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Signal Transduction and Targeted Therapy 2022年第3期7卷 607-609页

作者： Yuxin Shu Nanfei Yang Nan Cheng Zhengyun Zou\Wenlong Zhang yuncheng bei Qian Shi Menghao Qin Wei-Guo Zhu Pingping Shen State Key Laboratory of Pharmaceutical Biotechnology and The Comprehensive Cancer Center Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolSchool of Life SciencesNanjing UniversityNanjing 210023PR Chino Guangdong Key Laboratory of Genome Instability and Human Disease Shenzhen University International Cancer CenterDepartment of Biochemistry and Molecular BiologyShenzhen UniversityShenzhen 518060PR China Department of Cellular and Integrative Physiology The University of Texas Health Science Center at San Antonio7703 Floyd Curl DriveSan AntonioTX 78229-3904USA

Dear Editor,Tumor-associated macrophages(TAMs)are critical pro-tumor immunocytes and depletion of TAMs has been exploited for cancer therapy.1 However,the phenotypes and functions of TAMs are plastic,TAMs can also be effector cells by engulfing tumor cells and recruiting cytotoxic T cells,thus shaping the actions of TAMs is more scientific rational than depleting them indiscriminately.2 To promote the entry of reorienting TAMs into the clinical treatments of tumors,it is essential to explore the underline mechanisms controlling the anti-and pro-tumor activities of TAMs.

关键词： strengthening clinical rational

A novel mesenchymal stem cell-based regimen for acute myeloid leukemia differentiation therapy

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药学学报:英文版 2023年第7期13卷 3027-3042页

作者： Luchen Sun Nanfei Yang Bing Chen yuncheng bei Zisheng Kang Can Zhang Nan Zhang Peipei Xu Wei Yang Jia Wei Jiangqiong Ke Weijian Sun Xiaokun Li Pingping Shen The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University Wenzhou 325027China School of Pharmaceutical Science Wenzhou Medical UniversityWenzhou 325035China Department of Urology Drum Tower HospitalMedical School of Nanjing UniversityInstitute of UrologyNanjing UniversityNanjing 210008China The Comprehensive Cancer Centre of Nanjing Drum Tower Hospital the Affiliated Hospital of Nanjing University Medical SchoolNanjing UniversityNanjing 210008China Department of Hematology the Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210093China State Key Laboratory of Natural Medicines and Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases Center of Drug DiscoveryChina Pharmaceutical UniversityNanjing 210009China Centre of Micro/Nano Manufacturing Technology(MNMT-Dublin) School of Mechanical&Materials EngineeringUniversity College DublinDublin 4Ireland Department of Surgery Division of Cancer Biology and TherapeuticsCedars-Sinai Medical CenterLos AngelesCA 90048USA Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)&School of Pharmaceutical ScienceWenzhou Medical UniversityWenzhou 325035China Shenzhen Research Institute of Nanjing University Shenzhen 518000China

Currently the main treatment of acute myeloid leukemia(AML)is chemotherapy combining hematopoietic stem cell transplantation.However,the unbearable side effect of chemotherapy and the high risk of life-threatening infections and disease relapse following hematopoietic stem cell transplantation restrict its application in clinical practice.Thus,there is an urgent need to develop alternative therapeutic tactics with significant efficacy and attenuated adverse effects.Here,we revealed that umbilical cord-derived mesenchymal stem cells(UC-MSC)efficiently induced AML cell differentiation by shuttling the neutrophil elastase(NE)-packaged extracellular vesicles(EVs)into AML cells.Interestingly,the generation and release of NE-packaged EVs could be dramatically increased by vitamin D receptor(VDR)activation in UC-MSC.Chemical activation of VDR by using its agonist 1a,25-dihydroxyvitamin D3 efficiently enhanced the pro-differentiation capacity of UC-MSC and then alleviated malignant burden in AML mouse model.Based on these discoveries,to evade the risk of hypercalcemia,we synthetized and identified sw-22,a novel non-steroidal VDR agonist,which exerted a synergistic prodifferentiation function with UC-MSC on mitigating the progress of AML.Collectively,our findings provided a non-gene editing MSC-based therapeutic regimen to overcome the differentiation blockade in AML.

关键词： Mesenchymal stem cell Acute myeloid leukemia Extracellular vesicles Nonsteroidal VDR modulators Combination therapy

二甲双胍脂质体靶向增强TAMs糖摄取对B16-TAMs共培养体系糖分布的影响

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中国细胞生物学学报 2022年第5期44卷 836-847页

作者：郭婉秦文涛贝云成张冬梅南京大学生命科学学院南京210023 中国药科大学天然药物活性组分与药效国家重点实验室南京210009

该文探讨了二甲双胍脂质体(metformin liposome,Met-lipo)通过靶向增强肿瘤相关巨噬细胞(tumor-associated macrophages,TAMs)的葡萄糖摄取以改变小鼠黑色素瘤细胞株B16与TAMs共培养体系中葡萄糖分布,以及抑制B16细胞增殖与迁移的可能... 详细信息

该文探讨了二甲双胍脂质体(metformin liposome,Met-lipo)通过靶向增强肿瘤相关巨噬细胞(tumor-associated macrophages,TAMs)的葡萄糖摄取以改变小鼠黑色素瘤细胞株B16与TAMs共培养体系中葡萄糖分布,以及抑制B16细胞增殖与迁移的可能性。利用2-NBDG作为葡萄糖类似物模拟细胞对葡萄糖的摄取;接着通过2-NBDG摄取实验、葡萄糖/乳酸试剂盒检测二甲双胍(metformin,Met)对TAMs葡萄糖摄取的影响,以及经Met预处理的TAMs改变B16-TAMs共培养体系中葡萄糖分布的能力。用细胞划痕实验、细胞增殖实验、q-PCR实验评价Met预处理的TAMs抑制B16细胞增殖与迁移的能力。同时,通过硫酸铵梯度法制备了Met-lipo,并对其粒径、电势、稳定性、包封率进行表征。通过2-NBDG摄取实验、细胞划痕实验、细胞增殖实验、q-PCR实验检测Met-lipo改变B16-TAMs接触共培养体系中葡萄糖分布的能力和其抗肿瘤效果。结果显示,Met(10 mmol/L)的处理能够有效增强TAMs的糖摄取效率,并实现对B16细胞的葡萄糖剥夺。而制备得到的Met-lipo能特异性地增强B16-TAMs共培养体系中TAMs的糖摄取能力,并展示出远强于游离Met的抗肿瘤效应。综上,Met-lipo能够靶向增强TAMs葡萄糖摄取从而改变B16-TAMs共培养体系的葡萄糖分布,导致B16增殖与迁移能力的下降。

关键词：肿瘤相关巨噬细胞二甲双胍糖摄取脂质体

蒿甲醚联合依托泊苷对小细胞肺癌细胞株H446增殖及侵袭的抑制作用

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中国药科大学学报 2017年第2期48卷 201-207页

作者：陈佳佳贝云成张冬梅沈萍萍南京大学生命科学院南京210093

探讨蒿甲醚与依托泊苷联用对小细胞肺癌细胞H446增殖及侵袭能力的抑制作用。采用MTT法检测蒿甲醚、依托泊苷单用及联合作用对H446细胞增殖的影响;采用Annexin V/PI荧光双染法检测蒿甲醚、依托泊苷单用及联合作用对H446细胞凋亡的影响;采... 详细信息

探讨蒿甲醚与依托泊苷联用对小细胞肺癌细胞H446增殖及侵袭能力的抑制作用。采用MTT法检测蒿甲醚、依托泊苷单用及联合作用对H446细胞增殖的影响;采用Annexin V/PI荧光双染法检测蒿甲醚、依托泊苷单用及联合作用对H446细胞凋亡的影响;采用PI单染法检测蒿甲醚、依托泊苷单用及联合作用对H446细胞周期分布的影响。采用基质胶侵袭实验检测蒿甲醚、依托泊苷单用及联合作用对H446侵袭能力的影响。结果表明,蒿甲醚、依托泊苷单用以及联合使用均能有效抑制H446的增殖,且呈剂量依赖性,两药联用具有协同作用。蒿甲醚单用不能明显促进细胞凋亡,但与依托泊苷(300 nmol/L)联用72 h可以比较明显促进H446凋亡,具有统计学意义(P<0.05)。蒿甲醚单用对H446的细胞周期没有显著影响,联合作用48 h对细胞周期G_2期阻滞主要是依托泊苷的作用。单用蒿甲醚、依托泊苷以及二者联合使用都能显著抑制H446的侵袭能力。

关键词：蒿甲醚依托泊苷小细胞肺癌联合用药增殖侵袭

生物炭对西瓜连作土壤真菌群落结构和功能类群的影响

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环境科学 2024年第6期45卷 3553-3561页

作者：常芳娟张贵云张丽萍吕贝贝刘珍范巧兰姚众山西农业大学棉花研究所运城044000

为明确生物炭施入后对西瓜连作土壤真菌群落结构及功能类群的长期影响,以西瓜成熟期根区土壤为研究对象,借助Illumina Nova Seq高通量测序技术和FUNGuild平台,探究不同用量(7.5、15.0和30.0 t·hm^(-2))生物炭施用3 a后西瓜连作土壤真... 详细信息

为明确生物炭施入后对西瓜连作土壤真菌群落结构及功能类群的长期影响,以西瓜成熟期根区土壤为研究对象,借助Illumina Nova Seq高通量测序技术和FUNGuild平台,探究不同用量(7.5、15.0和30.0 t·hm^(-2))生物炭施用3 a后西瓜连作土壤真菌群落组成、多样性及功能的差异,并分析土壤环境因子与真菌群落结构间的关联性.结果表明,添加生物炭3 a后提高了土壤p H、有效磷、速效钾、全氮、有机质和阳离子交换量,降低了土壤碱解氮含量.生物炭的施用改变了西瓜连作土壤真菌群落结构,提高了土壤真菌的丰富度和多样性.所有土壤样本共获得922个OTU,子囊菌门、担子菌门、被孢菌门、壶菌门和球囊菌门为优势真菌类群,占土壤真菌群落的85.70%~92.45%,生物炭的施入降低了子囊菌门和担子菌门的相对丰度,增加了被孢菌门和球囊菌门的相对丰度;属水平上,随着生物炭的施用,被孢霉属和囊根壶菌属的相对丰度增加,而镰刀菌属相对丰度降低.Mantel检验分析发现,速效钾、碱解氮、有机质和p H是影响土壤真菌群落结构的主要环境因子.真菌主要营养类型为腐生营养型、病理营养型和共生营养型,中、高剂量生物炭处理显著降低了土壤中病理营养型真菌的相对丰度,显著增加了共生营养型真菌的相对丰度.综上,生物炭施用3 a后可改变西瓜连作土壤理化性质,促进土壤真菌群落结构和功能类群向健康有益方向发展.

关键词：生物炭西瓜连作土壤真菌群落真菌功能

Relationship between rock uniaxial compressive strength and digital core drilling parameters and its forecast method

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International Journal of Coal Science & Technology 2021年第4期8卷 605-613页

作者： Hongke Gao Qi Wang bei Jiang Peng Zhang Zhenhua Jiang Yue Wang Geotechnical and Structural Engineering Research Center Shandong UniversityJinan 250061China State Key Laboratory for GeoMechanics and Deep Underground Engineering China University of Mining and TechnologyXuzhou 221116China State Key Laboratory for GeoMechanics and Deep Underground Engineering China University of Mining and TechnologyBeijing 100083China Shandong Tianqin Mining Machinery Equipment Co. Ltd.Yuncheng 274700China

The rock uniaxial compressive strength(UCS)is the basic parameter for support designs in underground engineering.In particular,the rock UCS should be obtained rapidly for underground engineering with complex geological conditions,such as soft rock,fracture areas,and high stress,to adjust the excavation and support plan and ensure construction safety.To solve the problem of obtaining real-time rock UCS at engineering sites,a rock UCS forecast idea is proposed using digital core drilling.The digital core drilling tests and uniaxial compression tests are performed based on the developed rock mass digital drilling system.The results indicate that the drilling parameters are highly responsive to the rock UCS.Based on the cutting and fracture characteristics of the rock digital core drilling,the mechanical analysis of rock cutting provides the digital core drilling strength,and a quantitative relationship model(CDP-UCS model)for the digital core drilling parameters and rock UCS is established.Thus,the digital core drilling-based rock UCS forecast method is proposed to provide a theoretical basis for continuous and quick testing of the surrounding rock UCS.

关键词： Digital core drilling Mechanical analysis Rock UCS Quantitative relationship model Forecast method

Comparative study of model tests on automatically formed roadway and gob-side entry driving in deep coal mines

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International Journal of Mining Science and Technology 2021年第4期31卷 591-601页

作者： Qi Wang Manchao He Shucai Li Zhenhua Jiang Yue Wang Qian Qin bei Jiang State Key Laboratory for Geo-mechanics and Deep Underground Engineering China University of Mining and Technology-BeijingBeijing 100083China Research Institute of Deep Underground Science and Engineering China University of Mining and Technology-BeijingBeijing 100083China Research Center of Geotechnical and Structural Engineering Shandong UniversityJinan 250061China Shandong Tianqin Mining Machinery Equipment Co. LtdYuncheng 274700China

Automatically formed roadway(AFR)by roof cutting with bolt grouting(RCBG)is a new deep coal mining technology.By using this technology,the broken roadway roof is strengthened,and roof cutting is applied to cut off stress transfer between the roadway and gob to ensure the collapse of the overlying strata.The roadway is automatically formed owing to the broken expansion characteristics of the collapsed strata and mining pressure.Taking the Suncun Coal Mine as the engineering background,the control effect of this new technology on roadways was studied.To compare the law of stress evolution and the surrounding rock control mechanisms between AFR and traditional gob-side entry driving,a comparative study of geomechanical model tests on the above methods was carried out.The results showed that the new technology of AFR by RCBG effectively reduced the stress concentration of the roadway compared with gob-side entry driving.The side abutment pressure peak of the solid coal side was reduced by 24.3%,which showed an obvious pressure-releasing effect.Moreover,the position of the side abutment pressure peak was far from the solid coal side,making it more beneficial for roadway stability.The deformation of AFR surrounding rock was also smaller than the deformation of the gob-side entry driving by the overload test.The former was more beneficial for roadway stability than the latter under higher stress conditions.Field application tests showed that the new technology can effectively control roadway deformation.Moreover,the technology reduced roadway excavation and avoided resource waste caused by reserved coal pillars.

关键词： Automatically formed roadway Roof cutting Bolt grouting Roadway control Model test

Al-Zn-Mg-Cu系超高强度铝合金的研究进展

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材料导报 2018年第A2期32卷 358-364页

作者：燕云程黄蓓李维俊卿培林何兵百色学院材料科学与工程学院百色533000

Al-Zn-Mg-Cu系超高强度铝合金具有低密度、高比强度、高韧性和良好的抗腐蚀性能的特点,广泛应用于航空航天、交通运输和兵器领域。本文主要介绍近年来国内外Al-Zn-Mg-Cu系超高强度铝合金的最新研究进展。超高强度铝合金基体上分布着纳... 详细信息

Al-Zn-Mg-Cu系超高强度铝合金具有低密度、高比强度、高韧性和良好的抗腐蚀性能的特点,广泛应用于航空航天、交通运输和兵器领域。本文主要介绍近年来国内外Al-Zn-Mg-Cu系超高强度铝合金的最新研究进展。超高强度铝合金基体上分布着纳米级的晶内时效析出相、亚微米级的高温析出相、微米级的结晶析出相和晶界析出相,这些相的形态、数量、尺寸和分布对合金的综合力学性能和抗腐蚀性能有直接的影响;主元素成分含量对超高强度铝合金综合力学性能有影响,合金的综合力学性能随Zn/Mg和Cu/Mg比值的变化而变化;微量元素能够提高超高强度铝合金的综合力学性能。微量元素对铝合金的影响主要体现在提高沉淀相的过饱和度,改变沉淀析出过程,促进或抑制沉淀相的析出和促进新相的沉淀析出。新制备技术能够显著细化晶粒、抑制偏析、析出相均匀分布和提高各种元素的过饱和度,从而改善超高强度铝合金的综合力学性能。强化固溶处理能够提高时效析出程度,从而提高铝合金的力学性能。三级时效处理后的超高强度铝合金具有峰值时效T6态的强度和优异的抗腐蚀性能。

关键词：超高强度铝合金微观组织力学性能腐蚀性能热处理

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基于机器视觉的安全距离检测方法

山东电力技术 2021年第1期48卷 27-31页

作者：裴云成贝前程刘海英张绍杰齐鲁工业大学(山东省科学院)电气工程与自动化学院山东济南250353

将机器视觉技术应用在距离检测中能更好地提高检测的精度,特别是在某些重要场所和恶劣环境下,机器视觉技术能更好地代替人类工作。将双目视觉技术与图像处理技术相结合,先应用高斯拉普拉斯算子(Laplacian of Gaussian,LOG)对采集的图像... 详细信息

将机器视觉技术应用在距离检测中能更好地提高检测的精度,特别是在某些重要场所和恶劣环境下,机器视觉技术能更好地代替人类工作。将双目视觉技术与图像处理技术相结合,先应用高斯拉普拉斯算子(Laplacian of Gaussian,LOG)对采集的图像进行预处理,获得清晰的边缘信息,然后根据双目立体视觉原理,获取图像边缘点的坐标,最后根据空间点的距离公式获得两物体边缘点距离集合。筛选出其中的最短距离即为两物体间安全距离的判断依据,此方法也可用于输电线路周围障碍物安全距离的检测。

关键词：双目视觉 LOG 摄像机标定立体视觉

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基于改进经典蚁群算法的机器人路径规划

山东电力技术 2020年第11期47卷 24-27页

作者：贝前程裴云成刘海英张绍杰齐鲁工业大学(山东省科学院) 山东济南250353

经典蚁群算法是一种实用的机器人路径规划方法,但前期搜索的时间较长,且参数选择不当时易在搜索的前期陷入全局最优。为了解决该问题,提出适应度函数,并且在不改变蚁群初始参数的情况下,通过重新设计蚁群启发函数,使得改进后的蚁群算法... 详细信息

经典蚁群算法是一种实用的机器人路径规划方法,但前期搜索的时间较长,且参数选择不当时易在搜索的前期陷入全局最优。为了解决该问题,提出适应度函数,并且在不改变蚁群初始参数的情况下,通过重新设计蚁群启发函数,使得改进后的蚁群算法比经典的蚁群算法具有更快的收敛速度以及更短的搜索路径。最后通过MATLAB仿真将改进后的算法和经典的蚁群算法进行对比,验证了改进算法的有效性。

关键词：路径规划蚁群算法启发函数