MicroRNAs as a potential prognostic factor in gastric cancer

作     者：Baruch Brenner Moshe B Hoshen Ofer Purim Miriam Ben David Karin Ashkenazi Gideon Marshak Yulia Kundel Ronen Brenner Sara Morgenstern Marisa Halpern Nitzan Rosenfeld Ayelet Chajut Yaron Niv Michal Kushnir 

作者机构：Institute of OncologyDavidoff CenterRabin Medical CenterBeilinson HospitalPetach Tikva 49100Israel Sackler Faculty of MedicineTel Aviv UniversityTel Aviv 69978Israel Rosetta Genomics Ltd.Rehovot 76706Israel Clalit Research InstituteClalit Medical ServicesTel Aviv 69978Israel Institute of OncologyRabin Medical CenterGolda-Hasharon HospitalPetach Tikva 49100Israel Institute of OncologyRabin Medical CenterBeilinson HospitalPetach Tikva 49100Israel Institute of PathologyRabin Medical CenterBeilinson HospitalPetach Tikva 49100Israel Institute of PathologyRabin Medical CenterGolda-Hasharon HospitalPetach Tikva 49100Israel Cancer Research UKCambridge Research InstituteCambridge CB2 OREUnited Kingdom Department of GastroenterologyRabin Medical CenterBeilinson HospitalPetach Tikva 49100Israel Sackler Faculty of MedicineTel Aviv UniversityTel Aviv 69978 Israel 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2011年第17卷第35期

页      面：3976-3985页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：MicroRNA Prognosis Recurrence Gastric cancer 

摘      要：AIM:To compare the microRNA (miR) profiles in the primary tumor of patients with recurrent and non-recurrent gastric ***:The study group included 45 patients who underwent curative gastrectomies from 1995 to 2005 without adjuvant or neoadjuvant therapy and for whom adequate tumor content was *** RNA was extracted from formalin-fixed paraffin-embedded tumor samples,preserving the small RNA *** profiling using miR microarrays was performed to identify potential biomarkers of recurrence after *** expression of the differential miRs was later verified by quantitative real-time polymerase chain reaction (qRT-PCR).Findings were compared between patients who had a recurrence within 36 mo of surgery (bad-prognosis group,n=14,31%) and those who did not (good-prognosis group,n=31,69%).RESULTS:Three miRs,miR-451,miR-199a-3p and miR-195 were found to be differentially expressed in tumors from patients with good prognosis vs patients with bad prognosis (P0.0002,0.0027 and 0.0046 respectively).High expression of each miR was associated with poorer prognosis for both recurrence and *** miR-451,the positive predictive value for non-recurrence was 100% (13/13).The expression of the differential miRs was verified by qRT-PCR,showing high correlation to the microarray data and similar separation into prognosis ***:This study identified three miRs,miR-451,miR-199a-3p and miR-195 to be predictive of recurrence of gastric *** these,miR-451 had the strongest prognostic impact.