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ALK gene expression status in pleural effusion predicts tumor responsiveness to crizotinib in Chinese patients with lung adenocarcinoma

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Chinese Journal of Cancer Research 2016年第6期28卷 606-616页

作者： Zheng Wang Xiaonan Wu Xiaohong Han Gang Cheng Xinlin Mu yuhui Zhang Di cui Chang Liu Dongge Liu Yuankai Shi Department of Pathology Beijing HospitalNational Center of Gerontology Department of Medical Oncology Beijing HospitalNational Center of Gerontology Department of Medical Oncology National Cancer Center/Cancer Institute &Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Department of Respiratory and Critical Care Medicine Peking University People's Hospital Beijing Institute of Respiratory Medicine Beijing Chao-Yang Hospital Capital Medical University Department of Medical Oncology PLA Army General Hospital

Objective： The relationship between anaplastic lymphoma kinase（ALK） expression in malignant pleural effusion（MPE） samples detected only by Ventana immunohistochemistry（IHC） ALK（D5F3） and the efficacy of ALKtyrosine kinase inhibitor therapy is ***： Ventana anti-ALK（D5F3） rabbit monoclonal primary antibody testing was performed on 313 cell blocks of MPE samples from Chinese patients with advanced lung adenocarcinoma, and fluorescence in situ hybridization（FISH） was used to verify the ALK gene status in Ventana IHC ALK（D5F3）-positive samples. The follow-up clinical data on patients who received crizotinib treatment were ***： Of the 313 MPE samples, 27（8.6%） were confirmed as ALK expression-positive, and the Ventana IHC ALK（D5F3）-positive rate was 17.3%（27/156） in wild-type epidermal growth factor receptor（EGFR） MPE samples. Twenty-three of the 27 IHC ALK（D5F3）-positive samples were positive by FISH. Of the 11 Ventana IHC ALK（D5F3）-positive patients who received crizotinib therapy, 2 patients had complete response（CR）, 5 had partial response（PR） and 3 had stable disease（SD）.Conclusions： The ALK gene expression status detected by the Ventana IHC ALK（D5F3） platform in MPE samples may predict tumor responsiveness to crizotinib in Chinese patients with advanced lung adenocarcinoma.

关键词： Anaplastic lymphoma kinase fluorescence in situ hybridization immunohistochemistry lung adenocarcinoma pleural effusion crizotinib

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Transcriptome-wide evolutionary analysis on essential brown algae(Phaeophyceae)in China

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Acta Oceanologica Sinica 2014年第2期33卷 13-19页

作者： SUN Jing WANG Liang WU Shuangxiu WANG Xumin XIAO Jingfa CHI Shan LIU cui REN Lufeng ZHAO yuhui LIU Tao YU Jun CAS Key Laboratory of Genome Sciences and Information Beijing Key Laboratory of Genome and Precision Medicine TechnologiesBeijing Institute of GenomicsChinese Academy of Sciences Beijing Key Laboratory of Functional Genomics for Dao-di Herbs Beijing Institute of GenomicsChinese Academy of Sciences University of Chinese Academy of Sciences College of Marine Life Science Ocean University of China

Brown algae （Chromista, Ochrophyta, Phaeophyceae） are a large group of multicellular algae that play im-portant roles in the ocean＇s ecosystem and biodiversity. However, poor molecular bases for studying their phylogenetic evolutions and novel metabolic characteristics have hampered progress in the field. In this study, we sequenced the de novo transcriptome of 18 major species of brown algae in China, covering six orders and seven families, using the high-throughput sequencing platform Illumina HiSeq 2000. From the transcriptome data of these 18 species and publicly available genome data of Ectocarpus siliculosus and Phaeodactylum tricornutum, we identified 108 nuclear-generated orthologous genes and clarified the phy-logenetic relationships among these brown algae based on a multigene method. These brown algae could be separated into two clades：Clade Ishigeales-Dictyotales and Clade Ectocarpales-Laminariales-Desmares-tiale-Fucales. The former was at the base of the phylogenetic tree, indicating its early divergence, while the latter was divided into two branches, with Order Fucales diverging from Orders Ectocarpales, Laminariales, and Desmarestiale. In our analysis of taxonomy-contentious species, Sargassum fusiforme and Saccharina sculpera were found to be closely related to genera Sargassum and Saccharina, respectively, while Petalonia fascia showed possible relation to genus Scytosiphon. The study provided molecular evidence for the phylo-genetic taxonomy of brown algae.

关键词： Phaeophyceae transcriptome sequencing multigene phylogeny

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新型手术机器人系统自主置入胸椎椎弓根螺钉的准确性研究

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生物骨科材料与临床研究 2022年第2期19卷 5-10页

作者：李宗泽陈建庭朱青安朱永健崔玉辉侯崛东孙东辉陈嘉瑞南方医科大学南方医院骨科-脊柱骨科广东广州510515 南方医科大学南方医院影像诊断科广东广州510515 深圳市鑫君特智能医疗器械有限公司广东深圳518000

目的评估新型手术机器人机器人系统用于置入胸椎椎弓根螺钉的准确性。方法选取3具成人脊柱标本行CT扫描并三维重建。在术者监视下,机器人遵循术前规划,于各胸椎椎弓根经皮自主置入克氏针,完成开路扩孔、攻丝和经皮置钉。置钉后再行薄层C... 详细信息

目的评估新型手术机器人机器人系统用于置入胸椎椎弓根螺钉的准确性。方法选取3具成人脊柱标本行CT扫描并三维重建。在术者监视下,机器人遵循术前规划,于各胸椎椎弓根经皮自主置入克氏针,完成开路扩孔、攻丝和经皮置钉。置钉后再行薄层CT扫描,测量螺钉破壁情况评估置钉准确性,螺钉位置准确性的评价基于Gertzbein-Robbins分级标准。比较上胸椎(T_(1)-T_(4))、中胸椎(T_(5)-T_(8))、下胸椎(T_(9)-T_(12))螺钉置入准确性的差异。结果共置入胸椎椎弓根螺钉72枚,螺钉位置临床可接受率(A级或B级)为84.7%。胸椎不同分段间螺钉位置临床可接受率的差异无统计学意义(P>0.05),但下胸椎置钉优秀率(A级)更高(P<0.05)。结论携带自主动力装置的新型手术机器人系统可准确置入胸椎椎弓根螺钉,置钉准确率与胸椎分段无关。

关键词：手术机器人椎弓根螺钉胸椎准确性

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Response Variability in Dorsal Parietal Area5 during Reaching Arm Movement

Response Variability in Dorsal Parietal Area5 during Reachin...

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中国神经科学学会第十二届全国学术会议

作者： Chenyang Li yuhui Li He cui CAS Key Laboratory of Primate Neurobiology Institute of Neuroscience Chinese Academy of Sciences

Neural responses are always classified by their mean firing rate. Many research estimate the relationship between behavior and neural firing rate, but rarely check response variability. Using Fano factor as the criterion, it has been found that stimulus onset reduces firing-rate variability in 13 extracellularly recorded datasets, indicating it can be a common feature of cortical response. To examine whether variability response in dorsal parietal area5 decline during stimulus onset, we analyzed single-unit data recorded from dorsal parietal area5 while a monkey performed double-and single-reach task as the dataset（Li and cui, J Neurosci 2013） and computed its Fano factor in a sliding time window. Spike timestamp was transferred to a time series with 1 ms time bin width. Then we choose-500 to 500 ms around a chosen event timestamp from the time series to establish a structure containing a field ‘spikes’, which is a matrix with all trials in one condition. These structures corresponding to every tasks and conditions（4 x8, four trial types by eight directions） would be collected to build a structure array as the input data. To compute Fano factor throughout the trial, we chose a 30-ms window sliding with a 25-ms step. In the present study, our dataset derived from the recording data in dorsal parietal area5 contained two kinds of paradigm. The difference between two paradigms was whether monkey needed to keep fixation at the screen center while it is completing center-out reach task and double reach task（first reach was like center out but the second target appeared at 120° clockwise of first target）. In other words, after data analysis, our dataset divided to four input datasets, according to different trial type. Every event was chosen to build a structure array for Fano factor computing. However, all events, believed as the stimulus onset, didn’t drive a decline in firing-rate variability, estimated by Fano factor. These results suggested that firing-rate variabili

关键词： Fano factor Monkey Single-unit recording Neurophysiology

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The Association Between H3K4me3 and Antisense Transcription

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Genomics, Proteomics & Bioinformatics 2012年第2期10卷 74-81页

作者： Peng cui Wanfei Liu yuhui Zhao Qiang Lin Feng Ding Chengqi Xin Jianing Geng Shuhui Song Fanglin Sun Songnian Hu Jun Yu CAS Key Laboratory of Genome Sciences and Information Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100029China Institute of Epigenetics and Cancer Research School of MedicineTsinghua UniversityBeijing 100080China Graduate University of Chinese Academy of Sciences Beijing 100049China

Histone H3 lysine 4 trimethylation （H3K4me3） is well known to occur in the promoter region of genes for transcription activation. How- ever, when investigating the H3K4me3 profiles in the mouse cerebrum and testis, we discovered that H3K4me3 also has a significant enrichment at the 3＇ end of actively transcribed （sense） genes, named as 3＇-H3K4me3. 3＇-H3K4me3 is associated with ~15% of pro- tein-coding genes in both tissues. In addition, we examined the transcriptional initiation signals including RNA polymerase II （RNAPII） binding sites and Y-CAGE-tag that marks transcriptional start sites. Interestingly, we found that 3＇-H3K4me3 is associated with the ini- tiation of antisense transcription. Furthermore, 3＇-H3K4me3 modification levels correlate positively with the antisense expression levels of the associated sense genes, implying that 3＇-H3K4me3 is involved in the activation of antisense transcription. Taken together, our findings suggest that H3K4me3 may be involved in the regulation of antisense transcription that initiates from the 3＇ end of sense genes. In addition, a positive correlation was also observed between the expression of antisense and the associated sense genes with 3＇-H3K4me3 modification. More importantly, we observed the 3＇-H3K4me3 enrichment among genes in human, fruitfly and Arabidopsis, and found that the sequences of 3＇-H3K4me3-marked regions are highly conserved and essentially indistinguishable from known promoters in ver- tebrate. Therefore, we speculate that these 3＇-H3K4me3-marked regions may serve as potential promoters for antisense transcription and 3＇-H3K4me3 appear to be a universal epigenetic feature in eukaryotes. Our results provide a novel insight into the epigenetic roles of H3K4me3 and the regulatory mechanism of antisense transcription.

关键词： Antisense initiation and activation Antisense transcription H3K4me3

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Comparative Analyses of H3K4 and H3K27 Trimethylations Between the Mouse Cerebrum and Testis

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Genomics, Proteomics & Bioinformatics 2012年第2期10卷 82-93页

作者： Peng cui Wanfei Liu yuhui Zhao Qiang Lin Daoyong Zhang Feng Ding Chengqi Xin Zhang Zhang Shuhui Song Fanglin Sun Jun Yu Songnian Hu CAS Key Laboratory of Genome Sciences and Information Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100029China Institute of Epigenetics and Cancer Research School of MedicineTsinghua UniversityBeijing 100080China Computational Bioscience Research Center 4700 King Abdullah University of Science and TechnologyThuwa123955-6900Kingdom of Saudi Arabia Graduate University of Chinese Academy of Sciences Beijing 100049China

The global features of H3K4 and H3K27 trimethylations （H3K4me3 and H3K27me3） have been well studied in recent years, but most of these studies were performed in mammalian cell lines. In this work, we generated the genorne-wide maps of H3K4me3 and H3K27me3 of mouse cerebrum and testis using ChlP-seq and their high-coverage transcriptomes using ribominus RNA-seq with SOLID technology. We examined the global patterns of H3K4me3 and H3K27me3 in both tissues and found that modifications are closely-associated with tissue-specific expression, function and development. Moreover, we revealed that H3K4me3 and H3K27me3 rarely occur in silent genes, which contradicts the findings in previous studies. Finally, we observed that bivalent domains, with both H3K4me3 and H3K27me3, existed ubiquitously in both tissues and demonstrated an invariable preference for the regulation of developmentally-related genes. How- ever, the bivalent domains tend towards a ＂winner-takes-all＂ approach to regulate the expression of associated genes. We also verified the above results in mouse ES cells. As expected, the results in ES cells are consistent with those in cerebrum and testis. In conclusion, we present two very important findings. One is that H3K4me3 and H3K27me3 rarely occur in silent genes. The other is that bivalent domains may adopt a ＂winner-takes-all＂ principle to regulate gene expression.

关键词： H3K4me3 H3K27me3 Mouse

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A fast and highly efficient hiPSCs-Adipocytes differentiation method:patient specific cell models for adipogenesis and obesity research

A fast and highly efficient hiPSCs-Adipocytes differentiatio...

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第十三届江浙沪儿科学术会议暨2016年浙江省医学会儿科学学术年会

作者： Chenbo Ji yuhui Ni Yaping Zhao Xianwei cui Lianghui You Xing Wang Jiwu Wang Xirong Guo Nanjing Maternity and Child Health Care Hospital

Obesity epidemic is a growing global health *** far there is no safe and effective way to prevent obesity,as weight loss does not simply depend on diet or physical *** is characterized by an excessive accumulation of adipose tissue,therefore understanding the biological features of adipocytes is one of the keys to find the cure for *** in vitro models,such as 3 T3-L1,primary preadipocytes or some adult multipotent stem cells,contribute to our understanding of adipogenesis and the mechanisms of ***,they are either murine origin cell lines,or primary cells withlimited expansion,which caused many contradictory *** present study,we developed an improved method to get human induced pluripotent stem cells(hiPSCs)drived mesenchymal stem cells(MSCs) with high capacity of adipocyte differention(80-95%efficiency) after one day adipocyte differentiation *** gene expression signatures of the adipocytes differentiated from the hiPSCs-MSCs is highly similar to primary mature adipocytes,with sensitive insulin response and function of adipokines *** previous study demonstrated that C10 orf116 and miR-148 a facilitated adipogenesis in primary preadipocyte or *** use these two factors to test the application of our newly developed hiPSCsMSCs-Adipocyte model in adipogenesis *** addition,by comparing iPSCs lines from donors with different body mass index(BMI),we found the efficiency of adipogenic differention were highly related to BMI of the *** conclusion,this hiPSCs-MSCs-Adipocyte model we developed is fast,highly efficient,and with patient specific genetic background,which should be a great tool for adipogenesis research and other obesity mechanism or clinical research.

关键词： hiPSCs adipogenesis Obesity

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高龄冠心病合并房颤患者不同抗栓治疗方案的疗效及安全性研究

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重庆医学 2017年第5期46卷 607-608,611页

作者：罗羽慧崔坤梅霞重庆市人民医院心内科 400013

目的比较高龄冠心病合并房颤患者不同抗栓方案的疗效及安全性。方法选择该院完成2年随访的住院和门诊房颤合并冠心病患者共224例,根据服用抗栓药物将患者分为4组,即华法林组、华法林+单联组、单联组及对照组,观察4组患者心血管血栓事件... 详细信息

目的比较高龄冠心病合并房颤患者不同抗栓方案的疗效及安全性。方法选择该院完成2年随访的住院和门诊房颤合并冠心病患者共224例,根据服用抗栓药物将患者分为4组,即华法林组、华法林+单联组、单联组及对照组,观察4组患者心血管血栓事件及出血事件。结果对照组总血栓事件特别是新发脑梗死风险(12.50%)明显高于其他各组,差异有统计学意义(P<0.05)。华法林+单联组患者的血栓事件发生率(2.78%)低于华法林组(4.29%)和单联组(5.81%),但差异无统计学意义。对照组患者的总体出血风险(3.13%)明显低于其他抗栓组,差异具有统计学意义(P<0.05)。单联组患者皮肤瘀斑的发生率低于其他抗栓组,而华法林+单联组患者总体出血风险特别是消化道出血的发生率明显高于其他各组,差异均具有统计学意义(P<0.05)。结论冠心病合并房颤的高龄患者需要进行抗栓治疗以预防血栓事件的发生,使用华法林特别是华法林联合抗血小板药物抗栓治疗并未引发严重出血事件发生。

关键词：老年人心房颤动冠心病抗栓疗效安全性

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通过二维沸石调节铂的氢化选择性

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Science China Materials 2024年第7期67卷 2135-2141页

作者：翟玉慧马悦崔凤超田宇阳朱广山 Key Laboratory of Polyoxometalate and Reticular Material Chemistry of Ministry of Education Faculty of ChemistryNortheast Normal UniversityChangchun 130024China

不饱和醇是一类重要的医药中间体,然而C=C加氢比C=O加氢反应更具有热力学优势,因此由不饱和醛合成不饱和醇具有挑战性.为解决这一问题,需要寻求一种具有可调控选择性的氢化催化剂.二维沸石的高长径比和大量的末端基团作为锚定位点,使其... 详细信息

不饱和醇是一类重要的医药中间体,然而C=C加氢比C=O加氢反应更具有热力学优势,因此由不饱和醛合成不饱和醇具有挑战性.为解决这一问题,需要寻求一种具有可调控选择性的氢化催化剂.二维沸石的高长径比和大量的末端基团作为锚定位点,使其不仅能作为催化剂载体,还可以有效地调节催化剂性能.本研究利用二维沸石IPC-1P作为铂纳米颗粒的载体,成功制备出异相催化剂Pt@IPC-1P.铂纳米颗粒的尺寸小于2 nm且均匀地分布在IPC-1P上.令人惊喜的是,Pt@IPC-1P催化剂对不饱和醇的选择性超过了90%,并实现了接近100%的催化转化率,远超其他铂基催化剂.理论计算证实,二维沸石IPC-1P在肉桂醛向肉桂醇的转化过程中起到了积极作用,其放热效应能量高于苯丙醛.这项工作展现了二维沸石在催化有机物转化领域具有广阔的应用前景.

关键词： heterogeneous catalysis 2D zeolite hydrogenation selectivity cinnamaldehyde top-down approach

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前列腺癌患者第二原发癌的研究进展

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肿瘤防治研究 2019年第6期46卷 567-569页

作者：崔庆鹏罗钰辉刘孝东昆明医科大学第一附属医院泌尿外科

前列腺癌是男性患者中发病率第二的肿瘤,且随着科技的发展,对前列腺癌的诊断和治疗手段越来越先进,显著延长了患者的生存时间,改善了患者的生存质量,从而也使得许多前列腺癌患者被检出继发第二原发癌。本文对前列腺癌患者常见的第二原... 详细信息

前列腺癌是男性患者中发病率第二的肿瘤,且随着科技的发展,对前列腺癌的诊断和治疗手段越来越先进,显著延长了患者的生存时间,改善了患者的生存质量,从而也使得许多前列腺癌患者被检出继发第二原发癌。本文对前列腺癌患者常见的第二原发癌及第二原发癌的病因、诊断、治疗和预后相关方面的研究进展予以综述,为该疾病的临床防治提供参考。

关键词：前列腺癌第二原发癌直肠癌肺癌膀胱癌

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