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Recent advances in genome-scale engineering in Escherichia coli and their applications

Engineering Microbiology 2024年第1期4卷 28-38页

作者： Hui Gao Zhichao Qiu Xuan Wang Xiyuan zhang yujia zhang Junbiao Dai Zhuobin Liang Institute of Molecular Physiology Shenzhen Bay LaboratoryShenzhen 518132China CAS Key Laboratory of Quantitative Engineering Biology Guangdong Provincial Key Laboratory of Synthetic Genomics and Shenzhen Key Laboratory of Synthetic Genomics.Shenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Regenerative Medicine Program Bellvitge Institute for Biomedical Research(IDIBELL)and Program for Clinical Translation of Regenerative Medicine in Catalonia(P-CMRC)L’Hospitalet de LlobregatBarcelona 08908Spain Faculty of Pharmacy and Food Science Barcelona UniversityBarcelona 08028Spain College of Life Sciences Northwest A&F UniversityShaanxi 712100China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China

Owing to the rapid advancement of genome engineering technologies,the scale of genome engineering has expanded *** editing has progressed from one genomic alteration at a time that could only be employed in few species,to the simultaneous generation of multiple modifications across many genomic loci in numerous *** development and recent advances in multiplex automated genome engineering(MAGE)-associated technologies and clustered regularly interspaced short palindromic repeats and their associated protein(CRISPR-Cas)-based approaches,together with genome-scale synthesis technologies offer unprecedented opportunities for advancing genome-scale engineering in a broader *** approaches provide new tools to generate strains with desired phenotypes,understand the complexity of biological systems,and directly evolve a genome with novel ***,we review the recent major advances in genome-scale engineering tools developed for Escherichia coli,focusing on their applications in identifying essential genes,genome reduction,recoding,and beyond.

关键词： Genome-scale engineering E.coli Recombineering CRISPR-Cas

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Transforming waste particles into valuable adsorbents via amyloid-mediated molecular engineering

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Science China Chemistry 2024年

作者： Qingmin Yang Jian Zhao yujia zhang Xingyu Zhou Hao Ren Bowen Hu Zhongli Lei Lixin Chen Peng Yang School of Material and Chemical Engineering Bengbu University Anhui Provincial Engineering Laboratory of Silicon-Based Materials School of Chemistry and Chemical Engineering Northwestern Polytechnical University Key Laboratory of Applied Surface and Colloid Chemistry Ministry of Education School of Chemistry and Chemical EngineeringShaanxi Normal University Xi'an Key Laboratory of Polymeric Soft Matter School of Chemistry and Chemical Engineering Shaanxi Normal University International Joint Research Center on Functional Fiber and Soft Smart Textile School of Chemistry and Chemical EngineeringShaanxi Normal University

The high-value utilization of industrial solid waste using a facile and eco-friendly process is of great interest and significance in reducing environmental pollution and developing a green circular economy. Herein, we propose an amyloid-mediated molecular engineering strategy to transform particulate waste into valuable adsorbents for metal ions. Our method has the advantage of aqueous solution fabrication under mild conditions without the use of high-temperature hydrothermal methods and toxic chemical reagents. Amyloid-mediated molecular engineering manipulates the phase transition of bovine serum albumin（BSA）on particulate waste surfaces, resulting in a remarkable ～3.1 times improvement in the adsorption capacity of fly ash, a typical industrial solid waste for gold ions after modification with the phase-transitioned BSA（PTB）. The resultant adsorption ability was 69–1,980 times higher than those of conventional and emerging adsorbent materials such as ion exchange resins, activated carbon（AC）, covalent organic frameworks（COFs）, and metal-organic frameworks（MOFs）. We further demonstrated the application of our PTB-modified materials in the recovery of precious metals from low-grade gold ore and electronic waste leachates. Consequently, this strategy could increase the value of waste materials nearly 27 times. In addition, this method is generally extendable to other conventional industrial adsorbents such as resin, clay, and Al2O3, and enhances their adsorption capabilities at least twofold. Overall, this work provides a simple and green approach for improving the adsorption performance of solid particles, and is expected to develop into a universal strategy for transforming waste particles into high-value-added products.

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Molecular Cloning and Bioinformatics Analysis of cyaA Gene of Vibrio alginolyticus Strain HY9901

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Agricultural Biotechnology 2024年第4期13卷 6-10页

作者： Shi WANG Peng ZHOU yujia zhang Weijie zhang Na WANG Huanying PANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture&Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]This study was conducted to explore the biological functions of cyaA gene of Vibrio alginolyticus.[Methods]With DNA of *** HY 9901 as a template,primers were designed according to the sequence of cyaA gene,and the cyaA gene was amplified by *** analysis was performed.[Results]The cyaA gene of *** HY9901 was 2529 bp in size,and encoded 842 amino *** molecular structure of CyaA protein was C_(4358)H_(6745)N_(1171)O_(1286)S_(35).Its theoretical molecular weight was 97.24167 kDa and the theoretical pI value was *** had no signal peptide and transmembrane *** protein had three N-terminal glycosylation sites,one cAMP and cGMP-dependent protein kinase phosphorylation site,nine protein kinase C phosphorylation sites,nine casein kinase II phosphorylation sites,one tyrosine kinase phosphorylation site,seven N-terminal myristoylation sites,one pentenyl binding site and ten microbody C-terminal localization signal *** localization prediction showed that CyaA protein was mainly located in the nucleus and *** multi-sequence alignment and phylogenetic tree construction,it was concluded that *** had high CyaA homology with other Vibrio *** of *** was clustered with Vibrio fluminensis and Vibrio marinisedimini,and they were closely *** secondary structure of CyaA protein consisted ofα-helixes(43.11%),random coils(38.00%)and extended strands(14.49%).In protein network interaction,it was found that the proteins adjacent to CyaA protein were Crp-2,CpdA,Crr,PtsG-2,ANP67209.1,Crp-1,PykF,Pyk,RelA and Ndk.[Conclusions]This study provides a new idea for formulating strategies for the prevention and control of vibriosis.

关键词： Vibrio alginolyticus cyaA gene Bioinformatics analysis

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Theoretical analysis of frequency modulation-to-amplitude modulation on the final optics and target of the SG Ⅱ-Up laser facility

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High Power Laser Science and Engineering 2024年第1期12卷 84-98页

作者： yujia zhang Wei Fan Jiangfeng Wang Xiaochao Wang Xinghua Lu Dajie Huang Shouying Xu Yanli zhang Mingying Sun Zhaoyang Jiao Shenlei Zhou Xiuqing Jiang Key Laboratory of High Power Laser and Physics Shanghai Institute of Optics and Fine MechanicsChinese Academy of SciencesShanghaiChina Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijingChina

Frequency modulation(FM)-to-amplitude modulation(AM) conversion is an important factor that affects the time±power curve of inertial confinement fusion(ICF) high-power laser facilities. This conversion can impact uniform compression and increase the risk of damage to optics. However, the dispersive grating used in the smoothing by spectral dispersion technology will introduce a temporal delay and can spatially smooth the target. The combined effect of the dispersive grating and the focusing lens is equivalent to a Gaussian low-pass filter, which is equivalent to 8 GHz bandwidth and can reduce the intensity modulation on the target to below 5% with 0.3 nm @ 3 GHz + 20 GHz spectrum phase modulation. The results play an important role in the testing and evaluating of the FM-to-AM on the final optics and the target, which is beneficial for comprehensively evaluating the load capacity of the facility and isentropic compression experiment for ICF.

关键词： dispersion grating frequency modulation-to-amplitude modulation conversion high-power laser facility inertial confinement fusion phase modulation

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A comprehensive diagnostic system of ultra-thin liquid sheet targets

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High Power Laser Science and Engineering 2024年第3期12卷 41-50页

作者： Ziyang Peng Zhengxuan Cao Xuan Liu Yinren Shou Jiarui Zhao Shiyou Chen Ying Gao Pengjie Wang Zhusong Mei Zhuo Pan Defeng Kong Shirui Xu Zhipeng Liu Yulan Liang Tianqi Xu Tan Song Xun Chen Qingfan Wu yujia zhang Zihao zhang Xueqin Yan Wenjun Ma State Key Laboratory of Nuclear Physics and Technology School of PhysicsPeking UniversityBeijingChina National Key Laboratory of Shock Wave and Detonation Physics Institute of Fluid PhysicsChina Academy of Engineering PhysicsMianyangChina Center for Relativistic Laser Science Institute for Basic ScienceGwangjuRepublic of Korea Institute of Radiation Physics Helmholtz-Zentrum Dresden RossendorfDresdenGermany Beijing Laser Acceleration Innovation Center BeijingChina Institute of Guangdong Laser Plasma Technology GuangzhouChina

To meet the demands of laser-ion acceleration at a high repetition rate,we have developed a comprehensive diagnostic system for real-time and in situ monitoring of liquid sheet targets(LSTs).The spatially resolved rapid characterizations of an LST’s thickness,flatness,tilt angle and position are fulfilled by different subsystems with high *** the help of the diagnostic system,we reveal the dependence of thickness distribution on collision parameters and report the 238-nm liquid sheet generated by the collision of two liquid *** methods for the flatness and tilt angle of LSTs have also been provided,which are essential for applications of laser-driven ion acceleration and others.

关键词： diagnostics laser-ion acceleration liquid sheet targets

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《不安之书》汉译历程中的文本转换

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山东外语教学 2024年第4期45卷 123-133页

作者：张羽佳山东师范大学外国语学院山东济南250356 澳门理工大学语言及翻译学院中国澳门

葡萄牙现代主义作家费尔南多·佩索阿最具代表性的遗作《不安之书》问世于1982年,迄今已有七个不同的汉译本。由于历次汉译事件发生的语境、采用的底本等存在差异,汉译本中既有自葡文直译又有自英文转译,既有全译又有选译,既有同一译者... 详细信息

葡萄牙现代主义作家费尔南多·佩索阿最具代表性的遗作《不安之书》问世于1982年,迄今已有七个不同的汉译本。由于历次汉译事件发生的语境、采用的底本等存在差异,汉译本中既有自葡文直译又有自英文转译,既有全译又有选译,既有同一译者的重译又有不同译者的复译。本文首先回顾翻译史研究的一般原则,然后借助副文本调查和文本细读两种具体方法,尝试梳理《不安之书》的汉译历程并侧重其中文本的语言及组织转换情况,以期为汉语语境下的《不安之书》翻译乃至文学研究奠定基础。

关键词：佩索阿不安之书直译与转译重译与复译翻译史研究

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协同综合治理:中国海洋环境保护制度建构的新路径

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河南司法警官职业学院学报 2024年第1期22卷 116-122页

作者：张雨嘉北京化工大学北京100013

协同综合治理强调整合和平衡不同的海洋利用需求,实行跨部门统一规划、各利益相关者通力合作和统筹实施综合管理,是贯彻落实海洋强国战略的重要理论指导。2023年中国《海洋环境保护法》的修订,将陆海统筹、综合治理、公众参与纳入海洋... 详细信息

协同综合治理强调整合和平衡不同的海洋利用需求,实行跨部门统一规划、各利益相关者通力合作和统筹实施综合管理,是贯彻落实海洋强国战略的重要理论指导。2023年中国《海洋环境保护法》的修订,将陆海统筹、综合治理、公众参与纳入海洋环境保护的基本原则与具体制度安排中,要以此次修法为契机,强化协同、综合、统筹三大理念,加快推进中国海洋治理体系和治理能力现代化的进程。

关键词：协同综合治理海洋环境保护制度《海洋环境保护法》

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基于空化时空分布特征的调节阀空蚀风险预测

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华中科技大学学报（自然科学版） 2024年第6期52卷 71-79页

作者：张雨佳刘秀梅李贝贝刘申中国矿业大学机电工程学院江苏徐州221116

针对煤液化工程中的关键设备高压差调节阀耐空蚀能力差、使用寿命短的问题,利用高速相机拍摄获取调节阀空化流场图像,提出空隙率λ表征气相体积分数、空隙率变化速率β表征空蚀风险指标,实现了调节阀空蚀破坏的准确预测.结果表明:空隙... 详细信息

针对煤液化工程中的关键设备高压差调节阀耐空蚀能力差、使用寿命短的问题,利用高速相机拍摄获取调节阀空化流场图像,提出空隙率λ表征气相体积分数、空隙率变化速率β表征空蚀风险指标,实现了调节阀空蚀破坏的准确预测.结果表明:空隙率数值与气相体积分数正相关;随着入口压力的增大,阀芯头部和喉部对应空隙率数值增大,空化得到充分发展向扩张段延伸并溃灭,调节阀内空化呈初生、发展、脱落和溃灭的周期性变化趋势.当空隙率变化速率β<0时,表示空泡处于溃灭状态,据此可以判断该处存在空蚀风险;随着入口压力的增加,附着型空泡溃灭位置较为固定在调节阀头部,是阀芯发生空蚀破坏的主要原因.游离型空化损伤区域随着入口压力增加逐渐向调节阀下游延伸,调节阀流道上方和下方空化损伤区域为非对称结构,下方的空化损伤区域整体超前于上方.

关键词：空化调节阀空隙率空蚀破坏风险预测

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非遗蜀绣的传承发展路径研究

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西部皮革 2024年第14期46卷 30-32页

作者：张雨嘉武汉轻工大学人文与传媒学院

蜀绣作为四川地区重要的非物质文化遗产，在可持续发展的道路上遇到了诸多挑战。通过实地考察和问卷调查，文章探讨了蜀绣在当代传承发展的困境。通过调查发现，蜀绣面临着传承中断、人才短缺以及民族文化意识薄弱等问题。为了应对这些... 详细信息

蜀绣作为四川地区重要的非物质文化遗产，在可持续发展的道路上遇到了诸多挑战。通过实地考察和问卷调查，文章探讨了蜀绣在当代传承发展的困境。通过调查发现，蜀绣面临着传承中断、人才短缺以及民族文化意识薄弱等问题。为了应对这些挑战，我们提出了构建完善的保护机制、整合创新技艺、推动宣传方式的变革以及加强民族文化教育等建议，旨在让蜀绣在新时代焕发出新的活力。

关键词：蜀绣非物质文化遗产民族文化

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低温试验室内小型风洞设计及结冰过程试验

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武汉理工大学学报（交通科学与工程版） 2024年第2期48卷 292-297页

作者：陈啸东钱作勤张羽佳武汉理工大学船海与能源动力工程学院武汉430063

在低温试验室中利用自搭建风洞设备提供水结冰所需的风速和温度条件,研究了极区风速、温度对水结冰速率的影响.结果表明:结冰池经历了整体快速降温和结冰两个阶段.在前一阶段对流换热占主体换热地位,风速影响大于温度影响;后一阶段随冰... 详细信息

在低温试验室中利用自搭建风洞设备提供水结冰所需的风速和温度条件,研究了极区风速、温度对水结冰速率的影响.结果表明:结冰池经历了整体快速降温和结冰两个阶段.在前一阶段对流换热占主体换热地位,风速影响大于温度影响;后一阶段随冰厚增加热传导占比逐渐上升,温度的影响也逐渐上升.结合温度变化与冰厚生长数据,计算得出降温速度与结冰速度,结合温度与风速分别进行曲面拟合,得到降温速度、结冰速度关于温度、风速的关系式.

关键词：结冰过程风洞试验风速温度

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