prediction of crystals

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Chinese Physics B 2023年第9期32卷 15-20页

作者：王田陈家辉滕婧史金钢曾新华 Hichem Snoussi Institute of Artificial Intelligence SKLSDEBeihang UniversityBeijing 100191China Zhongguancun Laboratory Beijing 100191China School of Aeronautic Science and Engineering Beihang UniversityBeijing 100191China School of Control and Computer Engineering North China Electric Power UniversityBeijing 102206China School of Software Engineering Xi'an Jiaotong UniversityXi'an 710049China Academy for Engineering and Technology Fudan UniversityShanghai 200433China Charles Delaunay Institute University of Technology of TroyesTroyes Cedex 10004France

We present a novel approach for the prediction of crystal material properties that is distinct from the computationally complex and expensive density functional theory(DFT)-based ***,we utilize an attention-based graph neural network that yields high-accuracy *** approach employs two attention mechanisms that allow for message passing on the crystal graphs,which in turn enable the model to selectively attend to pertinent atoms and their local environments,thereby improving *** conduct comprehensive experiments to validate our approach,which demonstrates that our method surpasses existing methods in terms of predictive *** results suggest that deep learning,particularly attention-based networks,holds significant promise for predicting crystal material properties,with implications for material discovery and the refined intelligent systems.

关键词： deep learning property prediction crystal attention networks

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Copy number and zygosity determination of transgenic rapeseed by droplet digital PCR

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Oil Crop Science 2017年第2期2卷 84-94页

作者： Yuhua Wu Jun Li Xiaying Li Jingang Liang Yunjing Li xinhua zeng Gang Wu Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture Oil Crops Research InstituteChinese Academy of Agricultural Sciences Wuhan China Science and Technoloty Center of Ministry of Agriculture China

Establishing an accurate and rapid method for copy number and zygosity determination can accelerate genetic engineering research process. In this study, droplet digital PCR (ddPCR), an emerging DNA absolute quantification technology, was used to identify single-copy homozygous transgenic lines from a batch of T0 transgenic rapeseed harboring 11 exogenous elements. Copy number of exogenous gene was evaluated in T0 generation based on calculated ratio between transgene and reference CruA gene, single-copy transformants were selected for selfing followed by subsequent zygosity analysis. Single-copy homozygous transgenic plants were successfully screened out in T1 generation by *** analysis with T2 seedlings verified that identification results of ddPCR were accurate and reliable. This study provides a novel rapid and accurate method for copy number and zygosity determination in transgenic rapeseed which overcomes disadvantages of traditional Southern analysis and recently developed real-time quantitative method.

关键词： droplet digital PCR (ddPCR) copy number zygosity homozygotes transgenic rapeseed

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