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Fluorescence emission difference microscopy based on polarization modulation

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Journal of Innovative Optical Health Sciences 2022年第5期15卷 83-94页

作者： Wanjie Dong yuran huang Zhimin Zhang Liang Xu Cuifang Kuang Xiang Hao Xu Liu State Key Laboratory of Modern Optical Instrumentation College of Optical Science and Engineering Zhejiang University Hangzhou 310027P.R.China Research Center for Intelligent Chips and Devices Zhejiang Lab Hangzhou 311121P.R.China Ningbo Research Institute Zhejiang University NingboZhejiang 315100P.R.China Collaborative Innovation Center of Extreme Optics Shanxi University Taiyuan 030006P.R.China State Key Laboratory of Precision Measurement Technology and Instruments Department of Precision Instruments Tsinghua UniversityBeijing 100084P.R.China

In this paper,we propose a new fluorescence emission difference microscopy(FED)technique based on polarization *** electro-optical modulator(EOM)is used to switch the excitation beam between the horizontal and vertical polarization states at a high frequency,which leads to solid-and donut-shaped beams after spatial light *** on the fluorescent nanoparticles demonstrates that the proposed method can achieve~λ=4 spatial *** the proposed system,the dynamic imaging of subcellular structures in living cells over time is achieved.

关键词： Super-resolution fluorescence emission difference microscopy electro-optical modulator polarization modulation

Multiplexed stimulated emission depletion nanoscopy(mSTED)for 5-color live-cell long-term imaging of organelle interactome

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Opto-Electronic Advances 2024年第7期7卷 17-26页

作者： yuran huang Zhimin Zhang Wenli Tao Yunfei Wei Liang Xu Wenwen Gong Jiaqiang Zhou Liangcai Cao Yong Liu Yubing Han Cuifang Kuang Xu Liu State Key Laboratory of Extreme Photonics and Instrumentation College of Optical Science and EngineeringZhejiang UniversityHangzhou 310027China Research Center for Intelligent Chips and Devices Zhejiang LabHangzhou 311121China Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan 430074China Department of Endocrinology and Metabolism Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Department of Precision Instruments Tsinghua UniversityBeijing 100084China College of electronics and information engineering Shanghai University of Electrical PowerShanghai 200090China ZJU-Hangzhou Global Scientific and Technological Innovation Center Hangzhou 311200China

Stimulated emission depletion microscopy(STED)holds great potential in biological science applications,especially in studying nanoscale subcellular ***,multi-color STED imaging in live-cell remains challenging due to the limited excitation wavelengths and large amount of laser ***,we develop a multiplexed live-cell STED method to observe more structures simultaneously with limited photo-bleaching and *** separating live-cell fluorescent probes with similar spectral properties using phasor analysis,our method enables five-color live-cell STED imaging and reveals long-term interactions between different subcellular *** results here provide an avenue for understanding the complex and delicate interactome of subcellular structures in live-cell.

关键词： optical nanoscopy phasor analysis multicolor live cell imaging

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基于反卷积的手机屏下成像图像恢复

光电工程 2021年第6期48卷 10-20页

作者：王晓娜黄宇然匡翠方李海峰刘旭浙江大学光电工程学院现代光学仪器国家重点实验室浙江杭州310027 浙江大学宁波研究院浙江宁波315100

随着全面屏手机的发展,手机屏下成像的研究应运而生。但由于手机屏幕存在电路走线和其他不透明部分,光通过屏幕会产生衍射,降低成像结果的图像质量,本文从图像复原的角度,对屏下相机拍摄的图像进行恢复。通过测量得到手机成像系统的点... 详细信息

随着全面屏手机的发展,手机屏下成像的研究应运而生。但由于手机屏幕存在电路走线和其他不透明部分,光通过屏幕会产生衍射,降低成像结果的图像质量,本文从图像复原的角度,对屏下相机拍摄的图像进行恢复。通过测量得到手机成像系统的点扩散函数(PSF),利用测得的PSF,对图像进行反卷积处理。本文改进了传统的反卷积方法,对原始图像进行颜色空间转换,然后对不同的通道分别处理。相较于传统反卷积方法,改进后的反卷积方法得到的处理结果在结构相似度(SSIM)、峰值信噪比(PSNR)等指标上都有提高,运行时间更短。在分通道反卷积之后,使用非局部平均算法进行去噪处理,进一步提高了屏下图像的质量。

关键词：反卷积去噪屏下成像图像复原

不同鲜味物质对干腌马鲛鱼鲜味的贡献与比较分析

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食品科学 2021年第16期42卷 138-144页

作者：黄煜燃汪薇赵文红杨娟刘巧瑜白卫东仲恺农业工程学院轻工食品学院广东广州510225

以8种市售干腌马鲛鱼为研究对象,通过比较不同产品中鲜味物质成分和含量差异,并结合其滋味强度值和味精当量的分析计算,以确定对干腌马鲛鱼鲜味贡献最大的物质。结果表明:干腌马鲛鱼具有突出的咸、鲜味和浓厚味感,而其鲜味主要是由谷氨... 详细信息

以8种市售干腌马鲛鱼为研究对象,通过比较不同产品中鲜味物质成分和含量差异,并结合其滋味强度值和味精当量的分析计算,以确定对干腌马鲛鱼鲜味贡献最大的物质。结果表明:干腌马鲛鱼具有突出的咸、鲜味和浓厚味感,而其鲜味主要是由谷氨酸、鲜味肽等鲜味物质和乳酸等增鲜物质赋予。在其鲜味物质中,谷氨酸的滋味强度值大于1(除3、5、7号样品),显示出它对干腌马鲛鱼鲜味的突出贡献,超滤组分的感官分析和肽分子质量分布测定结果表明,分子质量小于1000 Da的小分子肽对样品的鲜味具有重要贡献。此外,长时间的风干可能会促进乳酸和鲜味肽的生成,但随着水分的流失,其苦味和咸味也增高;而辅料蔗糖和白酒的添加将抑制乳酸的产生,且蔗糖能在一定程度上促进谷氨酸的生成。值得强调的是,8种样品的核苷酸均已降解为肌苷(inosine,HxR)和次黄嘌呤(hypoxanthine,Hx)等苦味物质,且长时间的风干可能会促进HxR和Hx的积累。

关键词：干腌马鲛鱼鲜味物质滋味强度值味精当量

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共路并行荧光辐射差分超分辨显微成像

中国激光 2021年第16期48卷 125-131页

作者：张智敏黄宇然刘少聪匡翠方曹良才刘勇韩于冰郝翔刘旭浙江大学光电科学与工程学院现代光学仪器国家重点实验室浙江杭州310027 浙江大学宁波研究院浙江宁波315100 山西大学极端光学协同创新中心山西太原030006 清华大学精密测试技术及仪器国家重点实验室北京100084 上海电力学院上海200090

本文提出了一种共路并行荧光辐射差分超分辨显微成像方法,利用单个空间光调制器(SLM)同时产生两个相位灰度图,对入射激光的水平偏振分量和垂直偏振分量同时进行调制,使得最终的会聚光场由错开的高斯实心光斑和空心光斑组成。在样品面上... 详细信息

本文提出了一种共路并行荧光辐射差分超分辨显微成像方法,利用单个空间光调制器(SLM)同时产生两个相位灰度图,对入射激光的水平偏振分量和垂直偏振分量同时进行调制,使得最终的会聚光场由错开的高斯实心光斑和空心光斑组成。在样品面上,利用错开的实心光斑和空心光斑同时对样品进行扫描,与此同时在探测端利用两个探测器同时收集错开光斑所激发的荧光信号,采用荧光辐射差分(FED)方法对采集的图像进行处理,就可以实现对样品的快速超分辨成像。与传统的并行荧光辐射差分超分辨显微术相比,本方法在保留了将成像速度提高一倍的优势的同时克服了非共路并行系统中不同器件引入的噪声、漂移对图像质量的影响,并简化了光路。实验结果表明所提共路并行荧光辐射差分超分辨显微成像方法具有良好的超衍射极限成像能力。

关键词：显微荧光显微镜快速超分辨成像荧光辐射差分技术共路并行探测

大数据背景下北京市大型居住区通勤绿道选线研究

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风景园林 2018年第8期25卷 30-35页

作者：梁军辉杜洋赛金波黄昱然北京清华同衡规划设计研究院北京大学政府管理学院

近年来,随着城市交通拥堵和环境污染等问题的日益凸显,自行车作为通勤出行方式的地位逐渐上升,通勤者对建立不受机动车干扰的、连续的、具备通勤功能的城市绿色道路的需求也在不断增加。绿色道路的线路建设不仅要保障空间可实施性和交... 详细信息

近年来,随着城市交通拥堵和环境污染等问题的日益凸显,自行车作为通勤出行方式的地位逐渐上升,通勤者对建立不受机动车干扰的、连续的、具备通勤功能的城市绿色道路的需求也在不断增加。绿色道路的线路建设不仅要保障空间可实施性和交通环境质量,还要与居民的通勤出行需求紧密结合,实现绿道使用的综合效益最大化。基于此,研究以北京市回龙观地区为对象,采用与居民出行行为有关的数据,识别郊区居民中短程通勤特征,划定主要通勤范围,探究基于居民真实出行的多源大数据的选线方法,通过基于通勤出行需求强度与吸引强度的绿道节点甄选与路段适宜性的评价分析。最后,通过GIS网络分析功能进行最优线路选择,并结合城市公园、绿地、河流等资源的分布情况进行修正,形成一条满足居民通勤需求的、完整互联的、安全的且具备较强实施性的绿色通道。最终达到降低居民通勤时耗、提升区域可达性、完善城市慢行系统网络的目的。

关键词：风景园林绿道通勤绿道选线大数据北京市

Predicting Hurricane Evacuation Decisions with Interpretable Machine Learning Methods

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International Journal of Disaster Risk Science 2024年第1期15卷 134-148页

作者： yuran Sun Shih‑Kai huang Xilei Zhao Department of Civil and Coastal Engineering University of FloridaGainesvilleFL 32611USA Department of Emergency Management and Public Administration Jacksonville State UniversityJacksonvilleAL 36265USA

Facing the escalating effects of climate change,it is critical to improve the prediction and understanding of the hurricane evacuation decisions made by households in order to enhance emergency *** studies in this area often have relied on psychology-driven linear models,which frequently exhibited limitations in *** present study proposed a novel interpretable machine learning approach to predict household-level evacuation decisions by leveraging easily accessible demographic and resource-related predictors,compared to existing models that mainly rely on psychological *** enhanced logistic regression model(that is,an interpretable machine learning approach) was developed for accurate predictions by automatically accounting for nonlinearities and interactions(that is,univariate and bivariate threshold effects).Specifically,nonlinearity and interaction detection were enabled by low-depth decision trees,which offer transparent model structure and robustness.A survey dataset collected in the aftermath of Hurricanes Katrina and Rita,two of the most intense tropical storms of the last two decades,was employed to test the new *** findings show that,when predicting the households’ evacuation decisions,the enhanced logistic regression model outperformed previous linear models in terms of both model fit and predictive *** outcome suggests that our proposed methodology could provide a new tool and framework for emergency management authorities to improve the prediction of evacuation traffic demands in a timely and accurate manner.

关键词： Artifcial Intelligence(AI) Decision-making modeling Hurricane evacuation Interpretable machine learning Nonlinearity and interaction detection

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速冻面制品冷链物流的存在问题与分析

现代食品 2020年第3期5卷 6-10页

作者：包沛贤黄煜燃潘振辉白卫东刘晓艳广州酒家集团利口福食品有限公司广东番禺511442 仲恺农业工程学院广东广州510225

近年来,人们对速冻面制品的品质要求日益提高,因此刺激了冷链物流行业的发展。但我国的冷链物流仍然存在许多问题,在发展过程中受到了严重的障碍。本文就冷链物流的发展和应用现状入手,分析了当前冷链物流存在的问题,最后提出冷链物流... 详细信息

近年来,人们对速冻面制品的品质要求日益提高,因此刺激了冷链物流行业的发展。但我国的冷链物流仍然存在许多问题,在发展过程中受到了严重的障碍。本文就冷链物流的发展和应用现状入手,分析了当前冷链物流存在的问题,最后提出冷链物流健康发展的对策和启示。

关键词：冷链物流速冻面制品冷链物流建设对策分析

Coupling mechanism of activated carbon mixed with dust for flue gas desulfurization and denitrification

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Journal of Environmental Sciences 2020年第12期32卷 205-214页

作者： Junxiang Guo yuran Li Jin Xiong Tingyu Zhu Beijing Engineering Research Center of Process Pollution Control National Engineering Laboratory for Hydrometallurgical Cleaner Production TechnologyInstitute of Process EngineeringChinese Academy of SciencesBeijing 100190China Center for Excellence in Regional Atmospheric Environment Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China

To clarify the effect of coking dust, sintering dust and fly ash on the activity of activated carbon for various industrial flue gas desulfurization and denitrification, the coupling mechanism of the mixed activated carbon and dust was investigated to provide theoretical reference for the stable operation. The results show that coking dust had 34% desulfurization efficiency and 10% denitrification efficiency;correspondingly, sintering dust and fly ash had no obvious desulfurization and denitrification activities. For the mixture of activated carbon and dust, the coking dust reduced the desulfurization and denitrification efficiencies by blocking the pores of activated carbon, and its inhibiting effect on activated carbon was larger than its own desulfurization and denitrification activity. The sintering dust also reduced the desulfurization efficiency on the activated carbon while enhancing the denitrification efficiency. Fly ash blocked the pores of activated carbon and reduced its reaction activity. The reaction activity of coking dust mainly came from the surface functional groups, similar to that of activated carbon. The reaction activity of sintering dust mainly came from the oxidative property of Fe_2O _3, which oxidized NO to NO_2 and promoted the fast selectively catalytic reduction(SCR) of NO to form N_2. Sintering dust was activated by the joint action of activated carbon, and both had a coupling function. Sintering dust enhanced the adsorption and oxidation of NO, and activated carbon further promoted the reduction of NO_x by NH_3;thus, the denitrification efficiency increased by 5%-7% on the activated carbon.

关键词： Activated carbon Coking dust Sintering dust Fly ash Coupling effect