检索结果-南通市图书馆

Regulation of cytokinin biosynthesis using PtRD26pro-IPT module improves drought tolerance through PtARR10-PtyUC4/5-mediated reactive oxygen species removal in Populus

引用

Journal of Integrative Plant Biology 2022年第3期64卷 771-786页

作者： Hou-Ling Wang Qi yang Shuya Tan Ting Wang yi zhang yanli yang Weilun yin Xinli Xia Hongwei guo zhonghai Li National Engineering Laboratory for Tree Breeding College of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing100083 China Department of Biology Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education InstitutesSouthern University of Science and Technology(SUSTech)Shenzhen518055 China

Drought is a critical environmental factor which constrains plant survival and *** engineering provides a credible strategy to improve drought tolerance of ***,we generated transgenic poplar lines expressing the isopentenyl transferase gene(IPT)under the driver of Pt RD26 promoter(PtRD26 pro-IPT).Pt RD26 is a senescence and drought-inducible NAC transcription *** RD26 pro-IPT plants displayed multiple phenotypes,including improved growth and drought *** analysis revealed that auxin biosynthesis pathway was activated in the PtRD26 pro-IPT plants,leading to an increase in auxin *** analysis revealed that ARABIDOPSIS RESPONSE REGULATOR10(PtARR10),one of the type-B ARR transcription factors in the cytokinin pathway,was induced in PtRD26 pro-IPT plants and directly regulated the transcripts of yUCCA4(Pt yUC4)and yUCCA5(PtyUC5),two enzymes in the auxin biosynthesis *** of PtyUC4 enhanced drought tolerance,while simultaneous silencing of PtyUC4/5 evidently attenuated the drought tolerance of Pt RD26 pro-IPT ***,Pt yUC4/5 displayed a conserved thioredoxin reductase activity that is required for drought tolerance by deterring reactive oxygen species *** work reveals the molecular basis of cytokinin and auxin interactions in response to environmental stresses,and shed light on the improvement of drought tolerance without a growth penalty in trees by molecular breeding.

关键词： auxin cytokinin drought tolerance leaf senescence PtARR PtyUC ROS

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

APPLICATIONS OF CFD TECHNIQUE IN THE DESIGN AND FLOW ANALySIS OF IMPLANTABLE AXIAL FLOW BLOOD PUMP

引用

Journal of Hydrodynamics 2010年第4期22卷 518-525页

作者： FAN Hui-min HONG Fang-wen zHANG guo-ping yE Liang LIU zhong-min Shanghai East Hospital Affiliated to Tongji University Shanghai 200120 China China Ship Scientific Research Center Wuxi 214082 China

Based on established numerical methods and hydrodynamic performance testing facilities,CFD technique are applied to improve the design of the implantable axial flow blood pump and the flow analysis.（1） Applying brushless machine magnet steel,reducing its thickness while increasing the length,the flow channel＇s cross-section is increased,with no space connection between the large and the small rotators,and with a cone transition segment from the bearing to the principal axis,the flow is made *** rotating speed is lowered by 1000 rpm-1200 rpm under the same flow rate and pressure head,and thus the hemolysis can be avoided.（2） Different outlet stator guiding vanes are selected for the same blood pump for analyses of hydrodynamic performances and flow *** excellent design not only can regulate the rotating flow field into an axial one,reduce the circulation loss and improve the pump efficiency,but also can avoid backflow,vortex and secondary flow at the pump outlet,and thus the thrombus can be prevented.（3） The calf live tests show that some residual clots exist at the inner wall of the outlet connection bends,which are analyzed and explained by CFD techniques and the corresponding improvements are *** results are verified by hydrodynamic performance tests and PIV flow field tests,and consistent conclusions are obtained.

关键词： axial flow blood pump CFD PIV

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Multi-channel micro neural probe fabricated with SOI

引用

Science China(Technological Sciences) 2009年第5期52卷 1187-1190页

作者： PEI WeiHua zHU Lin WANG ShuJing guo Kai TANG Jun zHANG Xu LU Lin GAO ShangKai CHEN HongDa Department of Biomedical Engineering Tsinghua UniversityBeijing 100084China State Key Laboratory on Integrated Optoelectronics Institute of SemiconductorsChinese Academy of SciencesBeijing 100083China

Silicon-on-insulator(SOI) substrate is widely used in micro-electro-mechanical systems(MEMS).With the buried oxide layer of SOI acting as an etching stop,silicon based micro neural probe can be fabri-cated with improved uniformity and manufacturability.A seven-record-site neural probe was formed by inductive-coupled plasma(ICP) dry etching of an SOI *** thickness of the probe is 15 μ*** shaft of the probe has dimensions of 3 mm×100 μm×15 μm with typical area of the record site of 78.5 μ*** impedance of the record site was measured *** typical impedance characteris-tics of the record sites are around 2 MΩ at 1 *** performance of the neural probe in-vivo was tested on anesthetic *** recorded neural spike was typically around 140 μ*** from individual site could exceed 700 μ*** average signal noise ratio was 7 or more.

关键词： micro neural probe SOI impedance recording

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

盘形滚刀对TBM破岩效率的影响参数分析及优化设计

引用

福建理工大学学报 2024年第1期22卷 8-15页

作者：周亚来詹金武邵顺安郑晋溪刘国武玉娇福建理工大学土木工程学院福建福州350118 福建省建筑工程质量检测中心有限公司福建福州350118

为探究盘形滚刀形式对TBM破岩效率的影响,依托福州滨海快线工程项目,利用ABAQUS创建盘形滚刀破岩模型,通过优化单刃滚刀结构参数,分析多滚刀破岩方式对掘进效率的影响。结果表明:刃宽和贯入度对滚刀破岩的影响最大,随着刃宽和贯入度增加... 详细信息

为探究盘形滚刀形式对TBM破岩效率的影响,依托福州滨海快线工程项目,利用ABAQUS创建盘形滚刀破岩模型,通过优化单刃滚刀结构参数,分析多滚刀破岩方式对掘进效率的影响。结果表明:刃宽和贯入度对滚刀破岩的影响最大,随着刃宽和贯入度增加,滚刀受到的滚动力、垂直力和破岩体积逐渐增大,并在刃宽20 mm、贯入度10 mm时,达到最佳破岩效果;双滚刀下采用同步破岩的效率要明显高于顺次破岩,其最优刀间距为60 mm;随着四滚刀回转半径增加,滚刀受到的切向力、垂直力和合力均增大,侧向力呈现先增大后减小的趋势,在回转半径350 mm处达到最大。研究成果可为福州滨海快线项目TBM滚刀选型及施工方案优化设计提供依据。

关键词： ABAQUS数值模拟滚刀结构参数刀间距回转破岩

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Spliceosomal GTPase Eftud2 regulates microglial activation and polarization

引用

Neural Regeneration Research 2023年第4期18卷 856-862页

作者： guo-Chao yang yuan Shi Chao-Nan Fan ying Li Meng-Qi yuan Jie Pei yan Wu Hai-Tao Wu School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina Department of Neurobiology Beijing Institute of Basic Medical SciencesBeijingChina Institute of Neuroscience Hengyang Medical CollegeUniversity of South ChinaHengyangHunan ProvinceChina School of Basic Medicine Anhui Medical UniversityHefeiAnhui ProvinceChina Key Laboratory of Neuroregeneration Co-innovation Center of NeuroregenerationNantong UniversityNantongJiangsu ProvinceChina

Elongation factor Tu GTP binding domain protein 2(Eftud2)is a spliceosomal GTPase that serves as an innate immune modulator restricting virus *** are the resident innate immune cells and the key players of immune response in the central nervous ***,the role of Eftud2 in microglia has not been *** this study,we performed immunofluorescent staining and western blot assay and found that Eftud2 was upregulated in microglia of a 5xFAD transgenic mouse model of Alzheimer’s ***,we generated an inducible microglia-specific Eftud2 conditional knockout mouse line(CX3CR1-CreER;Eftud2^(f/f) cKO)via Cre/loxP recombination and found that Eftud2 deficiency resulted in abnormal proliferation and promoted anti-inflammatory phenotype activation of ***,we knocked down Eftud2 in BV2 microglia with siRNA specifically targeting Eftud2 and found that Eftud2-mediated regulation of microglial proinflammatory/anti-inflammatory phenotype activation in response to inflammation might be dependent on the NF-κB signaling *** findings suggest that Eftud2 plays a key role in regulating microglial polarization and homeostasis possibly through the NF-κB signaling pathway.

关键词： Alzheimer’s disease anti-inflammatory phenotype BV2 Eftud2 inflammation lipopolysaccharide microglia nuclear factor-kappaB proinflammatory phenotype spliceosomal GTPase

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Microstructure and texture evolution of Mg–7y–1Nd–0.5zr alloy sheets with different rolling temperatures

引用

Rare Metals 2020年第11期39卷 1273-1278页

作者： Jing-Bao Liu Kui zhang Jing-Tao Han Xing-Gang Li yong-Jun Li Ming-Long Ma Jia-Wei yuan guo-Liang Shi State Key Laboratory for Fabrication and Processing of Nonferrous Metals Beijing General Research Institute for Nonferrous MetalsBeijing100088China School of Materials Science and Engineering University of Science and Technology BeijingBeijing100083China

The extruded Mg-7y-1Nd-0.5zr(wt%)alloy were performed to the same strain hot rolling with different *** microstructure and texture evolution of the sheets were investigated by optical microscopy(OM),scanning electron microscopy(SEM),transmission electron microscopy(TEM),X-ray diffraction(XRD),and electron back-scattered diffraction(EBSD).The results indicate that the microstructure becomes homogeneous after hot rolling process and precipitated phase distributes at grain boundaries along rolling *** the increase in rolling temperature,the grains of sheet grow *** sheet rolling at 400℃is composed of recrystallization grains,the necklace of the precipitated phase in the grain boundaries and excessive *** structure of necklace of the precipitated phase is fcc structure with lattice constant of a=0.75 *** rolling temperatures increasing from 400 to 450℃,the content of recrystallized grains in volume fraction with relatively random orientations increases *** with the rolling process at 400℃,the amount of precipitated phases is reduced at the grain boundary,and the precipitated phase begins to appear in the grain interior when rolling at 450℃.The structure of the precipitated phase is fcc with lattice constant of a=2.22 *** recrystallization grains begin to grow in the rolling process at 500℃.The basal texture is obviously produced during the rolling process at 400 and 450℃;however,the basal texture is weak in the rolling process at 500℃.

关键词： Rare earth magnesium alloy Rolling Texture Precipitated phase

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Two homologous INDOLE-3-ACETAIVHDE (IAM) HyDROLASE genes are required for the auxin effects of IAM in Arabidopsis

引用

Journal of Genetics and Genomics 2020年第3期47卷 157-165页

作者： yangbin Gao Xinhua Dai yuki Aoi yumiko Takebayashi Liping yang Xiaorui guo Qiwei zeng Hanchuanzhi yu Hiroyuki Kasahara yunde zhao Section of Cell and Developmental Biology University of California San DiegoLa JollaCA92093-0116USA RIKEN Center for Sustainable Resource Science Kanagawa230-0045Japan School of Life Sciences Jilin Normal UniversitySiping136000China Key Laboratory of Plant Ecology Northeast Forestry UniversityHarbin150040China State Key Laboratory of Silkworm Genome Biology Institute of Sericulture and Systems BiologySouthwest UniversityChongqing400716China Institute of Global Innovation Research Tokyo University of Agriculture and TechnologyTokyo183-8509Japan Department of Bioregulation and Biointeraction Graduate School of AgricultureTokyo University of Agriculture and TechnologyFuchu183-8509Japan

Indole-3-acetamide(IAM)is the first confirmed auxin biosynthetic intermediate in some plant pathogenic *** applied IAM or production of IAM by overexpressing the bacterial iaaM gene in Arabidopsis causes auxin overproduction ***,it is still inconclusive whether plants use IAM as a key precursor for auxin ***,we reported the isolation IAM HyDROLASE 1(IAMH1)gene in Arabidopsis from a forward genetic screen for IAM-insensitive mutants that display normal auxin ***1 has a close homolog named IAMH2 that is located right next to IAMH1on chromosomeⅣin *** generated iamh1 iamh2 double mutants using our CRISPR/Cas9gene editing *** showed that disruption of the IAMH genes rendered Arabidopsis plants resistant to IAM treatments and also suppressed the iaaM overexpression phenotypes,suggesting that IAMH1 and IAMH2 are the main enzymes responsible for converting IAM into indole-3-acetic acid(IAA)in *** iamh double mutants did not display obvious developmental defects,indicating that IAM does not play a major role in auxin biosynthesis under normal growth *** findings provide a solid foundation for clarifying the roles of IAM in auxin biosynthesis and plant development.

关键词： Auxin Auxin biosynthesis Indole-3-acetamide Arabidopsis CRISPR IAMH1 IAMH2

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Decellularized extracellular matrix particle-based biomaterials for cartilage repair applications

引用

Journal of Materials Science & Technology 2023年第29期160卷 194-203页

作者： Peng guo Nan Jiang Carina Mini Gregor Miklosic Songsong zhu Andrea J.Vernengo Matteo D’Este Sibylle Grad Mauro Alini zhen Li AO Research Institute Davos Davos 7270Switzerland Department of Spine Surgery The Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhou 510655China State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Disease and West China Hospital of Stomatology Sichuan UniversityChengdu 610041China Institute for Biomechanics ETH ZürichZürich 8093Switzerland

Cartilage Decellularized ExtraCellular Matrix(dECM)materials have shown promising cartilage regenera-tion capacity due to their chondrogenic ***,the limited retention of ECM components and the reduced integrity of functional ECM molecules during traditional decellularization processes im-pair the biomimicry of these *** current study aims to fabricate biomimetic materials con-taining decellularized cartilage particles that have an intact molecular structure and native composition as biomaterial inks and hydrogels for cartilage *** this,we established a novel two-fraction de-cellularization strategy for the preparation of reconstituted dECM(rdECM)particles by mixing the two-fraction components,as well as a one-fraction decellularization strategy for the preparation of biomimetic dECM(bdECM)*** acid-tyramine(THA)hydrogels containing rdECM or bdECM particles were produced and characterized via rheological test,swelling and stability evaluation,and compression *** results showed that our novel decellularization strategies preserved intact proteoglycans and collagen at a higher retention rate with adequate DNA removal compared to traditional methods of *** addition of rdECM or bdECM particles significantly increased the shear moduli of the THA bioinks while preserving their shear-thinning *** particle-embedded THA hydrogels also achieved long-term stability with a swelling ratio of 70%and high retention of glycosaminoglycans and collagen after long-term incubation,while rdECM particle-embedded THA hydrogels showed unsat-isfactory stability as self-standing *** to pure THA hydrogels,the addition of bdECM particles significantly enhanced the compression *** summary,our decellularization methods are successful in the retention of functional and intact cartilage components with high *** rdECM and bdECM particles can be supplemented in THA bioinks for biomimet

关键词： Cartilage tissue engineering Decellularized extracellular matrix Proteoglycan integrity Biomimetic hydrogels Biomaterial Bioink

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phytochrome B interacts with SWC6 and ARP6 to regulate H2A.z deposition and photomorphogensis in Arabidopsis

引用

Journal of Integrative Plant Biology 2021年第6期63卷 1133-1146页

作者： Xuxu Wei Wanting Wang Peng Xu Wenxiu Wang Tongtong guo Shuang Kou Minqing Liu yake Niu Hong-Quan yang zhilei Mao Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China School of Life Sciences Fudan UniversityShanghai 200438China

Light serves as a crucial environmental cue which modulates plant growth and development, and which is controlled by multiple photoreceptors including the primary red light photoreceptor,phytochrome B(phyB). The signaling mechanism of phyB involves direct interactions with a group of basic helix-loop-helix(bHLH) transcription factors, PHyTOCHROME-INTERACTING FACTORS(PIFs), and the negative regulators of photomorphogenesis, COP1 and SPAs. H2 A.z is an evolutionarily conserved H2 A variant which plays essential roles in transcriptional regulation. The replacement of H2 A with H2 A.z is catalyzed by the SWR1 complex. Here, we show that the Pfr form of phyB physically interacts with the SWR1 complex subunits SWC6 and ARP6. phyB and ARP6 coregulate numerous genes in the same direction,some of which are associated with auxin biosynthesis and response including yUC9, which encodes a rate-limiting enzyme in the tryptophandependent auxin biosynthesis pathway. Moreover,phyB and Hy5/HyH act to inhibit hypocotyl elongation partially through repression of auxin biosynthesis. Based on our findings and previous studies, we propose that phyB promotes H2 A.z deposition at yUC9 to inhibit its expression through direct phyB-SWC6/ARP6 interactions,leading to repression of auxin biosynthesis, and thus inhibition of hypocotyl elongation in red light.

关键词： ARP6 H2A.z hypocotyl elongation photomorphogenesis phytochrome B red light SWC6

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Discovery of alkaloids from the leaves of Isatis indigotica Fortune with neuroprotective activity

引用

Chinese Journal of Natural Medicines 2021年第9期19卷 680-685页

作者： XI yu-Fei LOU Li-Li HAN Feng-ying WANG Xiao-Bo HUANG Xiao-Xiao yAO guo-Dong SONG Shao-Jiang Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research&Development Liaoning ProvinceSchool of Traditional Chinese Materia MedicaShenyang Pharmaceutical UniversityShenyang 110016China Chinese People's Liberation Army Logistics Support Force No.967 Hospital Dalian 116000China

Seven alkaloids including five undescribed ones(1 a/1 b,2,3 and 5)were obtained from the leaves of Isatis indigotica *** structures were established by extensive spectroscopic *** absolute configurations of compounds 1 a,1 b,3 and 5 were determined by comparison of the experimental and calculated electronic circular dichroism(ECD)***,the neuroprotective effects of all the isolates against H_(2)O_(2)-induced injury in SH-Sy5 y cells were evaluated in vitro by MTT ***,Annexin V-FITC/PI double staining was performed,while the activities of antioxidant enzymes(SOD,CAT and GSH-Px)for compounds 1 a and 1 b were measured.

关键词： Isatis indigotica Alkaloids Neuroprotective effects Structure elucidation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：