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Three-dimensional reconstruction of light microscopy image sections： present and future

Frontiers of Medicine 2015年第1期9卷 30-45页

作者： yuzhen wang rui xu gaoxing luo jun wu Institute of Burn Research Southwest Hospital State Key Laboratory of Trauma Burns and Combined lnjury Chongqing Key Laboratory for Diseases Proteomics the Third Military Medical University Chongqing 400038 China

Three-dimensional （3D） image reconstruction technologies can reveal previously hidden microstruc-tures in human tissue. However, the lack of ideal, non-destructive cross-sectional imaging techniques is still a problem. Despite some drawbacks, histological sectioning remains one of the most powerful methods for accurate high-resolution representation of tissue structures. Computer technologies can produce 3D representations of interesting human tissue and organs that have been serial-sectioned, dyed or stained, imaged, and segmented for 3D visualization. 3D reconstruction also has great potential in the fields of tissue engineering and 3D printing. This article outlines the most common methods for 3D tissue section reconstruction. We describe the most important academic concepts in this field, and provide critical explanations and comparisons. We also note key steps in the reconstruction procedures, and highlight recent progress in the development of new reconstruction methods.

关键词： microtomy 3D imaging computer-assisted image processing 3D printing tissue scaffold

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A systematic and quantitative method for wound-dressing evaluation

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Burns & Trauma 2015年第2期3卷 114-121页

作者： Xiaorong Zhang rui xu Xiaohong Hu gaoxing luo jun wu Weifeng He Chongqing Key Laboratory for Disease Proteomics State Key Lab of TraumaBurns and Combined InjuryInstitute of Burn ResearchSouthwest HospitalThe Third Military Medical UniversityChongqing 400038China

Background:For patients with skin defects such as burns,wound dressing plays important roles in protecting the wound.Before a novel wound dressing is applied to a patient,a series of tests should be performed to ensure its safety and efficacy.Different types of animal wound-healing models have been used to study the bio-function of different wound dressings;however,a systematic way to evaluate the effect of a wound dressing on wound healing and cutaneous regeneration is lacking.Methods:In the study presented here,full-thickness wound models were established in mice,and a systematic way to quantitatively analyze the wound-healing process and the histological results is described.Results:It was found that the rate of wound healing in the tested wound dressing(TWD)group was higher than that in the control group,and the re-epithelialization and the formation of granulation tissue were enhanced when the TWD was applied.Meanwhile,the inflammatory response was attenuated in the TWD group,and more mature and better aligned collagen fibers in the healed wound tissue was found in the TWD group compared with that in the control group.Conclusions:A systematic,quantitative way to analyze the effect of a wound dressing on wound healing was established.And it might be helpful for the design of wound dressing in the future.

关键词： Wound dressing Evaluation Wound model

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壳聚糖/藻酸盐纳米膜在促进小鼠全层损伤皮肤创面愈合中的作用

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第三军医大学学报 2015年第21期37卷 2109-2114页

作者：孔易徐瑞邢梦秋贺伟峰罗高兴吴军第三军医大学西南医院全军烧伤研究所创伤烧伤与复合伤国家重点实验室重庆400038 加拿大曼尼托巴大学机械工程学院

目的评价复合生物材料壳聚糖/藻酸盐纳米膜作为组织工程的细胞支架材料,通过促进小鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)在其表面的粘附、迁移、增殖及分化,从而加速皮肤创面修复的效果。方法实验动物为BALB/c小鼠,8周龄,... 详细信息

目的评价复合生物材料壳聚糖/藻酸盐纳米膜作为组织工程的细胞支架材料,通过促进小鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)在其表面的粘附、迁移、增殖及分化,从而加速皮肤创面修复的效果。方法实验动物为BALB/c小鼠,8周龄,于各小鼠背部制备皮肤缺损创面模型。实验先分2组,实验组小鼠背部创面以壳聚糖/藻酸盐纳米膜覆盖,对照组不予该纳米膜覆盖创面,直接粘贴手术贴巾,并分别围绕创缘皮下注射等量绿色荧光蛋白(green fluorescent protein,GFP)标记的骨髓间充质干细胞(GFP-MSCs),在术后进行小动物活体成像观察绿色荧光蛋白信号强度,用流式细胞仪检测阳性GFP-MSCs比例,并取创面新生组织行冰冻切片观察GFP-MSCs生长情况;再分别设置空白对照组、纳米膜组、MSCs注射组和纳米膜+MSCs注射组,观察术后各组小鼠创面愈合情况。结果实验组小鼠术后第7天的创面GFP信号强度[(25.97±6.98)×104]明显高于对照组[(6.00±2.84)×104,P<0.05];流式细胞仪检测创面GFP-MSCs水平,术后第5天实验组阳性率[(66.78±7.00)%]明显高于对照组[(33.58±3.62)%,P<0.01],第7天实验组阳性率[(53.03±3.15)%]明显高于对照组[(34.20±7.98)%,P<0.01];术后第14天小鼠新生组织中GFP-MSCs密度实验组[(101.00±15.51)个/视野]明显高于对照组[(25.25±5.07)个/视野,P<0.05];创面愈合观察发现纳米膜+MSCs注射组创面愈合速度快于其他3组(P<0.05)。结论壳聚糖/藻酸盐纳米膜用于皮肤创面,可促进MSCs在体内粘附、生长、分化、迁移,进而促进皮肤组织修复,加速创面愈合,是一种良好的细胞支架材料。

关键词：壳聚糖/藻酸盐纳米膜组织工程细胞支架创面愈合

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