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Molecular cloning,characterization and promoter analysis of LbgCWIN1 and its expression profiles in response to exogenous sucrose during in vitro bulblet initiation in lily

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Horticultural Plant Journal 2024年第2期10卷 545-555页

作者： Cong Gao Shiqi li yunchen Xu Yue liu Yiping xia Ziming Ren yun Wu Genomics and Genetic Engineering Laboratory of Ornamental Plants Institute of Landscape ArchitectureCollege of Agriculture and BiotechnologyZhejiang UniversityHangzhouZhejiang 310058China Laboratory of Flower Bulbs Department of Landscape ArchitectureSchool of Civil Engineering and ArchitectureZhejiang Sci-Tech UniversityHangzhouZhejiang 310018China

lily(lilium spp.) is an important ornamental flower, which is mainly propagated by bulbs. Cell wall invertases(CWINs), which catalyze the irreversibly conversion of sucrose into glucose and fructose in the extracellular space, are key enzymes participating in sucrose allocation in higher plants. Previous studies have shown that CWINs play an essential role in bulblet initiation process in bulbous crops, but the underlying molecular mechanism remains unclear. Here, a CWIN gene of lilium brownii var. giganteum(Lbg) was identified and amplified from genomic DNA. Quantitative RT-PCR assays revealed that the expression level of LbgCWIN1 was highly upregulated exactly when the endogenous starch degraded in non-sucrose medium during in vitro bulblet initiation in Lbg. Phylogenetic relationship, motif, and domain analysis of LbgCWIN1 protein and CWINs in other plant species showed that all sequences of these CWIN proteins were highly conserved. The promoter sequence of LbgCWIN1 possessed a number of alpha-amylase-, phytohormone-, light-and stress-responsive cis-elements. Meanwhile, β-glucuronidase(GUS) assay showed that the 459 bp upstream fragment from the translational start site displayed maximal promoter activity. These results revealed that LbgCWIN1 might function in the process of in vitro bulblet initiation and be in the response to degradation of endogenous starch.

关键词： lilium brownii var.giganteum LbgCWIN1 Phylogenetic analysis Promoter analysis Bulblet initiation

Protective effects of CY-09 and astaxanthin on NaIO_(3)-induced photoreceptor inflammation via the NLRP3/autophagy pathway

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International Journal of Ophthalmology(English edition) 2024年第7期17卷 1217-1231页

作者： xiao-li Wang yun-xia Gao Qiong-Zhen Yuan Ming Zhang Department of Ophthalmology West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina

AIM:To study the effect of the NLRP3/autophagy pathway on the photoreceptor inflammatory response and the protective mechanism of CY-09 and astaxanthin(AST).METHODS:ICR mice were intraperitoneally injected NaIO_(3),CY-09,AST successively and divided into 5 groups,including the control,NaIO_(3),NaIO_(3)+CY-09,NaIO_(3)+AST,and NaIO_(3)+CY-09+AST *** domain optical coherence tomography and flash electroretinogram were examined and the retina tissues were harvested for immunohistochemistry,enzyme linked immunosorbent assay(EliSA),and Western *** pigment epithelium cell line(ARPE-19 cells)and mouse photoreceptor cells line(661W cells)were also treated with NaIO_(3),CY-09,and AST *** proliferation was assessed by cell counting kit-8(CCK-8)*** was analyzed by flow *** in autophagosome morphology were observed by transmission electron *** polymerase chain reaction(qPCR)was used to detect NLRP3 and ***3,caspase-1,cleaved caspase-1,p62,Beclin-1,and LC3 protein levels were measured by Western ***-1βand IL-18 were measured by ***:Compared with the control group,the activity of NaIO_(3)-treated 661W cells decreased within 24 and 48h,apoptosis increased,NLRP3,caspase-1,IL-1βand IL-18 levels increased,and autophagy-related protein levels increased(Plin-1,and LC3 in vitro and in vivo(P<0.05).CONCLUSION:CY-09 and AST inhibit NaIO_(3)-induced inflammatory damage through the NLRP3/autophagy pathway in vitro and in ***-09 and AST may protect retina from inflammatory injury.

关键词： CY-09 astaxanthin retinal degeneration photoreceptor cells inflammation NLRP3

Change-point Estimation of a Mean Shift in Moving-average Processes Under Dependence Assumptions

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Acta Mathematicae Applicatae Sinica 2006年第4期22卷 615-626页

作者： yun-xia li Zhejiang University of Finance and Economics Hangzhou 310018 China

In this paper we discuss the least-square estimator of the unknown change point in a mean shift for moving-average processes of ALNQD sequence. The consistency and the rate of convergence for the estimated change point are established. The asymptotic distribution for the change point estimator is obtained. The results are also true for ρ-mixing, φ-mixing, α-mixing sequences under suitable conditions. These results extend those of Bai, who studied the mean shift point of a linear process of i.i.d, variables, and the condition ∑j=0^∞j｜aj｜〈 ∞ in Bai is weakened to ∑j=0^∞｜aj｜〈∞.

关键词： Mean shift moving-average process change point ALNQD rate of convergence

Systematic bibliometric analysis of research hotspots and trends on the application of premium IOLs in the past 2 decades

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International Journal of Ophthalmology(English edition) 2024年第4期17卷 736-747页

作者： liang-Pin li li-yun Yuan De-Shen Mao xia Hua xiao-Yong Yuan Clinical College of Ophthalmology Tianjin Medical UniversityTianjin 300020China Tianjin Key Laboratory of Ophthalmology and Visual Science Tianjin Eye InstituteTianjin Eye HospitalTianjin 300020China School of Medicine Nankai UniversityTianjin 300071China Anhui Medical University Hefei 230032Anhui ProvinceChina Tianjin Aier Eye Hospital Tianjin UniversityTianjin 300190China

AIM:To analysis of research hotspots and trends on the application of premium intraocular lens(PIOLs)in the past 2 ***:The literature search was performed on the Web of Science and included PIOLs studies published between January 2000 and December *** retrieved literature was collated and analyzed by R-tool’s Bibliometrix package,CitNetExplorer,CiteSpace and other ***:A total of 1801 articles about PIOLs were obtained,most of which were published in Spain and the United *** organization that published the most articles was the University of Valencia in ***ó JL,and Montés-Micó R,from Spain were the most influential authors in this *** Journal of Cataract and Refractive Surgery and Journal of Refractive Surgery were the core journals for this field;the top 10 cited articles mainly focus on postoperative satisfaction with multifocal intraocular lens(IOLs)and postoperative results of toric *** the keyword analysis,we found that trifocal IOLs,astigmatism and extended depth of focus(EDoF)IOLs are the most discussed topics at present,and the importance of astigmatism and the clinical application of the new generation of PIOLs are the emerging research ***:Bibliometric analysis can effectively help to identify multilevel concerns in PIOLs research and the prevailing research trends in the realm of PIOLs encompass the adoption of EDoF IOLs,trifocal IOLs,and their respective Toric models.

关键词： premium intraocular lens bibliometric H-index cataract surgery global trends

Effect of lanthanum on inclusions in non-oriented electrical steel slabs

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Journal of Iron and Steel Research(International) 2024年第7期31卷 1680-1691页

作者： Qiang Ren Zhi-yuan Hu yun-xia liu Wei-cheng Zhang Zi-qi Gao li-feng Zhang School of Mechanical Engineering Yanshan UniversityQinhuangdao 066004HebeiChina School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China Research and Development Department Shougang Zhixin Qian'an Electromagnetic Material Co.Ltd.Qian'an 064400HebeiChina School of Mechanical and Materials Engineering North China University of TechnologyBeijing 100144China

The effect of lanthanum on the characteristics of inclusions in the slab of non-oriented electrical steels was investigated through industrial trials and thermodynamic *** number,size,and chemical composition of inclusions in the surface,one-quarter of thickness and the center of slabs with and without lanthanum addition were statistically analyzed using an automatic inclusion analysis *** the lanthanum-free slab,inclusions were predominately ***_(2)O_(3),MgO,and AlN as well as a small number of Al_(2)O_(3)-MgO-CaO and *** number densities of oxide inclusions and AlN decreased from the surface to the center of the slab,which was ascribed to the difference in cooling intensity during the continuous *** the steel with lanthanum addition,inclusions were modified into LaAlO_(3) and La_(2)O_(2)S and gradually transformed into dual-phase MgO-La_(2)S3 with an increasing distance from the slab surface due to the reaction between the lanthanum-containing inclusion and the steel *** uneven distribution of oxide inclusions along the thickness of the slab was eliminated in the lanthanum-bearing slab because the dissolved oxygen was remarkably decreased by ***-bearing inclusions were more likely to agglomerate AlN by inducing the heterogeneous nucleation of AlN on their surface,while small-size ***_(2)O_(3) inclusions hardly showed a coarsening effect on the size of AlN.

关键词： Lanthanum-Inclusion Non-oriented electrical steel Aluminum nitride Heterogenous nucleation

Testing for Changes in the Mean or Variance of Long Memory Processes

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Acta Mathematica Sinica,English Series 2010年第12期26卷 2443-2460页

作者： yun xia li Jian Jun XU li Xin ZHANG School of Mathematics and Statistics Zhejiang University of Finance and Economics Hangzhou 310018 P. R. China Department of Mathematics Zhejiang University Hangzhou 310027 P. R. China

In this paper, we study the asymptotic CUSUM tests for detecting changes in the mean or variance of a moving-average process with long memory. When there is no change over [O,T], the asymptotic distribution of the test statistic is derived, which allows us to find asymptotic critical values. When there is a change, the behavior of the test statistic is discussed. Conditions for the consistency of these tests are also discussed. Based on the asymptotic results, simulation studies of testing for changes in the mean show that the CUSUM test proposed performs well.

关键词： Change-point moving-average process long memory invariance principle fractionalBrownian motion

Absorption characteristics,model,and molecular mechanism of hydrogen sulfide in morpholine acetate aqueous solution

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Chinese Journal of Chemical Engineering 2024年第2期66卷 125-135页

作者： Hongwei Jin yun Teng Kangkang li Zhou Feng Zhonghao li Shiqi Qu Hongzhi xia Huanong Cheng Yugang li Xinshun Tan Shiqing Zheng College of Chemical Engineering Qingdao University of Science and TechnologyQingdao 266042China

The solubility of H_(2)S was measured in solutions of N-butyl-N-methylmorpholine acetate([Bmmorp][Ac])containing 20%-40%(mass)water at experimental temperatures ranged from 298.15 to 328.15 K and pressures up to 320 k *** total solubility of H_(2)S increased with higher temperatures,lower pressures,and reduced water *** reaction equilibrium thermodynamic model was used to correlate the solubility *** results indicate that the chemical reaction equilibrium constant decrease with increasing water content and temperature,whereas Henry constant increase with increasing water content and *** with other ionic liquids,H_(2)S exhibits a higher physical absorption enthalpy and a lower chemical absorption enthalpy in[Bmmorp][Ac]aqueous *** suggests that[Bmmorp][Ac]has a strong physical affinity for H_(2)S and low energy requirement for *** chemical methods were used to investigate the molecular mechanism of H_(2)S absorption in ionic *** interaction energy analysis revealed that the binding of H_(2)S with the ionic liquid in a1:2 ratio is more *** analyses by the methods of the interaction region indicator and the atoms in molecules were conducted to the interactions between H_(2)S and the ionic liquid.

关键词： Ionic liquid Hydrogen sulfide Model Solubility Molecular mechanism

A regularization algorithm of dynamic light scattering for estimating the particle size distribution of dual-substance mixture in water

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Particuology 2024年第6期89卷 246-257页

作者： Yanghong Wang Ziqiang Meng Zipei Zhang Min xia li xia Wei li School of Optical and Electronic Information Huazhong University of Science and TechnologyWuhan 430074China

Dynamic light scattering(DLS)is a nondestructive,well-established technique for the size characterization of proteins,nanoparticles,polymers,and colloidal ***,current DLS techniques are only applied to particle groups of single composition due to the limitation of their inversion *** this study,we propose a particle size distribution inversion algorithm based on the Tikhnonov regularization method that can be applied to the dual-substance particle *** algorithm retrieves the particle size distributions of two substances,respectively,by taking advantage of their refractive index *** simulation results reveal that the algorithm has excellent accuracy and stability when the scattering angle is 30°.Instead of the original identity matrix,the first-order difference matrix and second-order difference matrix are used as the regular matrix when utilizing the Tikhnonov algorithm,which obviously improves the anti-interference,accuracy,and stability of the ***,the inversion of particle size distribution is carried out at a 0.01%–1%noise level,which shows that the algorithm has an available antinoise ***,experimental particle size measurements for a mixture of polystyrene beads and toner particles demonstrate that the proposed algorithm is superior to the traditional Tikhnonov algorithm in applicability and accuracy.

关键词： Dynamic light scattering Dual-substance mixture Particle sized istribution Tikhnonov regularization

X-ray excited Mn^(2+)-doped persistent luminescence materials with biological window emission for in vivo bioimaging

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Journal of Rare Earths 2024年第1期42卷 28-35,I0001页

作者： Hanrun Zheng lin liu Yi’ang li Rui Rong liang Song Junpeng Shi Jing Teng xia Sun yun Zhang State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou350002China University of the Chinese Academy of Sciences Beijing100049China xiamen Hospital of Traditional Chinese Medicine Xiamen361015China Fujian Science and Technology Innovation Laboratory for Optoelectronic Information of China Fuzhou350108China

In recent years,persistent luminescence materials(PLMs)excited by X-rays and emitting in biological windows have received extensive attention in the field of high-sensitivity *** metal Mn^(2+)is an ideal emission center,but few studies focus on Mn^(2+)-doped PLMs with X-ray excitation and biological window ***,we report a Mn^(2+)-doped PLM,liYGeO_(4):Mn^(2+)(LYGM),with excellent biological window persistent luminescence *** excitation by UV,LYGM produces a durable biological window of persistent luminescence emission at 660 nm for up to 20 *** *** can be repeatedly excited by X-rays,resulting in long-term biological window persistent luminescence *** addition,we obtain LYGM around 200 nm in diameter by ball milling and centrifugation and improve its biocompatibility by surface modification to apply it to in vivo imaging in *** LYGM are injected into mice through the tail vein,in situ excitation of X-rays can be *** the persistent luminescence decays,LYGM can be re-excited for repeated ***,LYGM shows potential prospects for in vivo deep tissue and long-term bioimaging.

关键词： Persistent luminescence X-ray Biological window In vivo imaging Rare earths