Determination of Candesartan and Hydrochlorothiazide in Human Plasma by HPLC Coupled with Mass Spectrometry
Determination of Candesartan and Hydrochlorothiazide in Human Plasma by HPLC Coupled with Mass Spectrometry作者机构：Federal Governmental Budgetary Institution “Research Institute of Pharmacology E.D. Goldberg’s Name” Siberian Branch of the Russian Academy of Medical Sciences Tomsk Russia Federal Governmental Budgetary Institution “Research Institute of Pharmacology E.D. Goldberg’s Name”Siberian Branch of the Russian Academy of Medical Sciences Tomsk Russia
出 版 物：《International Journal of Analytical Mass Spectrometry and Chromatography》 (分析质谱与色谱期刊（英文）)
年 卷 期：2014年第2卷第2期
页 面：25-32页
主 题：Candesartan Hydrochlorothiazide LC-MS Pharmacokinetic
摘 要：Quantitative determination of hydrochlorothiazide (HCTZ) and candesartan (CDS) in human plasma in volunteers was performed using a sensitive, selective and specific LC-MS method which has been developed and validated before the study. The study was performed by means of a liquid chromatograph Shimadzu Prominence equipped with a mass spectrometer LCMS-2020. Analytical column PerfectBond ODS-HD HPLC-column 5 μm 250 × 3.0 mm with a pre-column cartridge PerfectBond ODS-HD 5 μm 10 × 3.0 mm, double source of ionization for LCMS-2020 (electrospray (ESI) and chemical (APCI)) and software LabSol LCMS V5 LCMS2020 systempack were used. The low limit of the quantitative determination for HCTZ and CDS made up 10 ng/ml. m/z for CDS 441.20—positive scan, m/z for HCTZ 295.90—negative scan. The method has been applied to a pharmacokinetic study of 12.5 mg HCTZ and 16 mg CDS tablet in healthy volunteers.
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