检索结果-南通市图书馆

Npac Is A Co-factor of Histone H3K36me3 and Regulates Transcriptional Elongation in Mouse Embryonic Stem Cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2022年第1期20卷 110-128页

作者： Sue Yu Jia Li Guanxu Ji Zhen Long Ng Jiamin Siew Wan Ning Lo Ying Ye Yuan Yuan Chew Yun Chau Long Wensheng Zhang Ernesto Guccione yuin han loh Zhi-Hong Jiang Henry Yang Qiang Wu Department of Biochemistry Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117597Singapore Cancer Science Institute of Singapore Centre for Translational MedicineSingapore 117599Singapore The State Key Laboratory of Quality Research in Chinese Medicine Macao University of Science and TechnologyMacao Special Administrative Region 999078China Cam-Su Genomic Resource Center Soochow UniversitySuzhou 215123China Institute of Molecular and Cell Biology Singapore 138673Singapore

Chromatin modification contributes to pluripotency maintenance in embryonic stem cells(ESCs).However,the related mechanisms remain ***,we show that Npac,a"reader"of histone H3 lysine 36 trimethylation(H3K36me3),is required to maintain mouse ESC(mESC)pluripotency since knockdown of Npac causes mESC *** of Npac in mouse embryonic fibroblasts(MEFs)inhibits reprogramming ***,our chromatin immunoprecipitation followed by sequencing(ChIP-seq)results of Npac reveal that Npac co-localizes with histone H3K36me3 in gene bodies of actively transcribed genes in ***,we find that Npac interacts with positive transcription elongation factor b(p-TEFb),Ser2-phosphorylated RNA PolⅡ(RNA PolⅡSer2P),and Ser5-phosphorylated RNA PolⅡ(RNA PolⅡSer5 P).Furthermore,depletion of Npac disrupts transcriptional elongation of the pluripotency genes Nanog and *** together,we propose that Npac is essential for the transcriptional elongation of pluripotency genes by recruiting p-TEFb and interacting with RNA PolⅡSer2P and Ser5P.

关键词： Npac Pluripotency Reprogramming Histone H3K36me3 Transcriptional elongation

Overcoming barriers to the clinical utilization of iPSCs:reprogramming efficiency,safety and quality

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2012年第11期3卷 834-845页

作者： Suying Cao Kyle loh Yangli Pei Wei Zhang Jianyong han State Key Laboratory for Agrobiotechnology College of Biological SciencesChina Agricultural UniversityBeijing 100193China Stem Cell and Developmental Biology Genome Institute of Singapore138672Singapore

Differentiated cells can be reprogrammed into pluripotent stem cells,known as“induced pluripotent stem cells”(iPSCs),through the overexpression of defined transcription *** creation of iPSC lines has opened new avenues for patient-specific cell replacement therapies for regenerative ***,the clinical utilization of iPSCs is largely impeded by two *** first limitation is the low efficiency of iPSCs generation from differentiated *** second limitation is that many iPSC lines are not authentically pluripotent,as many cell lines inefficiently differentiate into differentiated cell types when they are tested for their ability to complement embryonic ***,the“quality”of iPSCs must be increased if they are to be differentiated into specialized cell types for cell replacement *** these two limitations is paramount to facilitate the widespread employment of iPSCs for therapeutic ***,we summarize recent progress made in strategies enabling the efficient production of high-quality iPSCs,including choice of reprogramming factors,choice of target cell type,and strategies to improve iPSC quality.

关键词： iPSCs reprogramming embryonic stem cells efficiency and quality

Robust n-type doping of WSe2 enabled by controllable proton irradiation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nano Research 2023年第1期16卷 1220-1227页

作者： Haidong Liang Yue Zheng Leyi loh Zehua Hu Qijie Liang Cheng han Michel Bosman Wei Chen Andrew A.Bettiol Centre for Ion Beam Applications(CIBA) Department of PhysicsNational University of SingaporeSingapore 117542Singapore International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Institute of Microscale OptoelectronicsShenzhen UniversityShenzhen 518060China Department of Physics National University of SingaporeSingapore 117551Singapore Department of Materials Science and Engineering National University of SingaporeSingapore 117575Singapore Department of Chemistry National University of SingaporeSingapore 117543Singapore Division of Physics and Applied Physics School of Physical and Mathematical ScienceNanyang Technological UniversitySingapore 637371Singapore Songshan Lake Materials Laboratory Songshan Lake Mat LabDongguan 523808China National University of Singapore(Suzhou)Research Institute 377 Lin Quan StreetSuzhou Industrial ParkSuzhou 215123China

Two-dimensional(2D)transition metal dichalcogenides(TMDs)are considered to be promising building blocks for the next generation electronic and optoelectronic *** doping schemes and work function engineering techniques have been explored to overcome the intrinsic performance limits of 2D ***,a reliable and long-time air stable doping scheme is still lacking in this *** this work,we utilize keV ion beams of H2+to irradiate layered WSe2 crystals and obtain efficient n-type doping effect for all irradiated crystals within a fluence of 1×1014 protons·cm−2(1e14).Moreover,the irradiated WSe2 remains an n-type semiconductor even after it is exposed to ambient conditions for a *** ion irradiation with a focused beam can directly pattern on the sample to make high performance homogenous p-n junction *** and photoluminescence(PL)spectra demonstrate that the WSe2 crystal lattice stays intact after irradiation within *** attribute the reliable electrondoping to the significant increase in Se vacancies after the proton irradiation,which is confirmed by our scanning transmission electron microscope(STEM)*** work demonstrates a reliable and long-term air stable n-type doping scheme to realize high-performance electronic TMD devices,which is also suitable for further integration with other 2D devices.

关键词： WSe2 proton beam irradiation n-type doping long-time air stable Se vacancies

Monolayer Graphene as a Saturable Absorber in a Mode-Locked Laser

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2011年第3期4卷 297-307页

作者： Qiaoliang Bao han Zhang Zhenhua Ni Yu Wang Lakshminarayana Polavarapu Zexiang Shen Qing-Hua Xu Dingyuan Tang Kian Ping loh Department of Chemistry National University of Singapore 3 Science Drive 3 Singapore 117543 Singapore School of Electrical and Electronic Engineering Nanyang Technological University Singapore 639798 Singapore School of Physical and Mathematical Sciences Nanyang Technological University Singapore 637371 Singapore These authors contributed equally to this work.

We demonstrate that the intrinsic properties of monolayer graphene allow it to act as a more effective saturable absorber for mode-locking fiber lasers when compared to multilayer graphene. The absorption of monolayer graphene can be saturated at lower excitation intensity compared to multilayer graphene, graphene with wrinkle-like defects, or functionalized graphene. Monolayer graphene has a remarkably large modulation depth of 65.9%, whereas the modulation depth of multilayer graphene is greatly reduced due to nonsaturable absorption and scattering loss. Picosecond ultrafast laser pulses （1.23 ps） can be generated using monolayer graphene as a saturable absorber. Due to the ultrafast relaxation time, larger modulation depth and lower scattering loss of monolayer graphene, it performs better than multilayer graphene in terms of pulse shaping ability, pulse stability, and output energy.

关键词： Graphene saturable absorber laser carrier dynamics ultrafast photonics

Controlling phase transition in WSe_(2) towards ideal n-type transistor

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2021年第8期14卷 2703-2710页

作者： Yue Zheng Du Xiang Jialin Zhang Rui Guo Wenhui Wang Tao Liu Leyi loh Yanan Wang Jing Gao Cheng han Michel Bosman Zhenhua Ni Wei Chen SZU-NUS Collaborative Innovation Center for Optoelectronic Science&Technology International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of EducationInstitute of Microscale OptoelectronicsShenzhen UniversityShenzhen 518060China Department of Physics National University of SingaporeSingapore 117551Singapore Department of Chemistry National University of SingaporeSingapore 117543Singapore School of Physics and the Key Laboratory of MEMS of the Ministry of Education Southeast UniversityNanjing 210096China Department of Materials Science and Engineering National University of Singapore Singapore 117575Singapore Joint School of National University of Singapore and Tianjin University International Campus of Tianjin UniversityBinhai New CityFuzhou 350207China National University of Singapore(Suzhou)Research Institute 377Lin Quan StreetSuzhou Industrial ParkSuzhou 215123China

Two-dimensional(2D)transition metal dichalcogenides(TMDs)have been rapidly established as promising building blocks for versatile atomic scale circuits and multifunctional ***,the high contact resistance in TMDs based transistors seriously hinders their applications in complementary *** this work,we show that an Ohmic homojunction n-type tungsten diselenide(WSe_(2))transistor is realized through spatially controlling cesium(Cs)doping region near the *** find that the remarkable electron doping effect of Cs stimulates a semiconductor to metal(2H to 1T')phase transition in WSe_(2),and hence the formation of 2H-1T’hetero-phase *** method significantly optimizes the WSe_(2) transport behavior with a perfect low subthreshold swing of-61 mV/dec and ultrahigh current on/off ratio exceeding-10^(9).Meanwhile,the electron mobility is enhanced by nearly 50 *** elucidate that the ideal n-type behavior originates from the negligible Schottky barrier height of~19 meV and low contact resistance of-0.9Ωk·μm in the 2H-1T’homojunction ***,based on the Ohmic hetero-phase configuration,a WSe_(2) inverter is achieved with a high gain of~270 and low power consumption of-28 *** findings envision Cs functionalization as an effective method to realize ideal Ohmic contact in 2D WSe_(2) transistors towards high performance complementary electronic devices.

关键词： WSe_(2) Cs intercalation semiconductor to metal phase transition ideal hetero-phase transistor high gain homo-inverter

Diffraction-limited imaging with monolayer 2D material-based ultrathin flat lenses

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Light(Science & Applications) 2020年第1期9卷 696-706页

作者： han Lin Zai-Quan Xu Guiyuan Cao Yupeng Zhang Jiadong Zhou Ziyu Wang Zhichen Wan Zheng Liu Kian Ping loh Cheng-Wei Qiu Qiaoliang Bao Baohua Jia Centre for Translational Atomaterials Faculty of ScienceEngineering and TechnologySwinburne University of TechnologyP.O.Box 218HawthornVIC 3122Australia Department of Materials Science and Engineering ARC Centre of Excellence in Future Low-Energy Electronics Technologies(FLEET)Monash UniversityWellington RoadClaytonVIC 3800Australia School of Mathematical and Physical Sciences Faculty of ScienceUniversity of Technology Sydney15 BroadwayUltimoNSW 2007Australia Institute of Microscale Optoelectronics Lab of Artificial Microstructure for OptoelectronicsShenzhen University518000 ShenzhenChina School of Materials Science and Engineering Nanyang Technological UniversitySingapore 639798Singapore Department of Chemistry National University of SingaporeSingapore 117543Singapore Department of Electrical and Computer Engineering National University of SingaporeSingapore 117583Singapore The Australian Research Council(ARC)Industrial Transformation Training Centre in Surface Engineering for Advanced Materials(SEAM) Swinburne University of TechnologyP.O.Box 218HawthornVIC 3122Australia

Ultrathin flat optics allow control of light at the subwavelength scale that is unmatched by traditional refractive *** approach the atomically thin limit,the use of 2D materials is an attractive possibility due to their high refractive ***,achievement of diffraction-limited focusing and imaging is challenged by their thickness-limited spatial resolution and focusing *** we report a universal method to transform 2D monolayers into ultrathin flat *** laser direct writing was applied to generate local scattering media inside a monolayer,which overcomes the longstanding challenge of obtaining sufficient phase or amplitude modulation in atomically thin 2D *** achieved highly efficient 3D focusing with subwavelength resolution and diffractionlimited *** high focusing performance even allows diffraction-limited imaging at different focal positions with varying *** work paves the way for downscaling of optical devices using 2D materials and reports an unprecedented approach for fabricating ultrathin imaging devices.

关键词： ultrathin scattering optics

A　Combination　Method　of　SVD　And　Neuro－Controller　for　Identifying　Uncertain　Systems

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Systems Engineering and Electronics 1995年第2期6卷 40-51页

作者： Lin Wang, Min han, Robert N.K. loh (Center for Robottes and Advanced Automation, Oakland University, Michigan 48309, U.S.A.) Center for Robotics and Advanced Automation Oakland University Michigan 48309 U.S.A.

A combination method of Singular Value Decomposition (SVD) and Neuro-Controller is presented in this *** a system with unknown structure and uncertain parameters,a SVD and backward linear prediction (BLP) method (SVD-BLP method) is used to determine the order of the system in a noisy environment. Then a neuro-controller is applied to dynamically idelltify is the uncertain parameters of the system in the face of disturbances. Simulation results are presented.

关键词： SVD Neural network Adaptive control Backward linear prediction (BLP).

Effectiveness of an ocular adhesive polyhedral oligomeric silsesquioxane hybrid thermo-responsive FK506 hydrogel in a murine model of dry eye

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bioactive Materials 2022年第3期7卷 77-91页

作者： Yi han Lu Jiang Huihui Shi Chenfang Xu Minting Liu Qingjian Li Lan Zheng Hong Chi Mingyue Wang Zuguo Liu Mingliang You Xian Jun loh Yun-Long Wu Zibiao Li Cheng Li Fujian Provincial Key Laboratory of Ophthalmology and Visual Science&Ocular Surface and Corneal Diseases Eye Institute&Affiliated Xiamen Eye CenterSchool of MedicineXiamen UniversityXiamen361102China Institute of Materials Research and Engineering A*STAR(Agency for ScienceTechnology and Research)Singapore138634Singapore School of Chemical Sciences University of Chinese Academy of ScienceBeijing100049China Key Laboratory of Marine Materials and Related Technologies Zhejiang Key Laboratory of Marine Materials and Protective TechnologiesNingbo Institute of Material Technology and EngineeringChinese Academy of ScienceNingbo315201China Fujian Provincial Key Laboratory of Innovative Drug Target Research and State Key Laboratory of Cellular Stress Biology School of Pharmaceutical SciencesXiamen UniversityXiamen361102China hangzhou Cancer Institute Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang ProvinceAffiliated Hangzhou Cancer HospitalZhejiang University School of MedicineHangzhou310002China Shandong Provincial Key Laboratory of Molecular Engineering School of Chemistry and Pharmaceutical EngineeringQilu University of Technology(Shandong Academy of Sciences)Jinan250353China Department of Materials Science and Engineering National University of SingaporeSingapore117575Singapore

Dry eye is a common ocular disease that results in discomfort and impaired vision,impacting an individual’s quality of life.A great number of drugs administered in eye drops to treat dry eye are poorly soluble in water and are rapidly eliminated from the ocular surface,which limits their therapeutic ***,it is imperative to design a novel drug delivery system that not only improves the water solubility of the drug but also prolongs its retention time on the ocular ***,we develop a copolymer from mono-functional POSS,PEG,and PPG(MPOSS-PEG-PPG,MPEP)that exhibits temperature-sensitive sol-gel transition *** thermo-responsive hydrogel improves the water solubility of FK506 and simultaneously provides a mucoadhesive,long-acting ocular delivery *** addition,the FK506-loaded POSS hydrogel possesses good biocompatibility and significantly improves adhesion to the ocular *** comparison with other FK506 formulations and the PEG-PPG-FK506(F127-FK506)hydrogel,this novel MPOSS-PEG-PPG-FK506(MPEP-FK506)hydrogel is a more effective treatment of dry eye in the murine dry eye ***,delivery of FK506 in this POSS hydrogel has the potential to prolong drug retention time on the ocular surface,which will improve its therapeutic efficacy in the management of dry eye.

关键词： Ocular adhesive Polyhedral oligomeric silsesquioxane FK506 Dry eye

Rationales for expression and altered expression of apoptotic protease activating factor-1 gene in gastric cancer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2007年第38期13卷 5060-5064页

作者： He-Ling Wang han Bai Yan Li Jun Sun Xue-Qing wang Department of Gastroenterology Shengjing Hospital Affiliated to China Medical University Shenyang 110004 Liaoning Province China Department of Infectious Diseases Shengjing Hospital Affiliated to China Medical University Shengyang 110004 Liaoning Province China

AIM: To elucidate the relationship between apoptotic protease activating factor-1 (Apaf-1) gene and gastric cancer. METHODS: Thirty-five postoperative cancer and adjacent normal tissue samples were collected in the present study. Expression of the Apaf-1 gene in these samples was analyzed by semi-quantitative RT-PCR. Loss of heterozygosity (loh) was used to determine whether there was loss of Apaf-1 gene in domain of 12q22-23 in the samples. Promoter methylation of Apaf-1 gene in the samples was analyzed by methylation specific (MSP) PCR. RESULTS: The expression of Apaf-1 mRNA in gastric cancer tissue samples was 51%. The loh frequency of D12S346, D12S1706, D12S327, D12S1657 and D12S393 was 33%, 8%, 58%, 12% and 42%, respectively. Fifty percent loh was found at two sites and 17% loh at three sites. Apaf-1 mRNA expression decreased significantly in 13 cases (rs = 0.487, P = 0.003). The rate of Apaf-1 promoter methylation was 49% in gastric cancer tissue samples and 23% in para-cancerous tissue samples. Promoter methylation occurred significantly in 16 of 18 gastric cancer tissue samples with decreased expression of Apaf-1 mRNA rs = 0.886, P = 10-6). CONCLUSION: The expression of Apaf-1 gene is low in gastric cancer tissues. Methylation of Apaf-1 gene promoter and loh in domain of 12q22-23 are the main reasons for the expression and altered expression of Apaf-1 gene.

关键词： Gastric cancer Apaf-1 gene Loss of heterozygosity Methylation