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Journal of Wuhan University of Technology(Materials Science) 2024年第4期39卷 945-953页

作者：王俊凯李来时 LIU Feng wang yuzheng 吴玉胜 School of Material Science and Engineering Shenyang University of TechnologyShenyang 110870China

A method to promote aluminum hydroxide crystal growth through pickling Al(OH)_(3)as seed in the ammonia system was proposed to overcome these *** experimental results show that,under the conditions of pickling time of 15 min,the acid concentration of 10%,the addition of 70 g/L pickling-Al(OH)_(3)seed,and the coarse granular Al(OH)_(3)products(d0.5=85.667)can be *** characterization results show that the phase of the product is gibbsite,consistent with the ***,the steps and ledges can be formed on pickling Al(OH)_(3)seed surface under the ammonia system,effectively promoting crystal *** crystal growth,the roughness of the crystal surface was first increased and then decreased,and the lamellar structure was deposited on the crystal seed *** final particles are approximately round,the surface is compact and *** growth of the product is surface reaction *** addition,the content of the AlO_(6)unit is increased and contributed to Al(OH)_(3)crystal growth.

关键词： reactive crystallization coarse-grained Al(OH)_(3) crystal seed crystal growth process

CRISPR/Cas systems for the detection of nucleic acid and nonnucleic acid targets

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Nano Research 2023年第7期16卷 9940-9953页

作者： Weiran Su Junru Li Chen Ji Congshuo Chen yuzheng wang Huili Dai Fengqin Li Peifeng Liu State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer InstituteRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200032China Central Laboratory Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Micro-Nano Research and Diagnosis Center Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China

Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas)systems are becoming powerful tools for disease biomarkers *** to the specific recognition,cis-cleavage and nonspecific trans-cleavage capabilities,CRISPR/Cas systems have implemented the detection of nucleic acid targets(DNA and RNA)as well as non-nucleic acid targets(e.g.,proteins,exosomes,cells,and small molecules).In this review,we first summarize the principles and characteristics of various CRISPR/Cas systems,including CRISPR/Cas9,Cas12,Cas13 and Cas14 ***,various types of applications of CRISPR/Cas systems used in detecting nucleic and non-nucleic acid targets are introduced ***,the prospects and challenges of their applications in biosensing are discussed.

关键词： clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas) detection nucleic acid targets non-nucleic acid targets

Characteristics and influence factors of NO2 exchange flux between the atmosphere and P. nigra

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Journal of Environmental Sciences 2019年第10期31卷 155-165页

作者： Chun Chen yuzheng wang Yuanyuan Zhang Chengtang Liu Xiaoxiu Lun Yujing Mu Chenglong Zhang Junfeng Liu Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China University of Chinese Academy of Sciences Beijing 100049 China Center for Excellence in Urban Atmospheric Environment Institute of Urban Environment Chinese Academy of Sciences Xiamen 361021China College of Environmental Science & Engineering Beijing Forest University Beijing 100083 China

Nitrogen dioxide(NO2)is an important substance in atmospheric photochemical processes and can also be absorbed by plants.N02 fluxes between the atmosphere and *** seedlings were investigated by a double dynamic chambers method in Beijing from June 15to September 3,*** range of N02 exchange fluxes between *** seedlings and the atmosphere was from-14.6 to 0.8 nmol/(***)(the positive data represent N02 emissior from trees,while the negative values indicate absorption).Under ambient concentrations the mean NO2 flux during the fast-growing stage(Jun.15-Aug.4)was-3.0 nmol/(m2·sec)greater than the flux of-1.5 nmol/(m2-sec)during the later growth stage(Aug.8-Sept.3)The daily exchange fluxes of NO2 obviously *** fluxes were largest in the morning and decreased gradually over ***,the N02 fluxes were larger undei high light intensities than under low light intensities during the whole growth *** effects of temperature on N02 fluxes were different under two growth *** NO2exchange fluxes were larger in a range of temperatures close to 44℃in the fast-growing stage,whereas there were no evident differences in N02 exchange fluxes under widel differing temperatures in the later growth *** polluted conditions,the uptake ability of N02 was ***,the compensation point of N02 was 5.6 ppb ir the fast-growing stage,whereas it was 1.4 ppb in the later growth *** depositior velocities of NO2 were between 0.3 and 2.4 mm/sec.

关键词： Nitrogen dioxide Exchange flux P.nigra Compensation point

Evaluation of offline sampling for atmospheric C3-C11 non-methane hydrocarbons

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Journal of Environmental Sciences 2022年第3期34卷 132-140页

作者： Zhiguo Liu Chengtang Liu Zhouming He Yujing Mu Chenglong Zhang Yuanyuan Zhang Pengfei Liu yuzheng wang Junfeng Liu Research Center for Eco-Environmental Sciences Chinese Academy of SciencesBeijing 100085China Center for Excellence in Urban Atmospheric Environment Institute of Regional EnvironmentChinese Academy of SciencesXiamen 361021China University of Chinese Academy of Sciences Beijing 100049China Clear Science&Technology Co. LtdBeijing 100029China

The concentration variation of C3-C11 non-methane hydrocarbons(NMHCs)collected in several types of commercial flexible bags and adsorption tubes was systematically inves-tigated using a gas chromatography-flame ionization detector(GC-FID)*** per-centage loss of each NMHC in the polyvinyl fluoride(PVF)bags was less than 5%during a 7-hr storage period;significant NMHCs loss was detected in aluminum foil composite film and fluorinated ethylene propylene *** thermal desorption efficiency of NMHCs for adsorption tubes filled Carbopack B and Carboxen1000 sorbents was greater than 95%at 300℃,and the loss of NMHCs in the adsorption tubes during 20-days storage at 4℃was less than 8%.The thermal desorption efficiency for C11 NMHCs in the adsorption tube filled with Carbograph 1 and Carbosieve SⅢabsorbents was less than 40%at 300℃,and pyrolysis of the absorbents at 330℃interfered significantly with the measurements of some *** loss of alkenes was significant when NMHCs were sampled by cryo-enrichment at-90℃in the presence of O3 for the online NMHC measurements,and negligible for enrichment us-ing adsorption tubes at 25℃.Although O_(3)scrubbers have been widely used to eliminate the influence of O_(3)on NMHC measurements,the loss of NMHCs with carbon numbers greater than 8 was more than 10%.Therefore,PVF bags and adsorption tubes filled Carbopack B and Carboxen1000 sorbents were recommended for the sampling of atmospheric NMHCs.

关键词： Non-methane hydrocarbons(NMHCs) Gas chromatography-flame ionization detector(GC-FID) Offline sampling Adsorption tubes Ozone scrubber

Dyskinesia is Closely Associated with Synchronization of Theta Oscillatory Activity Between the Substantia Nigra Pars Reticulata and Motor Cortex in the Off L-dopa State in Rats

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Neuroscience Bulletin 2021年第3期37卷 323-338页

作者： Jiazhi Chen Qiang wang Nanxiang Li Shujie Huang Min Li Junbin Cai yuzheng wang Huantao Wen Siyuan Lv Ning wang Jinyan wang Fei Luo wangming Zhang The National Key Clinic Specialty The Engineering Technology Research Center of the Ministry of Education of ChinaGuangdong Provincial Key Laboratory on Brain Function Repair and RegenerationDepartment of NeurosurgeryZhujiang HospitalSouthern Medical UniversityGuangzhou 510282China Key Laboratory of Mental Health Institute of PsychologyChinese Academy of SciencesBeijing 100101China Movement Disorders and Neuromodulation Unit Department for NeurologyCharite´-University Medicine Berlin10117 BerlinGermany

Excessive theta(θ)frequency oscillation and synchronization in the basal ganglia(BG)has been reported in elderly parkinsonian patients and animal models of levodopa(L-dopa)-induced dyskinesia(LID),particularly theθoscillation recorded during periods when L-dopa is withdrawn(the off L-dopa state).To gain insight into processes underlying this activity,we explored the relationship between primary motor cortex(M1)oscillatory activity and BG output in *** recorded local field potentials in the substantia nigra pars reticulata(SNr)and M1 of awake,inattentive resting rats before and after L-dopa priming in Sham control,Parkinson disease model,and LID model *** found that chronic L-dopa increasedθsynchronization and information flow between the SNr and M1 in off L-dopa state LID rats,with a SNr-to-M1 flow *** with the on state,θoscillational activity(θsynchronization and informationflow)during the off state were more closely associated with abnormal involuntary *** findings indicate thatθoscillation in M1 may be consequent to abnormal synchronous discharges in the BG and support the notion that M1θoscillation may participate in the induction of dyskinesia.

关键词： Parkinson's disease Dyskinesia Substantia nigra pars reticulata Primary motor cortex Synchronization

Activity maintenance of the excised branches and a case study of NO_2 exchange between the atmosphere and *** branches

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Journal of Environmental Sciences 2019年第6期31卷 316-326页

作者： Chun Chen yuzheng wang Yuanyuan Zhang Xiaoxiu Lun Chengtang Liu Yujing Mu Chenglong Zhang Pengfei Liu Chaoyang Xue Min Song Can Ye Junfeng Liu Research Center for Eco-Environmental Sciences Chinese Academy of Sciences University of Chinese Academy of Sciences Center for Excellence in Urban Atmospheric Environment Institute of Urban Environment Chinese Academy of Sciences College of Environmental Science & Engineering Beijing Forest University

The efficient maintenance of the activity of excised branches is the powerful guarantee to accurately determine gas exchange flux between the detached branches of tall trees and the atmosphere. In this study, the net photosynthetic rate(NPR) of the excised branches and branches in situ were measured simultaneously by using two photosynthetic instruments to characterize the activity of the excised branches of Phyllostachys nigra. The ratio of normalized NPR of excised branches to NPR in situ was used to assess the photosynthetic activity of detached branches. Based on photosynthetic activity, an optimal hydroponics protocol for maintaining activity of excised P. nigra branches was presented:1/8 times the concentration of Gamborg B5 vitamin mixture with p H = 6. Under the best cultivation protocol, photosynthetic activity of excised P. nigra branches could be maintained more than 90% within 6 hr in the light intensity range of 200–2000 μmol/(m2·sec) and temperature range of 13.4–28.7°C. The nitrogen dioxide(NO2) flux differences between in situ and in vitro branches and the atmosphere were compared using double dynamic *** on the maintenance method of excised branches, the NO2 exchange flux between the excised P. nigra branches and the atmosphere(from-1.01 to-2.72 nmol/(m2·sec) was basically consistent with between the branches in situ and the atmosphere(from-1.12 to-3.16 nmol/(m2 sec)) within 6 hr. Therefore, this study provided a feasible protocol for in vitro measurement of gas exchange between tall trees and the atmosphere for a period of time.

关键词： Excised branches Activity maintenance NO2 exchange flux Hydroponics Dynamic chamber

Synthesis and Antimicrobial Activity of Boron-doped Titania Nano-materials

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Chinese Journal of Chemical Engineering 2014年第4期22卷 474-479页

作者：王昱征薛向欣杨合 Institute of Metallurgical Resources and Environmental Engineering Northeastern University Liaoning Key Laboratory of Metallurgical Resources Recycling Science Liaoning Engineering and Technology Research Center of Boron Resources Comprehensive Utilization Liaoning Provincial Universities Key Laboratory of Boron Resources Ecological Utilization Technology and Boron Materials

Antibacterial activity of boron-doped TiO2(B/TiO2) nano-materials under visible light irradiation and in the dark was investigated. A simple sol-gel method was used to synthesize TiO2 nano-materials. X-ray diffraction pattern of B/TiO2 nano-materials represents the diffraction peaks relating to the crystal planes of TiO2(anatase and rutile). X-ray photoelectron spectroscopy result shows that part of boron ions incorporates into TiO2 lattice to form a possible chemical environment like Ti O B and the rest exist in the form of B2O3. The study on antibacterial effect of B/TiO2 nano-materials on fungal Candida albicans(ATCC10231), Gram-negative Escherichia coli(ATCC25922) and Gram-positive Staphylococcus aureus(ATCC6538) shows that the antibacterial action is more significant on Candida albicans than on Escherichia coli and Staphylococcus aureus. Under visible light irradiation, the antibacterial activity is superior to that in the dark.

关键词： boron doping titania antimicrobial activity

1992-2016年中国肾癌死亡趋势的年龄-时期-队列分析

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中华流行病学杂志 2021年第3期42卷 508-512页

作者：王昱政胡樱济南铁路疾病预防控制中心 250001 武汉大学健康学院 430071

目的了解1992-2016年中国肾癌死亡率的现状和长期趋势。方法死亡率数据来自2016年全球疾病负担数据。采用由美国国立卫生研究院提供的年龄-时期-队列模型在线分析工具,对1992-2016年中国肾癌死亡数据进行分析。结果1992-2016年,中国男... 详细信息

目的了解1992-2016年中国肾癌死亡率的现状和长期趋势。方法死亡率数据来自2016年全球疾病负担数据。采用由美国国立卫生研究院提供的年龄-时期-队列模型在线分析工具,对1992-2016年中国肾癌死亡数据进行分析。结果1992-2016年,中国男性和女性的肾癌粗死亡率、标化死亡率总体均呈增加趋势,增幅均较大。男性肾癌死亡率平均年度变化百分比(AAPC)的估计值为2.85%(95%CI:2.68%~3.02%),女性肾癌死亡率AAPC的估计值为1.25%(95%CI:1.04%~1.45%)。1992-2016年男性和女性的局部漂移值均>0(均P<0.05)。就年龄效应而言,矫正了队列效应和时期效应后,自15岁起,男性和女性肾癌的死亡风险随年龄呈指数上升。从15~19岁至75~79岁年龄组的各生命阶段,每提高1个5岁年龄组,肾癌死亡的RR值男性为1.85,女性为1.59。两种性别的队列和时期效应均具有统计学意义(均P<0.05),总体及各年龄组的AAPC均有统计学意义(均P<0.05)。结论肾癌死亡率增幅较大,对于肾癌防治应当引起足够的重视。

关键词：肾癌死亡率年龄-时期-队列分析

Analysis for the Defect of Chromatogram in 35kv Oil-immersed Electric Reactor

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Energy and Power Engineering 2013年第4期5卷 838-841页

作者： Yongdong Li Qingda Meng Zheyuan Zhao Po Yang yuzheng wang Maintenance of northern Hebei Province Electric Power Co. Ltd Substation maintenance center Beijing China

A defect of chromatogram in a 35kV oil-immersed three-phase one-piece electric reactor in a 500kV substation。The initial analysis suggests that the defect is caused by the loose connection of the iron core magnet shield (magnet shield layer of iron core or earthling top). Examination of winding DC resistance, DGA and other relevant examinations were carried out to investigate the defect reasons., which is demonstrated by the dismantlement. In-depth study is taken and corresponding prevention measures are put forward in the paper.

关键词： Oil-immersed Electric Reactor Chromatogram Defect Magnet Shield Layer Partial Discharge

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长期护理保险对家庭代际转移支付的影响

金融评论 2024年第1期16卷 130-153,156页

作者：王煜正杨再贵王乃琦中央财经大学中国精算研究院

长期护理保险的家庭代际资源分配效应在优化收入分配格局的进程中发挥着重要作用。基于中国健康与养老追踪调查(CHARLS)数据、中国长期护理保险试点政策数据库(CLIP)和《中国城市统计年鉴》数据,本文使用渐进双重差分法和事件研究法检... 详细信息

长期护理保险的家庭代际资源分配效应在优化收入分配格局的进程中发挥着重要作用。基于中国健康与养老追踪调查(CHARLS)数据、中国长期护理保险试点政策数据库(CLIP)和《中国城市统计年鉴》数据,本文使用渐进双重差分法和事件研究法检验长期护理保险对家庭代际转移支付的影响。研究发现:长期护理保险试点实施带来了家庭代际向下转移支付的增加,而家庭代际向上转移支付没有明显变化;长期护理保险通过改善失能老人的健康状况和经济状况从而增加家庭代际向下转移支付,且家庭代际向下转移支付是由失能老人的利他动机驱动的;长期护理保险试点实施使参保家庭的经济资源增长。上述结果揭示出完善长期护理保险制度设计及优化家庭收入分配结构的必要性。

关键词：长期护理保险代际转移支付家庭经济