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Influence of unrecorded alcohol consumption on liver cirrhosis mortality

World Journal of Gastroenterology 2014年第23期20卷 7217-7222页

作者： Dirk W Lachenmeier yulia b monakhova Jürgen Rehm Epidemiological Research Unit Institute for Clinical Psychology and PsychotherapyTechnische Universit?t Dresden01187 DresdenGermany Chemisches und Veterin?runtersuchungsamt (CVUA) Karlsruhe 76187 KarlsruheGermany Centre for Addiction and Mental Health (CAMH) TorontoON M5S 2S1Canada Dalla Lana School of Public Health University of TorontoTorontoON M5T 3M7Canada

Unrecorded alcohol includes illegally distributed alcohol as well as homemade or surrogate alcohol which is unintended for consumption by humans(e.g.,cosmetics containing alcohol).The highest unrecorded alcohol consumption occurs in Eastern Europe and some of these countries have an over proportional liver cirrhosis *** besides ethanol have been hypothesized as being responsible for this *** the other hand,chemical investigations were unable to prove that unrecorded alcohol regularly contains contaminants above toxicological ***,illegally produced spirits regularly contain higher percentages of alcohol(above 45%by volume),but for considerably less costs compared with licit beverages,potentially causing more problematic patterns of *** this review,it is investigated whether patterns of drinking rather than product composition can explain the liver cirrhosis mortality *** examination of World Health Organization country data shows that the originally detected correlation of the percentage of unrecorded alcohol consumption and liver cirrhosis mortality rates disappears when the data is adjusted for the prevalence of heavy episodic *** may be concluded that there is currently a lack of data to demonstrate causality between the composition of illicit spirits(e.g.,higher levels of certain contaminants in home-produced products)and liver toxicity on a population *** may be cases of poisoning with antiseptic liquids containing compounds such as polyhexamethyleneguanidine,which were reported to be consumed as surrogate alcohol in Russia,leading to an outbreak of acute cholestatic liver injury,histologically different from conventional alcoholic liver disease.

关键词： Epidemiology Liver cirrhosis Alcoholic beverages Unrecorded alcohol Risk assessment

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Extracellular vesicles-coupled miRNAs from oviduct and uterus modulate signaling pathways related to lipid metabolism and bovine early embryo development

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Journal of Animal Science and biotechnology 2024年第4期15卷 1528-1549页

作者： Rosane Mazzarella Karina Canon‑beltran yulia N.Cajas Meriem Hamdi Encina M.Gonzalez Juliano C.da Silveira Claudia L.V.Leal D.Rizos Department of Animal Reproduction INIA-CSICMadridSpain Department of biochemistry and Molecular biology Veterinary FacultyComplutense University of Madrid(UCM)MadridSpain Department Agrarian Production Technical University of MadridUPMMadridSpain Departamento de Ciencias biologicas Universidad Tecnica Particular de LojaUTPLLojaEcuador Department of Anatomy and Embryology FV-UCMMadridSpain Department of Veterinary Medicine FZEA-USPPirassunungaBrazil

background Extracellular vesicles(EVs) present in oviductal(OF) and uterine fluid(UF) have been shown to enhance bovine embryo quality during in vitro culture by reducing lipid contents and modulating lipid metabolism-related genes(LMGs), while also influencing cell proliferation, suggesting their involvement on the regulation of different biological pathways. The regulation of signaling pathways related to cell differentiation, proliferation, and metabo-lism is crucial for early embryo development and can determine the success or failure of the pregnancy. bioactive molecules within EVs in maternal reproductive fluids, such as micro RNAs(miRNAs), may contribute to this regulatory process as they modulate gene expression through post-transcriptional *** This study evaluated miRNA cargo in OF-EVs from the early luteal phase and UF-EVs from the mid-luteal phase, coinciding with embryo transit within oviduct and uterus in vivo, and its possible influence on LMGs and sign-aling pathways crucial for early embryo development. A total of 333 miRNAs were detected, with 11 exclusive to OF, 59 to UF, and 263 were common between both groups. From the 20 differentially expressed miRNAs, 19 up-regulated in UF-EVs(bta-miR-134, bta-miR-151-3p, bta-miR-155, bta-miR-188, bta-miR-181b, bta-miR-181d, bta-miR-224, bta-miR-23b-3p, bta-miR-24-3p, bta-miR-27a-3p, bta-miR-29a, bta-miR-324, bta-miR-326, bta-miR-345-3p, bta-miR-410, bta-miR-652, bta-miR-677, bta-miR-873 and bta-miR-708) and one(bta-miR-148b) in OF-EVs. These miRNAs were predicted to modulate several pathways such as Wnt, Hippo, MAPK, and lipid metabolism and degradation. Differ-ences in miRNAs found in OF-EVs from the early luteal phase and UF-EVs from mid-luteal phase may reflect differ-ent environments to meet the changing needs of the embryo. Additionally, miRNAs may be involved, particularly in the uterus, in the regulation of embryo lipid metabolism, immune system, and *** Our study su

关键词： Embryo-maternal interaction Epigenetic Exosomes Mammalian Preimplantation Reproductive fluids

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Unraveling the mark of surface defects on a spinterface： The nitronyl nitroxide/TiO2（110） interface

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Nano Research 2016年第11期9卷 3515-3527页

作者： Reza Kakavandi Arrigo Calzolari yulia b. borozdina Prince Ravat Thomas Chasse Martin baumgarten M. benedetta Casu Institute of Physical and Theoretical Chemistry University of Tubingen Auf der Morgenstelle 18 72076 Tubingen Germany CNR-NANO Istituto Nanoscienze Centro S3 41125 Modena Italy Max Planck Institute for Polymer Research Ackermannweg 10 55128 Mainz Germany

Metal-free organic radicals are fascinating materials owing to their unique properties. Having a stable magnetic moment coupled to light elements makes these materials central to develop a large variety of applications. We investigated the magnetic spinterface coupling between the surface of a single rutile TiO2（110） crystal and a pyrene-based nitronyl nitroxide radical, using a combination of thickness-dependent X-ray photoelectron spectroscopy and ab initio calculations. The radicals were physisorbed, and their magnetic character was preserved on the （almost） ideal surface. The situation changed completely when the molecules interacted with a surface defect site upon adsorption. In this case, the reactivity of the defect site led to the quenching of the molecular magnetic moment. Our work elucidates the crucial role played by the surface defects and demonstrates that photoemission spectroscopy combined with density functional theory calculations can be used to shed light on the mechanisms governing complex interfaces, such as those between magnetic molecules and metal oxides.

关键词： organic spinterface photoemission spectroscop density functional theory calculations

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MicroRNA Regulation in Extreme Environments: Differential Expression of MicroRNAs in the Intertidal Snail Littorina littorea During Extended Periods of Freezing and Anoxia

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Genomics, Proteomics & bioinformatics 2012年第5期10卷 302-309页

作者： Kyle K.biggar Samantha F.Kornfeld yulia Maistrovski Kenneth b.Storey Institute of biochemistry&Department of biology Carleton University

Several recent studies of vertebrate adaptation to environmental stress have suggested roles for microRNAs （miRNAs） in regulating glo- bal suppression of protein synthesis and/or restructuring protein expression patterns. The present study is the first to characterize stress- responsive alterations in the expression of miRNAs during natural freezing or anoxia exposures in an invertebrate species, the intertidal gastropod Littorina littorea. These snails are exposed to anoxia and freezing conditions as their environment constantly fluctuates on both a tidal and seasonal basis. The expression of selected miRNAs that are known to influence the cell cycle, cellular signaling pathways, carbohydrate metabolism and apoptosis was evaluated using RT-PCR. Compared to controls, significant changes in expression were observed for miR-la-1, miR-34a and miR-29b in hepatopancreas and for miR-la-1, miR-34a, miR-133a, miR-125b, miR-29b and miR-2a in foot muscle after freezing exposure at -6 ~C for 24 h （P 〈 0.05）. In addition, in response to anoxia stress for 24 h, significant changes in expression were also observed for miR-la-1, miR-210 and miR-29b in hepatopancreas and for miR-1 a-1, miR-34a, miR-133a, miR-29b and miR-2a in foot muscle （P 〈 0.05）. Moreover, protein expression of Dicer, an enzyme responsible for mature microRNA processing, was increased in foot muscle during freezing and anoxia and in hepatopancreas during freezing. Alterations in expression of these miRNAs in L. littorea tissues may contribute to organismal survival under freezing and anoxia.

关键词： Freeze tolerance Anaerobiosis Metabolic rate depression Periwinkle Marine mollusk

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Experimental and ab initio study of ba_(2)Na_(3)(b_(3)O_(6))_(2)F stability in the pressure range of 0–10 GPa

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International Journal of Minerals,Metallurgy and Materials 2023年第9期30卷 1846-1854页

作者： Nursultan E.Sagatov Tatyana b.bekker yulia G.Vinogradova Alexey V.Davydov Ivan V.Podborodnikov Konstantin D.Litasov Sobolev Institute of Geology and Mineralogy Siberian Branch of Russian Academy of SciencesNovosibirsk 630090Russia Department of Geology and Geophysics Novosibirsk State UniversityNovosibirsk 630090Russia Research and Development Department Novosibirsk State University of ArchitectureDesign and Arts(NSUADA)Novosibirsk 630099Russia Vereshchagin Institute for High Pressure Physics Russian Academy of SciencesTroitsk 108840Russia Fersman Mineralogical Museum Russian Academy of SciencesMoscow 119071Russia

both numerical and experimental studies of the stability and electronic properties of barium–sodium metaborate ba_(2)Na_(3)(b_(3)O_(6))_(2)F(P63/m) at pressures up to 10 GPa have been carried out. Electronic-structure calculations with HSE06 hybrid functional showed that ba_(2)Na_(3)(b_(3)O_(6))_(2)F has an indirect band gap of 6.289 eV. A numerical study revealed the decomposition of ba_(2)Na_(3)(b_(3)O_(6))_(2)F into the bab_(2)O_(4),Na bO_(2), and NaF phases above 3.4 GPa at 300 K. Subsequent high-pressure high-temperature experiments performed using ‘Discoverer-1500’DIA-type apparatus at pressures of 3 and 6 GPa and temperature of 1173 K confirmed the stability of ba_(2)Na_(3)(b_(3)O_(6))_(2)F at 3 GPa and its decomposition into bab_(2)O_(4), NabO_(2), and NaF at 6 GPa, which was verified by energy-dispersive X-ray analysis and Raman spectroscopy. The observed Raman bands of the ba_(2)Na_(3)(b_(3)O_(6))_(2)F phase were assigned by comparing the experimental and calculated spectra. The experimental Raman spectra of decomposition reaction products obtained at 6 GPa suggest the origin of a new high-pressure modification of barium metaborate bab_(2)O_(4).

关键词： density functional theory phase stability borate high pressure Raman spectroscopy

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The CELLULOSE-SYNTHASE LIKE C （CSLC） Family of barley Includes Members that Are Integral Membrane Proteins Targeted to the Plasma Membrane

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Molecular Plant 2009年第5期2卷 1025-1039页

作者： Fenny M. Dwivany Dina yulia Rachel A, burton Neil J. Shirley Sarah M. Wilson Geoffrey b, Fincher Antony bacic Ed Newbigin Monika S. Doblin Plant Cell biology Research Centre School of Botany. University of Melbourne Victoria 3010 Australia Australian Centre for Plant Functional Genomics School of Agriculture and Wine University of Adelaide South Australia 5064 Australia Australian Centre for Plant Functional Genomics School of Botany University of Melbourne Victoria 3010 Australia Present address: Department of biology Institut Teknologi Bandung Bandung Indonesia Present address: Stem Cell and Cancer Institute Jh Jend. Ahmad Yani No.2 Pulo Mas Jakarta 13210 Indonesia

The CELLULOSE SYNTHASE-LIKE C （CSLC） family is an ancient lineage within the CELLULOSE SYNTHASE/CELLULOSE SYNTHASE-LIKE （CESA/CSL） polysaccharide synthase superfamily that is thought to have arisen before the divergence of mosses and vascular plants. As studies in the flowering plant Arabidopsis have suggested synthesis of the （1,4）-β-glucan backbone of xyloglucan （XyG）, a wall polysaccharide that tethers adjacent cellulose microfibrils to each other, as a probable function for the CSLCs, CSLC function was investigated in barley （Hordeum vulgate L.）, a species with low amounts of XyG in its walls. Four barley CSLCgenes were identified （designated HvCSLC1-4）. Phylogenetic analysis reveals three well supported clades of CSLCs in flowering plants, with barley having representatives in two of these clades. The four barley CSLCs were expressed in various tissues, with in situ PCR detecting transcripts in all cell types of the coleoptile and root, including cells with primary and secondary cell walls. Co-expression analysis showed that HvCSLC3 was coordinately expressed with putative XyG xylosyltransferase genes. both immuno-EM and membrane fractionation showed that HvCSLC2 was located in the plasma membrane of barley suspension-cultured cells and was not in internal membranes such as endoplasmic reticulum or Golgi apparatus. based on our current knowledge of the sub-cellular locations of polysaccharide synthesis, we conclude that the CSLC family probably contains more than one type of polysaccharide synthase.

关键词： Cellulose synthase-like family C plant cell wall biosynthesis xyloglucan cellulose glycosyltransferase

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An Update on Abscisic Acid Signaling in Plants and More

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Molecular Plant 2008年第2期1卷 198-217页

作者： Aleksandra Wasilewsk.a Florina Vlad Caroline Sirichandra yulia Redko Fabien Jammes Christiane Valon Nicolas Frei dit Frey Jeffrey Leung Institut des Sciences du Vegetal Centre National de la Recherche Scientifique UPR 23551 Avenue de la Terrasse Bat. 23 91190 Gif-sur-Yvette France CNRS UPR9073 Institut de Biologie Physico-Chimique 13 rue Pierre et Marie Curie 75005 Paris France Laboratoire Signalisation et Regulation Coordonnee du Metabolisme Carbone et Azote Institut de Biotechnologie des Plantes (UMR8618) Universite Paris-Sud F-91405 Orsay Cedex France

The mode of abscisic acid （AbA） action, and its relations to drought adaptive responses in particular, has been a captivating area of plant hormone research for much over a decade. The hormone triggers stomatal closure to limit water loss through transpiration, as well as mobilizes a battery of genes that presumably serve to protect the cells from the ensuing oxidative damage in prolonged stress. The signaling network orchestrating these various responses is, however, highly complex. This review summarizes several significant advances made within the last few years. The biosynthetic pathway of the hormone is now almost completely elucidated, with the latest identification of the AbA4 gene encoding a neoxanthin synthase, which seems essential for de novo AbA biosynthesis during water stress. This leads to the interesting question on how AbA is then delivered to perception sites. In this respect, regulated transport has attracted renewed focus by the unexpected finding of a shoot-to-root translocation of AbA during drought response, and at the cellular level, by the identification of a b-galactosidase that releases biologically active AbA from inactive AbA-glucose ester. Surprising candidate AbA receptors were also identified in the form of the Flowering Time Control Protein A （FCA） and the Chloroplastic Magnesium Protoporphyrin-IX Chelatase H subunit （CHLH） in chloroplast-nucleus communication, both of which have been shown to bind AbA in vitro. On the other hand, the protein（s） corresponding to the physiologically detectable cell-surface AbA receptor（s） is （are） still not known with certainty. Genetic and physiological studies based on the guard cell have reinforced the central importance of reversible phosphorylation in modulating rapid AbA responses. Sucrose Non-Fermenting Related Kinases （SnRK）, Calcium-Dependent Protein Kinases （CDPK）, Protein Phosphatases （PP） of the 2C and 2A classes figure as prominent regulators in this single-cell model. Identifying their d

关键词： Abscisic Acid Signaling Plants and More

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MicroRNAs as a potential prognostic factor in gastric cancer

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World Journal of Gastroenterology 2011年第35期17卷 3976-3985页

作者： baruch brenner Moshe b Hoshen Ofer Purim Miriam ben David Karin Ashkenazi Gideon Marshak yulia Kundel Ronen brenner Sara Morgenstern Marisa Halpern Nitzan Rosenfeld Ayelet Chajut Yaron Niv Michal Kushnir Institute of Oncology Davidoff CenterRabin Medical CenterBeilinson HospitalPetach Tikva 49100Israel Sackler Faculty of Medicine Tel Aviv UniversityTel Aviv 69978Israel Rosetta Genomics Ltd. Rehovot 76706Israel Clalit Research Institute Clalit Medical ServicesTel Aviv 69978Israel Institute of Oncology Rabin Medical CenterGolda-Hasharon HospitalPetach Tikva 49100Israel Institute of Oncology Rabin Medical CenterBeilinson HospitalPetach Tikva 49100Israel Institute of Pathology Rabin Medical CenterBeilinson HospitalPetach Tikva 49100Israel Institute of Pathology Rabin Medical CenterGolda-Hasharon HospitalPetach Tikva 49100Israel Cancer Research UK Cambridge Research InstituteCambridge CB2 OREUnited Kingdom Department of Gastroenterology Rabin Medical CenterBeilinson HospitalPetach Tikva 49100Israel Sackler Faculty of Medicine Tel Aviv UniversityTel Aviv 69978 Israel

AIM:To compare the microRNA (miR) profiles in the primary tumor of patients with recurrent and non-recurrent gastric ***:The study group included 45 patients who underwent curative gastrectomies from 1995 to 2005 without adjuvant or neoadjuvant therapy and for whom adequate tumor content was *** RNA was extracted from formalin-fixed paraffin-embedded tumor samples,preserving the small RNA *** profiling using miR microarrays was performed to identify potential biomarkers of recurrence after *** expression of the differential miRs was later verified by quantitative real-time polymerase chain reaction (qRT-PCR).Findings were compared between patients who had a recurrence within 36 mo of surgery (bad-prognosis group,n=14,31%) and those who did not (good-prognosis group,n=31,69%).RESULTS:Three miRs,miR-451,miR-199a-3p and miR-195 were found to be differentially expressed in tumors from patients with good prognosis vs patients with bad prognosis (Pboth recurrence and *** miR-451,the positive predictive value for non-recurrence was 100% (13/13).The expression of the differential miRs was verified by qRT-PCR,showing high correlation to the microarray data and similar separation into prognosis ***:This study identified three miRs,miR-451,miR-199a-3p and miR-195 to be predictive of recurrence of gastric *** these,miR-451 had the strongest prognostic impact.

关键词： MicroRNA Prognosis Recurrence Gastric cancer

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Formation of Extended Covalently bonded Ni Porphyrin Networks on the Au（lll） Surface

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Nano Research 2011年第4期4卷 376-384页

作者： Sergey A. Krasnikov Catherine M. Doyle Natalia N. Sergeeva Alexei b. Preobrajenski Nikolay A.Vinogradov yulia N. Sergeeva Alexei A. Zakharov Mathias O. Senge Attilio A. Cafolla School of Physical Sciences Dublin City University Glasnevin Dublin 9 Ireland Centre tbr Research on Adaptive Nanostructures and Nanodevices （CRANN） School of Physics Trinity College Dublin Dublin 2 Ireland SFI Tetrapyrrole Laboratory School of Chemistry Trinity College Dublin Dublin 2 Ireland MAX-Lab Lund University Box 118 22100 Lund Sweden

The growth and ordering of {5,10,15,20-tetrakis（4-bromophenyl）porphyrinato}nickel（II）（NiTbrPP） molecules on the Au（111） surface have been investigated using scanning tunnelling microscopy, X-ray absorption, core-level photoemission, and microbeam low-energy electron diffraction. When deposited onto the substrate at room temperature, the NiTbrPP forms a well-ordered close-packed molecular layer in which the molecules have a flat orientation with the porphyrin macrocycle plane lying parallel to the substrate. Annealing of the NiTbrPP layer on the Au（111） surface at 525 K leads to dissociation of bromine from the porphyrin followed by the formation of covalent bonds between the phenyl substituents of the porphyrin. This results in the formation of continuous covalently bonded porphyrin networks, which are stable up to 800 K and can be recovered after exposure to ambient conditions. by controlling the experimental conditions, a robust, extended porphyrin network can be prepared on the Au（111） surface that has many potential applications such as protective coatings, in sensing or as a host structure for molecules and clusters.

关键词： Porphyrins covalently bonded networks scanning tunnelling microscopy X-ray photoemission spectroscopy near-edge X-ray absorption fine structure Au(111)

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Case series in Indonesia:b.1.617.2(delta)variant of SARS-CoV-2 infection after a second dose of vaccine

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World Journal of Clinical Cases 2022年第36期10卷 13216-13226页

作者： Anis Karuniawati Ari F Syam Armand Achmadsyah Fera Ibrahim yulia Rosa Pratiwi Sudarmono Fadilah Fadilah Menaldi Rasmin Department of Clinical Microbiology Faculty of MedicineUniversitas Indonesia/Cipto Mangunkusumo Jakarta IndonesiaJakarta 10430DKI JayaIndonesia Division of Gastroenterology Department of Internal MedicineFaculty of MedicineUniversitas Indonesia/Cipto Mangunkusumo Jakarta IndonesiaJakarta PUsat 10430DKI JayaIndonesia Faculty of Medicine Universits IndonesiaJakarta Pusat 10430DKI JayaIndonesia Department of Medical Chemistry Faculty of MedicineUniversitas IndonesiaJakarta IndonesiaFaculty of MedicineUniversitas IndonesiaJakarta 10430DKI JayaIndonesia Department of Pulmonology and Respiratory Medicine Faculty of MedicineUniversitas Indonesia/Persahabatan HospitalJakarta IndonesiaJakarta Pusat 10430DKI JayaIndonesia

bACKGROUND The b.1.617.2(delta)variant of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)was first discovered in Maharashtra in late 2020 and has rapidly expanded across India and *** took only 2 mo for this variant to spread in Indonesia,making the country the new epicenter of the delta variant as of July *** efforts made by accelerating massive rollouts of current vaccines to protect against infection,cases of fully-vaccinated people infected with the delta variant have been *** To describe the demographic statistics and clinical presentation of the delta variant infection after the second dose of vaccine in *** A retrospective,single-centre case series of the general consecutive population that worked or studied at Faculty of Medicine,Universitas Indonesia with confirmed Delta Variant Infection after a second dose of vaccine from 24 June and 25 June *** were collected retrospectively based on a combination of author recall,reverse transcription-polymerase chain reaction(RT-PCR),and whole genome sequencing results from the Clinical Microbiology Laboratory,Faculty of Medicine,Universitas *** between 24 June and 25 June 2021,15 subjects were confirmed with the b.1.617.2(delta)variant infection after a second dose of the *** subjects were vaccinated with CoronaVac(Sinovac)and one subject with ChAdOx1 nCoV-19(Oxford-AstraZeneca).All of the subjects remained in home isolation,with fever being the most common symptom at the onset of illness(n=10,66.67%).The mean duration of symptoms was 7.73 d(±5.444).The mean time that elapsed from the first positive swab to a negative RT-PCR test for SARS-CoV-2 was 17.93 d(±6.3464).The median time that elapsed from the second dose of vaccine to the first positive swab was 87 d(interquartile range:86-128).CONCLUSION Although this case shows that after two doses of vaccine,subjects are still susceptible to the delta variant infection,currently availab

关键词： COVID-19/SARS-CoV-2 infection b.1.617.2(delta)variant Fully vaccinated Case series

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