Fluorescence emission difference microscopy based on polarization modulation
作者机构：State Key Laboratory of Modern Optical Instrumentation College of Optical Science and Engineering Zhejiang UniversityHangzhou 310027P.R.China Research Center for Intelligent Chips and Devices Zhejiang LabHangzhou 311121P.R.China Ningbo Research InstituteZhejiang University NingboZhejiang 315100P.R.China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006P.R.China State Key Laboratory of Precision Measurement Technology and InstrumentsDepartment of Precision Instruments Tsinghua UniversityBeijing 100084P.R.China
出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)
年 卷 期：2022年第15卷第5期
页 面：83-94页
核心收录：
学科分类：0808[工学-电气工程] 081704[工学-应用化学] 0809[工学-电子科学与技术（可授工学、理学学位）] 07[理学] 08[工学] 070302[理学-分析化学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0836[工学-生物工程] 0803[工学-光学工程] 0702[理学-物理学]
基 金：supported in part by the National Natural Science Foundation of China(61827825,62125504,and 61735017) Major Program of the Natural Science Foundation of Zhejiang Province(LD21F050002) Key Research and Development Program of Zhejiang Province(2020C01116) Zhejiang Lab(2020MC0AE01) China Postdoctoral Science Foundation(BX2021272)
主 题：Super-resolution fluorescence emission difference microscopy electro-optical modulator polarization modulation
摘 要：In this paper,we propose a new fluorescence emission difference microscopy(FED)technique based on polarization *** electro-optical modulator(EOM)is used to switch the excitation beam between the horizontal and vertical polarization states at a high frequency,which leads to solid-and donut-shaped beams after spatial light *** on the fluorescent nanoparticles demonstrates that the proposed method can achieve~λ=4 spatial *** the proposed system,the dynamic imaging of subcellular structures in living cells over time is achieved.
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