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Intestinal ischemia-reperfusion of macaques triggers a strong innate immune response

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World Journal of Gastroenterology 2014年第41期20卷 15327-15334页

作者： Hao Wu Yi-yun deng Ling Liu Qing-Hua Tan Chun-Hui Wang Mei-Mei Guo Yong-Mei Xie Cheng-Wei Tang Department of Gastroenterology West China HospitalSichuan University Department of Intensive Care Unit West China HospitalSichuan University Laboratory Peptides Related to Human Diseases West China Hospital Sichuan University

AIM: To investigate inflammatory injury in the intestinal mucosa after intestinal ischemia-reperfusion (IIR) with Toll-like receptor (TLR)-mediated innate immunity. METHODS: Ten macaques were randomized into control and IIR groups. The distribution and expression level of TLR2, TLR4, MD2, nuclear factor (NF)-kappa B p65 and interferon (IFN)-gamma were measured by immunohistochemical stain and western blotting. The mRNA expression of TLR4, TLR2, MD2, interleukin (IL)-1 beta and tumor necrosis factor (TNF)-alpha were measured by reverse transcriptase-polymerase chain reaction. The cytokine levels in blood and intestinal tissues were measured by ELISA. RESULTS: Obvious hemorrhage and erosion of mucosae were seen in the IIR group. Expression of TLR2, TLR4, MD2, NF-kappa B p65 and IFN-gamma. was significantly higher in the IIR group than in the control group (0.13 +/- 0.04, 0.22 +/- 0.04, 0.16 +/- 0.06, 0.65 +/- 0.12, 0.38 +/- 0.10 vs 0.07 +/- 0.04, 0.08 +/- 0.03, 0.04 +/- 0.02, 0.19 +/- 0.06, 0.14 +/- 0.05, P deng Publishing Group Inc. All rights reserved.

关键词： Intestine ischemia reperfusion Toll-like receptors nuclear factor-kappa B Cytokine macaques

Ultrathin Al Oxide Seed Layer for Atomic Layer Deposition of High-κ Al2O3 Dielectrics on Graphene

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Chinese Physics Letters 2020年第7期37卷 80-85页

作者： Hang Yang Wei Chen Ming-Yang Li Feng Xiong Guang Wang Sen Zhang Chu-yun deng Gang Peng Shi-Qiao Qin College of Liberal Arts and Science National University of Defense TechnologyChangsha 410073China China Aerodynamics Research and Development Center Hypervelocity Aerodynamics InstituteMianyang 621000China College of Advanced Interdisciplinary Studies National University of Defense TechnologyChangsha 410073China College of Aerospace Science and Engineering National University of Defense TechnologyChangsha 410073China

Due to the lack of surface dangling bonds in graphene,the direct growth of high-κ films via atomic layer deposition(ALD) technique often produces the dielectrics with a poor quality,which hinders its integration in modern semiconductor *** pretreatment approaches,such as chemical functionalization with ozone and plasma treatments,would inevitably degrade the quality of the underlying ***,we tackled this problem by utilizing an effective and convenient physical *** detail,the graphene surface was pretreated with the deposition of thermally evaporated ultrathin Al metal layer prior to the Al2O3 growth by *** the device was placed in a drying oven for 30 min to be naturally oxidized as a seed *** the assistance of an Al oxide seed layer,pinhole-free Al2O3 dielectrics growth on graphene was *** detective defects or disorders were introduced into graphene by Raman ***,our fabricated graphene topgated field effect transistor exhibited high mobility(~6200 cm2V-1s-1) and high transconductance(~117 μS).Thin dielectrics demonstrated a relative permittivity of 6.5 over a large area and a leakage current less than1.6 p A/μ*** results indicate that Al oxide functionalization is a promising pathway to achieve scaled gate dielectrics on graphene with high performance.

关键词： bonds utilizing convenient

Cloning,Characterization and Primary Function Study of a Novel Gene,Cymg1,Related to Family 2 Cystatins

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Acta Biochimica et Biophysica Sinica 2005年第1期37卷 11-18页

作者： Yang XIANG Dong-Song NIE Jian WANG Xiao-Jun TAN yun deng Shu-Wei LUO Guang-Xiu LU~* Human Reproductive and Stem Cell Engineering Institute,Central South University,Changsha 410078,China Human Reproductive and Stem Cell Engineering Institute Central South UniversityChangsha 410078 China

Cystatins are cysteine proteinase inhibitors,We found two expression sequence tags (ESTs),CA463109 and AV042522,from a mouse testis library using Digital differential display (DDD).By electricalhybridization,a novel gene,Cymgl(GenBank accession ***600990),which has a full length of 0.78 kb,and contains four exons and three introns,was cloned from a mouse testis eDNA *** gene is locatedin the 2G3 area of chromosome *** full eDNA encompasses the entire open reading frame,encoding 141amino acid *** protein has a cysteine protease inhibitor domain that is related to the family 2cystatins but lacks critical consensus sites important for cysteine protease *** characteristicsare seen in the CRES subfamily,which are related to the family 2 cystatins and are expressed specifically inthe male reproductive ***1 has a 44%(48/108)identity with mouse CRES and 30%(42/140)identity with mouse cystatin *** blot analysis showed that the Cymgl is specifically expressed inadult mouse *** location studies showed that the GFP-tagged CYMG 1 protein was localized in thecytoplasm of HeLa cells,lmmunohistochemistry revealed that the CYMG1 protein was expressed in mousetestes spermatogonium,spermatocytes,round spermatids,elongating spermatids and ***-PCRresults also showed that Cymgl was expressed in mouse testes and *** Cymgl expressionlevel varied in different developmental stages:it was low 1 week postpartum,steadily increased 2 to 5 weekspostpartum,and was highest 7 weeks *** expression level at 5 weeks postpartum was main-tained during 13 to 57 weeks *** Cymgl expression level in the testes over different develop-mental stages correlates with the mouse spermatogenesis and sexual maturation *** these indicatethat Cymgl might play an important role in mouse spermatogenesis and sexual maturation. Cystatins are cysteine proteinase inhibitors,We found two expression sequence tags(EST

关键词： cystatins gene cloning testis spermatogenesis sexual maturation

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基于高光谱分析技术的桉树叶片黄化识别

广西林业科学 2024年第1期53卷 94-99页

作者：赵隽宇石媛媛杨瑞青邓昀程小辉陈守学曹继钊唐健广西壮族自治区林业科学研究院、广西林用新型肥料研发中心广西南宁530002 广西华沃特集团股份有限公司广西南宁530025 桂林理工大学广西桂林541004

桉树黄化病是一种较特殊的生理性病害,存在一定的突发性和随机性,及时发现并补充养分可大幅减少病害带来的损失。采集桉树黄化叶片、未发病叶片和正常叶片,采用高光谱仪测定不同叶片的光谱特征,基于偏最小二乘法判别分析(Partial Least ... 详细信息

桉树黄化病是一种较特殊的生理性病害,存在一定的突发性和随机性,及时发现并补充养分可大幅减少病害带来的损失。采集桉树黄化叶片、未发病叶片和正常叶片,采用高光谱仪测定不同叶片的光谱特征,基于偏最小二乘法判别分析(Partial Least Squares Discriminant Analysis,PLS-DA)和正交偏最小二乘法判别分析(Orthogonal Partial Least Squares Dis-criminant Analysis,OPLS-DA)方法,分别建立判别分析模型,对比模型判别效果。结果表明,不同叶片光谱反射曲线呈相同趋势,反射率差异明显,差异较大的波段主要为近红外波段800~1260、1400~1720和2000~2400 nm,受病害影响叶片的原始光谱反射率明显高于正常叶片;对数变换可在一定程度上减少光谱数据冗余量,突出差异;两种线性判别分析方法均能识别潜在黄化叶片,Log-OPLS-DA的判别效果更好,模型R2为0.91,RMSE为0.203。高光谱分析技术结合OPLS-DA对桉树黄化叶片具有一定的预测和识别潜力。

关键词：高光谱黄化叶片无损检测病虫害防治桉树人工林

A case of herpesviral corneal endotheliitis presenting with corneal endothelial defect

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International Journal of Ophthalmology(English edition) 2024年第7期17卷 1375-1378页

作者： Yan-Xia Chen Han deng Xiao-yun Ke Min Fu Department of Ophthalmology Women and Children’s HospitalSchool of MedicineXiamen UniversityXiamen 361003Fujian ProvinceChina Department of Ophthamology Zhujiang HosptialSouthern Medical UniversityGuangzhou 510280Guangdong ProvinceChina

Dear Editor,Corneal endotheliitis,first reported in 1982 as an autoimmune disorder by Khodadoust and Attarzadeh[1],is now recognized to be frequently induced by viral infections,including varicella zoster virus(VZV),cytomegalovirus(CMV)and herpes simplex virus(HSV),that can cause inflammatory and immune-related injuries or direct damages to corneal endothelial cells[2-3].In cases with this type of endotheliitis of viral origin,manifestations may include conjunctival ciliary congestion or mixed congestion,corneal subepithelial bulla,localized corneal edema,keratic precipitates(KP)characteristic to the edema area,puckered posterior elastic lamina,mild anterior chamber reaction,intraocular pressure(IOP)elevation in presence of inflammation in trabecular meshwork,and endothelial decompensation[3-4].Accurate diagnosis of viral corneal endotheliitis is challenging due to its complex presentation,leading to a high misdiagnosis *** the best of our knowledge,there was no previous report of viral corneal endotheliitis presenting with corneal endothelial defects in the English literature.

关键词： corneal injuries herpes

Molecular Cloning of a Novel Mouse Testis-specific Spermatogenic Cell Apoptosis Inhibitor Gene mTSARG7 as a Candidate Oncogene

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Acta Biochimica et Biophysica Sinica 2005年第6期37卷 396-405页

作者： Xiao-Jun TAN Xiao-Wei XING Lu-yun LI Zhao-Di WU Chang-Gao ZHONG Dong-Song NIE Jun-Jiang FU Yang XIANG yun deng Guang-Xiu LU Institute of Human Reproductive and Stem cell Engineering, Central South University, Changsha 410078,China Institute of Human Reproductive and Stem cell Engineering Central South University Changsha 410078 China

A novel mouse gene,mTSARG7(GenBank accession ***489184),with a full cDNA lengthof 2279 bp and containing 12 exons and 11 introns,was cloned from a mouse expressed sequence tag(GenBank accession ***644543)that was significantly up-regulated in *** gene waslocated in mouse chromosome 8A1.3 and encoded a protein containing 403 amino acid residues that was anew member of the acyltransferase family because the sequence contained the highly conserved phosphateacyltransferase(PlsC)domain existing in all acyltransferase-like *** mTSARG7 protein and AU041707protein shared 83.9% identity in 402 amino acid *** of the mTSARG7 gene was restrictedto the mouse *** results of the in situ hybridization analysis revealed that the mTSARG7 mRNA wasexpressed in mouse spermatogonia and *** localization studies showed that the EGFP-tagged mTSARG7 protein was localized in the cytoplasm of GC-1 spg *** mTSARG7 mRNA expres-sion was initiated in the mouse testis in the second week after birth,and the expression level increasedsteadily with spermatogenesis and sexual maturation of the *** results of the heat stress experimentshowed that the mTSARG7 mRNA expression gradually decreased as the heating duration ***3.1 Hygro(-)/mTSARG7 plasmid was constructed and introduced into GC-1 spg cells by *** mTSARG7 can accelerate GC-1 spg cells,causing them to traverse the S-phase and enterthe G2-phase,compared with the control group where this did not occur as there was no transfection *** conclusion,our results suggest that this gene may play an important role in spermatogenesisand the development of cryptorchid testes,and is a testis-specific apoptosis candidate oncogene.

关键词： mTSARG7 testis spermatogenesis oncogene

Preparation of tungsten-particle-reinforced Zr-based bulk metallic glass composites by two-step spark plasma sintering:microstructure evolution,densification mechanism and mechanical properties

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Rare Metals 2024年第4期43卷 1793-1808页

作者： yun-Fei Ma Pan Gong Mao Zhang Hui-EHu Zhen Peng Xiao Xu Xin Wang Mehdi Malekan Xue-Feng Tang Lei deng Jun-Song Jin Xin-yun Wang State Key Laboratory of Materials Processing and Die and Mould Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhan430074China Research Institute of Huazhong University of Science and Technology in Shenzhen Shenzhen518057China Department of Chemistry and Materials Naval University of EngineeringWuhan430032China School of Materials Science and Engineering Jiangsu UniversityZhenjiang212013China College of Machine Shanghai Dianji UniversityShanghai201306China School of Metallurgy and Materials Engineering College of EngineeringUniversity of TehranTehran 11155-4563Iran

A new two-step spark plasma sintering(TSS)process with low-temperature pre-sintering and high-temperature final sintering has been successfully applied to prepare the tungsten-particle(Wp)-reinforced bulk metallic glass composites(Wp/BMGCs).Compared to normal spark plasma sintering(NS),the densification rate and relative density of Wp/BMGCs can be improved by selecting TSS with appropriate sintering pressure in the low temperature pre-sintering ***,the compressive strength and plastic strain of 30%Wp/BMGCs prepared by TSS are both higher than those of the samples prepared by *** TSS process can significantly enhance the compressive strength of 30%Wp/BMGCs by 12%and remarkably increase the plastic strain by 50%,while the trend is completely opposite for 50%Wp/***-in situ experiments and finite element simulations reveal that uneven temperature distribution among particles during low-temperature pre-sintering causes local overheating at contact points between particles,accelerating formation of sintering neck between particles and plastic deformation of *** the volume fraction of Wp is low,TSS can improve the interface bonding between particles by increasing the number of sintering *** makes the fracture mode of Wp/BMGCs being predominantly transgranular ***,as the volume fraction of Wp increases,the adverse effects of Wp plastic deformation are becoming more and more *** aggregated Wp tends to form a solid"cage structure"that hinders the bonding between particles at the interface;correspondingly,the fracture behavior of Wp/BMGCs is mainly dominated by intergranular ***,reducing the sintering pressure during the low-temperature pre-sintering stage of TSS has been shown to effectively decrease plastic deformation in Wp,resulting in a higher degree of densification and better mechanical properties.

关键词： Bulk metallic glass composites Tungsten particle Two-step spark plasma sintering Densification mechanism Mechanical properties

改性类黑精-刺梨渣提取物-淀粉-明胶复合保鲜膜的制备及其对核桃贮藏品质的影响

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塑料包装 2024年第3期34卷 29-35,20页

作者：尹浩张若妍张一郭灵茜钟宇王丹凤章敏燕赵芳邓云上海交通大学农业与生物学院食品科学与工程系上海200240 云南省洱源县检验检测院云南洱源671200

为探究改性类黑精和刺梨渣提取物添加量对淀粉-明胶复合保鲜膜性能的影响及其对核桃的保鲜效果,以淀粉-明胶为基质,添加类黑精和刺梨渣提取物,筛选最佳成膜配方,并应用于核桃保鲜。结果表明当类黑精和刺梨渣提取物添加量为0.75%和0.4%时... 详细信息

为探究改性类黑精和刺梨渣提取物添加量对淀粉-明胶复合保鲜膜性能的影响及其对核桃的保鲜效果,以淀粉-明胶为基质,添加类黑精和刺梨渣提取物,筛选最佳成膜配方,并应用于核桃保鲜。结果表明当类黑精和刺梨渣提取物添加量为0.75%和0.4%时,成膜性能最佳;复合膜抗氧化能力与类黑精和提取物添加量成正比;复合膜有效保鲜核桃。因此,添加改性类黑精和刺梨渣提取物的复合保鲜膜成膜性良好,有利于延长坚果类食品货架期。

关键词：副产物利用可食性复合膜抗氧化活性包装

Microstructure and mechanical properties of Co-28Cr-6Mo-0.22C investment castings by current solution treatment

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China Foundry 2024年第4期21卷 369-378页

作者： Ze-yu Dan Jun Liu Jian-lei Zhang Yan-hua Li Yuan-xin deng yun-hu Zhang Chang-jiang Song Center for Advanced Solidification Technology School of Materials Science and EngineeringShanghai UniversityShanghai 200444China Wuxi CastPro Precision Co. Ltd.Wuxi 214105JiangsuChina

This study examined the impact of current solution treatment on the microstructure and mechanical properties of the Co-28Cr-6Mo-0.22C alloy investment *** findings reveal that the current solution treatment significantly promotes the dissolution of carbides at a lower *** optimal conditions for solution treatment are determined as a solution temperature of 1,125°C and a holding time of 5.0 *** these parameters,the size and volume fraction of precipitated phases in the investment castings are measured as6.2μm and 1.1vol.%.The yield strength,ultimate tensile strength,and total elongation of the Co-28Cr-6Mo-0.22C investment castings are 535 MPa,760 MPa,and 12.6%,*** values exceed those obtained with the conventional solution treatment at 1,200°C for 4.0 *** findings suggest a phase transformation of M_(23)C_(6)→σ+C following the current solution treatment at 1,125°C for 5.0 *** comparison,the traditional solution treatment at 1,200°C for 4.0 h leads to the formation of M_(23)C_(6)and M_(6)C *** is noteworthy that the non-thermal effect of the current during the solution treatment modifies the free energy of both the matrix and precipitation *** modification lowers the phase transition temperature of the M_(23)C_(6)→σ+C reaction,thereby facilitating the dissolution of *** a result,the current solution treatment approach achieves carbide dissolution at a lower temperature and within a significantly shorter time when compared to the traditional solution treatment methods.

关键词： CoCrMo alloy investment castings current solution treatment microstructure mechanical property carbide