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Induction of clusterin Expression by Neuronal Cell Death in Zebrafish

Journal of Genetics and Genomics 2014年第11期41卷 583-589页

作者： yun-Mi Jeong Tae-Eun Jin Jung-Hwa Choi Mi-Sun Lee Hyun-Taek Kim Kyu-Seok Hwang Doo-Sang Park Hyun-Woo Oh Joong-Kook Choi Vladimir Korzh Melitta Schachner Kwan-hee You Cheol-hee Kim Department of Biology Chungnam National University Korea Research Institute of Bioscience and Biotechnology Department of Biochemistry College of MedicineChungbuk National University Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research Keck Center for Collaborative Neuroscience and Department of Cell Biology and Neuroscience Rutgers University Center for Neuroscience Shantou University Medical College

Clusterin, a protein associated with multiple functions, is expressed in a wide variety of mammalian tissues. Although clusterin is known to be involved in neurodegenerative diseases, ageing, and tumorigenesis, a detailed analysis of the consequences of gain- or loss-of- function approaches has yet to be performed to understand the underlying mechanisms of clusterin functions. Since clusterin levels change in neurological diseases, it is likely that clusterin contributes to cell death and degeneration in general. Zebrafish was investigated as a model system to study human diseases. During development, zebrafish clusterin was expressed in the notochord and nervous system. Embryonic overexpression of clusterin by mRNA microinjection did not affect axis formation, whereas its knock-down by anti-sense morpholino treatment resulted in neuronal cell death. To analyze the function of clusterin in neurodegeneration, a transgenic zebrafish was investigated, in which nitroreductase expression is regulated under the control of a neuron-specifc huC promoter which is active between the stages of early neuronal precursors and mature neurons. Nitroreductase turns metronidazole into a cytotoxic agent that induces cell death within 12 h. After metronidazole treatment, transgenic zebrafish showed neuron-specific cell death. Interestingly, we also observed a dramatic induction of clusterin expression in the brain and spinal cord in these fish, suggesting a direct or indirect role of clusterin in neuronal cell death and thus, more generally, in neurodegeneration.

关键词： Clusterin Neuronal cell death Neurodegeneration Zebrafish

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In vivo biodistribution of topical low molecular weight heparin-taurocholate in a neovascularized mouse cornea

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International Journal of Ophthalmology(English edition) 2018年第9期11卷 1435-1439页

作者： Chan hee Moon Ji yun Lee Eun Soon Kim Jin Hyoung Park Sang-Yeob Kim Jae Yong Kim Hungwon Tchah Department of Ophthalmology Asan Medical CenterUniversity of Ulsan College of Medicine Research Institute for Biomacromolecules University of Ulsan College of MedicineAsan Medical Center Department of Convergence Medicine University of Ulsan College of MedicineAsan Medical Center

AIM： To investigate the ocular biodistribution and clearance of topically administered 7-taurocholic acid conjugated low-molecular weight heparin（LHT7） in a neovascularized mouse cornea using an in vivo optical imaging system. METHODS： A total of 10 eyes of 6 to 8-week-old BALB/c mice were analyzed. Corneal neovascularization（CoNV） was induced in the inferior cornea（IC） of each animal by penetrating the stroma with two interrupted sutures. The development of CoNV was verified after one week and the area of each neovascularized region was measured. A near-infrared fluorescent probe of 20 μmol/L Cy5.5 labeled LHT7（LHT7-Cy5.5） in 0.02 mL solution was topically instilled onto the cornea in the experimental group（n=5）. Free-Cy5.5 of 20 μmol/L in 0.02 mL was instilled in the control group（n=5）. In vivo optical images were obtained before instillation and 5 min, 2, 4, and 6 h after instillation. The intensities were separately measured at the superior cornea（SC） and the IC. RESULTS： The mean CoNV areas were 1.97±0.17 mm^2 and 1.92±0.96 mm^2 in the experimental and control groups, respectively（P=0.832）. The SC remained normal in all 10 subject animals. The IC intensity of the LHT7-Cy5.5 was greater than the SC intensity at 5 min（P=0.038）, 2 h（P=0.041）, and 4 h（P=0.041） after application. The IC intensity fell to less than half of its initial value（42.9%±8.6%） at 6 h in the experimental group. In the control mice, here were no significant differences in the free-Cy5.5 intensity between the IC and SC. CONCLUSION： Topically administered LHT7 shows a high biodistribution in CoNV areas for 4 h and should be reapplied accordingly to maintain its effects. In vivo optical imaging can be a useful tool for evaluating the ocular biodistribution of a drug in an animal model.

关键词： comeal neovascularization in vivo optical imaging low-molecular weight heparin ocular biodistribution

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Solvent effects on the Asymmetric Pauson-Kahnd-type Reaction by Rhodium

Solvent effects on the Asymmetric Pauson-Kahnd-type Reaction...

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13th Asian Chemical Congress

作者： yun-hee CHOI Department of Chemistry College of ScienceKorea University

＜正＞Since the first report on the rhodium catalyzed enantioselective PKR under a CO atmosphere in the presence of the atropisomeric ligand BINAP.l Systematic efforts to perfect this protocol in terms of the chemical yield and enantioselectivity have since been *** various factors,the effect of CO pressure,tethers,and ligands on the reaction efficiency and enantioselectivity were extensively examined.2,3 Further optimization was tried by elucidation of role of the solvent during *** this study,

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Neural Tract Degeneration Correlates with Functional Impairment in Patients with Anoxic Brain Injury:A Tract-based Spatial Statistics Study

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Neuroscience Bulletin 2020年第8期36卷 932-936页

作者： Ji-yun Park Sung Ho Park Jeong-hee Yang Han Do Lee Jiho Lee Ki-Hyun Byeon Seo Young Park Dongseok Yang Department of Neurology University of Ulsan College of MedicineUlsan University HospitalUlsan 44033Korea Department of Neurosurgery Ulsan University HospitalUlsan 44033Korea Department of Plastic Surgery Biomedical Research InstituteSeoul National University HospitalSeoul 03080Korea Department of Physical Therapy College of UlsanUlsan 44022Korea Department of Occupational and Environmental Medicine University of Ulsan College of MedicineUlsan University HospitalUlsan 44033Korea Physical Medicine and Rehabilitation University of Ulsan College of MedicineUlsan University HospitalUlsan 44033Korea Department of Clinical Epidemiology and Biostatistics Asan Medical CenterSeoul 05505Korea

Dear Editor,Anoxic brain injury(ABI)is a devastating event caused by cardiac arrest,chemical exposure,or *** recent advances in medical diagnostic techniques and treatments,the survival rate after ABI is still very *** studies have reported that 52.6%-64.0%of people who received cardiopulmonary resuscitation after nontraumatic cardiac arrest die,and 9.0%-18.6%remain in a permanent vegetative state within 28 days of treatment[1,2].

关键词： Tract cardiac treatment

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Pleomorphic Adenoma with Exuberant Squamous Metaplasia and Keratin Cysts Mimicking Squamous Cell Carcinoma in Minor Salivary Gland

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Open Journal of Pathology 2013年第3期3卷 113-116页

作者： Sharon lim Inju Cho Jun-hee Park Sung-Chul lim Department of Pathology Chosun University College of Medicine Gwangju South Korea

Salivary gland tumors, the second most common neoplasm of the mouth after squamous cell carcinoma, account for a significant proportion of tumors of the oral and perioral regions. An unusual case of adenoma presented as a solitary intraoral palatine mass in a 32-year-old woman is reported here. The tumor was interpreted as an unusual pleomorphic adenoma because of the presence of exuberant squamous metaplasia, clinically mimicking squamous cell carcinoma. Moreover, the presence of cystic structures filled with keratinized material was also salient feature. Pleomorphic adenomas may occasionally display focal squamous metaplastic changes;when extensive, it presents the potential for misinterpretation of the histology as indicative of well-differentiated squamous cell carcinoma.

关键词： Pleomorphic Adenoma Squamous Metaplasia Keratin Cyst Squamous Cell Carcinoma Salivary Gland

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Usefulness of the acromioclavicular joint cross-sectional area as a diagnostic image parameter of acromioclavicular osteoarthritis

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World Journal of Clinical Cases 2022年第7期10卷 2087-2094页

作者： Young Joo Jee Youn Moon Jung Youn Han yun-Sic Bang Keum Nae Kang Young Su lim Young-SoonChoi Young-Uk Kim Department of Anesthesiology and Pain Medicine CHA Ilsan Medical CenterSchool of MedicineCHA UniversityIlsanGyeonggi-do 10414South Korea Department of Anesthesiology and Pain Medicine Seoul National University Hospital College of MedicineSeoul 03080South Korea Department of Anesthesiology and Pain Medicine CHA Bundang Medical CenterSchool of MedicineSeongnamGyeonggi-do 13496South Korea Department of Anesthesiology and Pain Medicine National Police HospitalSeoul 05715South Korea Department of Anesthesiology and Pain Medicine Catholic Kwandong UniversityCollege of MedicineInternational St.Mary’s HospitalIncheon 22711South Korea

BACKGROUND Acromioclavicular joint(ACJ)space narrowing has been considered to be an important diagnostic image parameter of ACJ osteoarthritis(ACJO).However,the morphology of the ACJ space is irregular because of osteophyte formation,subchondral irregularity,capsular distention,sclerosis,and ***,we created the ACJ cross-sectional area(ACJCSA)as a new diagnostic image parameter to assess the irregular morphologic changes of the *** To hypothesize that the ACJCSA is a new diagnostic image parameter for *** ACJ samples were obtained from 35 patients with ACJO and 30 healthy individuals who underwent shoulder magnetic resonance(S-MR)imaging that revealed no evidence of *** coronal,T2-weighted,fat-suppressed SMR images were acquired at the ACJ level from the two *** measured the ACJCSA and the ACJ space width(ACJSW)at the ACJ on the S-MR images using our imaging analysis *** ACJCSA was measured as the cross-sectional area of the *** ACJSW was measured as the narrowest point between the acromion and the *** The average ACJCSA was 39.88±10.60 mm;in the normal group and 18.80±5.13 mm;in the ACJO *** mean ACJSW was 3.51±0.58 mm in the normal group and 2.02±0.48 mm in the ACJO *** individuals had significantly lower ACJCSA and ACJSW than the healthy *** operating characteristic curve analyses demonstrated that the most suitable ACJCSA cutoff score was 26.14 mm^(2),with 91.4%sensitivity and 90.0%*** The optimal ACJSW cutoff score was 2.37 mm,with 88.6%sensitivity and 96.7%*** though both the ACJCSA and ACJSW were significantly associated with ACJO,the ACJCSA was a more sensitive diagnostic image parameter.

关键词： Acromioclavicular joint Osteoarthritis Cross-sectional area Diagnosis

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Immune enhancement effect of an herb complex extract through the activation of natural killer cells and the regulation of cytokine levels in a cyclophosphamide-induced immunosuppression rat model

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Asian Pacific Journal of Tropical Medicine 2018年第12期11卷 653-658页

作者： Sung Min Woo Woo Rin Choi Dooly Jang Chun Sik Yi Hae lim Kim Kyung Hyeon Kim Jong Tae Kim Won hee Choi Seung hee Jang Min Jeung Kim Ji Hyang Wee Yeon Ki Kim Bao Le Interdisciplinary Program of Biomodulation Myongji UniversityYonginGyeonggi 17058Republic of Korea Center for Nutraceutical and Pharmaceutical Materials Myongji UniversityYonginGyeonggi 17058Republic of Korea Teazen Co. B-606 Acrotower230 Sinim-daeroDongan-guAnyang-siGyeonggi-do 14067Republic of Korea Department of Food Science&Biotechnology Shin Ansan UniversityAnsanGyeonggi 15435Republic of Korea' Division of Bioscience and Bioinformatics Myongji UniversityYonginGyeonggi 17058Republic of Korea Department of Biotechnology Chonnam National UniversityYeosuChonnam 59626Republic of Korea

Objective: To investigate the effects of a herb complex extract(HCE) prepared from Cornus officinalis Sieb. Et Zucc., Eriobotrya japonica Lindley, and olive leaves on immune response of mouse spleen NK cells in vitro and in vivo analysis. Methods: The activity of natural killer(NK) cells was measured in splenocytes and YAC-1 cells. Mice were immunosuppressed using cyclophosphamide(5 mg/kg body weight). Three different doses of HCE(200, 400, and 800 mg/kg body weight) and red ginseng extract(800 mg/kg body weight) which was used as standard immunomodulatory herb were administered orally for 4 weeks. The body weight, dietary, water intake, organs(liver, thymus, and spleen) weight, completed blood count, and cytokines(tumor necrosis factor alpha, interferon gamma, and interleukin-2) production was measured. Results: At the maximum concentration of HCE, the activity of NK cells was increased by 48.5%. HCE increased liver, spleen, and thymus weights without altering numbers of white blood cells, lymphocytes, and neutrophils in a cyclophosphamide-induced immunosuppression rat model. However, HCE recovered the inhibited cytokine expression; HCE(800 mg/kg) increased cytokines levels. The results indicate the immune enhancement potential of this HCE. Conclusion: The HCE enhances immunity by increasing NK cell activity, regulating cytokine levels, and maintaining spleen weight. Therefore, it may be used as a potential immunity enhancer.

关键词： Immune system enhancement Cyclophosphamide Cornus officinalis Sieb Et Zucc Eriobotrya japonica Lindley Olive leaves

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Development of a rapid test kit for SARS-CoV-2:an example of product design

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Bio-Design and Manufacturing 2020年第2期3卷 83-86页

作者： Zhanfeng Cui Hong Chang Hui Wang Boon lim Chia-Chen Hsu Yejiong Yu Huidong Jia yun Wang Yida Zeng Mengmeng Ji Weizhi Liu Catriona Inverarity Wei E.Huang Oxford Suzhou Centre for Advanced Research(OSCAR) University of OxfordSuzhouJiangsuPeople's Republic of China Department of Engineering Science University of OxfordParks RoadOxford OX13PJUK Department of Engineering Science Institute of Biomedical EngineeringUniversity of OxfordOxford OX37DQUK

We present an example of applying'need-driven'product design principle to the development of a rapid test kit to detect SARS-COV-2(COVID-19).The tests are intended for use in the field and,longer term,for home *** detect whether a subject is currently infected with the virus and is *** urgent need for large numbers of tests in field setting imposes constraints such as short test time and lack of access to specialist equipment,laboratories and skilled technicians to perform the test and interpret *** meet these needs,an antigen test based on RT-LAMP with colorimetric readout was *** use of swab sample with no RNA extraction was *** extensive experimental study(reported elsewhere),a rapid test kit has been fabricated to satisfy all design criteria.

关键词： COVID-19 Antigen detection Rapid test Product design SAR-Cov-2 RT-LAMP

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Recrystallization behavior of cold rolled Al-Zn-Mg-Cu fabricated by twin roll casting

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Transactions of Nonferrous Metals Society of China 2014年第7期24卷 2226-2231页

作者： yun-Soo LEE Won-Kyoung KIM Dong-A JO Cha-Yong lim Hyoung-Wook KIM Light Metals Division Korea Institute of Materials Science

The Al-Zn-Mg-Cu alloy strip can be successfully fabricated by twin roll casting. In order to determine the effect of thickness reduction and heat treatment temperature on the recrystallization behavior, the strips were rolled and subsequently heat treated under various conditions. As a result, a fine grained microstructure （average grain size -13 μm, average grain aspect ratio -1.7） and high mechanical properties （UTS ≥360 MPa, δ ≥20%） were obtained by cold rolling with thickness reduction of 60% and heat treatment at 500 ℃ for 1 h. Also, the effect of the microstructure on mechanical properties of the Al-Zn-Mg-Cu alloy strip was discussed. Consequently, the low cost and high strength Al-Zn-Mg-Cu alloy sheet can be fabricated by twin roll casting by proper thermo-mechanical processes.

关键词： Al-Zn-Mg-Cu twin roll casting cold rolling recrystallization

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All-in-one glycol chitosan nanoparticles for co-delivery of doxorubicin and anti-PD-L1 peptide in cancer immunotherapy

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Bioactive Materials 2023年第10期28卷 358-375页

作者： Sukyung Song Man Kyu Shim Suah Yang Jaewan Lee Wan Su yun Hanhee Cho Yujeong Moon Jin Young Min Eun hee Han Hong Yeol Yoon Kwangmeyung Kim College of Pharmacy Graduate School of Pharmaceutical SciencesEwha Womans UniversitySeoul03760Republic of Korea Medicinal Materials Research Center Biomedical Research DivisionKorea Institute of Science and Technology(KIST)Seoul02792Republic of Korea KU-KIST Graduate School of Converging Science and Technology Korea UniversitySeoul02841Republic of Korea Department of Bioengineering Korea UniversitySeoul02841Republic of Korea Research Center for Bioconvergence Analysis Korea Basic Science Institute(KBSI)Cheongju28119Republic of Korea Department of Toxicology College of PharmacyChungnam National UniversityDaejeon34134Republic of Korea

Synergistic immunotherapy of immune checkpoint blockade (ICB) and immunogenic cell death (ICD) has shown remarkable therapeutic efficacy in various cancers. However, patients show low response rates and undesirable outcomes to these combination therapies owing to the recycling mechanism of programmed death-ligand 1 (PD-L1) and the systemic toxicity of ICD-inducing chemotherapeutic drugs. Herein, we propose all-in-one glycol chitosan nanoparticles (CNPs) that can deliver anti-PD-L1 peptide (PP) and doxorubicin (DOX) to targeted tumor tissues for a safe and more effective synergistic immunotherapy. The PP-CNPs, which are prepared by conjugating ᴅ-form PP (NYSKPTDRQYHF) to CNPs, form stable nanoparticles that promote multivalent binding with PD-L1 proteins on the targeted tumor cell surface, resulting in effective lysosomal PD-L1 degradation in contrast with anti-PD-L1 antibody, which induces recycling of endocytosed PD-L1. Consequently, PP-CNPs prevent subcellular PD-L1 recycling and eventually destruct immune escape mechanism in CT26 colon tumor-bearing mice. Moreover, the ICD inducer, DOX is loaded into PP-CNPs (DOX-PP-CNPs) for synergistic ICD and ICB therapy, inducing a large number of damage-associated molecular patterns (DAMPs) in targeted tumor tissues with minimal toxicity in normal tissues. When the DOX-PP-CNPs are intravenously injected into CT26 colon tumor-bearing mice, PP and DOX are efficiently delivered to the tumor tissues via nanoparticle-derived passive and active targeting, which eventually induce both lysosomal PD-L1 degradation and substantial ICD, resulting in a high rate of complete tumor regression (CR: 60%) by a strong antitumor immune response. Collectively, this study demonstrates the superior efficacy of synergistic immunotherapy using all-in-one nanoparticles to deliver PP and DOX to targeted tumor tissues.

关键词： Chitosan nanoparticle Synergistic immunotherapy Immune checkpoint blockade Multivalent binding Immunogenic cell death

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