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芍药PlSPL1基因克隆与表达特性分析

华北农学报 2024年第2期39卷 55-61页

作者：许华杰卢莉莉汤寓涵赵大球孟家松陶俊扬州大学园艺园林学院江苏扬州225009 扬州大学教育部农业与农产品安全国际联合实验室江苏扬州225009

为研究芍药PlSPL1(SPL)基因的性质和功能,进一步阐明PlSPL1基因在不同物种中的共性与特征差异,探明PlSPL1在芍药茎秆挺直程度中的作用。以芍药红峰茎秆为材料,采用RACE技术获得了芍药PlSPL1基因全长序列,利用生物信息学软件分析预测PlS... 详细信息

为研究芍药PlSPL1(SPL)基因的性质和功能,进一步阐明PlSPL1基因在不同物种中的共性与特征差异,探明PlSPL1在芍药茎秆挺直程度中的作用。以芍药红峰茎秆为材料,采用RACE技术获得了芍药PlSPL1基因全长序列,利用生物信息学软件分析预测PlSPL1的结构、理化性质和亲缘关系,随后利用qRT-PCR技术分析PlSPL1在芍药茎秆不同发育时期的表达量,并通过激光共聚焦扫描显微技术进行了蛋白质亚细胞定位分析。结果表明:PlSPL1基因开放阅读框为3000 bp,编码999个氨基酸。蛋白分子式为C_(4869)H_(7682)N_(1406)O_(1497)S_(43),分子量为111.25 ku,理论等电点为6.26,编码亲水性不稳定酸性蛋白,磷酸化修饰以丝氨酸为主,无信号肽,有跨膜结构,二级结构主要由无规则卷曲组成。系统进化树分析发现,PlSPL1蛋白与牡丹的亲缘关系最近,其次和葡萄有较近的亲缘关系;蛋白序列比对分析发现,PlSPL1蛋白具有SPL转录因子家族特有的SBP保守结构域。相对表达量分析发现,PlSPL1随着茎秆发育逐渐呈现下降趋势,表明PlSPL1负向调控芍药茎秆发育,推测其可能在茎秆挺直程度方面起重要作用;亚细胞定位显示,PlSPL1蛋白定位在细胞核中。上述结果表明,PlSPL1参与芍药茎秆发育过程。

关键词：芍药 SPL 基因克隆表达模式亚细胞定位

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Sensitivity-Enhanced Hot-Wire Anemometer by Using Cladding-Etched Fiber Bragg Grating

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Photonic Sensors 2023年第3期13卷 1-7页

作者： yuhan tang Xuke CHEN Jiarui ZHANG Dajuan LV Liangming XIONG Xinyong DONG School of Physics and Optoelectronic Engineering Guangdong University of TechnologyGuangzhou 510006China Guangdong Provincial Key Laboratory of Information Photonics Technology Guangzhou 510006China Institute of Optoelectronic Technology China Jiliang UniversityHangzhou 310018China School of Information Engineering Guangdong University of TechnologyGuangzhou 510006China State Key Laboratory of Optical Fiber and Cable Manufacture Technology Wuhan 430073China Southern Marine Science and Engineering Guangdong Laboratory Zhuhai 519000China

A sensitivity-enhanced hot-wire anemometer based on a cladding-etched optical fiber Bragg grating(FBG)coated with a layer of silver film and optically heated by using a 1480nm laser diode is *** silver film absorbs the laser power to heat the FBG to a certain high temperature and the airflow cools down the FBG hot-wire with the cooling effect and hence the Bragg wavelength of the FBG is determined by the airflow *** measurement results show that the heating efficiency of the FBG hot wire is improved by 3.8times in magnitude by etching the fiber cladding from 125μm down to 73.4μm,and the achieved airflow velocity sensitivities,under a laser power of 200mW,are–3180pm/(m/s),–889pm/(m/s),–268pm/(m/s),and–8.7pm/(m/s)at different airflow velocities of 0.1m/s,0.5m/s,1.5m/s,and 17m/s,*** comparison,the sensitivities are only–2193 pm/(m/s),–567 pm/(m/s),–161 pm/(m/s),and–4.9pm/(m/s)for the reference anemometer without cladding etching even at a much higher heating laser power of *** results prove that the method by using a cladding-etched FBG to improve sensitivity of FBG-based hot-wire anemometers works and the sensitivity is improved significantly.

关键词： Fiber Bragg grating hot-wire anemometer optical fiber sensors

姜黄素联合富硒酵母对小鼠急性酒精性肝损伤的保护作用

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食品安全导刊 2024年第9期 48-53页

作者：梁婵华莫丽田重阳唐玉涵华中科技大学同济医学院公共卫生学院湖北武汉430030

目的:探讨姜黄素联合富硒酵母对急性酒精性肝损伤的保护作用。方法:将60只C57B6L/J小鼠随机分为空白组、模型组、姜黄素组(33.33 mg·kg^(-1) bw)、富硒酵母组(7.50 mg·kg^(-1) bw)和联合组(姜黄素33.33 mg·kg^(-1) bw+富硒酵母7.50 m... 详细信息

目的:探讨姜黄素联合富硒酵母对急性酒精性肝损伤的保护作用。方法:将60只C57B6L/J小鼠随机分为空白组、模型组、姜黄素组(33.33 mg·kg^(-1) bw)、富硒酵母组(7.50 mg·kg^(-1) bw)和联合组(姜黄素33.33 mg·kg^(-1) bw+富硒酵母7.50 mg·kg^(-1) bw),每组12只;干预30 d后,一次性灌胃50%乙醇溶液(12 mL·kg^(-1) bw)建立急性酒精性肝损伤模型;观察小鼠体重、摄食、行为变化;检测小鼠肝脏指数与丙二醛(Malondialdehyde,MDA)、还原型谷胱甘肽(Glutathione,GSH)和甘油三酯(Triglyceride,TG)水平;油红染色观察小鼠肝脏组织病理学变化。结果:与空白组比较,模型组小鼠表现出肝脏肿大、肝细胞中脂滴大量堆积病理损伤,肝脏组织MDA和TG水平显著升高(P<0.05);姜黄素联合富硒酵母能显著降低小鼠肝脏中MDA和TG表达,提高GSH水平(P<0.05),减轻小鼠肝脏脂肪沉积。结论:姜黄素联合富硒酵母能够改善急性酒精灌胃诱导的肝脏脂质异常堆积,减轻氧化应激水平,对急性酒精性肝损伤具有良好的保护作用。

关键词：姜黄素富硒酵母急性酒精性肝损伤

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Phyton-International Journal of Experimental Botany 2022年第9期91卷 2005-2016页

作者： Yanqing Wu Jiao Liu yuhan tang Daqiu Zhao Jun Tao Joint International Research Laboratory of Agriculture and Agri-Product Safety The Ministry of Education of ChinaInstitutes of Agricultural Science and Technology DevelopmentYangzhou UniversityYangzhou225009China Jiangsu Key Laboratory of Crop Genetics and Physiology College of Horticulture and Plant ProtectionYangzhou UniversityYangzhou225009China

The ornamental and commercial values of herbaceous peony(Paeonia lactiflora Pall.)are directly related to its flower ***,the molecular mechanisms underlying the type formation of *** flowers have not been studied in great *** studies identified,using integrated multipleomics analysis,revealed that APETALA2(AP2)is an important candidate gene that modulates type formation of *** *** further reveal the expression mechanism of AP2 in *** petals,we examined the profile of AP2 expression in the inner and outer petals of‘ZiFengyu’at various developmental stages using qRT-PCR and BSP+Miseq methylation *** on our data,the AP2 levels in the outer petals were obviously increased,relative to the inner *** addition,the S3 levels at the bloom stage were significantly higher than at the flower-bud stage S1,thereby promoting bloom stage S2,while declining stage *** chromosome walking,the 2000 bp of the 5′-end upstream promoter region was *** region harbored a CpG island(−665∼−872 bp),with multiple essential transcription factor binding sites(TFBS)such as NF-kappa B,GATA-1,Sp1,and C/*** sequencing revealed 7 methylated CpG sites in the CpG island region of the AP2 promoter,thereinto,the methylation ratio of the CpG-3 site in the inner petals was significantly higher than in the outer *** analysis revealed a negative association between the level of methylation(CpG-3,CpG-6),and AP2 mRNA ***-3 was located on the Sp1 transcription factor binding ***,we speculated that the CpG-3 methylation may inhibit transcription factor Sp1 binding to the gene promoter,thereby regulating AP2 ***,we examined the role of AP2 in the determination of flower patterns in *** conclusion will provide theoretical guidance for the molecular breeding of the flower pattern in ***.

关键词： Herbaceous peony APETALA2(AP2) developmental expression DNA methylation

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铜死亡在肿瘤中的研究进展

实用肿瘤学杂志 2024年第1期38卷 50-54页

作者：高源唐钰涵王露(综述) 赵文辉(审校) 哈尔滨医科大学附属肿瘤医院乳腺肿瘤内科一科室哈尔滨150081

铜是生物体内不可缺少的一种微量元素,生理状态下在细胞内维持一定的水平。铜死亡是一种独特、铜依赖性的细胞死亡,是极为重要的一种调节性细胞死亡(Regulated cell death,RCD),且不同于现阶段已知的其他细胞死亡形式。越来越多的研究表... 详细信息

铜是生物体内不可缺少的一种微量元素,生理状态下在细胞内维持一定的水平。铜死亡是一种独特、铜依赖性的细胞死亡,是极为重要的一种调节性细胞死亡(Regulated cell death,RCD),且不同于现阶段已知的其他细胞死亡形式。越来越多的研究表明,铜死亡对肿瘤细胞的代谢途径转换、信号通路传导、蛋白质的加工与修饰均有着不可忽视的影响。铜死亡相关lncRNA也被广泛用于预测肿瘤的预后。铜死亡可以抑制肿瘤细胞生长,并可作为控制耐药和疾病复发的潜在靶点。本文对铜死亡在肿瘤发生发展中的作用机制进行综述。

关键词：铜死亡肿瘤细胞死亡调节性细胞死亡

Lignin provides mechanical support to herbaceous peony(Paeonia lactiflora Pall.)stems

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Horticulture Research 2020年第1期7卷 14-28页

作者： Daqiu Zhao Yuting Luan Xing Xia Wenbo Shi yuhan tang Jun Tao College of Horticulture and Plant Protection Yangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety The Ministry of Education of ChinaYangzhou UniversityYangzhou 225009P.R.China

Stem bending caused by mechanical failure is a major constraint for high-quality herbaceous peony(Paeonia lactiflora Pall.)cut flowers,but little is known about the underlying *** this study,two *** cultivars,Xixia Yingxue(bending)and Hong Feng(upright),were used to investigate differences in stem *** results showed that the stem mechanical strength of Hong Feng was significantly higher than that of Xixia Yingxue,and the thickening of the secondary cell wall and the number of thickened secondary cell wall layers in Hong Feng were significantly higher than those in Xixia ***,compared with Xixia Yingxue,Hong Feng showed greater lignification of the cell wall and lignin deposition in the cell walls of the sclerenchyma,vascular bundle sheath and *** three types of lignin monomers were *** S-lignin,G-lignin,and total lignin contents and the activities of several lignin biosynthesis-related enzymes were higher in Hong Feng than in the other cultivar,and the S-lignin content was closely correlated with stem mechanical *** addition,113,974 full-length isoforms with an average read length of 2106 bp were obtained from the full-length transcriptome of *** stems,and differential expression analysis was performed based on the comparative transcriptomes of these two *** lignin biosynthesis-related genes,including 26 members that were closely associated with lignin content,were identified,and multiple upregulated and downregulated transcription factors were found to positively or negatively regulate lignin ***,lignin was shown to provide mechanical support to *** stems,providing useful information for understanding the formation of *** stem strength.

关键词： strength flowers cultivar

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基于FKM标准的转向架构架疲劳强度评估

电力机车与城轨车辆 2024年第1期47卷 57-61,65页

作者：唐毓晗王悦东董祺刘嘉禹温珈毅大连交通大学机车车辆工程学院辽宁大连116028 中国铁路沈阳局集团有限公司沈阳动车段辽宁沈阳110002

随着车辆速度的提升及振动程度的加剧,转向架焊接部位极易发生疲劳破坏,因此选用较为科学的疲劳评估标准非常重要。文章以某高速动车组拖车转向架构架为例,综合UIC 515-4和EN 13749标准要求进行超常载荷和模拟运营载荷等工况分析,依据... 详细信息

随着车辆速度的提升及振动程度的加剧,转向架焊接部位极易发生疲劳破坏,因此选用较为科学的疲劳评估标准非常重要。文章以某高速动车组拖车转向架构架为例,综合UIC 515-4和EN 13749标准要求进行超常载荷和模拟运营载荷等工况分析,依据分析结果选取疲劳评估点,并分别应用FKM标准和修正的Goodman-Smith曲线进行疲劳强度评估和对比分析。结果表明:FKM标准可应用于轨道车辆各结构的疲劳强度评估,且评估过程更为科学合理,评估结果更为保守可靠。

关键词：高速动车组转向架构架 FKM标准 Goodman-Smith曲线疲劳强度

Deterioration of Antioxidant Competence in Barley Lesion Mimic Mutant 194

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Phyton-International Journal of Experimental Botany 2019年第2期88卷 109-117页

作者： Qunqun Hao Bo Lyu yuhan tang Deya Wang Yuanyuan Li Qingliang Li Yuhai Wang Wenqiang Wang College of Life Sciences Zaozhuang UniversityZaozhuang277000China State Key Laboratory of Crop Biology College of AgronomyShandong Agricultural UniversityTai’an271018China.

A barley mutant,194,was observed to exhibit a leaf spot phenotype over the whole course of its growing *** this study,the phenotype and antioxidant competence were studied in the lesion mimic mutant *** height was slightly higher in mutant 194 than in the wild type(WT).In addition,leaf spot per plant in mutant 194 was significantly higher than in *** competence,as indicated by reactive oxygen species(ROS)accumulation,antioxidant enzyme activity,and the expression of antioxidant enzyme-encoding genes was also assessed in mutant *** to the WT,mutant 194 displayed a relatively higher accumulation of ROS,accompanied by lower activities of some antioxidant enzymes and downregulation of antioxidant enzyme-encoding *** demonstrated reduced antioxidant competence in mutant *** results suggested that this lower antioxidant competence of mutant 194 could lead to the accumulation of excessive *** excess of ROS could induce programmed cell death and has the potential to promote disease resistance in mutant 194.

关键词： Lesion mimic mutant ROS accumulation antioxidant enzymes barley

Reduced exciton binding energy and diverse molecular stacking enable high-performance organic solar cells with V_(OC)over 1.1 V

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Science China Chemistry 2024年第9期67卷 3140-3152页

作者： Tingting Dai Jiahao Lu Ailing tang yuhan Meng Peiqing Cong Zongtao Wang Erjun Zhou National Center for Nanoscience and Technology Beijing 100190China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China College of Chemistry and Chemical Engineering Guangxi Minzu UniversityNanning 530006China Henan Institute of Advanced Technology Zhengzhou UniversityZhengzhou 450003China

High-voltage organic solar cells(OSCs)have received increasing attention because of their promising applications in tandem devices and indoor photovoltaics,but the trade-off between energy loss and charge generation induced by exciton binding energy(E_(b))has become one of the biggest bottlenecks limiting the development of this ***,a wide bandgap(WBG)nonfullerene acceptor BTA503 with reduced E_(b) is designed by changing the phenyl side chain on the central core of Cl-BTA5 to an alkyl *** diverseπ-πinteractions and enhanced molecular stacking of BTA503 are responsible for its reduced E_(b).Furthermore,both the diminished charge recombination and the fast exciton dissociation caused by the small E_(b) favor the generation of more charge carriers for the PTQ10:BTA503 *** efficient Forster resonance energy transfer(FRET)and multiple π-π stacking patterns provide additional charge transfer and transport ***,the PTQ10:BTA503-based OSC device achieves a V_(OC)of 1.112 V and a PCE of 12.70%,which is higher than that of PTQ10:Cl-BTA5(PCE=10.92%).Simultaneously,the thick film(~300 nm)binary device of PTQ10:BTA503 achieves a PCE of 10.13% with a V_(OC)of 1.102 V,which is the best result for thick film high-voltage *** importantly,the ternary device of PTQ10:BTA503:Cl-BTA5(1:0.9:0.1)realizes a champion PCE of 13.12% with a V_(OC)of 1.126 *** study demonstrates that it is an effective strategy to reduce E_(b) of A_(2)-A_(1)-D-A_(1)-A_(2) type WBG acceptors by modulating the side chains on D unit,which further favors the corresponding devices to obtain world-record PCE and improves their potential for commercial applications.

关键词： exciton binding energy intermolecular interaction wide-bandgap acceptor high V_(OC) organic solar cells