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Anti-biofilm and remineralization effects of chitosan hydrogel containing amelogeninderived peptide on initial caries lesions

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Regenerative Biomaterials 2018年第2期5卷 69-76页

作者： Qian Ren Zhongcheng Li Longjiang Ding Xiuqing Wang yumei niu Xi Qin Xuedong Zhou Linglin Zhang State Key Laboratory of Oral Diseases National Clinical Research Center for Oral Diseasesand Department of Cariology and EndodonicsWest China Hospital of StomatologySichuan UniversityNo.143rd SectionRenmin Nan LuChengduPeople’s Republic of China

In this study,we have designed a more clinically powerful anti-caries treatment by applying the amelogenin-derived peptide QP5 to the antibacterial carrier material chitosan in a hydrogel(CS-QP5 hydrogel),and characterized its effects on the inhibition of a cariogenic biofilm and the promotion of the remineralization of the initial caries *** results indicated that the CS-QP5 hydrogel sustainably inhibited the growth of the Streptococcus mutans biofilm,lactic acid production and the metabolic activity over a prolonged period of ***,the CS-QP5 hydrogel promoted the remineralization of early enamel lesions,which were indicated by surface microhardness(,polarized light microscopy and transverse *** conclusion,the CS-QP5 hydrogel shows good potential for caries control in the clinic because of its antibacterial effects as well as the remineralization of initial enamel carious lesions even in a biofilm model over a prolonged period of time.

关键词： caries amelogenin-derived peptide chitosan remineralization

Remineralization of enamel caries by an amelogenin-derived peptide and fluoride in vitro

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Regenerative Biomaterials 2020年第3期7卷 283-292页

作者： Longjiang Ding Sili Han Kun Wang Sainan Zheng Wenyue Zheng Xiu Peng yumei niu Wei Li Linglin Zhang State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesDepartment of Cariology and EndodonticsWest China Hospital of StomatologySichuan UniversityChengduChina

Dental caries is one of the most common oral diseases in the *** study was tantamount to investigate the combinatory effects of an amelogenin-derived peptide(called QP5)and fluoride on the remineralization of artificial enamel *** peptide QP5 was synthesized and characterized,and the binding capability of the peptide on hydroxyapatite(HA)and demineralized tooth enamel surface was ***,the mineralization function of the peptide and fluoride was studied through the spontaneous mineralization testing and remineralization on enamel caries in ***,the novel peptide QP5 could bind on the hydroxyapatite and demineralized tooth enamel ***,QP5 can transitorily stabilize the formation of amorphous calcium phosphate and direct the transformation into hydroxyapatite crystals alone and in combination with *** addition,compared to blocks treated by peptide QP5 alone or fluoride,the sample blocks showed significantly higher surface microhardness,lower mineral loss and shallower lesion depth after treatment with a combination of QP5 and fluoride at high or low *** peptide QP5 could control the crystallization of hydroxyapatite,and combinatory application of peptide QP5 and fluoride had a potential synergistic effect on the remineralization of enamel caries.

关键词： enamel caries remineralization amelogenin peptide fluoride

PEP06 polypeptide 30 is a novel clusterdissociating agent inhibiting αv integrin/FAK/Src signaling in oral squamous cell carcinoma cells

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Acta Pharmaceutica Sinica B 2019年第6期9卷 1163-1173页

作者： Gulnara Tuguzbaeva Er Yue Xi Chen Lina He Xinlei Li Jiaming Ju Ying Qin Valentin Pavlov Yanjie Lu Wenting Jia Yunlong Bai yumei niu Baofeng Yang Central Laboratory of Scientific Research Bashkir State Medical UniversityUfa 450008Russian Federation Department of Pharmacology(State-Province Key Laboratories of BiomedicineePharmaceutics of China Key Laboratory of Cardiovascular ResearchMinistry of Education)College of PharmacyHarbin Medical UniversityHarbin 150081China Translational Medicine Research and Cooperation Center of Northern China Heilongjiang Academy of Medical SciencesHarbin 150081China Department of Endodontics the First Affiliated Hospital of Harbin Medical UniversityHarbin 150081China Department of Orthopedic Dentistry and Maxillofacial Surgery Bashkir State Medical UniversityUfa 450008Russian Federation

Collectively migrating tumor cells have been recently implicated in enhanced metastasis of epithelial *** oral squamous cell carcinoma(OSCC),av integrin is a crucial mediator of multicellular clustering and collective movement in vitro;however,its contribution to metastatic spread remains to be *** to the emerging therapeutic concept,dissociation of tumor clusters into single cells could significantly suppress metastasis-seeding ability of *** study aimed to investigate the anti-OSCC potential of novel endostatin-derived polypeptide PEP06 as a clusterdissociating therapeutic agent in ***,we found marked enrichment ofαv integrin in collectivelyinvading multicellular clusters in human *** study revealed that metastatic progression of OSCC was associated with augmented immunostaining of av integrin in cancerous *** PEP06treatment,cell clustering on fibronectin,migration,multicellular aggregation,anchorage-independent survival and colony formation of OSCC were significantly ***,PEP06 suppressed av integrin/FAK/Sre signaling in OSCC ***06-induced loss of active Src and E-cadherin from cell-cell contacts contributed to diminished collective migration of OSCC in ***,these results suggest that PEP06 polypeptide 30 inhibiting av integrin/FAK/Src signaling and disrupting E-cadherin-based intercellular junctions possesses anti-metastatic potential in OSCC by acting as a cluster-dissociating therapeutic agent.

关键词： Oral squamous cell carcinoma Tumor cell clusters Collective migration Metastasis αv integrin/FAK/RC signaling RGD

Expert consensus on dental caries management

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International Journal of Oral Science 2022年第2期14卷 132-139页

作者： Lei Cheng Lu Zhang Lin Yue Junqi Ling Mingwen Fan Deqin Yang Zhengwei Huang yumei niu Jianguo Liu Jin Zhao Yanhong Li Bin Guo Zhi Chen Xuedong Zhou State Key Laboratory of Oral Diseases&National Clinical Research Centre for Oral Diseases&Department of Cariology and Endodontics West China Hospital of StomatologySichuan UniversityChengduChina The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)&Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of StomatologyWuhan UniversityWuhanChina Department of Cariology and Endodontology Peking University School and Hospital of Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Research of Oral Biomaterials and Digital Medical Devices&Beijing Key Laboratory of Digital StomatologyPeking UniversityBeijingChina Department of Operative Dentistry and Endodontics Hospital of StomatologyGuanghua School of StomatologySun Yatsen University and Guangdong Provincial Key Laboratory of StomatologySun Yat‑Sen UniversityGuangzhouGuangdongChina School of Medicine Jianghan UniversityWuhanChina College of Stomathology Chongqing Medical UniversityChongqingChina Department of Endodontics Shanghai Ninth People’s HospitalCollege of StomatologyShanghai JiaoTong University School of MedicineNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of Stomatology&Shanghai Research Institute of StomatologyShanghai Jiao Tong UniversityShanghaiChina Department of Endodontics The First Affiliated Hospital of Harbin Medical University&Department of EndodonticsSchool of StomatologyHarbin Medical UniversityHarbinChina Key Laboratory of Oral Disease Research School of StomatologyZunyi Medical UniversityZunyiChina Department of Endodontics First Affiliated Hospital of Xinjiang Medical Universityand College of Stomatology of Xinjiang Medical UniversityUrumqiChina Affiliated Stomatological Hospital of Kunming Medical University KunmingYunnanChina Department of Stomatology the First Medical CentreChinese PLA General HospitalBeijingChina

Dental Caries is a kind of chronic oral disease that greatly threaten human being’s *** dentists and researchers struggled for decades to combat this oral disease,the incidence and prevalence of dental caries remain quite ***,improving the disease management is a key issue for the whole population and life cycle management of dental *** clinical difficulty assessment system of caries prevention and management is established based on dental caries diagnosis and *** should perform oral examination and establish dental records at each *** treatment plan is made on the base of caries risk assessment and carious lesion activity,we need to work out patient-centered and personalized treatment planning to regain oral microecological balance,to control caries progression and to restore the structure and function of the carious *** the follow-up visits are made based on personalized caries *** expert consensus mainly discusses caries risk assessment,caries treatment difficulty assessment and dental caries treatment plan,which are the most important parts of caries management in the whole life cycle.

关键词： prevention difficulty caries

从标记过度与标记缺失看情状体对习得一般过去时标记的影响

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外语教学 2007年第1期28卷 65-68页

作者：牛毓梅山东大学外国语学院山东济南250100

本研究从一般过去时标记在使用中存在的问题(标记过度和标记缺失现象)出发对情状体假设作了进一步的验证。本文描述并分析了41篇英语叙述体作文,结果显示,标记过度和标记缺失现象最多出现于状态动词,其次是活动动词,然后是完结动词和达... 详细信息

本研究从一般过去时标记在使用中存在的问题(标记过度和标记缺失现象)出发对情状体假设作了进一步的验证。本文描述并分析了41篇英语叙述体作文,结果显示,标记过度和标记缺失现象最多出现于状态动词,其次是活动动词,然后是完结动词和达成动词,所反映的习得顺序基本支持情状体假设的第一个习得过程。与情状体假设的不符之处是,本研究中一般过去时标记最先标记终结性动词而非仅仅是瞬时性动词。另外,本文还验证了系动词be作为时态承载动词在习得过程中的特殊性。

关键词：情状体情状体假设一般过去时标记标记过度标记缺失

Experimental research for specific down-regulated expression of p53 gene by individual antisense RNA in vitro

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The Chinese-German Journal of Clinical Oncology 2007年第1期6卷 62-67页

作者： YahongWang Shaofeng Xu Yuanyuan Zhang Bin Zhang yumei Feng Ruifang niu Li Fu Department of Pathology and Research Laboratory of Breast Cancer Key Laboratory of Breast Cancer Prevention and Therapy of the Ministry of Education Tianjin Medical University Tianjin 300060 China

Objective： To investigate the specific blockage effect of individual antisense RNA on mutant p53 gene in vitro. Methods： The single strand antisense transcription system containing mt-p53 exon 8 sequence （pGEM3zf（＋/-）p53exon8） was constructed. The ligation of antisense RNAwith mt-p53 gene was confirmed by in situ hybridization; MDA-MB-231 human breast cancer cells were transfected with ASp53exon8＇RNA cotionic liposome-mediated. Expression of mt-p53 protein was examined by immunocytochemical staining and Western blot. Cell proliferation was evaluated by MTT assay; Cell cycle distribution was determined by flow cytometry （FCM）; Apoptosis was observed by TUNEL. Results： In transfected MDA-MB-231 cells, hybridization signals were observed in cytoplasm. ASp53exon8＇RNA transfection induced inhibition of cell proliferation, G2/M phase arrest and increasing apoptotic rates. In addition, expression of p53 protein was down-regulated. Conclusion： pGEM3zf（＋/-）p53exon8 was well constructed and ASp53exon8＇RNA can block mt-p53 gene expression specifically and then inhibit MDA-MB-231 cell proliferation in vitro, which may serve as therapeutic means for human malignancy.

关键词： individual antisense RNA mutant p53 gene specific blockage mutant protein expression

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碳基材料在周围神经损伤修复中的应用

口腔医学研究 2023年第3期39卷 187-192页

作者：牛玉梅孙靖宣哈尔滨医科大学附属第一医院、哈尔滨医科大学口腔医学院黑龙江哈尔滨150001

周围神经损伤严重影响患者身心健康,尤其是离断性周围神经损伤。自体神经移植作为目前临床治疗常用方法,存在着供体来源有限、供体区失神经障碍等局限性。人工移植材料的开发是解决上述问题的关键,近年来碳基材料在周围神经损伤修复治... 详细信息

周围神经损伤严重影响患者身心健康,尤其是离断性周围神经损伤。自体神经移植作为目前临床治疗常用方法,存在着供体来源有限、供体区失神经障碍等局限性。人工移植材料的开发是解决上述问题的关键,近年来碳基材料在周围神经损伤修复治疗中展现出显著优势。本综述从该角度出发,对碳基材料在周围神经损伤中的研究进展加以总结并对其未来在周围神经损伤尤其是面神经损伤领域的应用加以展望,为临床治疗离断性面神经损伤提供新思路。

关键词：周围神经周围神经损伤碳基材料组织工程面神经损伤修复

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牙髓干细胞对面神经损伤修复研究进展

口腔医学研究 2021年第3期37卷 185-190页

作者：牛玉梅孙翔宇哈尔滨医科大学附属第一医院哈尔滨医科大学口腔医学院黑龙江哈尔滨150001

面神经损伤严重影响患者的身心健康,近些年,利用干细胞治疗神经损伤受到广泛关注。牙髓干细胞因其具有多种优势,在神经损伤的修复治疗中显示出巨大的优势,为面神经损伤的治疗提供了新的思路。本文将概述牙髓干细胞对面神经损伤修复的研... 详细信息

面神经损伤严重影响患者的身心健康,近些年,利用干细胞治疗神经损伤受到广泛关注。牙髓干细胞因其具有多种优势,在神经损伤的修复治疗中显示出巨大的优势,为面神经损伤的治疗提供了新的思路。本文将概述牙髓干细胞对面神经损伤修复的研究进展和应用前景。

关键词：牙髓干细胞面神经损伤损伤修复

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氢氧化钙表面粘接处理对其压缩强度的影响

实用口腔医学杂志 2017年第6期33卷 754-757页

作者：徐莹郭剑苓牛玉梅张琳潘爽哈尔滨医科大学附属第一医院口腔牙体牙髓科 150001

目的:比较化学固化氢氧化钙(Dycal)和光固化氢氧化钙(Calcimol LC)表面粘接处理后对其压缩强度的影响。方法:制备实验试件32个,分4组(n=8):A组为Dycal组;B组为Calcimol LC组;C组为粘接处理的Dycal组;D组为粘接处理的Calcimol LC组。采... 详细信息

目的:比较化学固化氢氧化钙(Dycal)和光固化氢氧化钙(Calcimol LC)表面粘接处理后对其压缩强度的影响。方法:制备实验试件32个,分4组(n=8):A组为Dycal组;B组为Calcimol LC组;C组为粘接处理的Dycal组;D组为粘接处理的Calcimol LC组。采用德国Zwick电子万能试验机对各个试件进行压缩强度检测。结果:A、B、C、D组的平均压缩强度值分别为(110.48±19.9)、(162.6±7.8)、(136.8±17.2)和(223±31.1)MPa。D组压缩强度值高于其他3组(P<0.05),B组的压缩强度值高于A组(P<0.05),其他组间无明显统计学差异。结论:光固化氢氧化钙的压缩强度高于化学固化氢氧化钙,光固化氢氧化钙表面粘接处理后,提高了充填体的压缩强度,而化学固化氢氧化钙表面粘接处理对其压缩强度没有影响。

关键词：氢氧化钙光固化化学固化压缩强度