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The epidermis-specific cyclin CYCP3;1 is involved in the excess brassinosteroid signaling-inhibited root meristem cell division

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Journal of Integrative Plant Biology 2020年第11期62卷 1674-1687页

作者： yuxiao chen Shiyong Sun Xuelu Wang State Key Laboratory of Genetic Engineering Department of GeneticsSchool of Life SciencesFudan UniversityShanghai 200433China State Key Laboratory of Crop Stress Adaptation and Improvement Henan UniversityKaifeng 475001China

Cell division is precisely regulated and highly tissue-specific;studies have suggested that diverse signals in the epidermis,especially the epidermal brassinosteroids(BRs),can regulate root ***,the underlying molecular mechanisms that integrate hormonal cues such as BR signaling with other endogenous,tissue-specific developmental programs to regulate epidermal cell proliferation remain *** this study,we used molecular and biochemical approaches,microscopic imaging and genetic analysis to investigate the function and mechanisms of a P-type cyclin in root growth *** found that CYCP3;1,specifically expressed in the root meristem epidermis and lateral root cap,can regulate meristem cell *** analyses and biochemical studies demonstrated that CYCP3;1 promotes cell division at the G2-M duration by associating and activating cyclin-dependent kinase B2-1(CDKB2;i).Furthermore,we found that CYCP^;1 expression was inhibited by BR signaling through BRI1-EMS-SUPPRESSOR1(BES1),a positive downstream transcription factor in the BR signaling *** findings not only provide a mechanism of how root epidermal-specific regulators modulate root growth,but also reveal why the excess of BRs or enhanced BR signaling inhibits cell division in the meristem to negatively regulate root growth.

关键词： inhibited endogenous excess

Photoinduced self-stability mechanism of CdS photocatalyst:The dependence of photocorrosion and H_(2)-evolution performance

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Journal of Materials Science & Technology 2022年第26期121卷 19-27页

作者： yuxiao chen Wei Zhong Feng chen Ping Wang Jiajie Fan Huogen Yu School of Chemistry Chemical Engineering and Life Sciencesand State Key Laboratory of Silicate Materials for ArchitecturesWuhan University of TechnologyWuhan 430070China Laboratory of Solar Fuel Faculty of Materials Science and ChemistryChina University of GeosciencesWuhan 430070China School of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450002China

CdS photocorrosion is one of the most important factors that greatly affect the photocatalytic H_(2)-production rate and long-time ***,the reported works about CdS photocorrosion are mainly focused on the surface oxidation by photogenerated holes,while the possible reduction of lattice Cd^(2+) by photogenerated electrons is usually *** this work,the lattice Cd^(2+) reduction by photogenerated electrons during CdS photocorrosion were carefully investigated to reveal its potential effect on the microstructure change and photocatalytic H_(2)-production performance of CdS photocatalyst based on the two typical Na_(2)S-Na_(2)SO_(3) and lactic acid H_(2)-evolution *** was found that many isolated metallic Cd nanoparticles(5–50 nm)were produced on the CdS surface in the Na_(2)S-Na_(2)SO_(3) system,causing its serious destroy of CdS surface and a gradually decreased photocatalytic activity,while only a metallic Cd layer(2-3 nm)is homogeneously coated on the CdS surface in the lactic acid system,leading to an increased H-evolution *** fact,once a certain amount of metallic Cd was produced on the CdS surface,the resulting CdS-Cd composites can present a stable photocatalytic H_(2)-production activity and excellent stability for the final CdS-Cd ***,a photoinduced self-stability mechanism of CdS photocatalyst has been proposed,namely,the spontaneously produced metallic Cd contributes to the transformation of unstable CdS into stable CdS-Cd structure,with the simultaneous realization of final stable H_(2)-evolution performance.

关键词： Photocatalysis H_(2)evolution CdS Photocorrosion Self-stability

Identification and Characterization of Peptides Mimicking the Epitopes of Metalloprotease of Schistosoma Japonicum

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Cellular & Molecular Immunology 2005年第3期2卷 219-223页

作者： Lianfei Tang yuxiao chen Linqian Wang Shunke Zhang Xianfang Zeng Xinyuan Yi Department of Parasitology Xiangya School of Medicine Central SouthUniversity Changsha 410008 China Technology Centre Hunan Entry-Exit Inspection and Quarantine BureauChangsha China

In an attempt to isolate and characterize peptides mimicking epitopes of metalloprotease and explore their immunological protection against Schistosoma japonicum （S. japonicum）, polyclonal anti-metalloprotease sera was prepared to screen a 12-mer random peptide library to isolate phages binding specially to antisera IgG. Then, phage ELISA, animal immunization, DNA sequencing, Western blotting and enzymatic activity neutralizing analysis were used to characterize the selected phage clones. All of ten randomly picked clones were shown to be positive. Five peptides of different amino acid sequences deduced from DNA sequences were obtained and two of them （peptides 2 and 3） could induce significant reduction （31.0% and 31.8%, respectively） in worm burden and high reduction （52.6% and 54.9%, respectively） in liver eggs per gram （LEPG）, while, unexpectedly, others （peptides 1, 4 and 5） could not elicit enough protection against infection of S. japonicum. Peptides 2 and 3 could be recognized by S. japonicum infected mouse sera （IMS） and could elicit neutralizing Abs. The results show that peptides 2 and 3 are antigenic and immunogenic. They are true mimics of epitopes of metalloprotease and useful as novel vaccine candidates against ***. Cellular ＆ Molecular Immunology. 2005;2（3）：219-223.

关键词： phage displayed peptide library epitope Schistosomajaponicum metalloprotease

Petrogenesis of Aoyougou high-silica adakite in the North Qilian orogen,NW China:Evidence for decompression melting of oceanic slab

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Chinese Science Bulletin 2012年第22期57卷 2289-2301页

作者： yuxiao chen XiaoHong Xia ShuGuang Song Peking Univ Sch Earth & Space Sci MOE Key Lab Orogen Belts & Crustal Evolut Beijing 100871 Peoples R China

A trondhjemitic body occurs in the Aoyougou area,the western part of the North Qilian *** is geochemically characterized by high SiO 2,Na 2 O (high Na/K),elevated Sr/Y and (La/Yb) N,positive Sr anomaly,relatively enriched large ion lithophile elements (LILEs) and light rare earth elements (LREEs),and depleted Nb,Ta,Ti,resembling the high-silica *** U-Pb SHRIMP dating yields a weighted mean age of 438±3 *** age is significantly younger than eclogitization ages of 460-490 Ma in the North Qilian orogen,suggesting that formation of the adakite postdates the subduction of oceanic crust in association with closure of the ancient Qilian ***-rock Sr and Nd isotopic analyses give initial ratios of I Sr =0.7044 0.7047 and Nd (t)=3.0 4.1,indicating that they are derived from partial melting of the juvenile oceanic *** view of the tectonic evolution of the North Qilian orogen,the high-silica adakite was probably derived from decompression melting of the exhumed eclogite at the depth of ～60 km.

关键词：北祁连造山带大洋地壳部分熔融埃达克岩稀土元素地球化学特征高硅中国西北减压

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Abscisic Acid Signaling Inhibits Bmssinosteroid Signaling through Dampening the Dephosphorylation of BIN2 by ABI1 and ABI2

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Molecular Plant 2018年第2期11卷 315-325页

作者： Haijiao Wang Jie Tang Jing Liu Jin Hu Jingjing Liu yuxiao chen Zhenying Cai Xuelu Wang Center of Integrative Biology College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 China State Key Laboratory of Genetic Engineering Department of Genetics School of Life Sciences Fudan University Shanghai 200433 China

Abscisic acid （ABA） and brassinosteroid （BR） antagonistically regulate many aspects of plant growth and development. Previous physiological studies have revealed that the inhibition of BR signaling by ABA is largely dependent on ABI1 and ABI2. However, the genetic and molecular basis of how ABI1 and ABI2 are involved in inhibiting BR signaling remains unclear. Although it is known that in the BR signaling pathway the ABA-BR crosstalk occurs in the downstream of BR receptor complex but upstream of BIN2 kinase, a negative regulator of BR signaling, the component that acts as the hub to directly mediate their crosstalk remains a big mystery. Here, we found that ABI1 and ABI2 interact with and dephosphorylate BIN2 to regu- late its activity toward the phosphorylation of BESl. Byin vitro mimicking ABA signal transduction, we found that ABA can promote BIN2 phosphorylation by inhibiting ABI2 through ABA receptors. RNA-sequencing analysis further demonstrated that ABA inhibits BR signaling through the ABA primary signaling components, including its receptors and ABI2, and that ABA and GSK3s co-regulate a common set of stress- responsive genes. Because BIN2 can interact with and phosphorylate SnRK2s to activate its kinase activity, our study also reveals there is a module of PP2Cs--BIN2-SnRK2s in the ABA signaling pathway. Collectively, these findings provide significant insights into how plants balance growth and survival by coordinately regu- lating the growth-promoting signaling pathway and stress responses under abiotic stresses.

关键词： Brassinosteroids Abscisic acid GSK3 cross-talk Phosphatase

Piezoelectric hydrogel for treatment of periodontitis through bioenergetic activation

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Bioactive Materials 2024年第5期35卷 346-361页

作者： Xin Liu Xingyi Wan Baiyan Sui Quanhong Hu Zhirong Liu Tingting Ding Jiao Zhao yuxiao chen Zhong Lin Wang Linlin Li Department of Dental Materials Shanghai Biomaterials Research&Testing CenterShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineCollege of StomatologyShanghai Jiao Tong UniversityNational Center for StomatologyNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of StomatologyShanghai200011PR China Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of SciencesBeijing101400PR China School of Nanoscience and Technology University of Chinese Academy of SciencesBeijing100049PR China

The impaired differentiation ability of resident cells and disordered immune microenvironment in periodontitis pose a huge challenge for bone ***,we construct a piezoelectric hydrogel to rescue the impaired osteogenic capability and rebuild the regenerative immune microenvironment through bioenergetic *** local mechanical stress,the piezoelectric hydrogel generated piezopotential that initiates osteogenic differentiation of inflammatory periodontal ligament stem cells(PDLSCs)via modulating energy metabolism and promoting adenosine triphosphate(ATP)***,it also reshapes an anti-inflammatory and pro-regenerative niche through switching M1 macrophages to the M2 *** synergy of tilapia gelatin and piezoelectric stimulation enhances in situ regeneration in periodontal inflammatory defects of *** findings pave a new pathway for treating periodontitis and other immune-related bone defects through piezoelectric stimulation-enabled energy metabolism modulation and immunomodulation.

关键词： Piezoelectric hydrogel Mitochondrial bioenergetics Periodontitis Bone regeneration Macrophage polarization

Exponential Dichotomies and Homoclinic Orbits from Heteroclinic Cycles

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American Journal of Computational Mathematics 2012年第2期2卷 106-113页

作者： Tiejun chen Yu Xiang yuxiao chen Yiyang Medical College Hunan Pro of China Yiyang China

In this paper, we investigate the homoclinic bifurcations from a heteroclinic cycle by using exponential dichotomies. We give a Melnikov—type condition assuring the existence of homoclinic orbits form heteroclinic cy... 详细信息

关键词： Exponential Dichotomies Homoclinic Orbits Heteroclinic Cycle Melnikov Function

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聚丙烯基3D打印材料的设计及制备研究进展

合成树脂及塑料 2024年第2期41卷 66-71页

作者：陈雨潇郑州商学院河南巩义451200

综述了3D打印用聚丙烯(PP)专利技术最新进展。通过多种改性途径,可以将PP改性为3D打印用免喷涂材料、低收缩材料、防翘曲材料、高韧性材料、荧光材料、交联材料、超低密度材料、增韧材料、耐辐照材料、可低温打印材料、中子屏蔽材料、... 详细信息

综述了3D打印用聚丙烯(PP)专利技术最新进展。通过多种改性途径,可以将PP改性为3D打印用免喷涂材料、低收缩材料、防翘曲材料、高韧性材料、荧光材料、交联材料、超低密度材料、增韧材料、耐辐照材料、可低温打印材料、中子屏蔽材料、纳米材料、丙烯酸树脂改性材料、超高相对分子质量聚乙烯改性材料及溶剂法材料,进而得到具有不同性能、满足不同行业需求的3D打印材料。

关键词： 3D打印聚丙烯熔融沉积成型专利

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木木美术馆入口改造,北京,中国

世界建筑 2017年第12期 56-61页

作者： chen yuxiao 直向建筑

木木美术馆入口改造是一个位于北京798艺术区的城市更新项目。这个曾经的废弃工业厂房,在2014年就被转换成了一个美术馆。2016年年初,美术馆的运营者希望对这个房子的外观和入口空间再做改造,以完善到访者的观展和活动体验,并且提升美... 详细信息

木木美术馆入口改造是一个位于北京798艺术区的城市更新项目。这个曾经的废弃工业厂房,在2014年就被转换成了一个美术馆。2016年年初,美术馆的运营者希望对这个房子的外观和入口空间再做改造,以完善到访者的观展和活动体验,并且提升美术馆公共形象的识别度。我们设计的起点,是怎么看待城市的演变。过去20年,中国的城市化经历了一个迅猛发展的过程,大量旧的房子、街道、街区,甚至地形地貌.

关键词：入口空间美术馆改造北京中国城市更新工业厂房地形地貌