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Celecoxib attenuates 5-fluorouracil-induced apoptosis in HCT-15 and HT-29 human colon cancer cells

World Journal of Gastroenterology 2007年第13期13卷 1947-1952页

作者： yun Jeong lim Jong Chul Rhee Young Mee Bae Wan Joo Chun Department of Internal Medicine Dongguk University International Hospital Dongguk University College of Medicine Goyang Korea Division of Gastroenterology Department of Internal Medicine Samsung Medical Center Sungkyunkwan University School of Medicine Seoul Korea Department of Parasitology Seoul National University College of Medicine Seoul Korea Department of Pharmacology Kangwon National University College of Medicine Chuncheon Korea

AIM： To investigate the combined chemotherapeutic effects of celecoxib when used with 5-FU in vitro. METHODS： Two human colon cancer cell lines （HCT-15 and HT-29） were treated with 5-FU and celecoxib, alone and in combination. The effects of each drug were evaluated using the MTT （3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyltetrazolium bromide） assay, flow cytometry, and western blotting. RESULTS： 5-FU and celecoxib showed a dosedependent cytotoxic effect. When treated with 10-3 mol/L 5-FU （IC50） and celecoxib with its concentration ranging from 10.8 mol/L to 10.4 mol/L of celecoxib, cells showed reduced cytotoxic effect than 5-FU （10.3 mol/L） alone. Flow cytometry showed that celecoxib attenuated 5-FU induced accumulation of cells at subG1 phase. Western blot analyses for caspase-3 and poly （ADP-ribose） polymerase （PARP） cleavage showed that celecoxib attenuated 5-FU induced apoptosis. Western blot analyses for cell cycle molecules showed that G2/M arrest might be possible cause of 5-FU induced apoptosis and celecoxib attenuated 5-FU induced apoptosis via blocking of cell cycle progression to the G2/M phase, causing an accumulation of cells at the GI/S phase. CONCLUSION： We found that celecoxib attenuated cytotoxic effect of 5-FU. Celecoxib might act via inhibition of cell cycle progression, thus preventing apoptosis induced by 5-FU.

关键词： Antagonism Celecoxib Colorectal neoplasms Fluorouracil

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Risk factors for metachronous gastric carcinoma development after endoscopic resection of gastric dysplasia: Retrospective, single-center study

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World Journal of Gastroenterology 2017年第24期23卷 4407-4415页

作者： hee Seok Moon Gee Young yun Ju Seok Kim Hyuk Soo Eun Sun Hyung Kang Jae Kyu Sung Hyun Yong Jeong Kyu-Sang Song Department of Internal Medicine Chungnam National University Hospital Chungnam National University School of Medicine Department of Pathology Chungnam National University Hospital Chungnam National University School of Medicine

To determine the gastric adenocarcinoma (GAC) occurrence rate and related factors, we evaluated the follow-up results of patients confirmed to have gastric dysplasia after endoscopic resection (ER). METHODSWe retrospectively analyzed the medical records, endoscopic examination records, endoscopic procedure records, and histological records of 667 cases from 641 patients who were followed-up for at least 12 mo, from among 1273 patients who were conformed to have gastric dysplasia after Endoscopic mucosal resection (EMR) or endoscopic submucosal dissection (ESD) of gastric mucosal lesions between January 2007 and August 2013 at the Chungnam National University Hospital. RESULTSThe mean follow-up period was 33.8 mo, and the median follow-up period was 29 mo (range: 12-87). During the follow-up period, the occurrence of metachronous GAC was 4.0% (27/667). The mean and median interval periods between the occurrence of metachronous GAC and endoscopic treatment of gastric dysplasia were 36.3 and 34 mo, respectively (range: 16-71). The factors related to metachronous GAC occurrence after ER for gastric dysplasia were male sex (5.3% vs 1.0%), open-type atrophic gastritis (9.5% vs 3.4%), intestinal metaplasia (6.8% vs 2.4%), and high-grade dysplasia (HGD; 8.4% vs 3.2%). Among them, male sex [OR: 5.05 (1.18-21.68), P = 0.029], intestinal metaplasia [OR: 2.78 (1.24-6.23), P = 0.013], and HGD [OR: 2.70 (1.16-6.26), P = 0.021] were independent related factors in multivariate analysis. Furthermore, 24 of 27 GAC cases (88.9%) occurred at sites other than the previous resection sites, and 3 (11.1%) occurred at the same site as the previous resection site. CONCLUSIONMale sex, intestinal metaplasia, and HGD were significantly related to the occurrence of metachronous GAC after ER of gastric dysplasia, and most GACs occurred at sites other than the previous resection sites.

关键词： Gastric dysplasia Neoplasms Second primary Endoscopic mucosal resection Stomach neoplasms

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Measles outbreaks in the Kyeongin area of the Republic of Korea,2013-2014:A single-center experience in a country of measles elimination

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Asian Pacific Journal of Tropical Medicine 2017年第1期10卷 67-72页

作者： Sun Hyoung Park Dong Hun Lee Jang Yong Jin Young-lim Shin Meeyong Shin Sung Shin Kim Won Suk Suh Jae Ock Park Yong hee Hong Department of Pediatrics College of MedicineSoonchunhyang UniversityBucheon HospitalBucheonRepublic of Korea

Objective: To identify the source of infection and determine the clinical features and laboratory finding of measles infection,Methods: In 27 measles patients,except for 3 adult patients,the rest of 24 pediatric measles cases were analyzed with regard to age,sex,immunization status,transmission routes and molecular genotyping of measles virus,Eighteen measles patients who admitted in isolation ward were set apart to investigate clinical findings and its correlation with laboratory characteristics,Retrospective analysis of cases was conducted in this study,Results: Of the 24 pediatric patients,23(95.8%) had not received any measles-containing vaccine(MCV),Sixteen of the patients(66.7%) were aged <12 months,The suspicious index case of a girl aged 34 months was not vaccinated with MCV1 and got measles after a trip to Philippines,and molecular genotype was revealed as B3,Measles outbreaks in the community such as a restaurant were followed by this one imported case,According to analysis of 18 patients admitted in isolation ward,the median level of C-reactive protein(CRP) was 0.38 mg/d L and that of lactate dehydrogenase(LDH) was 1 200 IU/L,All of the 18 patients had LDH levels above the normal range,Age correlated with CRP(ρ = 0.528,P = 0.024) and LDH(ρ = 0.501,P = 0.034),The duration of fever was correlated with the duration of fever before rash(ρ = 0.898,P < 0.01),The duration of hospitalization was correlated with CRP(ρ = 0.586,P = 0.011),The white blood cell counts were correlated with the levels of LDH(ρ = 0.505,P = 0.033),aspartate aminotransferase(ρ = 0.507,P = 0.032),and alanine aminotransferase(ρ = 0.481,P = 0.043),Conclusions: Early weaning of maternally derived measles antibodies therefore vaccination of MCV1 at a young age from 9 months to 12 months should be considered in situations of early exposure,Furthermore there is a call for consideration of scheduling an earlier age for the first dose of MMR vaccine in Europe,It is necessary for Korea to investigat

关键词： Measles Disease outbreaks Pediatrics

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LRP<sub>5</sub>Affects Homeostasis of the Periodontal Complex

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Open Journal of Stomatology 2020年第3期10卷 29-37页

作者： Joo-Hyung Kim Ye-Hyun Kim Won hee lim Department of Orthodontics School of Dentistry & Dental Research Institute Seoul National University Seoul Korea

Purpose: The signals responsible for homeostasis in the periodontal complex are unclear. The purpose of this study was to evaluate the role of Low-density lipoprotein receptor-related protein 5(LRP5) in this process by removing LRP5, and observing the effects of LRP5 depletion on cells of the periodontal structures. Material and Methods: The function of this LRP5 was evaluated by conditional elimination of the LRP5 gene using an Osteocalcin Cre driver. The OCN-Cre;? mice were examined using micro-CT and histology, immunohistochemistry to evaluate the periodontal complex. Results: Elimination of LRP5 in the periodontal complex of OCN-Cre;??mice results in a different expression of Fibromodulin in the periodontal ligament space. A decrease in osteoclastic activity was found in the periodontal ligament. Conclusion: Osteoclastic activities are decreased and expression of fibromodulin is decreased, which implies the involvement of LRP5 in homeostasis of the periodontal ligament.

关键词： LRP5 Periodontal Ligament Homeostasis

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Simultaneous multiple coronary arteries thrombosis in patients with STEMI

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Journal of Geriatric Cardiology 2018年第3期15卷 241-243页

作者： Seunghwan Kim Sang-Hoon Seol Dong-hee Park yun-Seok Song Dong-Kie Kim Ki-Hun Kim Doo-Il Kim Pil-Sang Song Department of Internal Medicine Haeundae Paik Hospital lnje University college of Medicine Busan Republic of Korea Department of Internal Medicine Se-Jong General Hospital Incheon Republic of Korea

Simultaneous thrombosis affecting more than one coronary artery has been reported to occur in about 4.8% of the cases at the time primary percutaneous coronary intervention （PCI）. Simultaneous multiple coronary arteries throm-bosis is uncommon and can lead to a fatal outcome. Careful attention should be given to identification of abnormal ECG and coronary angiography （CAG） results. The affected ves- sel should be opened timely and efficiently in an effort to save the myocardium and reduce serious complications such as congestive heart failure, ventricular arrhythmia, cardiogenic shock, or sudden cardiac death.

关键词： Multiple Simultaneous STEMI Thrombosis

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基于UPLC-MS/MS的全氟及多氟烷基化合物在斑马鱼体内的生物富集与组织分布研究

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分析测试学报 2024年第8期43卷 1189-1196页

作者：曾海深何财杨运云曾细柳谢嘉良刘宁邓洁薇栾天罡化学与精细化工广东省实验室揭阳分中心(广东省榕江实验室) 广东揭阳515200 广东工业大学生物医药学院广东广州510006 广东省科学院测试分析研究所(中国广州分析测试中心) 广东广州510070 五邑大学环境与化学工程学院广东江门529020

采用超高效液相色谱-串联质谱(UPLC-MS/MS)研究了全氟及多氟烷基化合物(PFASs)在斑马鱼体内的生物富集与组织分布。以全氟辛烷磺酸(PFOS)、全氟辛酸(PFOA)、全氟己烷磺酸(PFHxS)和全氟癸酸(PFDA)对斑马鱼联合暴露7、21、28 d,暴露浓度为... 详细信息

采用超高效液相色谱-串联质谱(UPLC-MS/MS)研究了全氟及多氟烷基化合物(PFASs)在斑马鱼体内的生物富集与组织分布。以全氟辛烷磺酸(PFOS)、全氟辛酸(PFOA)、全氟己烷磺酸(PFHxS)和全氟癸酸(PFDA)对斑马鱼联合暴露7、21、28 d,暴露浓度为100μg/L。斑马鱼不同组织器官分离后,用甲醇进行提取,以UPLC-MS/MS进行检测,负离子多反应监测(MRM)模式进行定量测定。结果表明,4种PFASs在1~200μg/L范围内线性关系良好,相关系数(r)不小于0.9974,检出限(LOD)和定量下限(LOQ)分别为0.072~0.152 ng/g和0.287~0.609 ng/g。斑马鱼暴露于4种PFASs后,其体内各器官均分布有上述4种污染物。其中PFDA和PFOS在暴露7、21、28 d的斑马鱼体内器官的积累程度相对较高;PFOA主要在肠道和肝脏中的积累量较高;PFHxS在斑马鱼体内的积累量最低,在各器官中的含量对4种污染物含量总和的相对贡献均低于13%。该研究为探索PFASs在水生生物体内的环境行为和生态风险提供了科学依据。

关键词：超高效液相色谱-串联质谱斑马鱼全氟及多氟烷基化合物生物富集组织分布

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Usefulness of colonic tattooing using indocyanine green in patients with colorectal tumors

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World Journal of Clinical Cases 2018年第13期6卷 632-640页

作者： Jae Ho Park hee Seok Moon In Sun Kwon Gee Young yun Seo hee Lee Dae Hwa Park Ju Seok Kim Sun Hyung Kang Eaum Seok Lee Seok Hyun Kim Jae Kyu Sung Byung Seok Lee Hyun Yong Jeong Division of Gastroenterology Department of Internal Medicine Chungnam National University Hospital Chungnam National University college of Medicine Clinical Trials Center Chungnam National University Hospital

AIM To prove that tattooing using indocyanine green(ICG) is feasible in laparoscopic surgery for a colon *** From January 2012 to December 2016, all patients who underwent laparoscopic colonic surgery were retrospectively screened, and 1010 patients with colorectal neoplasms were included. Their lesions were tattooed with ICG the day before the operation. The tattooed group(TG) included 114 patients, and the non-tattooed group(NTG) was selected by propensity score matching of subjects based on age, sex, tumor staging, and operation method(n = 228). In total, 342 patients were enrolled. Between the groups, the changes in [Delta(Δ), preoperative-postoperative] the hemoglobin and albumin levels, operation time, hospital stay, oral ingestion period, transfusion, and perioperative complications were compared. RESULTS Preoperative TG had a shorter operation time(174.76 ± 51.6 min vs 192.63 ± 59.9 min, P < 0.01), hospital stay(9.55 ± 3.36 d vs 11.42 ± 8.23 d, P < 0.01), and postoperative oral ingestion period(1.58 ± 0.96 d vs 2.81 ± 1.90 d, P < 0.01). The Δ hemoglobin(0.78 ± 0.76 g/dL vs 2.2 ± 1.18 g/dL, P < 0.01) and Δ albumin(0.41 ± 0.44 g/dL vs 1.08 ± 0.39 g/dL, P < 0.01) levels were lower in the TG. On comparison of patients in the "N0" and "N1 or N2" groups, the N0 colon cancer group had a better operation time, length of hospital stay, oral ingestion period, Δ hemoglobin, and Δ albumin results than those of the N1 or N2 group. The operation methods affected the results, and laparoscopic anterior resection(LAR) showed similar results. However, for left and right hemicolectomy, both groups showed no difference in operation time or hospital *** Preoperative tattooing with ICG is useful for laparoscopic colectomy, especially in the N0 colon cancer group and LAR.

关键词： Colorectal neoplasm Laparoscopic surgery Endoscopic tattoo Indocyanine green Perioperative

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Development of novel gene carrier using modified nano hydroxyapatite derived from equine bone for osteogenic differentiation of dental pulp stem cells

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Bioactive Materials 2021年第9期6卷 2742-2751页

作者： Myung Chul Lee Hoon Seonwoo Kyoung Je Jang Shambhavi Pandey Jaewoon lim Sangbae Park Jae Eun Kim yun-Hoon Choung Pankaj Garg Jong Hoon Chung Department of Biosystems&Biomaterials Science and Engineering Seoul National UniversitySeoul08826Republic of Korea Department of Biosystems Engineering Seoul National UniversitySeoul08826Republic of Korea Department of Industrial Machinery Engineering Sunchon National University315 Maegok-dongSunchon57922Republic of Korea Interdisciplinary Program in IT-Bio Convergence System Sunchon National UniversitySuncheon57922Republic of Korea Division of Agro-system Engineering Gyeongsang National University501 Jinju-daeroJinju52828Republic of Korea Institute of Agriculture&Life Science Gyeongsang National UniversityJinju52828Republic of Korea Research Institute for Agriculture and Life Sciences Seoul National UniversitySeoul08826Republic of Korea Department of Otolaryngology Ajou University School of MedicineSuwon16499Republic of Korea Ajou University Graduate School of Medicine Bk21 Plus Research Center for Biomedical SciencesSuwon16499Republic of Korea BK21 Global Smart Farm Educational Research Center Seoul National UniversitySeoul08826Republic of Korea

Hydroxyapatite(HA)is a representative substance that induces bone *** research team extracted nanohydroxyapatite(EH)from natural resources,especially equine bones,and developed it as a molecular biological ***(PEI)was used to coat the EH to develop a gene *** verify that PEI is well coated in the EH,we first observed the morphology and dispersity of PEI-coated EH(pEH)by electron *** pEH particles were well distributed,while only the EH particles were not distributed and ***,the existence of nitrogen elements of PEI on the surface of the pEH was confirmed by EDS,calcium concentration measurement and fourier transform infrared spectroscopy(FT-IR).Additionally,the pEH was confirmed to have a more positive charge than the 25 kD PEI by comparing the zeta *** a result of pGL3 transfection,pEH was better able to transport genes to cells than 25 kD *** verification as a gene carrier for pEH,we induced osteogenic differentiation of DPSCs by loading the BMP-2 gene in pEH(BMP-2/pEH)and delivering it to the *** a result,it was confirmed that osteogenic differentiation was promoted by showing that the expression of osteopontin(OPN),osteocalcin(OCN),and runt-related transcription factor 2(RUNX2)was significantly increased in the group treated with BMP-2/*** conclusion,we have not only developed a novel nonviral gene carrier that is better performing and less toxic than 25 kD PEI by modifying natural HA(the agricultural byproduct)but also proved that bone differentiation can be effectively promoted by delivering BMP-2 with pEH to stem cells.

关键词： Nanocomposites Biomedical applications Hydroxyapatite Polyethyleneimine Gene carrier Osteogenic differentiation

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Actinomycosis of the appendix mimicking appendiceal tumor:A case report

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World Journal of Gastroenterology 2010年第3期16卷 395-397页

作者： Sang-yun Lee hee-Jin Kwon Jin-Han Cho Jong-Young Oh Kyung-Jin Nam Jin-Hwa Lee Seong-Kuk Yoon Myong-Jin Kang Jin-Sook Jeong Department of Radiology Dong-A University Hospital Department of Pathology Dong-A University Hospital

Actinomycosis is an uncommon chronic infectious disease. Common sites of involvement include the cervicofacial, thoracic and abdominopelvic regions. In abdominopelvic actinomycosis, the ileocecal region, including the appendix, is the most commonly involved site. In some reports, limited appendiceal actinomycosis has revealed a thickened appendiceal wall with peri-appendiceal inflammation as acute appendicitis or perforated appendicitis. We experienced pathologically confirmed intraluminal limited appendiceal actinomycosis without peri-appendiceal infiltration. Here, we report the computed tomography and ultrasound findings.

关键词： Actinomyces Actinomycosis Appendiceal neoplasms Appendicitis

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Identification of Aspergillus nidulans Genes Regulated by the Transcription Factor, NsdD

Identification of Aspergillus nidulans Genes Regulated by th...

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The 7th Korea-China Joint Symposium for Mycology

作者： Suk-Yi WOO yun-hee PARK hee-Moon PARK Department of Microbiology School of Biosciences and Biotechnology Chungnam National University Department of Microbiology School of Biosciences and Biotechnology Chungnam National University Department of Microbiology School of Biosciences and Biotechnology Chungnam National University

＜正＞Sexual differentiation of A. nidulans is regulated by NsdD （never in sexual development）, which encodes a GATA-type transcription factor with the type IVb zinc finger DNA-binding domain. Although expression of...

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