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Surface modification of titanium substrate via combining photothermal therapy and quorum-sensing-inhibition strategy for improving osseointegration and treating biofilm-associated bacterial infection

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Bioactive Materials 2022年第12期7卷 228-241页

作者： Jingwei hu Yao Ding Bailong Tao Zhang Yuan yulu Yang Kun Xu Xuan Li Peng liu Kaiyong Cai Key Laboratory of Biorheological Science and Technology Ministry of EducationCollege of BioengineeringChongqing UniversityChongqing400044China Laboratory Research Center The First Affiliated Hospital of Chongqing Medical UniversityChongqing400016China Institute of Medical Research Northwestern Polytechnical UniversityXi’an710072China

Insufficient osseointegration and biofilm-associated bacterial infection are important challenges for clinical application of titanium(Ti)-based ***,we constructed mesoporous polydopamine(MPDA)nanoparticles(NPs)loaded with luteolin(LUT,a quorum sensing inhibitor),which were further coated with the shell of calcium phosphate(CaP)to construct MPDA-LUT@CaP ***,MPDA-LUT@CaP NPs were immobilized on the surface of Ti *** acidic environment of bacterial biofilm-infection,the CaP shell of MPDA-LUT@CaP NPs was rapidly degraded and released LUT,Ca^(2+)and PO_(4)^(3-)􀀀from the surface of Ti *** could effectively inhibit and disperse ***,under near-infrared irradiation(NIR),the thermotherapy induced by the photothermal conversion effect of MPDA destroyed the integrity of the bacterial membrane,and synergistically led to protein leakage and a decrease in ATP *** with photothermal therapy(PTT)and quorum-sensing-inhibition strategy,the surface-functionalized Ti substrate had an antibacterial rate of over 95.59%against Staphylococcus aureus and the elimination rate of the formed biofilm was as high as 90.3%,so as to achieve low temperature and efficient treatment of bacterial biofilm *** importantly,the modified Ti implant accelerated the growth of cell and the healing process of bone tissue due to the released Ca^(2+)and PO_(4)^(3-)􀀀.In summary,this work combined PTT with quorum-sensing-inhibition strategy provides a new idea for surface functionalization of implant for achieving effective antibacterial and osseointegration capabilities.

关键词： Titanium implant Biofilm infection Osseointegration Photothermal therapy Quorum sensing

Percutaneous absorption and brain distribution facilitation of borneol on tetramethylpyrazine in a microemulsion-based transdermal therapeutic system

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Asian Journal of Pharmaceutical Sciences 2019年第3期14卷 305-312页

作者： Xiaoge hu Ning Cheng Jihui Zhao Xianghua Piao yulu Yan Qibo Zhang Kuan Zhou Yongtai Zhang Nianping Feng School of Pharmacy Shanghai University of Traditional Chinese Medicine

In this study, we show that the percutaneous absorption and brain distribution of tetramethylpyrazine(TMP) is enhanced when combined with borneol(BN) in a microemulsionbased transdermal therapeutic system(ME-TTS). The formulation of the TMP and BN microemulsion(TEM-BN-ME) was optimized in skin permeation studies in vitro following a uniform experimental design. Male Sprague-Dawley rats were used for the in vivo pharmacokinetic and tissue distribution studies of TMP-BN-ME-TTS. In the pharmacokinetic study, the TMP-BN-ME-TTS treated rats had significantly higher( P < 0.05) C max and AUC of TMP than the TMP-ME-TTS treated rats, indicating that BN improves the rate and extent of TMP percutaneous absorption. In the tissue distribution study, the AUC of TMP in brain was significantly higher in the TMP-BN-ME-TTS group( P < 0.05), indicating that BN facilitates the distribution of TMP in brain. In summary, BN enhanced the percutaneous absorption and brain distribution of TMP in a microemulsion-based transdermal therapeutic system.

关键词： Tetramethylpyrazine Borneol Microemulsion Percutaneous absorption Brain distribution

棉酚对雄性肉仔鸡生长性能、血清抗氧化能力及肾脏病理组织形态的影响

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畜牧与饲料科学 2023年第6期44卷 22-30页

作者：李啊诗刘盼张新楠胡雨露李玉杰赵扬吴尚泽贺建忠张梦迪塔里木大学动物科学与技术学院新疆阿拉尔843300

[目的]研究棉酚对不同生长阶段雄性肉仔鸡生长性能、血清抗氧化能力及肾脏病理组织形态的影响,为肉鸡养殖中棉籽粕的合理应用积累基础试验数据。[方法]选取48只同批次1日龄健康雄性良凤花肉仔鸡,根据体重相近原则分为4组:对照组(C)、低... 详细信息

[目的]研究棉酚对不同生长阶段雄性肉仔鸡生长性能、血清抗氧化能力及肾脏病理组织形态的影响,为肉鸡养殖中棉籽粕的合理应用积累基础试验数据。[方法]选取48只同批次1日龄健康雄性良凤花肉仔鸡,根据体重相近原则分为4组:对照组(C)、低剂量组(L)、中剂量组(M)和高剂量组(H),每组4个重复,每个重复3只。在饲喂基础日粮基础上,C组以及L组、M组、H组每天分别灌胃蒸馏水以及100、150、200 mg/(kg·BW)棉酚1次,直至20日龄,共灌胃20 d;试验期间,记录各组肉仔鸡的采食及增重情况,计算不同生长阶段的料重比;分别于6、11、16和21日龄采集肉仔鸡血液样本,测定血清过氧化氢酶(catalase,CAT)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力;于21日龄采集肾脏组织样本,制备HE染色切片,观察肾脏病理组织学变化。[结果]1~15日龄阶段,L组、M组和H组的料重比极显著(PH组>L组。[结论]从1日龄开始给雄性肉仔鸡连续灌喂20 d剂量为100、150和200 mg/(kg·BW)的棉酚,可降低其生长性能及血清抗氧化能力,并对肾脏造成损伤;随着灌胃时间的延长,以150 mg/(kg·BW)的剂量影响最为明显。

关键词：棉酚雄性肉仔鸡生长性能血清抗氧化酶肾脏病变

A HAase/NIR responsive surface on titanium implants for treating bacterial infection and improving osseointegration

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Journal of Materials Science & Technology 2023年第12期143卷 93-106页

作者： Dan Li Danyang Wang Ye He Bailong Tao Xiaoxia Liu yulu Yang Lu Tan Yuchen Zhang Jingwei hu Weihu Yang Yu Tang Kaiyong Cai Key Laboratory of Biorheological Science and Technology Ministry of EducationCollege of BioengineeringChongqing UniversityChongqing 400044PR.China Laboratory Research Center The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR.China School of Life Science Chongqing UniversityChongqing 400044PR.China Department of Orthopedics The Second Affiliated Xinqiao Hospital of Army Medical UniversityChongqing 400030PR.China

Bacterial infection and insufficient osseointegration are critical factors affecting the long-term success of titanium-based ***,the direct application of antibiotic on Ti implants easily leads to poor cytocompatibility,as well as the production of drug-resistant ***,in this work,we designed a prospective antibacterial strategy by combining photothermal and ciprofloxacin(CIP).The synergistic effect of photothermal and antibiotic may provide an effective bacteriostatic efficacy without sacrificing osteogenesis at a mild condition of moderate temperature and less ***,CIP was loaded into mesoporous polydopamine(MPDA)nanoparticles(MPDA@CIP),which were anchored on the surface of titanium and finally covered with sodium hyaluronate-catechol(HAc)*** hydrophilic HAc layer could inhibit the early adhesion of bacteria,and some bacteria could secrete bacterial hyaluronidase to accelerate the degradation of *** enabled smart enzyme-triggered release of antimicrobials at the site of infection on-demand and avoided unwanted side effects on normal *** addition,NIR light irradiation had a positive influence on both CIP release and MPDA nanoparticle’s photothermal ***,before anchoring MPDA@CIP,by the construction of hydroxyapatite microstructure on Ti sur-face with micro-arc oxidation and alkali heat treatment,the ability of bone formation of Ti could be promoted *** in vitro as well as in vivo assays demonstrated that functional Ti has an excellent antibacterial effect and osteogenic ability.

关键词： Titanium implant Antibiosis Hyaluronidase response Mesoporous polydopamine nanoparticles

HIV-1感染小鼠动物模型建立及体内整合前病毒定量分析

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生物工程学报 2024年第7期40卷 2195-2210页

作者：高阳刘嘉睿王长俊马妍周颖董廷王涛孙语璐顾潮江武汉科技大学生命科学与健康学院湖北武汉430065

近年来,多种新型抗人类免疫缺陷病毒(human immunodeficiency virus,HIV)疗法如基因治疗、广谱中和抗体以及衍生的嵌合抗原受体T细胞(chimeric antigen receptor modified T cell,CAR-T)疗法均迫切需要理想的动物模型及用于精准定量病... 详细信息

近年来,多种新型抗人类免疫缺陷病毒(human immunodeficiency virus,HIV)疗法如基因治疗、广谱中和抗体以及衍生的嵌合抗原受体T细胞(chimeric antigen receptor modified T cell,CAR-T)疗法均迫切需要理想的动物模型及用于精准定量病毒基因组的方法。本研究通过双标HIV假病毒构建及感染获得HIV-?ENV-Jurkat-EGFP-m Cherry稳转细胞,并以该细胞基因组作为整合前病毒的标准品,建立巢式荧光定量PCR (nested quantitative polymerase chain reaction,nested-q PCR)定量HIV整合前病毒。通过尾静脉注射健康人外周血单个核细胞(peripheral blood mononuclear cell,PBMC)到NOD/Prkdcscid/IL2rgnull (NPG)小鼠,通过监测小鼠外周血中h CD45+、h CD3+、h CD4+和h CD8+人源化细胞的比例来判断人源化小鼠模型构建是否成功。腹腔注射HIV NL4-3-Nano Luc病毒,随后通过小动物活体成像及分子病毒学评价HIV体内复制,结果表明,尾静脉注射正常人PBMC细胞至小鼠内3-5周后,实验组小鼠体内均检测到人源免疫细胞的浸润,建模5周后的人源化小鼠外周血中h CD45大于25%,即人源化小鼠模型构建成功。感染27 d后小动物活体成像能检测到病毒相关萤光素酶蛋白表达,分子病毒学结果表明在脾脏中病毒总DNA、RNA和整合前病毒DNA分别达到了18 000 copies/10^(6) cells、15 000 copies/μg RNA、15 000 copies/10^(6) cell。本研究证明了通过尾静脉注射正常人PBMC,可成功建立hu PBMC-NPG/严重联合免疫缺陷(severe combined immunodeficiency,SCID)人源化小鼠模型,并且HIV病毒可成功感染该模型。本研究建立了有效测定HIV整合前病毒DNA的方法,为艾滋病体内复制水平及病毒库大小和对多种新型抗HIV疗法的治疗效果的评价奠定了基础。

关键词：人源化小鼠人类免疫缺陷病毒(HIV) NPG免疫缺陷小鼠外周血单个核细胞(PBMC)

桑黄酮G通过调控PI3K/AKT/mTOR通路抑制胃癌细胞的生长、迁移和侵袭

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南方医科大学学报 2024年第8期44卷 1476-1484页

作者：耿志军杨晶晶牛民主刘馨悦施金冉刘亦珂姚新宇张雨路张小凤胡建国蚌埠医科大学第一附属医院1中心实验室安徽蚌埠233004 蚌埠医科大学第一附属医院胃肠外科安徽蚌埠233004 蚌埠医科大学检验医学院免疫学教研室安徽蚌埠233000 炎症相关性疾病基础与转化研究安徽省重点实验室安徽蚌埠233004 蚌埠医科大学第一附属医院检验科安徽蚌埠233004

目的探讨桑黄酮G(KG)对胃癌细胞的增殖、凋亡、迁移和侵袭的作用和分子机制。方法通过CCK-8实验、细胞克隆形成实验、裸鼠背部成瘤和Ki-67免疫组织化学染色评估不同浓度KG对胃癌细胞增殖与生长的影响;应用Annexin V-FITC/PI细胞凋亡检... 详细信息

目的探讨桑黄酮G(KG)对胃癌细胞的增殖、凋亡、迁移和侵袭的作用和分子机制。方法通过CCK-8实验、细胞克隆形成实验、裸鼠背部成瘤和Ki-67免疫组织化学染色评估不同浓度KG对胃癌细胞增殖与生长的影响;应用Annexin V-FITC/PI细胞凋亡检测试剂盒、Western blotting分析凋亡相关蛋白表达和Tunel染色评估KG对胃癌细胞凋亡的影响;使用Transwell迁移和侵袭实验与Western blotting分析基质金属蛋白酶表达检测KG对胃癌细胞迁移和侵袭的作用;利用免疫印迹技术和rescue实验验证PI3K/AKT/mTOR通路在KG调控胃癌细胞的增殖、迁移和侵袭的作用。结果KG以浓度依赖性抑制胃癌细胞的增殖和减少细胞形成克隆数(P<0.05);KG上调凋亡细胞比例,促进cleaved caspase-3、Bcl2-Bax表达并下调Bcl2水平(P<0.05);KG干扰胃癌细胞的迁移和侵袭,并且抑制基质金属蛋白酶2(MMP2)和基质金属蛋白酶9(MMP9)的表达(P<0.05)。机制验证显示,KG抑制PI3K/AKT/mTOR通路的激活,且PI3K/AKT/mTOR通路的激活剂IGF-1逆转了KG对胃癌细胞增殖、迁移和侵袭的影响(P<0.05)。结论KG至少部分是通过抑制PI3K/AKT/mTOR通路的激活影响胃癌细胞的增殖、凋亡、迁移和侵袭。

关键词：胃癌桑黄酮G 恶性行为 PI3K/AKT/mTOR通路

Identification of the candidate gene controlling tiller angle in common wheat through genome-wide association study and linkage analysis

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The Crop Journal 2023年第3期11卷 870-877页

作者： Lei Zhao Canguan Wang Tongzhu Wang Jinyuan Liu Qi Qiao yulu Yang Pengyu hu Leilei Zhang Simin Zhao Daiying Chen Yan Ren Ning Zhang Zhongdong Dong Feng Chen National Key Laboratory of Wheat and Maize Crop Science/CIMMYT-China Wheat and Maize Joint Research Center/Agronomy College Henan Agricultural UniversityZhengzhou 450046HenanChina

Wheat tiller angle(TA)is an important agronomic trait that contributes to grain production by affecting plant *** also plays a crucial role in high-yield wheat *** association panel and a recombinant inbred line(RIL)population were used to map quantitative trait loci(QTL)for *** showed that 470 significant SNPs with 10.4%–28.8%phenotypic variance explained(PVE)were detected in four replicates by a genome-wide association study(GWAS).Haplotype analysis showed that the TA_Hap_4B1 locus on chromosome 4B was a major QTL to regulate wheat *** QTL were totally detected by linkage mapping with the RIL population,and ***-4B.1 identified in six environments with the PVE of 7.88%–18.82%was a major and stable QTL.A combined analysis demonstrated that both TA_Hap_4B1 and ***-4B.1 were co-located on the same ***,***-4B.1 was confirmed by bulked segregant RNA-Seq(BSR-Seq)*** analysis showed that ***-4B.1was also closely related to yield ***,Traes CS4B02G049700 was considered as a candidate gene through analysis of gene sequence and *** study can be potentially used in cloning key genes modulating wheat tillering and provides valuable genetic resources for improvement of wheat plant architecture.

关键词： Common wheat Tiller angle GWAS Linkage mapping Plant architecture

Probing dark matter particles from evaporating primordial black holes via electron scattering in the CDEX-10 experiment

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Science China(Physics,Mechanics & Astronomy) 2024年第10期67卷 83-91页

作者： Zhenhua Zhang Litao Yang Qian Yue Kejun Kang Yuanjing Li Haipeng An Greeshma C. Jianping Chang Yunhua Chen Jianping Cheng Wenhan Dai Zhi Deng Changhao Fang Xinping Geng hui Gong Qiuju Guo Tao Guo Xuyuan Guo Li He Shengming He Jinwei hu Hanxiong huang Tuchen huang Lin Jiang S.Karmakar Haubin Li Hanyu Li Jianmin Li Jin Li Qianyun Li Renmingjie Li Xueqian Li Yulan Li Yifan Liang Bin Liao FongKay Lin ShinTed Lin Jiaxuan Liu Shukui Liu Yandong Liu Yu Liu Yuanyuan Liu Hao Ma Yucai Mao Qiyuan Nie Jinhua Ning hui Pan Ningchun Qi Jie Ren Xichao Ruan Monoj Kumar Singh Tianxi Sun Changjian Tang Yang Tian Guangfu Wang Junzheng Wang Li Wang Qing Wang Yufeng Wang Yunxiang Wang Henry Tsz-King Wong Shiyong Wu Yucheng Wu Haoyang Xing Rui Xu Yin Xu Tao Xue yulu Yan Nan Yi Chunxu Yu Haijun Yu Jianfeng Yue Ming Zeng Zhi Zeng Bingtao Zhang Fengshou Zhang Lei Zhang Zhenyu Zhang Jizhong Zhao Kangkang Zhao Minggang Zhao Jifang Zhou Zuying Zhou Jingjun Zhu CDEX Collaboration Key Laboratory of Particle and Radiation Imaging(Ministry of Education)and Department of Engineering Physics Tsinghua UniversityBeijing 100084China Department of Physics Tsinghua UniversityBeijing 100084China Institute of Physics Academia SinicaTaipei 11529China NUCTECH Company Beijing 100084China YaLong River Hydropower Development Company Chengdu 610051China College of Nuclear Science and Technology Beijing Normal UniversityBeijing 100875China College of Physics Sichuan UniversityChengdu 610065China School of Physics Peking UniversityBeijing 100871China Department of Nuclear Physics China Institute of Atomic EnergyBeijing 102413China Sino-French Institute of Nuclear and Technology Sun Yet-sen UniversityZhuhai 519082China School of Physics Nankai UniversityTianjin 300071China Department of Physics Banaras Hindu UniversityVaranasi 221005India Department of Physics Beijing Normal UniversityBeijing 100875China 不详

Dark matter(DM)is a major constituent of the ***,no definite evidence of DM particles(denoted as“χ”)has been found in DM direct detection(DD)experiments to *** is a novel concept of detectingχfrom evaporating primordial black holes(PBHs).We search forχemitted from PBHs by investigating their interaction with target *** examined PBH masses range from 1×10^(15)to 7×10^(16)g under the current limits of PBH abundance *** 205.4 kg·day data obtained from the CDEX-10 experiment conducted in the China Jinping Underground Laboratory,we exclude theχ-electron(χ-e)elastic-scattering cross sectionσ_(χe)~5×10^(-29)cm^(2)forχwith a mass■keV from our *** the higher radiation background but lower energy threshold(160 eV),CDEX-10 fills a part of the gap in the previous ***(m_(χ),σ_(χe))can be determined in the future,DD experiments are expected to impose strong constraints on fPBHfor large MPBHs.

关键词： CDEX primordial black hole light dark matter particle dark matter-electron scattering high purity germanium detector

Species and Control Techniques of Main Diseases and Insect Pests of Camellia oleifera in Anhui Province

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Plant Diseases and Pests 2023年第3期14卷 1-4,13页

作者： Juanjuan hu Hao SONG Zhihua CAO yulu CHEN Jie WANG Wangsheng Zhu Dewu YAO Bangxing HAN College of Environment and Tourism West Anhui UniversityLu an 237012China Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources West Anhui UniversityLu an 237012China Key Laboratory of Biomimetic Sensor and Detecting Technology of Anhui Province West Anhui UniversityLu an 237012China Anhui Academy of Forestry Hefei 230031China Lu an Yuqing Green Agricultural Technology Co. Ltd.Lu an 237010China Department of Biological and Pharmaceutical Engineering West Anhui UniversityLu an 237012China

[Objectives]The paper was to master the species,incidence regularity and control techniques of main diseases and insect pests of Camellia oleifera in Anhui Province.[Methods]The species of main diseases and insect pests of *** in major *** afforestation bases and seedling bases in Anhui Province were investigated through field survey and literature ***,the symptom characteristics,occurrence regularity and harms of diseases and insect pests were analyzed,and scientific and reasonable control techniques were put forward.[Results]The main diseases of *** in Anhui Province were soft rot disease,blister blight,anthracnose,sooty blotch,etc.,and the main insect pests were Euproctis pseudoconspersa,Biston marginata,Hypomeces squamosus,Curculio chinensts,Chrenoma atritarsis,*** control techniques mainly included ecological regulation,physical prevention and control,chemical prevention and control,and biological prevention and control.[Conclusions]The results will promote the high-quality development of *** industry in Anhui Province,and contribute to the improvement of China s edible vegetable oil supply and national grain and oil security.

关键词： Anhui Province Camellia oleifera Diseases Insect pests Prevention and control technique