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Multilocus DNA metabarcoding diet analyses of small cetaceans:a case study on highly vulnerable humpback dolphins and finless porpoises from the Pearl River Estuary,China

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Integrative Zoology 2023年第1期18卷 183-198页

作者： Xiyang ZHANG Dingyu LUO Ri-Qing YU yuping wu School of Marine Sciences Zhuhai Key Laboratory of Marine Bioresources and EnvironmentGuangdong Provincial Key Laboratory of Marine Resources and Coastal EngineeringPearl River Estuary Marine Ecosystem Research StationMinistry of EducationSun Yat-sen UniversitySouthern Marine Science and Engineering Guangdong LaboratoryZhuhaiChina Department of Biology Center for EnvironmentBiodiversity and ConservationThe University of Texas at TylerTylerTexasUSA

Accurate diet identiﬁcation of top predators is crucial to fully understand their ecological *** to terrestrial animals,gathering dietary information from cetaceans is notoriously difﬁ***,we applied a multilocus metabarcoding approach to investigate the diet of vulnerable Indo-Paciﬁc humpback dolphins and Indo-Paciﬁcﬁnless porpoises from the Pearl River Estuary(PRE),*** analyses identiﬁed 21 preyﬁsh species from the 42 humpback dolphin stomachs,as well as 10 species ofﬁsh and 1 species of cephalopod from the 13ﬁnless porpoise *** of the taxa were assigned to the species level,highlighting that the multimarker approach could facilitate species identiﬁ*** of the prey species were small-and medium-sizedﬁshes that primarily fed on *** calculated similarity index revealed a moderated dietary overlap between the 2 cetaceans,presumably due to the feeding of the 2 predators in association withﬁshing vessels in the PRE.A more diverse diet was observed in humpback dolphins in the closedﬁshing season compared to theﬁshing season,implying the inﬂuence on the dolphin diet due to the availability of commercialﬁshery ***,according to the results of species rarefaction curves,ourﬁndings on the feeding habits of the 2 cetaceans are still limited by insufﬁcient sample size and therefore should be interpreted with *** study represents aﬁrst attempt to apply the multilocus DNA metabarcoding technique in the diet analysis of small cetaceans,although more efforts are needed to improve this type of analysis.

关键词： diet species analyses DNA metabarcoding Indo-Paciﬁcﬁnless porpoise Indo-Paciﬁc humpback dolphin Pearl River Estuary

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姜蛋白脱除刺梨果汁中单宁的工艺研究

广西植物 2024年第6期44卷 1052-1059页

作者：刘治年吴豫平王欣颖田弋夫林剑余德顺贵州大学化学与化工学院贵阳550025 中国科学院地球化学研究所环境地球化学国家重点实验室超临界流体技术研究中心贵阳550081 贵州初好农业科技开发有限公司贵州六盘水553000

为有效脱除刺梨果汁(Rosa roxburghii juice)中的单宁,降低其涩味改善口感,该研究以刺梨果汁为对象,采用化学沉淀法,以姜蛋白为单宁脱除剂,并以单宁脱除率和维生素C(VC)保留率为考察指标,采用单因素实验和正交试验优化姜蛋白脱除单宁工... 详细信息

为有效脱除刺梨果汁(Rosa roxburghii juice)中的单宁,降低其涩味改善口感,该研究以刺梨果汁为对象,采用化学沉淀法,以姜蛋白为单宁脱除剂,并以单宁脱除率和维生素C(VC)保留率为考察指标,采用单因素实验和正交试验优化姜蛋白脱除单宁工艺确定最优工艺。结果表明:(1)姜蛋白脱除刺梨果汁单宁的最优工艺条件为液固比30∶1.2(mL∶g),刺梨果汁pH 3.0,搅拌温度5℃,搅拌时间30 min。(2)由正交试验分析可知,各因素对刺梨果汁脱除单宁的影响程度依次为液固比>搅拌温度>刺梨果汁pH>搅拌时间。(3)在最优工艺条件下,单宁脱除率为(47.451±0.608)%,VC保留率为(75.904±1.244)%。(4)在最优工艺条件下,果汁透光率从(8.44±0.662)%提高到(92.47±0.397)%,涩味明显改善,同时丰富了刺梨果汁风味。该研究结果为解决刺梨果汁深加工行业面临的共性关键技术问题提供了一个新思路和新工艺技术路线基础,也为拓展生姜资源的综合利用奠定了一定技术基础。

关键词：刺梨果汁姜蛋白单宁化学沉淀法维生素C(VC)

Synthesis of Lithium Garnet Oxides of the Compositions Series Li7-xLa3Zr2-xTaxO12

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Journal of wuhan University of Technology(Materials Science) 2017年第6期32卷 1261-1264页

作者：王治安 MO Jun wu yuping 叶红齐 wu Xiongwei Department oJChemistry and Chemical Engineering Central South University Changsha 410083 China College of Science National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanica College of Agronomy HunanAgricultural University Changsha 410128 China New Energy and Materials Laboratory (NEML) Department of Chemistry & ShanghaiKey Laboratory of Molecular Catalysis and Innovative Materials Fudan University Shanghai 200433 China College of Energy Nanjing University of Technology Nanjing 211800 China

In order to obtain a safe,reliable,long-lived battery system without use of flammable,volatile,and relatively unstable organic liquid-based electrolytes,lithium garnet oxides with formulas Li7-xLa3Zr2-xTaxO12（x=0.2-1）were synthesized by the solid state reaction *** cubic phases were observed in the composition x range between 0.2 and *** lattice parameters decreased with the addition of Ta due to the smaller ionic radius of Ta^5＋compared with that of Zr^4＋,following the Vegard＇s *** total conductivity of the x=0.3 composition is 6.03×10^-5 S·cm^-1at room temperature with an activation energy of 0.30 *** lithium garnet oxides exhibit lithium ionic transport that is relevant to lithium battery application.

关键词： solid electrolytes cubic garnet Ta substitution ionic conductivity

Chromatographic Resolution of a-Amino Acids by （R）-（3,3＇-Halogen Substituted-l,l＇-binaphthyl）-20-crown-6 Stationary Phase in HPLC

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Chinese Journal of Chemistry 2017年第7期35卷 1037-1042页

作者： Peng wu yuping wu JunhuiZhang Zhenyu Lu Mei Zhang Xuexian Chen Liming Yuan Department of Chemistry Yunnan Normal University Kunming Yunnan 650500 China Department of Traditional Chinese Medicine Yunnan University of Traditional Chinese Medicine Kunming Yunnan 650500 China

Three new chiral stationary phases （CSPs） for high-performance liquid chromatography were prepared from R-（3,3＇-halogen substituted-1,1＇-binaphthyl）-20-crown-6 （halogen=C1, Br and I ）. The experimental results showed that R-（3,3＇-dibromo-l,l＇-binaphthyl）-20-crown-6 （CSP-1） possesses more prominent enantioselectivity than the two other halogen-substituted crown ether derivatives. All twenty-one a-amino acids have different degrees of sep- aration on R-（3,3＇-dibromo-l,l＇-binaphthyl）-20-crown-6-based CSP-1 at room temperature. The enantioselectivity of CSP-1 is also better than those of some commercial R-（1, 1＇-binaphthyl）-20-crown-6 derivatives. Both the separa- tion factors （a） and the resolution （Rs） are better than those of commercial crown ether-based CSPs [CROWNPAK CR（＋） from Daicel] under the same conditions for asparagine, threonine, proline, arginine, serine, histidine and va- line, which cannot be separated by commercial CR（＋）. This study proves the commercial usefulness of the R-（3,3＇-dibromo- 1, 1＇-binaphthyl）-20-crown-6 chiral stationary phase.

关键词： α-amino acid chiral crown ethers stationary phase high-performance liquid chromatography

Tailoring the Porous Structure of Mono-dispersed Hierarchically Nitrogen-doped Carbon Spheres for Highly Efficient Oxygen Reduction Reaction

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Energy & Environmental Materials 2021年第1期4卷 81-87页

作者： Chengyong Shu Zhuofan Gan Yuyang Hou Ting Zhu Jijun Ma Wei Tang yuping wu School of Chemical Engineering and Technology Xi'an Jiaotong UniversityXi'an 710049China CSIRO Mineral Resources Clayton Vic 3168Australia School of Materials Science and Engineering Central South UniversityChangsha 410083China State Key Laboratory of Space Power-Sources Technology Shanghai Institute of Space Power-SourcesShanghai 200245China State Key Laboratory of Materials-Oriented Chemical Engineering School of Energy Science and Engineering and Institute of Advanced MaterialsNanjing Tech UniversityNanjing 211800China

The search for a low-cost metal-free cathode material with excellent mass transfer structure and catalytic activity in oxygen reduction reaction(ORR)is one of the most challenging issues in fuel *** this work,nitrogen-rich mphenylenediamine is introduced into the synthesis of porous carbon spheres to tune the pore structure and nitrogen-doped active *** a result,more pyridinic N and pyrrolic N functional species were observed at the interior and surface of the carbon *** introduction of m-phenylenediamine also regulated the nucleating of precursors,an urchin-like mesoporous surface structure ensures point contact and less agglomeration between each particle was *** optimized proportion of micropores/mesopores and improved nitrogen-contained functional species,the ORR activity can be remarkably *** half-wave potential of this catalyst could achieve to 0.81 V(versus RHE)which is only 42 m V lower than commercial Pt/C ***,the optimized cathode catalyst achieved a 69 m W cm-2 maximum power density when operated in direct methanol fuel cells at room temperature.

关键词： fuel cells metal-free catalysts nitrogen-doped porous carbon oxygen reduction reaction

A preliminary investigation on genetic diversity of Sousa chinensis in the Pearl River Estuary and Xiamen of Chinese waters

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Journal of Genetics and Genomics 2008年第8期35卷 491-497页

作者： Hongshan Chen Kan Zhai Jialin Chen Yulong Chen Haijun Wen Sufang Chen yuping wu State Key Laboratory of Biocontrol Department of Biochemistry School of Life Sciences Sun Yat-Sen University Guangzhou 510275 China National Nature Preserve Administration Bureau on Sousa chinensis of Pearl River Estuary Zhuhai 519000 China The First Affiliated Hospital of Sun Yat-Sen University Guangzhou 510000 China

In this study, the mitochondrial DNA （mtDNA） control region and the mitochondrial cytochrome b gene of stranded Indo-Pacific humpback dolphin （Sousa chinensis） samples from the Pearl River Estuary and Xiamen waters were sequenced and analyzed. The result of mtDNA control region revealed 34 variable sites and four unique haplotypes （named as A, B, C and D） identified among the total samples from these two water areas, and the most common haplotype （A） was shared by 75% of the dolphins sampled from the two water areas. The haplotypic diversity （h） was 0.455 and the nucleodde diversity （π） was 0.0088. The phylogenetic analysis showed that the haplotype A, C, and D were closely related, but the haplotype B （unique for XM01 from Xiamen） was far from the other three. By scanning cytochrome b fragments, two haplotypes （A and B） were identified in these two water areas, and the most common haplotype （A） was shared by 91.67% individuals, while XM01 from Xiamen as the only exception. The data suggest that there is a possibility of gene exchange between the two populations in the Pearl River Estuary and Xiamen waters, and there possibly exists a unique maternal lineage in Xiamen waters.

关键词： Sousa chinensis mitochondrial DNA control region cytochrome b genetic diversity

Advances in Sn-Based Catalysts for Electrochemical CO_(2) Reduction

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Nano-Micro Letters 2019年第4期11卷 114-132页

作者： Shulin Zhao Sheng Li Tao Guo Shuaishuai Zhang Jing Wang yuping wu Yuhui Chen State Key Laboratory of Materials-oriented Chemical Engineering School of Energy Science and EngineeringNanjing Tech UniversityNanjing 211816JiangsuPeople’s Republic of China

The increasing concentration of CO2 in the atmosphere has led to the greenhouse effect,which greatly affects the climate and the ecological balance of ***,converting CO2 into renewable fuels via clean and economical chemical processes has become a great concern for *** CO2 conversion is a prospective path toward carbon *** the different electrocatalysts,Sn-based electrocatalysts have been demonstrated as promising catalysts for CO2 electroreduction,producing formate and CO,which are important industrial *** this review,various Sn-based electrocatalysts are comprehensively summarized in terms of synthesis,catalytic performance,and reaction mechanisms for CO2 ***,we concisely discuss the current challenges and opportunities of Sn-based electrocatalysts.

关键词： Greenhouse effect CO_(2) electrochemical reduction Sn-based electrocatalysts

Enhanced Capacitive Desalination Performance of Porous Carbon Spheres@MnO2 Composite

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Chinese Journal of Chemistry 2017年第1期35卷 55-60页

作者： Bingwei Chen Yanfang Wang Feng Yu Yusong Zhu Lixin Zhang yuping wu New Energy and Material Laboratory (NEML) Department of Chemistry & Shanghai Key Laboratory of Molecular Catalysis and lnnovative Material Fudan University Shanghai 200433 China College of Energy and Institute for Electrochemical Energy Storage Nanjing Tech University Nanjing Jiangsu 211816 China

A porous carbon spheres@MnO2 composite is prepared and used as the electrosorption electrode. The MnO2 nanoparticles are evenly distributed on the porous carbon spheres. The as-prepared composite possesses high specific surface area of 258.6 m2·g -1. Its electrochemical evaluations confirm that the composite has high specific capacitance of 175 Fog-1 at a current density of 1 A·g -1 in 0.5 mol·L-1 NaC1 aqueous solution, and shows good conductivity and excellent cycling performance. Electrosorption capacity for the porous carbon spheres@MnO2 composite is 6.28 mg·g-1. Moreover, the composite electrode exhibits excellent recyclability with an efficient regeneration process.

关键词： carbon nanostructure composite electrochemical properties desalination performance

Advances of Aluminum Based Energy Storage Systems

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Chinese Journal of Chemistry 2017年第1期35卷 13-20页

作者： Lijun Fu Na Li Yu Liu Weigang Wang Yusong Zhu yuping wu College of Energy Science and Engineering and Institute of Advanced Materials Nanjing Tech University No. 30 Puzhu Road (S) Nanjing Jiangsu 211800 China

Rechargeable aluminum based batteries and supercapacitors have been regarded as promising sustainable ener- gy storage candidates, because aluminum metal is the most abundant metal element in the earth crust, and it delivers very high volumetric capacity and acceptable gravimetric capacity. This mini review presents the brief development of Al-based primary batteries, and introduces the latest advances of rechargeable Al-based energy storage systems with both nonaqueous and aqueous electrolytes.

关键词： aluminum battery supercapacitor aqueous and nonaqueous electrolytes