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Preparation and properties of a silver particle-coated and 1-dodecanethiol-modified superhydrophobic melamine sponge for oil/water separation

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Frontiers of Chemical Science and Engineering 2022年第8期16卷 1237-1246页

作者： Xiaofei Sun Shijie Feng Zhe Zhang Pengyu Zhang Jiefeng Zhao yunling gao Junxian Yun State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology College of Chemical EngineeringZhejiang University of TechnologyHangzhou 310032China

A Ag particle-coated and 1-dodecanethiolmodified melamine sponge(Ag-DDT-MS)was prepared through surface roughness by coating silver particles and subsequent grafting of a hydrophobic long hydrocarbon *** and 3D porous Ag-DDT-MS was characterized by Fourier transform infrared spectroscopy,scanning electron microscope,energy-dispersive X-ray spectroscopy,and X-ray *** water contact angle of Ag-DDT-MS reached 159.2°.Ag-DDT-MS exhibited excellent absorption capacity for high viscous oils and organic solvents,ranging from 42.8 to 105.2 g∙g−*** absorbed oils can be easily collected by the mechanical pressing process,and the oil recovery rate was satisfactory,more than 90%after 20 ***-DDT-MS material also demonstrated good stability and excellent compressionrecovery ability,keeping 88.6%of the initial height after ten compression-release cycles.

关键词： melamine sponge oil-absorbing material,hydrophobic oil-water separation oil absorption capacity

Imaging and Spectral Characteristics of Amyloid Plaque Autofluorescence in Brain Slices from the APP/PS1 Mouse Model of Alzheimer’s Disease

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Neuroscience Bulletin 2019年第6期35卷 1126-1137页

作者： yunling gao Qing Liu Lingling Xu Ning Zheng Xiaoming He Fuqiang Xu Research Section Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceXiangyang 441021China Center for Brain Science State Key Laboratory of Magnetic Resonance and Atomic Molecular PhysicsNational Center for Magnetic Resonance in WuhanKey Laboratory of Magnetic Resonance in Biological SystemsWuhan Institute of Physics and MathematicsChinese Academy of SciencesWuhan 430071China National Center for Magnetic Resonance in Wuhan State Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsWuhan Institute of Physics and MathematicsChinese Academy of SciencesWuhan 430071China Neurology Department Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceXiangyang 441021China Wuhan National Laboratory for Optoelectronics Wuhan 430074China

Amyloid deposits are one of the hallmark pathological lesions of Alzheimer's disease(AD). They can be visualized by thioflavin-S, silver impregnation,Congo red staining, and immunohistochemical ***, that amyloid deposits generate blue autofluorescence(auto-F) has been ignored. Here, we report that visible light-induced auto-F of senile plaques(SPs) was detected and validated with conventional methods. Brain slices from APP/PS1(amyloid precursor protein/presenilin1) transgenic mice were mounted on slides, rinsed,coverslipped and observed for details of the imaging and spectral characteristics of the auto-F of SPs. Then the slices were treated with the above classic methods for comparative validation. We found that the SP auto-F was greatest under blue-violet excitation with a specific emission spectrum, and was much easier, more sensitive, and reliable than the classic methods. Because it does not damage slices, observation of auto-F can be combined with all post-staining techniques in slices and for brain-wide imaging in AD.

关键词： Alzheimer's disease Amyloid deposits Senile plaques Autofluorescence Spectral imaging Glial activation

反应型HClO/ClO^(-)荧光探针的研究进展

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化工进展 2021年第6期40卷 3346-3362页

作者：安宁高云玲浙江工业大学化学工程学院浙江杭州310014

次氯酸/次氯酸根是活性氧化物中重要的信号分子,在生物医药和环境安全等方面发挥着重要作用。反应型荧光探针基于出色的灵敏度、实时成像和生物兼容性好等特点,广泛应用于HClO/ClO^(-)的检测。本文评述了近5年反应型HClO/ClO^(-)荧光探... 详细信息

次氯酸/次氯酸根是活性氧化物中重要的信号分子,在生物医药和环境安全等方面发挥着重要作用。反应型荧光探针基于出色的灵敏度、实时成像和生物兼容性好等特点,广泛应用于HClO/ClO^(-)的检测。本文评述了近5年反应型HClO/ClO^(-)荧光探针的研究进展,包括反应型HClO/ClO^(-)荧光探针的设计策略和识别机理,并从HClO/ClO^(-)引发的反应机理角度(碳碳双键的反应、硫族化合物的反应、醛肟基团的反应、腙/席夫碱的反应、酰肼/磺酰肼的反应、N,N-二甲基硫代氨基甲酸酯的反应和苯硼酸/硼酸酯的反应)概述了反应型HClO/ClO^(-)荧光探针的特点和实际应用,指出反应型HClO/ClO^(-)荧光探针的发展方向是合成性能优异的识别基团,构建选择性好、水溶性好、低荧光背景、光化学性能稳定和生物毒性低的反应型近红外HClO/ClO^(-)荧光探针,实现对生物体内外HClO/ClO^(-)的可视化检测及机理探索。

关键词：次氯酸根荧光探针检测应用可视化

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反应型荧光探针在检测金属离子中的研究进展

化工进展 2016年第10期35卷 3288-3294页

作者：张聪高云玲浙江工业大学化学工程学院浙江杭州310032

金属离子广泛存在于自然界中,与环境科学、生命科学、医学等领域有着密切的联系。一些金属离子如Hg2+、Fe3+、Cu2+、Zn2+、Al3+等,在生物体的生理和病理中扮演着重要角色,摄入过量或不足都会导致生理功能的紊乱,引发各种疾病。近几年来... 详细信息

金属离子广泛存在于自然界中,与环境科学、生命科学、医学等领域有着密切的联系。一些金属离子如Hg2+、Fe3+、Cu2+、Zn2+、Al3+等,在生物体的生理和病理中扮演着重要角色,摄入过量或不足都会导致生理功能的紊乱,引发各种疾病。近几年来,反应型荧光探针因其高选择性和高灵敏性的特点得到了快速的发展。本文主要综述了近五年来反应型荧光探针在检测金属离子中的研究进展,主要介绍了各类探针分子的设计合成、传感机理、检测结果以及其在生物检测中的应用。指出反应型荧光探针的研究发展还处在初级阶段,该领域将会朝着灵敏度更高、反应时间更少、应用范围更广的方向发展,此外,反应型荧光探针在生物检测以及实际应用方面也有望得到进一步的应用发展。

关键词：化学反应探针选择性金属离子

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动物疫病监测样本采集的注意问题

中国家禽 2009年第2期31卷 47-48页

作者：高云玲北京市兽医实验诊断所北京100101

动物疫病监测在动物疫病防控中作用巨大，实验室检测是目前动物疾病监测的重要内容，特别是传染性疫病更离不开实验室检测，实验结果的准确性、有效性除实验方法、检测试剂、实验操作等因素影响外，从一开始就受到样本的采集、保存、运... 详细信息

动物疫病监测在动物疫病防控中作用巨大，实验室检测是目前动物疾病监测的重要内容，特别是传染性疫病更离不开实验室检测，实验结果的准确性、有效性除实验方法、检测试剂、实验操作等因素影响外，从一开始就受到样本的采集、保存、运输等因素的影响。特别是在开展动物疫病的监测工作，监测结果是否具有代表性，是否能够真实反映出疫病的真实情况都与监测样本的采集有重要关系。

关键词：样本采集疫病监测动物疫病注意问题实验室检测疾病监测检测试剂因素影响

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基于苯并噻唑的次氯酸荧光探针的合成及应用

分析科学学报 2024年第2期40卷 159-164页

作者：王丹高云玲浙江工业大学化学工程学院浙江杭州310014

该文以苯并噻唑衍生物和二氨基马来腈为原料,经醛胺缩合制备了一种高选择性的次氯酸(HClO)荧光探针(HADM)。探针HADM在活性氧和活性氮物种的干扰下能对HClO进行快速特异性识别(反应时间<3s)且具有良好的pH稳定性,同时可以观察到明显的... 详细信息

该文以苯并噻唑衍生物和二氨基马来腈为原料,经醛胺缩合制备了一种高选择性的次氯酸(HClO)荧光探针(HADM)。探针HADM在活性氧和活性氮物种的干扰下能对HClO进行快速特异性识别(反应时间<3s)且具有良好的pH稳定性,同时可以观察到明显的绿色荧光。在优化条件下,探针的荧光强度与HClO的浓度呈良好的线性关系,相关系数R~2=0.9969,检测限为2.64×10^(-6)mol/L。红外、核磁、质谱以及荧光光谱研究表明,HClO可氧化探针的HC=N键,生成了醛基化合物,发射绿色的荧光。此外,探针还成功应用于可裸眼识别的HClO试纸和复杂水样的加标回收实验。该探针制备简单、响应快、稳定性及特异性强,可作为实际应用中一种有效的次氯酸检测工具。

关键词：次氯酸荧光探针苯并噻唑

SARS-CoV-2 immunity and functional recovery of COVID-19 patients 1-year after infection

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Signal Transduction and Targeted Therapy 2021年第11期6卷 3283-3294页

作者： Yan Zhan Yufang Zhu Shanshan Wang Shijun Jia yunling gao Yingying Lu Caili Zhou Ran Liang Dingwen Sun Xiaobo Wang Zhibing Hou Qiaoqiao Hu Peng Du Hao Yu Chang Liu Miao Cui Gangling Tong Zhihua Zheng Yunsheng Xu Linyu Zhu Jin Cheng Feng Wu Yulan Zheng Peijun Liu Peng Hong Department of Rehabilitation Medicine Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceXiangyangHubei441021China Department of Laboratory Medicine Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceXiangyangHubei441021China Department of Radiology and Medical Imaging Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceXiangyangHubei441021China Department of Research Affairs Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceXiangyangHubei441021China Department of Nephrology Center of Nephrology and UrologySun Yat-sen University Seventh HospitalShenzhenGuangdong518107China Department of Biomedical Science Shenzhen Research InstituteCity University of Hong KongKowloon TongHong KongChina Department of Rehabilitation Medicine Xiangzhou District People’s HospitalXiangyangHubei441000China Department of Rehabilitation Medicine Gucheng People’s HospitalAffiliated Gucheng Hospital of Hubei University of Arts and ScienceXiangyangHubei441700China Division of Quality Control Xiangyang Central Blood StationXiangyangHubei441000China Department of Respiratory and Critical Care Medicine Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceXiangyangHubei441021China Department of Pathology Mount Sinai St.Luke’s Roosevelt Hospital CenterNew YorkNY10025USA Department of Oncology Peking University Shenzhen HospitalShenzhenGuangdong518036China Department of Dermatology Sun Yat-sen University Seventh HospitalShenzhenGuangdong518107China Division of Research and Development US Department of Veterans Affairs New York Harbor Healthcare SystemBrooklynNY11209USA Department of Cell Biology State University of New York Downstate Health Sciences UniversityBrooklynNY11203USA

The long-term immunity and functional recovery after SARS-CoV-2 infection have implications in preventive measures and patient quality of *** we analyzed a prospective cohort of 121 recovered COVID-19 patients from Xiangyang,China at 1-year after *** them,chemiluminescence immunoassay-based screening showed 99%(95%CI,98–100%)seroprevalence 10–12 months after infection,comparing to 0.8%(95%CI,0.7–0.9%)in the general *** anti-receptor-binding domain(RBD)antibodies remained stable since discharge,while anti-RBD IgG and neutralization levels decreased over time.A predictive model estimates 17%(95%CI,11–24%)and 87%(95%CI,80–92%)participants were still 50%protected against detectable and severe re-infection of WT SARS-CoV-2,respectively,while neutralization levels against B.1.1.7 and B.1.351 variants were significantly *** non-severe patients showed normal chest CT and 21%reported COVID-19-related *** contrast,53%severe patients had abnormal chest CT,decreased pulmonary function or cardiac involvement and 79%were still *** findings suggest long-lasting immune protection after SARS-CoV-2 infection,while also highlight the risk of immune evasive variants and long-term consequences for COVID-19 survivors.

关键词： immunity patients infection

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分子印迹荧光传感器研究进展

化学通报 2010年第5期73卷 396-403页

作者：王惠芸高云玲姚克俭浙江工业大学化学工程与材料学院绿色合成技术国家重点实验室培育基地杭州310014

分子印迹技术是结合高分子化学、分析化学、材料科学等发展起来的一门边缘学科,是模拟受体-抗体相互作用的一种新技术。分子印迹荧光传感器结合了分子印迹聚合物的预定识别性和高选择性以及荧光检测的高灵敏性,成为传感领域的研究热点... 详细信息

分子印迹技术是结合高分子化学、分析化学、材料科学等发展起来的一门边缘学科,是模拟受体-抗体相互作用的一种新技术。分子印迹荧光传感器结合了分子印迹聚合物的预定识别性和高选择性以及荧光检测的高灵敏性,成为传感领域的研究热点。本文主要介绍了分子印迹荧光传感器的研究进展,重点概述了分子印迹荧光传感器的制备原理、检测方式及其在有机小分子和离子检测中的应用,并对其发展前景进行了展望。

关键词：分子印迹技术荧光荧光传感器

氮杂氟硼荧(Aza-BODIPY)类荧光探针的研究进展

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化工进展 2018年第3期37卷 1137-1144页

作者：池雨高云玲潘勇刘孟赵辉浙江工业大学化学工程学院浙江杭州310014

氮杂氟硼荧(Aza-BODIPY)作为BODIPYs改良型荧光团,具有摩尔吸光系数高、荧光光谱半峰宽窄、荧光寿命长、光稳定性好、荧光可调等优点,是优良的近红外比色及荧光探针候选母体,然而Aza-BODIPY也存在水溶性能差、应用领域待扩展等问题。本... 详细信息

氮杂氟硼荧(Aza-BODIPY)作为BODIPYs改良型荧光团,具有摩尔吸光系数高、荧光光谱半峰宽窄、荧光寿命长、光稳定性好、荧光可调等优点,是优良的近红外比色及荧光探针候选母体,然而Aza-BODIPY也存在水溶性能差、应用领域待扩展等问题。本文主要综述了近年来各类以Aza-BODIPY为荧光团的探针种类及其应用:首先,简要概括了3种通用的Aza-BODIPY合成方法,包括从查尔酮出发合成Aza-BODIPY的O'Shea法、从2,4-二取代吡咯出发的Carreira法和Lukyanets的邻苯二腈与苯基溴化镁反应的稠环合成法;然后重点对p H、H_2O_2、NH_4^+、F~–、Hg^(2+)、CN~–、蛤毒素和Cys等探针的客体识别性能、识别机理和实际应用进行了总结。提出目前以Aza-BODIPY为母体设计合成的探针数量仍较少,将来的发展方向是通过结构修饰或改造来提高探针的水溶性能、拓宽其激发发射波长到近红外区、增加识别客体的种类和范围,在生物、环境等领域将具有重要的发展前景。

关键词：氮杂氟硼荧荧光探针识别生物检测