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Brassinosteroids modulate nitrogen physiological response and promote nitrogen uptake in maize(Zea mays L.)

The Crop Journal 2022年第1期10卷 166-176页

作者： Jiapeng Xing Yubin Wang Qingqing Yao yushi zhang Mingcai zhang Zhaohu Li State Key Laboratory of Plant Physiology and Biochemistry College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Crop Research Institute Shandong Academy of Agricultural SciencesJinan 250100ShandongChina

Brassinosteroids(BRs)are steroid hormones that function in plant growth and development and response to environmental stresses and nutrient ***,few studies have investigated the effect of BRs in modulating the physiological response to nitrogen(N)supply in *** the present study,BR signalingdeficient mutant zmbri1-RNAi lines and exogenous application of 2,4-epibrassinolide(e BL)were used to study the role of BRs in the regulation of physiological response in maize seedlings supplied with *** application of e BL increased primary root length and plant biomass,but zmbri1 plants showed shorter primary roots and less plant biomass than wild-type plants under low N(LN)and normal N(NN)*** induced the expression of the BR signaling-associated genes Zm DWF4,Zm CPD,Zm DET2,and Zm BZR1 and the production of longer primary roots than *** of Zm BRI1 weakened the biological effects of LN-induced primary root elongation.e BL treatment increased N accumulation in shoots and roots of maize seedlings exposed to LN or NN ***,zmbri1 plants showed lower N accumulation in shoots and roots than wild-type *** with reduced N accumulation,zmbri1 plants showed lower NO3-fluxes and^(15)NO_(3)^(-)*** expression of nitrate transporter(NRT)genes(Zm NPF6.4,Zm NPF6.6,Zm NRT2.1,Zm NRT2.2)was lower in zmbri1 than in wild-type roots,but e BL treatments up-regulated the transcript expression of NRT ***,BRs modulated N physiological response and regulated the transcript expression of NRT genes to promote N uptake in maize.

关键词： Brassinosteroids Nitrogen uptake Nitrate transporter gene Root architecture Maize

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Ethylene accelerates maize leaf senescence in response to nitrogen deficiency by regulating chlorophyll metabolism and autophagy

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The Crop Journal 2024年第5期12卷 1391-1403页

作者： Jiapeng Xing Ying Feng yushi zhang Yubin Wang Zhaohu Li Mingcai zhang State Key Laboratory of Plant Environmental Resilience College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China College of Agriculture Northeast Agricultural UniversityHarbin 150030HeilongjiangChina

Leaf senescence is an orderly and highly coordinated process,and finely regulated by ethylene and nitrogen(N),ultimately affecting grain yield and nitrogen-use efficiency(NUE).However,the underlying regulatory mechanisms on the crosstalk between ethylene-and N-regulated leaf senescence remain a mystery in *** this study,ethylene biosynthesis gene ZmACS7 overexpressing(OE-ZmACS7)plants were used to study the role of ethylene regulating leaf senescence in response to N deficiency,and they exhibited the premature leaf senescence accompanied by increased ethylene release,decreased chlorophyll content and F_v/F_m ratio,and accelerated chloroplast ***,we investigated the dynamics changes of transcriptome reprogramming underlying ethylene-accelerated leaf senescence in response to N *** differentially expressed genes(DEGs)involved in chlorophyll biosynthesis were significantly down-regulated,while DEGs involved in chlorophyll degradation and autophagy processes were significantly up-regulated,especially in OE-ZmACS7 plants in response to N deficiency.A gene regulatory network(GRN)was predicted during ethylene-accelerated leaf senescence in response to N *** transcription factors(TFs)ZmHSF4,Zmb HLH106,and ZmEREB147 were identified as the key regulatory genes,which targeted chlorophyll biosynthesis gene ZmLES22,chlorophyll degradation gene ZmNYC1,and autophagy-related gene ZmATG5,***,ethylene signaling key genes might be located upstream of these TFs,generating the signaling cascade networks during ethylene-accelerated leaf senescence in response to N ***,these findings improve our molecular knowledge of ethylene-accelerated maize leaf senescence in response to N deficiency,which is promising to improve NUE by manipulating the progress of leaf senescence in maize.

关键词： Ethylene Leaf senescence N deficiency Chlorophyll metabolism Autophagy Gene regulatory network

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Exogenous application of glycine betaine improved water use efficiency in winter wheat(Triticum aestivum L.) via modulating photosynthetic efficiency and antioxidative capacity under conventional and limited irrigation conditions

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The Crop Journal 2019年第5期7卷 635-650页

作者： Nazir Ahmed yushi zhang Ke Li Yuyi Zhou Mingcai zhang Zhaohu Li Engineering Research Center of Plant Growth Regulator Ministry of Education College of Agronomy and Biotechnology China Agricultural University

Improving water use efficiency(WUE)is an important subject in agricultural irrigation for alleviating the scarcity of water resources in semiarid regions of the North China ***,glycine betaine(GB)is one of the most effective compatible solutes synthesized naturally in plants for enhancing stress tolerance under abiotic stress,but little information is available on the involvement of GB in regulating crop WUE under field *** study was conducted to explore the role of exogenously applied GB in improving WUE and plant physiological and biochemical responses inwinterwheat subjected to conventional or limited irrigation during the 2015–2016 and 2016–2017 growing *** application of GB significantly enhanced antioxidant enzyme activities and reduced the accumulation ofmalondialdehyde and hydrogen peroxide under limited irrigation ***,GB-treated plantsmaintained higher leaf relative water content andmembrane stability,which led to higher chlorophyll content and gas exchange attributes for better intrinsic and instantaneouswater use efficiencies compared to control plants under limited irrigation ***-treated plants had higher indole-acetic acid and zeatin riboside levels but lower ABA levels compared to control plants under conventional and limited irrigation ***,GB enhanced the grain filling rate and duration,grain number per spike,and final grainweight,which resulted in higher grain yield compared to the ***,GB significantly improved the integrative and photosynthetic WUE under conventional and limited irrigation conditions,although GB treatment did not markedly affect total water *** results suggest the involvement of GB in improving WUEs in winter wheat by modulating hormonal balance,membrane stability,photosynthetic performance and antioxidant systems to maintain higher grain yield under conventional and limited irrigation conditions.

关键词： Winter wheat Glycine betaine Water use efficiency Limited irrigation Photosynthetic performance Antioxidant systems

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A new model for Doppler shift of C-band echoes backscattered from sea surface

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Acta Oceanologica Sinica 2023年第6期42卷 100-111页

作者： Jianbo Cui Yunhua Wang Yanmin zhang Huimin Li Wenzheng Jiang yushi zhang Xin Li College of Information Science and Engineering Ocean University of ChinaQingdao 266100China Qingdao National Laboratory for Marine Science and Technology Qingdao 266237China School of Marine Sciences Nanjing University of Information Science and TechnologyNanjing 210044China First Institute of Oceanography Ministry of Natural ResourcesQingdao 266061China National Key Laboratory of Electromagnetic Environment/China Research Institute of Radiowave Propagation Qingdao 266107China

Within the framework of the two-scale scattering model,the Doppler shift of C-band radar return signals from the nonlinear sea surface are numerically *** an analytical approximation method,the Bragg resonance scattering method cannot accurately describe the backscattering field from sea ***,in the twoscale scattering model,more accurate scattering coefficient(the normalized radar cross section,NRCS)evaluated by the C-band dual-polarized(HH/VV)empirical geophysical model function(CSAR model)is employed to replace the traditional Bragg NRCS to weight the Doppler *** numerical results indicate that there are obvious differences between the Doppler shift weighted by the CSAR NRCS and that weighted by the traditional Bragg *** hydrodynamic modulation of the large-scale waves is one of the important factors that affect the difference between the Doppler shift predicted in upwind and downwind *** the relaxation rate in the hydrodynamic modulation is set to be the angular frequency of the dominant water waves,the Doppler shift predicted by the numerical method can fit the results of the empirical model(C-band empirical geophysical model function,CDOP)well at moderate wind *** low wind condition,the comparison shows that the empirical CDOP model appears to overestimate the Doppler *** order to facilitate the application,at the end of this paper a semi-empirical CSAR-DOP model,which is a polynomial fitting formula,is developed for evaluating the Doppler shift of C-band signals from time varying sea surface.

关键词： Doppler shift microwave scattering numerical simulation

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Effects of AlCoCrFeNiTi high-entropy alloy on microstructure and mechanical properties of pure aluminum

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Journal of Materials Science & Technology 2020年第17期52卷 1-11页

作者： Qinglin Li Shang Zhao Xuepeng Bao yushi zhang Yuqian Zhu Chuangzao Wang Yefeng Lan Yuxin zhang Tiandong Xia School of Materials Science and Engineering Lanzhou University of TechnologyLanzhou 730050China State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals Lanzhou University of TechnologyLanzhou 730050China

A new kind of AlCoCrFeNiTi high-entropy alloy(HEA)as a grain refiner was prepared by vacuum arc *** this work,the effects of HEA(1.0 wt.%,2.0 wt.%and 3.0 wt.%)on the microstructure and mechanical properties of pure aluminum were *** microstructure was characterized and examined by scanning electron microscope(SEM),energy dispersive X-ray spectroscopy(EDS),X-ray diffraction(XRD),electron probe micro-analyzer(EPMA)and transmission electron microscopy(TEM)to indicate the refining abilities and mechanism of HEA on pure *** show that the addition of HEA refined both the macrostructure and microstructure of pure *** 1.0 wt.%HEA was added,most coarse columnar grains were refined into equiaxed crystals,and as the amount of HEA increased to 2.0 wt.%and3.0 wt.%,aluminum grains were further refined,and the grain boundaries were nearly ***,the morphology ofα-Al transformed from coarse columnar crystals to equiaxed grains,and the mean size ofα-Al grains decreased from 374μm to 27μ*** Al3Ti,Al3Ni,and nano-phase precipitated from the aluminum alloy with HEA in the *** typical rod-like nano-phases distributed interdendritic regions ofα-*** average length of nano-phases is 2568 nm,4372 nm,and 6907 nm and the average diameter is 112 nm,103 nm,and 92 nm when 1.0 wt.%,2.0 wt.%and 3.0 wt.%HEA were added to the pure aluminum,*** ultimate tensile strength(UTS)and yield strength(YS)were improved in all samples,whereas the elongation(El)was decreased with increasing HEA *** 3.0 wt.%HEA was added into the aluminum melt,the UTS was improved by 145.2%from62 MPa to 152 MPa,the YS was increased by 173.8%from 42 MPa to 115 MPa,and the El was decreased by 33.3%from 39%to 26%.

关键词： Pure aluminum High-entropy alloy α-Al phases Nano-phases Mechanical properties

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High resolution non-invasive intraocular pressure monitoring by use of graphene woven fabrics on contact lens

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Microsystems & Nanoengineering 2019年第1期5卷 273-280页

作者： yushi zhang Yufeng Chen Tianxing Man Dong Huang Xiao Li Hongwei Zhu Zhihong Li School of Information Engineering Minzu University of China100081 BeijingChina National Key Laboratory of Science and Technology on Micro/Nano Fabrication Institute of MicroelectronicsPeking University100871 BeijingChina Key Laboratory for Advanced Manufacturing by Materials Processing Technology Department of Mechanical EngineeringTsinghua University100084 BeijingChina Center for Nano and Micro Mechanics(CNMM) Tsinghua University100084 BeijingChina

Monitoring intracorporal pressures are important for health care and *** this work,a contact lens tonometer employing graphene woven fabrics(GWFs),which indicate great sensibility of resistance to strain,flexibility,stretchability,transparency,and biocompatibility,is proposed for real-time monitoring intraocular pressure(IOP)with high *** mechanical properties of the device during the deformation were analyzed,and the sensitivity of the fabricated device was tested on a mimic human *** vitro experiments on porcine eyes were executed to test the effectiveness of the *** change rate of resistance under different IOP was ***,the relationship between the current changes and IOP variation when keeping the voltage constant for different devices was *** contact lens tonometers with GWFs as high-resolution sensing element have shown a promising prospective to realize the low-cost disposable sensing contact lens with lower power.

关键词： graphene resolution transparency

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Toxicity study of ethanol extract from Oroxylumindicum(L.) Vent in rats

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Journal of Traditional Chinese Medicine 2018年第5期38卷 714-725页

作者： Yi Yan Zhao Yong Li Chunying zhang yushi Bin Yang Yuan Yalan Wang Lianmei Pan Chen Liang Aihua Institute of Chinese Materia Medica China Academy of Chinese Medical Science

OBJECTIVE: To evaluate the safety of Oroxylumindicum(L.) Vent extract administered for 26 weeks in Wistar ***: Oroxylumindicum(L.) Vent extract was administrated to male and female rats by gavage once daily at doses of 54, 225, and450 mg·kg-1·*** rats were sacrificed after administration for13 weeks and 26 weeks. Part of the rats in each group were allowed to recover for 4 weeks after 26-week administration. Systematic examinations including haematology, urology, blood biochemistry and histomorphology were performed at the end of13, 26 weeks of administration and 4 weeks of ***: No treatment related adverse effect shappened on rats' general status, body weight, food consumption, urinary index and histomorphologyexamination. Although during the administration,in some rats of extract's groups, the value of Red blood cell count(RBC), white blood cell counts(WBC), hemoglobin(HGB), haematocrit(HCT) and K iron were decreased, and biochemistry index,such as glucose(GLU), triglyceride(TG), alanine transaminase(ALT) and blood urea nitrogen(BUN)were increased, the above parameters were within the normal ranges and all returned to baseline after the drug stopping for 4 ***: The administration of Oroxylumindicum(L.) Vent extractat levers up to 450 mg/kg(equals to 75 times of clinical dose) is well toleratedfor both genders without significant toxicity within the administration duration in this study.

关键词： Oroxylumindicum (L.)Vent Safety Chronic toxicity Rats

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Evaluation of the chemical profile from four germplasms sources of Pruni Semen using UHPLC-LTQ-Orbitrap-MS and multivariate analyses

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Journal of Pharmaceutical Analysis 2022年第5期12卷 733-742页

作者： Zihan Zhao Yue Liu yushi zhang Zeyu Geng Rina Su Lipeng Zhou Chao Han Zhanjun Wang Shuangcheng Ma Weidong Li School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing102488China Institute of Forestry and Grassland Ecology Ningxia Academy of Agricultural and Forestry SciencesYinchuan750002China Institute for Control of Chinese Traditional Medicine and Ethnic Medicine National Institutes for Food and Drug ControlBeijing100050China

Pruni Semen,the seed of several unique Prunus plants,is a traditional purgative herbal *** determine the authentic sources of Pruni Semen,46 samples from four species were collected and *** compounds including multiflorin A(Mul A),a notable purative compound,were isolated and identified by chemical separation and nuclear magnetic resonance ***-six communal components were identified by ultra-high performance liquid chromatography with linear ion trap-quadrupole Orbitrap mass spectrometry,and acetyl flavonoid glycosides were recognized as characteristic *** flavonoids were distributed in the seed coat and cyanogenic glycosides in the *** on this,methods for identifying Pruni Semen from different sources were established using chemical fingerprinting,quantitative analysis of the eight principal compounds,hierarchical cluster analysis,principal component analysis,and orthogonal partial least squares discriminant *** results showed that the samples were divided into two categories:one is the small seeds from Prunus humilis(Ph)and Prunus japonica(Pj),and the other is the big seeds from Prunus pedunculata(Pp)and Prunus triloba(Pt).The average content of Mul A was 3.02,6.93,0.40,and 0.29 mg/g,while the average content of amygdalin was 18.5,17.7,31.5,and 30.9 mg/g in Ph,Pj,Pp,and Pt,*** the above information suggests that small seeds might be superior sources of Pruni *** is the first comprehensive report on the identification of chemical components in Pruni Semen from different species.

关键词： Pruni semen Acetyl flavonoids Amygdalin Multivariate analyses Authentic sources

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Potential chronic liver toxicity in rats orally administered an ethanol extract of Huangqin(Radix Scutellariae Baicalensis)

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Journal of Traditional Chinese Medicine 2018年第2期38卷 242-256页

作者： Yi Yan Zhao Yong Li Chunying zhang yushi Bin Yang Yuan Yalan Pan Chen Wang Lianmei Liang Aihua Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine Institute of Chinese Materia MedicaChina Academy of Chinese Medical Sciences

OBJECTIVE: To investigate the potential chronic liver toxicity of oral administration of ethanol extract of Huangqin(Radix Scutellariae Baicalensis)(SBE) in Wistar ***: SBE was administered to rats by gavage for 26 weeks, at doses of 300, 1250, or 2500 mg·kg-1·d-1 respectively. The rats were euthanized at the end of 13 and 26 weeks daily oral dosing and following 4 weeks of recovery time. The changes of hematology, urinary, blood biochemistry and histomorphology were examined at each time point and focus on liver function and histological ***: When SBE at a dose of up to 2500 mgkg-1 d-1 was fed to male and female rats for 2··6 weeks, the liver tissue showed some inflammatory change that predominated by leukocyte infiltrationbut returned to normal after withdrawal. In addition, high-dose SBE treatment of 26 weeks in rats,glucose, electrolyte and lipid levels also have some changes. In addition, there are no other functional or organic lesions related to SBE ***: Long-term and high-dose SBE may cause liver damage, however, the structural damage of the liver can be restored after the ethanol extract stopping. SBE will be well-tolerated for long-term use as a drug or health food, but in order to ensure drug safety, liver function, and serum glucose, electrolyte and lipid levels should be monitored when using SBE long term.

关键词： Scutellaria baicalensis Toxicity tests chronic Liver Administration oral

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Dragon’s Blood Protect Rat Blood-Brain Barrier Dysfunction Induced by Simulated Microgravity Effect

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Space(Science & Technology) 2023年第1期3卷 487-495页

作者： Ranran Yan yushi zhang YongZhi Li JiaPing Wang Hajira Bibi Yu-Lin Deng YuJuan Li School of Life Science Beijing Institute of TechnologyNo.5 Zhongguancun South StreetHaidian DistrictBeijing 100081China Institute of Chinese Materia Medica No.16 Dongzhimen Neinan StreetDongcheng DistrictBeijing 100081China Astronaut Research and Training Center of China No.109 Youyi RoadHaidian DistrictBeijing 100094China University of Balochistan QuettaPakistan

Dragon’s blood(DB)has shown a protective effect on neurological ***(MG)or simulated MG(SMG)can induce blood-brain barrier(BBB)dysfunction,which is a characteristic feature of neurological *** study’s purpose was to evaluate the effect of DB on SMG-induced BBB dysfunction and explore its signaling *** DB and vitamin C(Vc)were administered orally for tail-suspended rats within 3 *** and Vc solutions were added to human brain microvascular endothelial cells(HCMEC/D3)cells,which were then exposed to SMG for 24 *** protective effect of DB was assessed by hematoxylin and eosin and Nissl staining,ultrastructure observation,and permeability in *** apoptosis and the distribution of tight junction(TJ)and adherens junction(AJ)proteins and filamentous actin(F-actin)were examined in HCMEC/*** oxidative stress and inflammation,and TJ and AJ protein expressions were determined in rat brain and HCMEC/*** focal adhesion kinase(FAK)signaling pathway proteins were *** protected SMG-induced rat BBB disruption by improving neuronal apoptosis,repairing widened intercellular space,and decreasing BBB *** effectively relieved SMG-induced HCMEC/D3 damage by inhibiting cell apoptosis and restoring F-actin spindle *** doses of DB upregulated TJ and AJ protein expressions and decreased oxidative stress and proinflammatory cytokine levels in rat brain and HCMEC/*** enhanced the expressions of FAK signal transduction proteins and F-actin/globular actin(G-actin)ratio in rat brain and HCMEC/D3,suggesting that DB promotes actin cytoskeleton polymerization,benefits the endothelial cell-cell and cell-extracellular matrix adhesion,and,in consequence,contributes to BBB integrity.

关键词： Blood protective doses

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