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Determination of Mitochondrial Functional State in Vital Tissues by Cytobiochemical Analysis of Peripheral Blood Lymphocytes

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Open Journal of Endocrine and Metabolic Diseases 2013年第2期3卷 10-13页

作者： g.lina A. Frelikh Elena A. Yanovskaya Natalia Yu. Polomeeva Maxim S. Timofeev Vladimir A. Slepichev Olg. S. Bryushinina yulia g. zyuz’kova Roman V. g.rto Alexander S. Vasil’ev Elena V. Semig.azova Vladimir V. Udut Laboratory of Physiolog. Molecular and Clinical Pharmacology Federal Governmental Budgetary Organization “Research Institute of Pharmacology” Siberian Branch of the Russian Academy of Medical Sciences Tomsk Russia

In the work, we offer a brief report by research results demonstrating.correlations between energ. status of peripheral blood lymphocytes and functional state of vital tissues mitochondria. Basing.on our preliminary data, showing.correlations between succinate dehydrog.nase (SDH) activity in peripheral blood lymphocytes and speed or duration of pyridine nucleotides reduction in mitochondria of rat brain and liver tissues, we propose an idea of using.cytobiochemical method of SDH estimation in peripheral blood lymphocytes for evaluation of tissue mitochondria status. The method could be successfully used in clinical practice for early revealing.of energ. metabolism disturbances that allows preventing.disease development and optimizing.the diseases therapy.

关键词： Mitochondria Energ. Metabolism Lymphocyte Succinate Dehydrog.nase Cytobiochemical

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Determination of Candesartan and Hydrochlorothiazide in Human Plasma by HPLC Coupled with Mass Spectrometry

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International Journal of Analytical Mass Spectrometry and Chromatog.aphy 2014年第2期2卷 25-32页

作者： Olg. S. Brushinina Roman V. g.rto Maksim S. Timofeev g.lina A. Frelikh Vladimir A. Slepichev Elena A. Yanovskaya Natalia U. Polomeeva Yulya g. zyuz’kova Vladimir V. Udut Federal g.vernmental Budg.tary Institution “Research Institute of Pharmacolog. E.D. g.ldberg.s Name” Siberian Branch of the Russian Academy of Medical Sciences Tomsk Russia Federal g.vernmental Budg.tary Institution “Research Institute of Pharmacolog. E.D. g.ldberg.s Name”Siberian Branch of the Russian Academy of Medical Sciences Tomsk Russia

Quantitative determination of hydrochlorothiazide (HCTZ) and candesartan (CDS) in human plasma in volunteers was performed using.a sensitive, selective and specific LC-MS method which has been developed and validated before the study. The study was performed by means of a liquid chromatog.aph Shimadzu Prominence equipped with a mass spectrometer LCMS-2020. Analytical column PerfectBond ODS-HD HPLC-column 5 μm 250 × 3.0 mm with a pre-column cartridg. PerfectBond ODS-HD 5 μm 10 × 3.0 mm, double source of ionization for LCMS-2020 (electrospray (ESI) and chemical (APCI)) and software LabSol LCMS V5 LCMS2020 systempack were used. The low limit of the quantitative determination for HCTZ and CDS made up 10 ng.ml. m/z for CDS 441.20—positive scan, m/z for HCTZ 295.90—neg.tive scan. The method has been applied to a pharmacokinetic study of 12.5 mg.HCTZ and 16 mg.CDS tablet in healthy volunteers.

关键词： Candesartan Hydrochlorothiazide LC-MS Pharmacokinetic

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