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生物物理学报 2009年第S1期25卷 55-55页

作者： yuhong xu molecular pharmaceutics lab, school of pharmacy, med-x research institute shanghai, jiaotong university.

The systemic delivery of nucleic acid drugs in vivo to transfect specific cell targets requires delivery systems with great serum stability and optimized pharmacokinetics

关键词： LMD Visualizing drug delivery case

Cloning,prokaryotic expression,purification,and functional verification of the insulin gene in black carp(Mylopharyngodon piceus)

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Aquaculture and Fisheries 2023年第1期8卷 18-25页

作者： Jiahua Zhang Yubang Shen Yafan Dai xiaoyan xu yuhong Su Jiale Li Key laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural AffairsShanghai Ocean UniversityShanghai201306China shanghai Engineering research Center of Aquaculture Shanghai Ocean UniversityShanghai201306China

In this paper,we studied the structure,expression and function of black carp insulin *** complete Mylopharyngodon piceus insulin(Mp-Ins)gene is 1,965 bp long and includes a 1,499 bp 5ʹ-untranslated region(UTR),a 139 bp 3′-UTR with a poly(A)tail,and an open reading frame(ORF)of 327 *** predicted molecular weight of the recombinant Mp-Ins(rMp-Ins)protein is 11.87 *** mRNA expression of Mp-Ins is upregulated in the brain and *** the injection of rMp-Ins,Mp-Ins mRNA transcript abundance was significantly upregulated in the *** rMp-Ins protein could inhibit the concentration of glycogen phosphorylase(GP),growth hormone(GH),fatty acid synthase(FAS),and insulin-like growth factors-1(IGF-1),and it also significantly increased the concentration of ***,the injection of rMp-Ins did not have a significant impact on the glucose-6-phosphatase(G6Pase)content in *** situ hybridization results showed that the positive signal of the Mp-Ins gene was mainly concentrated in the cell nucleus of brain tissue and the cell membrane of liver tissue and muscle ***,these results demonstrated that Mp-Ins plays an important role in growth and metabolism in ***.

关键词： Mylopharyngodon piceus Mp-Ins Prokaryotic expression Growth related Enzyme activity In situ hybridization

Identifying Qingkailing(清开灵)ingredients-dependent mesenchymal-epithelial transition factor-axiation“π”structuring module with angiogenesis and neurogenesis effects

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Journal of Traditional Chinese medicine 2024年第1期44卷 35-43页

作者： CHENG Kunming YUAN Jianan LIU Jun ZHANG Shengpeng xu Qixiang xIE Yong ZHAO Jingfeng ZHANG xiaoxu TANG xudong ZHENG Yongqiu WANG Zhong Provincial Engineering laboratory for Screening and Re-evaluation of Active Compounds of Herbal medicines in Southern Anhui Teaching and Research Section of Traditional Chinese MedicineSchool of PharmacyWannan Medical CollegeWuhu 241000China institute of Basic research in Clinical medicine China Academy of Chinese Medical SciencesBeijing 100700China Key laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal medicine Ministry of EducationDepartment of Medicinal Plant DevelopmentChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100193China Department of Gastroenterology Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing 100091China

OBJECTIVE:To explore the functional role of the drugdependent mesenchymal-epithelial transition(Met)-axiation"π"structural module of neurogenesis after processing by three components of Qingkailing injection in neurogenesis and angiogenesis in cerebral ***:We used a Glutathione S-transferase(GST)-pull down assay,isothermal titration calorimetry assay,and other related methods to identify the relationships among Met,inositol polyphosphate phosphatase like 1(Inppl1),and death associated protein kinase 3(Dapk3)in this allosteric *** biological effects of the modules of neurons generation composed of Met,Inppl1,and Dapk3 were measured through Western blot,apoptosis analysis,and double immunofluorescence ***:The GST-pull down assay revealed that proline-serine-threonine rich domain of Met binds to the Src homology domain of Inppl1 to form a protein-protein complex;Dapk3 with a C-terminal domain interacts weakly with the protein kinase C domain of Met in the intracellular ***,we obtained a“π”structuring module considered a neural regeneration *** biological effects of angiogenesis and neurogenesis modules composed of Met,Inppl1,and Dapk3 were also ***:The study suggested that understanding the functional modules that contribute to pharmaceutics might provide novel signatures that can be used as endpoints to define disease processes under stroke or cerebral ischemia conditions.

关键词： brain ischemia stroke neurogenesis angiogenesis modular pharmacology Met-axiation allosteric module

Separation of chitin from shrimp shells enabled by transition metal salt aqueous solution and ionic liquid

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Chinese Journal of Chemical Engineering 2023年第1期53卷 133-141页

作者： Mi Feng Bin He xinyan Chen Junli xu xingmei Lu Cai Jia Jian Sun Key laboratory of molecular medicine and Biotherapy Ministry of Industry and Information TechnologySchool of Life SciencesBeijing Institute of TechnologyBeijing 100081China Key laboratory of medical Molecule Science and pharmaceutics Engineering Ministry of Industry and Information TechnologyAdvanced Research Institute of Multidisciplinary ScienceBeijing Institute of TechnologyBeijing 100081China CAS Key laboratory of Green Process and Engineering State Key Laboratory of Multiphase Complex SystemsState Key Laboratory of Biochemical EngineeringBeijing Key Laboratory of Ionic Liquids Clean ProcessInstitute of Process EngineeringChinese Academy of SciencesBeijing 100190China school of Chemical and Engineering University of Chinese Academy of SciencesBeijing 100049China Innovation Academy for Green Manufacture Chinese Academy of SciencesBeijing 100190China

Chitin is a widely used important industrial polymer mainly from shrimp shells, but its commercial preparation is under the great challenge of serious pollution due to the requirement of HCl and Na ***, we demonstrated that high purity chitin can be obtained from waste shrimp shells(WSSs) by cascade separation with transition metal salt aqueous solution and ionic liquid(IL). Firstly, calcium carbonate of WSSs was effectively removed in the metal salt aqueous solution driven by the ion exchange interaction. Subsequently, 1-butyl-3-methylimidazolium chloride([Bmim]Cl) had bifunctional abilities to remove residual protein and introduced metal salts simultaneously by hydrogen bonding and coordination interactions. The key experimental factors affecting the separation process were systematically studied, including the type of metal salts, temperature, and [Bmim]Cl loading. After sequential treatment with a 20%(mass) Ni SO4aqueous solution at 130 ℃ and [Bmim]Cl at 150 ℃, the purity of a-chitin can be up to 96.5%(mass) that meets commercial requirements. The use of metal salts with higher coordination ability makes the preparation of chitin no longer depend on the commonly acid-base reaction, which is conducive to the preservation of chitin structure.

关键词： Chitin Ionic liquids Transition metal salts Separation Sustainability

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石蒜光合特性比较及其对温度胁迫的响应

分子植物育种 2020年第18期18卷 6165-6173页

作者：朱玲张旭张姣佼郑玉红周坚南京林业大学生物与环境学院南方现代林业协同创新中心南京210037 江苏省原子医学研究所江苏省分子核医学重点实验室无锡214063 江苏省中国科学院植物研究所(南京中山植物园) 观赏植物研究中心南京210014

为寻找适合林下栽培的石蒜种源,本研究通过比较19个种源石蒜光合参数,筛选出高光效种源,并进一步对该种源温度胁迫的响应机制进行分析。结果表明:在19个地理种源石蒜中,南京、黄山种源各项光合参数均处于优势水平,可作为高光效种源扩大... 详细信息

为寻找适合林下栽培的石蒜种源,本研究通过比较19个种源石蒜光合参数,筛选出高光效种源,并进一步对该种源温度胁迫的响应机制进行分析。结果表明:在19个地理种源石蒜中,南京、黄山种源各项光合参数均处于优势水平,可作为高光效种源扩大生产。其中,南京种源在高温和低温胁迫下,Pn均降低,高温胁迫下Tr始终高于胁迫初期水平,在低温胁迫下Tr表现为不可逆的降低趋势。石蒜ACBP6基因受温度诱导,高温胁迫下的表达量显著高于低温胁迫下。在两种温度胁迫下,ABA含量均增加,但短期的胁迫中低温诱导的ABA浓度更高。石蒜的ACBP6基因和内源激素ABA可能参与了其对温度胁迫的防御反应。本研究为石蒜适宜种源筛选及温度胁迫响应机制研究提供理论依据。

关键词：石蒜(Lycoris radiata(L'Her.)Herb.) 种源光合作用温度胁迫 ACBP6

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线型酚醛树脂结构改性的研究进展

材料导报 2023年第23期37卷 205-214页

作者：陈双雷子萱徐力李嘉玄陈栋梁强军锋刘育红西安科技大学材料科学与工程学院西安710054 西安交通大学化学工程与技术学院西安710049

酚醛树脂具有良好的力学性能和耐高温性,被广泛应用于航天、建筑等多个领域,尤其作为高性能碳/碳复合材料或碳/酚醛耐烧蚀材料的基体树脂。传统酚醛树脂固化过程释放挥发分,固化后交联结构存在不足,如紧密堆砌的刚性芳环导致其脆性高,... 详细信息

酚醛树脂具有良好的力学性能和耐高温性,被广泛应用于航天、建筑等多个领域,尤其作为高性能碳/碳复合材料或碳/酚醛耐烧蚀材料的基体树脂。传统酚醛树脂固化过程释放挥发分,固化后交联结构存在不足,如紧密堆砌的刚性芳环导致其脆性高,易氧化的酚羟基与亚甲基降低了其耐热性和残炭率。传统酚醛的多层次结构设计和改性是满足酚醛树脂在新型复合材料成型工艺和高性能热防护领域应用的关键,例如在线型酚醛分子结构中引入可加成官能团,如炔丙基、烯丙基等,借助此类基团之间的热聚合实现酚醛树脂的加成固化,还可以对酚醛树脂进行结构设计改性,如羟基醚化、环氧化或引进钼、硼、有机硅等组分,通过化学键接和物理缠结实现对分子结构的调控。这类新型酚醛树脂从本质上避免了固化过程中小分子的逸出,更易于制备出高质量的复合材料。鉴于此,本文着重叙述了几种典型的结构改性线型酚醛树脂及其在烧蚀防护领域的研究和应用进展。

关键词：酚醛树脂结构改性加成固化耐热复合材料

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水茄果实总RNA提取方法的比较分析

分子植物育种 2016年第4期14卷 864-869页

作者：秦亚龙王忠徐敏郑玉红束晓春彭峰江苏省中国科学院植物研究所南京210014

水茄果实中富含多糖、蛋白、色素和其他代谢物质,影响其总RNA提取的质量。本研究中比较Trizol法、改良Trizol法、改良CTAB法和两种试剂盒法5种方法提取的水茄果实总RNA,用琼脂糖凝胶电泳检测总RNA的完整性,Onedrop光谱仪检测总RNA的浓... 详细信息

水茄果实中富含多糖、蛋白、色素和其他代谢物质,影响其总RNA提取的质量。本研究中比较Trizol法、改良Trizol法、改良CTAB法和两种试剂盒法5种方法提取的水茄果实总RNA,用琼脂糖凝胶电泳检测总RNA的完整性,Onedrop光谱仪检测总RNA的浓度和纯度。结果表明:改良Trizol法最为有效,该方法提取的总RNA在琼脂糖凝胶上得到清晰的28S和18S条带,OD260nm/OD280nm值在1.82.1之间、OD260nm/OD230nm的比值大于2.0;改良CTAB法获得的RNA质量较好,但步骤较为繁琐,耗时长;Trizol法和试剂盒法得到总RNA浓度较低。RT-PCR试验进一步表明,以改良Trizol法获得的总RNA可用于基因分离,所得条带清晰,与目的片段大小一致且比对正确。

关键词：水茄果实总RNA 提取方法改良Trizol法

An infection-microenvironment-targeted and responsive peptide-drug nanosystem for sepsis emergency by suppressing infection and inflammation

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Asian Journal of Pharmaceutical Sciences 2023年第6期18卷 126-143页

作者： Wei He Daan Fu Yongkang Gai xingxin Liu Chang Yang Zhilan Ye xu Chen Jia Liu Bingcheng Chang The Second Clinical College The Second Affiliated HospitalGuizhou University of Traditional Chinese MedicineGuiyang 550003China research Center for Tissue Engineering and Regenerative medicine Tongji Medical CollegeUnion HospitalHuazhong University of Science and TechnologyWuhan 430022China Department of Anesthesiology Tongji Medical CollegeUnion HospitalHuazhong University of Science and TechnologyWuhan 430022China Department of laboratory medicine West China Second University HospitalSichuan UniversityChengdu 610065China Department of Nuclear medicine Hubei Province Key Laboratory of Molecular ImagingTongji Medical CollegeUnion HospitalHuazhong University of Science and TechnologyWuhan 430022China Guizhou Provincial Key laboratory of pharmaceutics Guizhou Medical UniversityGuiyang 550004China Department of Geriatrics Tongji Medical CollegeUnion HospitalHuazhong University of Science and TechnologyWuhan 430022China

Sepsis is a life-threatening emergency that causes millions of deaths every year due to severe infection and ***,current therapeutic regimens are inadequate to promptly address the vast diversity of potential ***,an antimicrobial peptide,has shown promise for neutralizing endotoxins and eliminating diverse ***,its clinical application is hindered by safety and stability ***,we present a nanoscale drug delivery system(Omi-hyd-Dex@HA NPs)that selectively targets infectious microenvironments(IMEs)and responds to specific stimuli for efficient intervention in *** system consists of omiganan-dexamethasone conjugates linked by hydrazone bonds which self-assemble into nanoparticles coated with a hyaluronic acid(HA).The HA coating not only facilitates IMEs-targeting through interaction with intercellular-adhesion-molecule-1 on inflamed endotheliocytes,but also improves the biosafety of the nanosystem and enhances drug accumulation in primary infection sites triggered by *** nanoparticles release dual drugs in IMEs through pH-sensitive cleavage of hydrazone bonds to eradicate pathogens and suppress *** multiple tissue infection and sepsis animal models,Omi-hyd-Dex@HA NPs exhibited rapid source control and comprehensive inflammation reduction,thereby preventing subsequent fatal complications and significantly improving survival *** bio-responsive and self-delivering nanosystem offers a promising strategy for systemic sepsis treatment in emergencies.

关键词： Infectious microenvironments Sepsis Nanoscale drug delivery systems Pathogens Omiganan

医学分子生物学CRISPR基因编辑与疾病医疗的社会变革

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医学与社会 2019年第6期32卷 30-32,49页

作者：李忠光孙渝婷史梦婷杨蓉王丹梁瑜于秋霞王泽帆王娟任珂星王李阳周鑫 MengMeng xu William Isaacs Jianjie Ma 徐学红陕西师范大学生命科学学院细胞遗传及发育生物学西安710119 Ohio State university.school of medicine Columbus BID medical Center Harvard Medical SchoolBoston Duke university.medical Center Durham Johns Hopkins university.school of medicine BaltimoreMD21287USA

利用CRISPR(规律成簇间隔短回文重复序列)技术体系进行基因精密编辑,从基因分子医学层面对疾病进行高效治疗已成为现代医学研究的重要方向,并在临床应用上将有重要的突破。本文论述其近期临床医学研究对社会的相关影响,对CRISPR基因精... 详细信息

利用CRISPR(规律成簇间隔短回文重复序列)技术体系进行基因精密编辑,从基因分子医学层面对疾病进行高效治疗已成为现代医学研究的重要方向,并在临床应用上将有重要的突破。本文论述其近期临床医学研究对社会的相关影响,对CRISPR基因精密编辑的知识产权归属问题进行了分析。以哈佛大学“精准基因修饰干细胞计划”为题探讨了该技术体系在人类遗传性疾病治疗中的应用,在器官移植上的成功将对社会产生重要的影响。CRISPR技术上的正负向高通量筛选,成功地捕获到黑素瘤的药物维罗非尼耐受敏感的关键基因,为解决这一问题打下了重要的基础。CRISPR基因精密编辑的应用已成为生物医学发展的趋势,分析其对人类健康的影响,有助于掌控这一历史性变革技术,为人类社会发展做出重要贡献。

关键词：精准基因编辑 CRISPR/Cas基因编辑干细胞药物免疫耐受