Immune enhancement effect of an herb complex extract through the activation of natural killer cells and the regulation of cytokine levels in a cyclophosphamide-induced immunosuppression rat model

作     者：Sung Min Woo Woo Rin Choi Dooly Jang Chun Sik Yi Hae Lim Kim Kyung Hyeon Kim Jong Tae Kim Won Hee Choi Seung Hee Jang Min Jeung Kim Ji Hyang Wee Yeon Ki Kim Bao Le 

作者机构：Interdisciplinary Program of BiomodulationMyongji UniversityYonginGyeonggi 17058Republic of Korea Center for Nutraceutical and Pharmaceutical MaterialsMyongji UniversityYonginGyeonggi 17058Republic of Korea Teazen Co.B-606 Acrotower230 Sinim-daeroDongan-guAnyang-siGyeonggi-do 14067Republic of Korea Department of Food Science&BiotechnologyShin Ansan UniversityAnsanGyeonggi 15435Republic of Korea' Division of Bioscience and BioinformaticsMyongji UniversityYonginGyeonggi 17058Republic of Korea Department of BiotechnologyChonnam National UniversityYeosuChonnam 59626Republic of Korea 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2018年第11卷第12期

页      面：653-658页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 10[医学] 100602[医学-中西医结合临床] 

基　　金：Cooperative Research Program for Agriculture Science and Technology Development(Project No.PJ01321501) Rural Development Administration Republic of Korea 

主　　题：Immune system enhancement Cyclophosphamide Cornus officinalis Sieb Et Zucc Eriobotrya japonica Lindley Olive leaves 

摘      要：Objective: To investigate the effects of a herb complex extract(HCE) prepared from Cornus officinalis Sieb. Et Zucc., Eriobotrya japonica Lindley, and olive leaves on immune response of mouse spleen NK cells in vitro and in vivo analysis. Methods: The activity of natural killer(NK) cells was measured in splenocytes and YAC-1 cells. Mice were immunosuppressed using cyclophosphamide(5 mg/kg body weight). Three different doses of HCE(200, 400, and 800 mg/kg body weight) and red ginseng extract(800 mg/kg body weight) which was used as standard immunomodulatory herb were administered orally for 4 weeks. The body weight, dietary, water intake, organs(liver, thymus, and spleen) weight, completed blood count, and cytokines(tumor necrosis factor alpha, interferon gamma, and interleukin-2) production was measured. Results: At the maximum concentration of HCE, the activity of NK cells was increased by 48.5%. HCE increased liver, spleen, and thymus weights without altering numbers of white blood cells, lymphocytes, and neutrophils in a cyclophosphamide-induced immunosuppression rat model. However, HCE recovered the inhibited cytokine expression; HCE(800 mg/kg) increased cytokines levels. The results indicate the immune enhancement potential of this HCE. Conclusion: The HCE enhances immunity by increasing NK cell activity, regulating cytokine levels, and maintaining spleen weight. Therefore, it may be used as a potential immunity enhancer.