Surface molecularly imprinted polymers for solid-phase extraction of （-）-epigallocatechin gallate from toothpaste

作     者：Yunling Gao Ying Hu Kejian Yao 

作者机构：State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology College of Chemical Engineering Zhejiang University of Technology Hangzhou 310032 China 

出 版 物：《Frontiers of Chemical Science and Engineering》 (化学科学与工程前沿（英文版）)

年 卷 期：2015年第9卷第4期

页      面：467-478页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 09[农学] 090203[农学-茶学] 0703[理学-化学] 0902[农学-园艺学] 

基　　金：Acknowledgements This work was supported by Zhejiang Provincial Natural Science Foundation of China (Grant No. LY 12B07010) and National Natural Science Foundation of China (Grant No.20807037) 

主　　题：β-cyclodextrin (-)-epigallocatechin gallate,surface molecular imprinting solid-phase extraction 

摘      要：Surface molecularly imprinted polymers （SMIPs） have been synthesized to selectively determine （-）-epigallocatechin gallate in aqueous media. SMIPs were prepared using a surface grafting copolymerization method on a functionalized silica gel modified with β-cyclodextrin and vinyl groups. The morphology and composition of the SMIPs were investigated by scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analysis. In addition, the molecular binding capacity, recognition properties and selectivity of the SMIPs were evaluated. The imprinted polymers were found to have a highly specific recognition and binding capacity for aqueous media which is （-）-epigallocatechin gallate in the result of the hydrophobic properties of the β-cyclodextrin and the hydrogen-bonding interactions of methacrylic acid. The SMIPs were successfully employed as solid-phase extraction adsor- bents prior to the HPLC determination of （-）-epigallocatechin gallate in toothpaste. The HPLC analysis had a linear dynamic range of 0.5-50.0 μg·mL^-1 with a correlation coefficient of 0.9998 and the recoveries ranged from 89.4% to 97.0% with relative standard deviations less than 4.8%. The limit of detection and limit of quantification were 0.17 and 0.33 μg·mL^-1, respectively. The method provides a promising approach for the preparation of selective materials for the purification and determination of complex samples.