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Mkrn2 deficiency induces teratozoospermia and male infertility through p53/PERP-mediated apoptosis in testis

Asian Journal of Andrology 2020年第4期22卷 414-421页

作者： ying-Chen Qian yun-Xia Xie Chao-Shan Wang Zhu-Mei Shi Cheng-Fei Jiang yun-yi tang Xu Qian Lin Wang Bing-Hua Jiang Department of Pathology Nanjing Medical UniversityNanjing 210029China Department of Neurosurgery The First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China The Academy of Medical Sciences Zhengzhou UniversityZhengzhou 450001China Department of Pathology The University of IowaIowa CityIA 52242USA

The apoptosis that occurs in the immature testis under physiological conditions is necessary for male germ cell development,whereas improper activation of apoptosis can impair spermatogenesis and cause defects in *** previously demonstrated that in mice,the makorin-2(Mkrn 2)gene is expressed exclusively in the testis and its deletion leads to male *** understand the potential molecular mechanism,in this study,we found that levels of apoptosis in the testis were abnormally high in the absence of Mkrn *** identify specific gene(s)involved,we performed digital gene expression profiling(DGE)and pathway analysis via gene set enrichment analysis(GSEA)and the Kyoto Encyclopedia of Genes and Genomes(KEGG)database,and we found that MKRN2 inhibits p53 apoptosis effector related to PMP22(PERP)expression and that levels of the protein in sperm samples have an inverse correlation with infertility *** additionally indicated that PERP is a negative regulator of spermatogenesis and that its ectopic expression induces male ***,Gene Expression Omnibus(GEO)dataset analysis showed that p53,upstream of PERP,was upregulated in oligoasthenoteratozoospermia(OAT).These observations suggest that Mkrn 2 is crucial for protecting germ cells from excessive apoptosis and implicate Mkrn 2-based suppression of the p53/PERP signaling pathway in spermatogenesis and male fertility.

关键词： apoptosis makorin-2 p53 p53 apoptosis effector related to PMP22 spermatogenesis

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Coordinated Control Strategy of New Energy Power Generation System with Hybrid Energy Storage Unit

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Energy Engineering 2025年第1期122卷 167-184页

作者： yun Zhang ZifenHan Biao Tian Ning Chen yi Fan School of Mechano-Electronic Engineering Xidian UniversityXi’an710071China Electric Power Control Center State Grid Gansu Electric Power CompanyLanzhou730030China Electric Power Control Center State Grid Gansu Electric Power Company Zhangye Power Supply BranchZhangye734000China

The new energy power generation is becoming increasingly important in the power *** as photovoltaic power generation has become a research hotspot,however,due to the characteristics of light radiation changes,photovoltaic power generation is unstable and random,resulting in a low utilization rate and directly affecting the stability of the power *** solve this problem,this paper proposes a coordinated control strategy for a newenergy power generation system with a hybrid energy storage unit based on the lithium iron phosphate-supercapacitor hybrid energy storage ***,the variational mode decomposition algorithm is used to separate the high and low frequencies of the power signal,which is conducive to the rapid and accurate suppression of the power fluctuation of the energy storage ***,the fuzzy control algorithm is introduced to balance the power between energy *** this paper,the actual data is used for simulation,and the simulation results show that the strategy realizes the effective suppression of the bus voltage fluctuation and the accurate control of the internal state of the energy storage unit,effectively avoiding problems such as overshoot and over-discharge,and can significantly improve the stability of the photovoltaic power generation systemand the stability of the Direct Current *** is of great significance to promote the development of collaborative control technology for photovoltaic hybrid energy storage units.

关键词： Photovoltaic power suppression hybrid energy storage unit variationalmodal decomposition fuzzy control power distribution control

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The detection, extraction and parameter estimation of extreme-mass-ratio inspirals with deep learning

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 86-96页

作者： Qianyun yun Wen-Biao Han yi-Yang Guo He Wang Minghui Du Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences Shanghai Astronomical Observatory Chinese Academy of Sciences School of Physics and Astronomy Shanghai Jiao Tong University School of Astronomy and Space Science University of Chinese Academy of Sciences Taiji Laboratory for Gravitational Wave Universe (Beijing/Hangzhou) University of Chinese Academy of Sciences Lanzhou Center for Theoretical Physics Key Laboratory of Theoretical Physics of Gansu Province Key Laboratory of Quantum Theory and Applications of MoE Lanzhou University Institute of Theoretical Physics & Research Center of Gravitation Lanzhou University International Centre for Theoretical Physics Asia-Pacific (ICTP-AP) University of Chinese Academy of Sciences (UCAS) Center for Gravitational Wave Experiment National Microgravity Laboratory Institute of Mechanics Chinese Academy of Sciences

One of the primary goals of space-borne gravitational wave detectors is to detect and analyze extreme-mass-ratio inspirals(EMRIs). This task is particularly challenging because EMRI signals are complex, lengthy, and faint. In this work, we introduce a2-layer convolutional neural network(CNN) approach to detect EMRI signals for space-borne detectors, achieving a true positive rate(TPR) of 96.9% at a 1% false positive rate(FPR) for signal-to-noise ratio(SNR) from 50 to 100. Especially, the key intrinsic parameters of EMRIs such as the mass, spin of the supermassive black hole(SMBH) and the initial eccentricity of the orbit can also be inferred directly by employing a neural network. The mass and spin of the SMBH can be determined at 99% and 92% respectively. This will greatly reduce the parameter spaces and computing cost for the following Bayesian parameter *** model also has a low dependency on the accuracy of the waveform model. This study underscores the potential of deep learning methods in EMRI data analysis, enabling the rapid detection of EMRI signals and efficient parameter estimation.

关键词： gravitational wave EMRIs deep-learing

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Spermidine inhibits high glucose-induced senescence in HT-22 cells by upregulation of CB1 receptor

Spermidine inhibits high glucose-induced senescence in HT-22...

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中国生理学会张锡钧基金会第十四届全国青年优秀生理学学术论文交流及评奖会议、中国生理学会第十二届全国青年生理学工作者学术会议

作者： Wei-Wen Zhu Fan Xiao yi-yun tang Wei Zou Xiang Li Ping Zhang Ai-Ping Wang Xiao-Qing tang Institute of Neuroscience Hunan Province Cooperative Innovation Center for Molecular Target New Drug StudyUniversity of South China Department of Physiology Medical CollegeUniversity of South China Department of Neurology Nanhua Affiliated HospitalUniversity of South China Department of Anesthesiology the First Affiliated HospitalUniversity of South China Department of Anatomy Medical CollegeUniversity of South China

Background:Hyperglycemia induces neuronal senescence that is common in diabetic *** receptor type 1(CB1)protects against *** evidence show that spermidine(Spd)has anti-aging function in many ***,the role of Spd in hyp... 详细信息

关键词： Spermidine inhibits high glucose-induced senescence in HT-22 cells by upregulation of CB1 receptor

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Hydrogen Sulfide Enhances Hippocampal Autophagic Flux to Inhibit Cognitive Impairment in Streptozotocin-induced Diabetic Rats by Up-regulation of Ornithine Decarboxylase/Spermidine Pathway

Hydrogen Sulfide Enhances Hippocampal Autophagic Flux to Inh...

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中国生理学会张锡钧基金会第十四届全国青年优秀生理学学术论文交流及评奖会议、中国生理学会第十二届全国青年生理学工作者学术会议

作者： Cheng Li Yan Xie Ling-Li He yi-yun tang Xiang Li Wei Zou Ai-Ping Wang Ping Zhang Xiao-Qing tang Department of Neurology the Second Affiliated HospitalUniversity of South China Department of Physiology Medical CollegeUniversity of South China Department of Anesthesiology the First Affiliated HospitalUniversity of South China Department of Neurology Nanhua Affiliated HospitalUniversity of South China

We have previously demonstrated the improving role of hydrogen sulfide（HS）,the third gasotransmitter,in the cognitive function of streptozotocin（STZ）-induced diabetic *** has been confirmed that impaired hippocampal autophagic flux has a key role in the pathogenesis of cognitive impairment and that ornithine decarboxylase（ODC）/spermidine（Spd） pathway plays an important role in the formation of memory by promoting autophagic ***,we investigated the roles of hippocampal ODC/Spd pathway and autophagic flux in HS-attenuated cognitive impairment in streptozotocin（STZ）-induced diabetic *** this study,we found that sodium hydrosulfide（NaHS,a donor of HS） up-regulated the expression of ODC and the level of Spd in the hippocampus of STZ-induced diabetic *** also found that NaHS markedly improved the autophagic flux in the hippocampus of STZ-exposed rats,as evidenced by the increases in the numbers of autophagosomes as wells as the expressions of LC3-Ⅱ,Beclin-1,and P62 in the hippocampus of cotreatment with NaHS and STZ ***,difluoromethylomithine（DFMO,an inhibitor of ODC） markedly prevented the up-regulated role of NaHS in the level of hippocampal Spd and reversed the protection of NaHS against the impairments in hippocampal autophagic flux as well as cognitive function in STZ-exposed *** findings indicated that improving hippocampal autophagic flux plays a key role in HS-attenuated cognitive impairment in STZ-induced diabetic rats,which is mediated by up-regulation of hippocampal ODC/Spd pathway.

关键词： autophagic flux diabetes-induced cognitive impairment hippocampal ornithine decarboxylase/spermidine pathway hydrogen Sulfide sodium hydrosulfide

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Role of Silent Information Regulator 1 in the Protective Effect of Hydrogen Sulfide on Homocysteine-Induced Cognitive Dysfunction:Involving Reduction of Hippocampal ER Stress

Role of Silent Information Regulator 1 in the Protective Eff...

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中国生理学会张锡钧基金会第十四届全国青年优秀生理学学术论文交流及评奖会议、中国生理学会第十二届全国青年生理学工作者学术会议

作者： yi-yun tang Ai-Ping Wang Hai-Jun Wei Man-Hong Li Wei Zou Xiang Li Chun-Yan Wang Ping Zhang Xiao-Qing tang Department of Physiology Medical CollegeUniversity of South China Department of Anatomy Medical CollegeUniversity of South China Department of Neurology Nanhua Affiliated HospitalUniversity of South China Department of Anesthesiology the First Affiliated HospitalUniversity of South China Department of Pathophysiology Medical CollegeUniversity of South China

Background:Our previous study has confirmed that Hydrogen sulfide（HS）,a new gaseous signaling molecule,attenuates homocysteine（Hcy）-induced cognitive ***,the underlying mechanism remains largely *** information regulator 1（Sirt-1） is indispensable in the formation of learning and ***,the aim of this study was to explore the role of Sirt-1 in the protective effect of HS against Hcy-induced cognitive ***:The cognitive behaviors were detected by the Morris water maze（MWM） test and the novel objects recognition test（NORT）.The expression of Sirt-1,glucose regulated protein 78（GRP78）,C/EBP homologous protein（CHOP） and cleaved caspase-12 in the hippocampus were examined by Western ***:NaHS（a donor of HS） markedly up-regulated the expression of Sirt-1 in the hippocampus of Hcy-exposed ***,a specific inhibitor of Sirt-1,reversed the improving role of NaHS in the cognitive function of Hcy-exposed rats,as evidenced by that sirtinol increased the escape latency and the swim distance in the acquisition trial of MWM test,decreased the times crossed through and the time spent in the target quadrant in the probe trail of MWM test,and reduced the discrimination index in the NORT in the rats cotreated with NaHS and *** also found that sirtinol reversed the protection of NaHS against Hcy-induced hippocampal ER-stress,as evidenced by up-regulating the expressions of GRP78,CHOP,and cleaved caspase-12 in the hippocampus of rats cotreated with NaHS and ***:These results suggested the contribution of upregulation of hippocampal Sirt-1 to the improving role of HS in the cognitive function of Hcy-exposed rats,which involves suppression of hippocampal ER *** finding provides a new insight into the mechanism underlying the inhibitory role of HS in Hcy-induced cognitive dysfunction.

关键词： Hydrogen sulfide Homocysteine Silent mating type information regulation 2 homolog 1 endoplasmic reticulum stress cognitive dysfunction

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BDNF/TrkB pathway mediates the antidepressant-like role of H2S in CUMS-exposed rats by inhibition of hippocampal ER stress

BDNF/TrkB pathway mediates the antidepressant-like role of H...

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中国生理学会张锡钧基金会第十四届全国青年优秀生理学学术论文交流及评奖会议、中国生理学会第十二届全国青年生理学工作者学术会议

作者： Le Wei Li-Yuan kan Hai-ying Zeng yi-yun tang Hong-Lin Huang Ming Xie Wei Zou Chun-Yan Wang Ping Zhang Xiao-Qing tang Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study Institute of Pharmacy and PharmacologyUniversity of South China Institute of Neuroscience Medical CollegeUniversity of South China Department of Neurology the First Affiliated HospitalUniversity of South China Department of Neurology Nanhua Affiliated HospitalUniversity of South China Department of Pathophysiology Medical CollegeUniversity of South China

Accumulating evidences suggest that chronic unpredictable mild stress（CUMS）induced depressive-like behaviors and hippocampal endoplasmic reticulum（ER）*** previous work showed that Hydrogen sulfide（HS）significant... 详细信息

关键词： BDNF/TrkB pathway mediates the antidepressant-like role of H2S in CUMS-exposed rats by inhibition of hippocampal ER stress

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Maintaining moderate levels of hypochlorous acid promotes neural stem cell proliferation and differentiation in the recovery phase of stroke

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Neural Regeneration Research 2025年第3期20卷 845-857页

作者： Lin-Yan Huang yi-De Zhang Jie Chen Hai-Di Fan Wan Wang Bin Wang Ju-yun Ma Peng-Peng Li Hai-Wei Pu Xin-yian Guo Jian-Gang Shen Su-Hua Qi School of Medical Technology Xuzhou Key Laboratory of Laboratory DiagnosticsXuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Pharmacy Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Laboratory Medicine Branch Hospital of Huai’an First People’s HospitalHuai’anJiangsu ProvinceChina Department of Laboratory Medicine the Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Chinese Medicine The University of Hong KongHong Kong Special Administrative RegionChina

It has been shown clinically that continuous removal of ischemia/reperfusion-induced reactive oxygen species is not conducive to the recovery of late ***,previous studies have shown that excessive increases in hypochlorous acid after stroke can cause severe damage to brain *** previous studies have found that a small amount of hypochlorous acid still exists in the later stage of stroke,but its specific role and mechanism are currently *** simulate stroke in vivo,a middle cerebral artery occlusion rat model was established,with an oxygen-glucose deprivation/reoxygenation model established in vitro to mimic *** found that in the early stage(within 24 hours)of ischemic stroke,neutrophils produced a large amount of hypochlorous acid,while in the recovery phase(10 days after stroke),microglia were activated and produced a small amount of hypochlorous ***,in acute stroke in rats,hypochlorous acid production was prevented using a hypochlorous acid scavenger,taurine,or myeloperoxidase inhibitor,4-aminobenzoic acid *** results showed that high levels of hypochlorous acid(200μM)induced neuronal apoptosis after oxygen/glucose deprivation/***,in the recovery phase of the middle cerebral artery occlusion model,a moderate level of hypochlorous acid promoted the proliferation and differentiation of neural stem cells into neurons and *** suggests that hypochlorous acid plays different roles at different phases of cerebral ischemia/reperfusion *** levels of hypochlorous acid(5 and 100μM)promoted nuclear translocation ofβ-*** transfection of single-site mutation plasmids,we found that hypochlorous acid induced chlorination of theβ-catenin tyrosine 30 residue,which promoted nuclear ***,our study indicates that maintaining low levels of hypochlorous acid plays a key role in the recovery of neurological function.

关键词： cell differentiation cerebral ischemia/reperfusion injury chlorination hypochlorous acid microglia neural stem cell neurogenesis nuclear translocation stroke β-catenin

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Deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

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Science China Chemistry 2025年第01期 288-296页

作者： Shuai Liu Wen-Qiang Xu ying-Tong Di Man-Cheng tang Ding-Kang Chen Ming-Ming Cao Yao-Wen Chang Hong-Yu tang Chun-Mao Yuan Jun-Bo Yang Zhi-Li Zuo Han Guo Zi-Fei Xu ying Zeng yun-Dong Wu Xiao-Jiang Hao Key Laboratory of Phytochemistry and Natural Medicines (Chinese Academy of Sciences) Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences Lab of Computational Chemistry and Drug Design State Key Laboratory of Chemical Oncogenomics Peking University Shenzhen Graduate School Shenzhen Bay Laboratory State Key Laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical University Molecular Biology Experimental Center Germplasm Bank of Wild Species in Southwest China Kunming Institute of Botany Chinese Academy of Sciences yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Research Unit of Chemical Biology of Natural Anti-Virus Products Chinese Academy of Medical Sciences

In the intricate process of natural product biosynthesis, a metabolon can enhance metabolic flux by associating sequential enzymes. A fungal metabolon, comprising of flavin-dependent monooxygenase Spe F and P450 monooxygenase Spe G, is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide B. Using notoamide E as a substance, Spe F/Spe G metabolon can control the stereoselectivity of its 2,3-epoxidation, followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-m A with dienyl iminium unit. Subsequently,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol rearrangement. This provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

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[Determination of 12 halogenated organic pollutants in edible fish by ultra performance liquid chromatography-high resolution mass spectrometry combined with ultrasound-assisted extraction and gel permeation chromatography purification]

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Se pu = Chinese journal of chromatography 2025年第1期43卷 68-77页

作者： yi-Zhe Zhu Rui-Fen Zheng Zi-Hao Fan Ling Liu Jing-Yao Ye Kai Wang Cai-Ming tang Research Center for Eco-Environmental Engineering Dongguan University of Technology Dongguan 523808 China. Guangdong Key Laboratory of Environmental Resources Utilization and Protection Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China.

Halogenated organic pollutants (HOPs) have attracted considerable attention owing to their persistence, bioaccumulation, and toxicity. The development of methods to detect HOPs in fish is challenging owing to the compositional complexity of fish matrices, which contain high levels of lipids and relatively low concentrations of HOPs. In addition, the lipophilicity of most HOPs renders their extraction difficult. Moreover, the simultaneous determination of multiple HOPs to achieve the high-throughput screening of these analytes is complex. In this study, a reliable and efficient pretreatment method based on ultrasound-assisted extraction, gel permeation chromatography purification, and ultra performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) was developed for the determination of 12 HOPs in edible fish. The procedures of sample extraction and purification and LC-HRMS detection parameters were optimized to improve the performance of the method. Fresh fish samples were thoroughly rinsed with water, and non-edible parts, including the skin, bones, and phosphorus, were removed. The fish were weighed, cut into small pieces, and vacuum freeze-dried for 48 h. Subsequently, a freeze grinder was used to grind the dried fish into a fine powder. Exactly 2 g of the fish powder was weighed, fortified with isotope-labeled internal standards of the HOPs, and allowed to stand for 5 min. Methanol-acetonitrile (1∶1, v/v) was then added, followed by vortex mixing and ultrasonication. After centrifugation, the supernatant was transferred to a fresh tube. The extraction process was repeated twice and all extracts were combined. The extract was evaporated under a gentle nitrogen flow and redissolved in a mixture of ethyl acetate-cyclohexane (1∶1, v/v). The sample mixture was cleaned using gel permeation chromatography, and the eluate was collected and concentrated under a nitrogen flow. Sample residuals were reconstituted with water-methanol (1∶1, v/v) prior to

关键词： edible fish gel permeation chromatography (GPC) halogenated organic pollutants (HOPs) ultra performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS)

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