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Detection and evaluation of different morphological forms of Mycoplasma hominis in human semen

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Asian Journal of Andrology 2024年第1期26卷 71-76页

作者： Elizaveta Bragina Tatyana Sorokina Avtandil Chogovadze yulia Shevchenko vyacheslav Chernykh Olga Barkhatova Irina Rakovskaya Research Centre for Medical Genetics Moscow 115522Russia A.N.Belozersky Institute of Physico-Chemical Biology Lomonosov Moscow State University Moscow 119992Russia Reprolab Limited Liability Company Moscow 119333Russia N.I.Pirogov Russian National Research Medical University Moscow 117997Russia Federal Government Budgetary Institution"National Research Center for Epidemiology and Microbiology Named after Honorary Academician N.F.Gamaleya of the Ministry of Health of the Russian Federation" Moscow 123098Russia

Recently discovered microcolonial forms of Mycoplasma hominis(***)and their impact on human spermatogenesis are *** spermatozoa of 125 fertile men(sperm donors;from Reprobank[Reproductive Tissue Bank,Moscow,Russial)and of 93 patients with fertility problems(from the Federal State Budgetary Institution"Research Centre for Medical Genetics[RCMG]",Moscow,Russia)were *** colonies of *** and microcolonies were detected by molecular biological methods,culture of bacteria,and transmission electron *** unique structure of microcolonial cells,in which the cytoplasmic cylinder is surrounded by concentric electron-dense and electron-light layers with a periodicity of 12-14 nm,and the ability of microcolonial cells to attach to spermatozoa are *** patients with lower sperm quality,microcolonies of *** were detected 2.5 times more frequently than classical *** detection of microcolonies in the ejaculate and the frequent isolation of microcolonies from sperm samples of patients with fertility problems suggest that microcolonial cells may be one cause of infertility.

关键词： ejaculate fertility Mycoplasma hominis spermatogenesis transmission electron microscopy

Extracellular vesicles-coupled miRNAs from oviduct and uterus modulate signaling pathways related to lipid metabolism and bovine early embryo development

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1528-1549页

作者： Rosane Mazzarella Karina Canon‑Beltran yulia N.Cajas Meriem Hamdi Encina M.Gonzalez Juliano C.da Silveira Claudia L.v.Leal D.Rizos Department of Animal Reproduction INIA-CSICMadridSpain Department of Biochemistry and Molecular Biology Veterinary FacultyComplutense University of Madrid(UCM)MadridSpain Department Agrarian Production Technical University of MadridUPMMadridSpain Departamento de Ciencias Biologicas Universidad Tecnica Particular de LojaUTPLLojaEcuador Department of Anatomy and Embryology FV-UCMMadridSpain Department of veterinary Medicine FZEA-USPPirassunungaBrazil

Background Extracellular vesicles(Evs) present in oviductal(OF) and uterine fluid(UF) have been shown to enhance bovine embryo quality during in vitro culture by reducing lipid contents and modulating lipid metabolism-related genes(LMGs), while also influencing cell proliferation, suggesting their involvement on the regulation of different biological pathways. The regulation of signaling pathways related to cell differentiation, proliferation, and metabo-lism is crucial for early embryo development and can determine the success or failure of the pregnancy. Bioactive molecules within Evs in maternal reproductive fluids, such as micro RNAs(miRNAs), may contribute to this regulatory process as they modulate gene expression through post-transcriptional *** This study evaluated miRNA cargo in OF-Evs from the early luteal phase and UF-Evs from the mid-luteal phase, coinciding with embryo transit within oviduct and uterus in vivo, and its possible influence on LMGs and sign-aling pathways crucial for early embryo development. A total of 333 miRNAs were detected, with 11 exclusive to OF, 59 to UF, and 263 were common between both groups. From the 20 differentially expressed miRNAs, 19 up-regulated in UF-Evs(bta-miR-134, bta-miR-151-3p, bta-miR-155, bta-miR-188, bta-miR-181b, bta-miR-181d, bta-miR-224, bta-miR-23b-3p, bta-miR-24-3p, bta-miR-27a-3p, bta-miR-29a, bta-miR-324, bta-miR-326, bta-miR-345-3p, bta-miR-410, bta-miR-652, bta-miR-677, bta-miR-873 and bta-miR-708) and one(bta-miR-148b) in OF-Evs. These miRNAs were predicted to modulate several pathways such as Wnt, Hippo, MAPK, and lipid metabolism and degradation. Differ-ences in miRNAs found in OF-Evs from the early luteal phase and UF-Evs from mid-luteal phase may reflect differ-ent environments to meet the changing needs of the embryo. Additionally, miRNAs may be involved, particularly in the uterus, in the regulation of embryo lipid metabolism, immune system, and *** Our study su

关键词： Embryo-maternal interaction Epigenetic Exosomes Mammalian Preimplantation Reproductive fluids

The Combined Effect of Lumenato and Ceramide in the Protection of Collagen Damage Induced by Neutrophils in Normal Human Dermal Fibroblasts

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Advances in Bioscience and Biotechnology 2024年第2期15卷 140-159页

作者： yulia Solomonov Rachel Levy Immunology and Infectious Diseases Laboratory Department of Clinical Biochemistry and Pharmacology Faculty of Health Sciences Ben-Gurion University of The Negev Beer-Sheva Israel

Introduction: Collagen is the primary structural protein fibroblasts produce in the skin’s extracellular matrix. Infiltration of neutrophils into the epidermis and dermis by exposure to Uv causes collagen damage and contributes to photoaging. Methods: To study the combined effect of Lumenato and ceramide in preventing collagen-1 damage induced by phagocytes, we used co-cultures of normal human dermal fibroblasts (fibroblasts) and activated human neutrophils. The present study aimed to determine the protective effect of the combination of Lumenato and ceramide on fibroblast collagen-1 damage induced by neutrophils. Results: Lumenato (in the range of 6.5 - 208 μg/ml) or ceramide (in the range of 0.1 - 50 μM) inhibited the production of superoxides and MPO by TNFα-stimulated neutrophils, as well as the production of NO by LPS-stimulated macrophages in a dose-dependent manner. The combinations of Lumenato and ceramide, in low concentrations, caused synergistic prevention of fibroblasts’ collagen-1 damage induced by TNFα-activated neutrophils, detected by fluorescence immunostaining and WB analysis. MPO activity in the supernatants of the co-cultures was also synergistically inhibited. Adding Lumenato or ceramide singly or in combinations in these low concentrations to the fibroblast cultures did not affect the expression of collagen-1. The combinations of Lumenato or ceramide in these concentrations also caused a synergistic inhibition of NO production by activated macrophages. Conclusions: The results suggest that combining low concentrations of Lumenato and ceramide results in synergistic protection against fibroblasts’ collagen-1 damage induced by neutrophils, thus indicating their possible potential for enhanced skin health.

关键词： Dermal Fibroblasts Neutrophils Collagen-1 Lumenato Ceramide

Olefin Polymerization Behavior of Titanium(Iv) Complexes with Fluorinated and Non-fluorinated Aliphatic Phenoxyimine Ligands

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Chinese Journal of Polymer Science 2024年第1期42卷 52-62,I0007页

作者： vladislav A.Tuskaev Kasim F.Magomedov Svetlana Ch.Gagieva Dmitry A.Kurmaev yulia v.Nelyubina Evgenii K.Golubev Maria D.Evseeva viktor G.vasil'ev Galina G.Nikiforova Mikhail I.Buzin vyacheslav S.Bogdanov Boris M.Bulychev Department of Chemistry M.V.Lomonosov Moscow State UniversityMoscow 119992Russian Federation A.N.Nesmeyanov Institute of Organoelement Compounds Russian Academy of SciencesMoscow119991Russian Federation Enikolopov Institute of Synthetic Polymer Materials Russian Academy of SciencesMoscow 117393Russian Federation

A series of Ti(Ⅳ) dichloride and dialkoxide com plexes with phenoxyimine ligands containing fluorinated and nonfluorinated aliphatic imine fragments have been *** molecular structures of complexes 1 and 4 were established by single-crystal X-ray diffraction *** complexes adopt a distorted octahedral coordination structure around the titanium atom and two oxygen atoms are situated in trans position while two nitrogen atoms and two outgoing ligands(Cl or iPrO) are situated in cis *** of activators(MMAO-12 and combinations Et_nAICl_(3-n)+Bu_(2)Mg) and outgoing ligand(Cl or iPrO) nature on the catalytic activity and properties of the resulting polymers was *** Ti complexes,despite the nature of the outgoing ligands(Cl or iPrO) in the presence of Al/Mg activators,was found to display a high ethylene polymerization activity in the range 1600-3830 kg_(polymer)·mol_(Ti)^(-1)·h^(-1)·atm^(-1) with a viscosity average molecular weight(M_v) value in the range 1.1×10~6-7.1×10~6 Dalton(Da).The resulting UHMWPE can be processed by a solventless method into high-strength and high-modulus oriented *** rheological characteristics of a polymer melt have been *** absence of a cross-over point did not allow to compare the values of the molecular weight distribution of polymers obtained on fluorinated and non-fluorinated pre-catalysts,however,the estimation of the entanglement density is in good agreement with the mechanical characteristics of oriented film *** activation with methylalumoxane,the activity of the com plexes decreased very significantly;however,a polymer with a molecular weight of about 12 million Da was *** the process of ethylene/octene-1 copolymerization,fluorine-containing precatalysts showed a clear advantage over non-fluorinated analogues both in activity and in comonomer content.

关键词： FI catalysts Titanium(Iv) (Co)polymerization Al/Mg co-catalyst Ultra-high molecular weight polyethylene

Ataxia-telangiectasia mutated plays an important role in cerebellar integrity and functionality

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Neural Regeneration Research 2023年第3期18卷 497-502页

作者： yulia Mitiagin Ari Barzilai Department of Neurobiology the School of NeurobiologyBiophysicsMolecular Biology and BiochemistryGeorge S.WiseFaculty of Life SciencesTel Aviv UniversityTel AvivIsrael Sagol School of Neurosciences Tel Aviv UniversityTel AvivIsrael

Accumulating evidence indicates that ataxia-telangiectasia mutated kinase is critical for maintaining cellular homeostasis and that it has both nuclear and cytoplasmic ***,the functions of ataxia-telangiectasia mutated that when lost lead to cerebellar degeneration are still *** this review,we first describe the role of ataxia-telangiectasia mutated in cerebellar *** addition to its canonical nuclear functions in DNA damage response circuits,ataxia-telangiectasia mutated functions in various cytoplasmic and mitochondrial processes that are critically important for cellular *** discuss these functions with a focus on the role of ataxia-telangiectasia mutated in maintaining the homeostatic redox ***,we describe the unique functions of ataxia-telangiectasia mutated in various types of neuronal and glial cells including cerebellar granule neurons,astrocytes,and microglial cells.

关键词： ataxia telangiectasia ATM cerebellum DNA damage response double-strand breaks mitochondrial dysfunction oxidative stress single-strand breaks

Growth of Micropropagated Solanum tuberosum *** under Artificial Solar Spectrum and Different Mono-and Polychromatic LED Lights

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Horticultural Plant Journal 2022年第2期8卷 205-214页

作者： Olga v.Grishchenko Eugeny P.Subbotin Irina v.Gafitskaya yulia v.vereshchagin. Elena v.Burkovskaya yulia A.Khrolenko valeria P.Grigorchuk Olga v.Nakonechnaya victor P.Bulgakov Yuri N.Kulchin Federal Scientific Center of the East Asia Terrestrial Biodiversity Far Eastern Branch of the Russian Academy of SciencesVladivostok 690022Russia Institute of Automation and Control Processes Far Eastern Branch of the Russian Academy of SciencesVladivostok 690041Russia

In agriculture,LED light sources have increasingly replaced the standard luminescent lamps and have acquired an important role in plant *** studied the effect of different light sources such as narrow-band LEDs(bright blue,blue,green,yellow,deep red,and red)and wide-band LEDs(cold white,white,warm white,full spectrum,and an artificial solar spectrum sun box constructed by us)on development of potato plantlets in *** luminescent lamps were used as a *** light intensity of 49μmol·m^(-2)·s^(-1)was provided in all light *** showed that the long-wave narrow-band light treatments were inapplicable for potato micropropagation,because plantlets were weak with small leaves,inhibited roots,and significantly elongated *** lights provided growth of shortened plantlets with large leaves,well-growing roots,and abundant green *** chlorophyll content was lower under blue and bright blue light and was at the same level in the remained *** differences in the stomatal apparatus development were observed depending on the light *** differences were not always reflected in the plantlet phenotype:e.g.,plantlets under blue and bright blue lights showed no differences in any characteristics except stomatal density and size of stomatal guard *** found no significant effect of blue light portion in the white lights and full spectrum on plantlet *** artificial solar spectrum sun box was the most suitable for potato micropropagation,because it supported the development of plantlets with good fitness,uniform internodes length,abundant roots and green mass accumulation.

关键词： Solanum tuberosum LED Artificial solar light Growth Micropropagation

Experimental and ab initio study of Ba_(2)Na_(3)(B_(3)O_(6))_(2)F stability in the pressure range of 0–10 GPa

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International Journal of Minerals,Metallurgy and Materials 2023年第9期30卷 1846-1854页

作者： Nursultan E.Sagatov Tatyana B.Bekker yulia G.vinogradova Alexey v.Davydov Ivan v.Podborodnikov Konstantin D.Litasov Sobolev Institute of Geology and Mineralogy Siberian Branch of Russian Academy of SciencesNovosibirsk 630090Russia Department of Geology and Geophysics Novosibirsk State UniversityNovosibirsk 630090Russia Research and Development Department Novosibirsk State University of ArchitectureDesign and Arts(NSUADA)Novosibirsk 630099Russia vereshchagin Institute for High Pressure Physics Russian Academy of SciencesTroitsk 108840Russia Fersman Mineralogical Museum Russian Academy of SciencesMoscow 119071Russia

Both numerical and experimental studies of the stability and electronic properties of barium–sodium metaborate Ba_(2)Na_(3)(B_(3)O_(6))_(2)F(P63/m) at pressures up to 10 GPa have been carried out. Electronic-structure calculations with HSE06 hybrid functional showed that Ba_(2)Na_(3)(B_(3)O_(6))_(2)F has an indirect band gap of 6.289 ev. A numerical study revealed the decomposition of Ba_(2)Na_(3)(B_(3)O_(6))_(2)F into the BaB_(2)O_(4),Na BO_(2), and NaF phases above 3.4 GPa at 300 K. Subsequent high-pressure high-temperature experiments performed using ‘Discoverer-1500’DIA-type apparatus at pressures of 3 and 6 GPa and temperature of 1173 K confirmed the stability of Ba_(2)Na_(3)(B_(3)O_(6))_(2)F at 3 GPa and its decomposition into BaB_(2)O_(4), NaBO_(2), and NaF at 6 GPa, which was verified by energy-dispersive X-ray analysis and Raman spectroscopy. The observed Raman bands of the Ba_(2)Na_(3)(B_(3)O_(6))_(2)F phase were assigned by comparing the experimental and calculated spectra. The experimental Raman spectra of decomposition reaction products obtained at 6 GPa suggest the origin of a new high-pressure modification of barium metaborate BaB_(2)O_(4).

关键词： density functional theory phase stability borate high pressure Raman spectroscopy

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当女孩走上相扑赛场

中国慈善家 2024年第2期 86-97页

作者：符遥尤利娅·斯科戈列娃(摄影) 不详

019年9月,日本东京。在日本女子相扑联合会举办的全国女子相扑青少年相扑大赛上,来自俄罗斯的摄影师尤利娅·斯科戈列娃(yulia Skokoreva)第一次见到了11岁的娜娜。这是日本有史以来的第一个面向小学4-6年级女子相扑选手开放的全国性比... 详细信息

019年9月,日本东京。在日本女子相扑联合会举办的全国女子相扑青少年相扑大赛上,来自俄罗斯的摄影师尤利娅·斯科戈列娃(yulia Skokoreva)第一次见到了11岁的娜娜。这是日本有史以来的第一个面向小学4-6年级女子相扑选手开放的全国性比赛,年轻的女孩们从全国各地汇聚于此。在场的大多数观众是为孩子加油助威的父母,而娜娜拥有其中最大的“粉丝后援团”,他们高举着海报和旗帜,上面有她的名字和照片。

关键词：相扑尤利娅俄罗斯赛场青少年摄影师娜娜女孩

Quantifying social costs of coal-fired power plant generation

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Geography and Sustainability 2023年第1期4卷 39-48页

作者： Andewi Rokhmawati Agus Sugiyono yulia Efni Rendra Wasnury The Faculty of Economics and Business Universitas RiauRiau 28293Indonesia Research Center for Energy Conversion and Conservation(PRKKE) National Research and Innovation Agency(BRIN)BantenIndonesia

Coal has been dominating the electricity supply in Indonesia,especially in long-term power generation from fossil *** dominance is due to lower production costs in coal-fired power plant ***,this low price is only based on monetary costs and ignores the social ***,this study aims to quantify the social costs of coal-fired *** QUERI-AirPacts modeling,the present study quantifies the social costs resulting from the Tenayan Raya coal-fired generation in Riau,*** includes the levelized cost of electricity and health costs into the generation *** that,this study calculates the net present value,internal rate return,and project payback *** study found that as much as$50.22/MWh was the levelized cost of ***$15.978/MWh or$0.015978/kWh was the social cost that was not included in the generating *** the electricity production level of 1,380,171.69 MWh per year,there is an expected extra cost of$22,052,383.30 uncounted when externalities are *** instance,the net present value(NPv)is lower and even negative when external costs are included(-$24,062,274.19)compared to$176,108,091.52 when externalities are not *** internal rate of return(IRR)is much higher when the social costs are not *** payback period is also shorter when the social costs are excluded than when the externalities are *** global number indicates that the inclusion of external costs would impact NPv,IRR,and the payback *** result implies that the government should internalize the external cost to stimulate the electricity producers to conduct cost-benefit *** cost-benefit analysis mechanism would lead the producers to be more efficient.

关键词： AirPact Coal-fired generation Externality cost Health cost Levelized cost of electricity Social cost